一个大豆矮秆性状基因的分子标记定位
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摘要
【目的】对大豆品种北农103中的矮秆性状进行遗传分析和初步基因定位,为该基因的精细定位和育种利用奠定基础。【方法】以高秆大豆品种海系13和矮秆品种北农103为研究材料,通过对(海系13×北农103)F4的剩余杂合体(RHL)F2分离群体的遗传分析,解析控制北农103矮秆性状基因的遗传规律,同时以(海系13×北农103)的(RHL)F2∶3为作图群体,利用BSA法结合SSR分子标记,对北农103中的矮秆基因进行分子标记和遗传定位。【结果】北农103中携带1对控制矮秆性状的隐性基因GmD1;经分子标记连锁分析,该基因与9个SSR标记连锁,分别是Satt527、Satt166、Sat_340、Satt481、Sat_150、Sat_245、Satt076、Satt229和Satt664。【结论】根据大豆遗传图谱推断,该基因位于大豆L连锁群(19染色体)上,在satt527和satt166标记之间,遗传距离分别是3.7cM和4.2cM,推测为一个新的控制大豆矮秆的基因GmD1,这些发现和分子标记定位对于该基因的精细定位将起到重要作用。
【Objective】The dwarf soybean variety BeiNong 103 was researched by genetic analysis and preliminary gene localization for fine mapping and breeding utilization.【Methods】To genetic analysis and gene mapping,a F2:4 segregating population derived from a cross of high-stalk cultivar HaiXi 13 with a dwarf-stalk accession BeiNong 103.And the BSA methods was used as SSR primers screening and gene localization.【Results】Genetic analysis showed that the dwarf trait of BeiNong 103 was controlled by a recessive gene GmD1.Satt527,Satt166,Sat_340,Satt481,Sat_150,Sat_245,Satt076,Satt229 and Satt664,9 SSR markers were found linkaged with the GmD1 gene respectively.【Conclusion】According to the gene location,the GmD1 gene was located on the soybean L-linked group(chromosome 19),and the genetic distance were 3.7 cM 和4.2 cM between the satt527 and satt166.The speculate that GmD1 was a new dwarf gene of soybean.The results provide important information for the fine mapping of genes.
【Objective】The dwarf soybean variety BeiNong 103 was researched by genetic analysis and preliminary gene localization for fine mapping and breeding utilization.【Methods】To genetic analysis and gene mapping,a F2:4 segregating population derived from a cross of high-stalk cultivar HaiXi 13 with a dwarf-stalk accession BeiNong 103.And the BSA methods was used as SSR primers screening and gene localization.【Results】Genetic analysis showed that the dwarf trait of BeiNong 103 was controlled by a recessive gene GmD1.Satt527,Satt166,Sat_340,Satt481,Sat_150,Sat_245,Satt076,Satt229 and Satt664,9 SSR markers were found linkaged with the GmD1 gene respectively.【Conclusion】According to the gene location,the GmD1 gene was located on the soybean L-linked group(chromosome 19),and the genetic distance were 3.7 cM 和4.2 cM between the satt527 and satt166.The speculate that GmD1 was a new dwarf gene of soybean.The results provide important information for the fine mapping of genes.
引文
[1]卢媛,崔超凡,胡平,陈佩度,沈雪芳,韩晴,王义发,邢莉萍,曹爱忠.矮秆基因Rht_NM9在小麦株高建成中对内源激素含量的影响[J].作物学报,2017(9):1272-1279
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[10]Kilen T C.Inheritance of a brachytic character in soybeans[J].Crop Science,1977,17(6):853-854
[11]Hwang W J,Kim M Y,Kang Y J,Shim S,Stacey M G,Stacey G,Lee Soi.Genome-wide analysis of mutations in a dwarfsoybean mutant induced by fast neutron bombardment[J].Euphytica,2015,203(2):399-408
[12]Cheng W,Gao J S,Feng X X,Shao Q,Yang S X,Feng XZ.Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean[J].Journal of Integrative Agriculture,2016,15(10):2228-2236
[13]Li Z F,Guo Y,Ou L,Hong HL,Wang J,Liu Z X,Guo BF,Zhang L j,Qiu L j.Identification of the dwarf gene GmDW1in soybean(Glycine max L.)by combining mappingby-sequencing and linkage analysis[J].Theoretical and Applied Genetics,2018,131(5):1001-1016
[2]嵇怡,缪旻珉,陈学好.植物矮生性状的分子遗传研究进展[J].分子植物育种,2006,4(6):753-771
[3]魏国才,常大军,刘淑玲.矮秆玉米及其应用前景[J].现代化农业,1999(8):24-25
[4]邱丽娟,郭勇,黎裕.中国作物新基因发掘:现状、挑战与展望[J].作物学报,2011(1):1-17
[5]Cooper R L.Breeding semidwarf soybean[M].Westport Connecticut:AVI Publishing Company Incorporation,1985
[6]曹永强,谢甫绨,张惠君,王海英,宋书宏,王文斌.大豆不同亲本正交和反交后代株高遗传规律的比较研究[J].辽宁农业科学,2006(4):13-15
[7]杨琪,王金陵.不同类型大豆双列杂交遗传参数分析[J].中国农业科学,1995,28(A01):76-80
[8]陈恒鹤.大豆矮秆基因及其育种改良效果的研究[J].作物学报,1985,11(2):95-102
[9]Kilen T C,Hartwig E E.Short internode character in soybean and its inheritance[J].Crop Science,1975,15(6):878-879
[10]Kilen T C.Inheritance of a brachytic character in soybeans[J].Crop Science,1977,17(6):853-854
[11]Hwang W J,Kim M Y,Kang Y J,Shim S,Stacey M G,Stacey G,Lee Soi.Genome-wide analysis of mutations in a dwarfsoybean mutant induced by fast neutron bombardment[J].Euphytica,2015,203(2):399-408
[12]Cheng W,Gao J S,Feng X X,Shao Q,Yang S X,Feng XZ.Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean[J].Journal of Integrative Agriculture,2016,15(10):2228-2236
[13]Li Z F,Guo Y,Ou L,Hong HL,Wang J,Liu Z X,Guo BF,Zhang L j,Qiu L j.Identification of the dwarf gene GmDW1in soybean(Glycine max L.)by combining mappingby-sequencing and linkage analysis[J].Theoretical and Applied Genetics,2018,131(5):1001-1016