安特逍胶囊对Beagle犬39周重复给药毒性实验研究
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摘要
目的观察连续灌胃给予安特逍胶囊对Beagle犬产生的毒性作用。方法 Beagle犬48只,分为安特逍胶囊120、320、800 mg·kg~(-1)剂量组和溶媒对照组,分别灌胃给予相应药物或溶媒,每天1次,连续39周,检查其一般体征、体重、心电图、血液学、血清生化学、脏器系数、组织病理学等指标。结果安特逍胶囊给药可引起动物呕吐、流涎、稀便等症状;800 mg·kg~(-1)剂量可导致动物体重下降(P <0.05);320和800 mg·kg~(-1)剂量可引起血清TP、ALB和GLOB等指标发生变化(P <0.05);组织病理学检查未见与药物相关的病变。结论灌胃给予安特逍胶囊320和800 mg·kg~(-1)剂量对Beagle犬具有一定胃肠道毒性。
Objective To examine the repeated dose toxicity of Antexiao capsule in Beagle dogs. Methods 48 Beagle dogs were divided into four groups: 120, 320, 800 mg·kg~(-1) dose of Antexiao capsule groups and solvent control group.The dogs were orally treated with Antexiao capsule for 39 weeks. Clinical observation, body weight, electrocardiogram,hematology, blood biochemistry, organ weights, and histopathological findings were examined. Results Antexiao capsule could induce vomiting, salivation and liquid stools in healthy dogs. The body weight of dogs in 800 mg·kg~(-1) group decreased significantly(P <0.05). The levels of serum TP, ALB and GLOB changed in dogs treated with 320 and800 mg·kg~(-1) Antexiao capsule. No significant drug-related pathological changes were found. Conclusion Antexiao capsule(320 and 800 mg·kg~(-1)) administered intragastrically can induce significant gastrointestinal toxicity in Beagle dogs.
Objective To examine the repeated dose toxicity of Antexiao capsule in Beagle dogs. Methods 48 Beagle dogs were divided into four groups: 120, 320, 800 mg·kg~(-1) dose of Antexiao capsule groups and solvent control group.The dogs were orally treated with Antexiao capsule for 39 weeks. Clinical observation, body weight, electrocardiogram,hematology, blood biochemistry, organ weights, and histopathological findings were examined. Results Antexiao capsule could induce vomiting, salivation and liquid stools in healthy dogs. The body weight of dogs in 800 mg·kg~(-1) group decreased significantly(P <0.05). The levels of serum TP, ALB and GLOB changed in dogs treated with 320 and800 mg·kg~(-1) Antexiao capsule. No significant drug-related pathological changes were found. Conclusion Antexiao capsule(320 and 800 mg·kg~(-1)) administered intragastrically can induce significant gastrointestinal toxicity in Beagle dogs.
引文
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[15]李霞,孙慧萍,刘文霞,等.苜蓿总皂苷对Beagle犬的长期毒性实验研究[J].中国药物警戒, 2017, 14(6):331-333.
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[2] Mo X Q, Wei H Y, Huang G R, et al. Molecular mechanisms of apoptosis in hepatocellular carcinoma cells induced by ethanol extracts of Solanum lyratum Thumb through the mitochondrial pathway[J]. World J Gastroenterol,2017, 23(6):1010-1017.
[3]韩林,卓越,孙彩霞,等.白英抑瘤作用有效部位的初步筛选[J].中华中医药杂志, 2016, 31(9):3770-3774.
[4]卜,王建农,臧雅丽,等.白英甾体总生物碱抑制小鼠肝癌细胞H22移植瘤生长药效学研究[J].时珍国医国药, 2013, 24(7):1593-1595.
[5]王建农,臧雅丽,顾士萍,等.白英甾体总生物碱对人肺癌细胞A549裸鼠异种移植瘤的药效研究[J].中药新药与临床药理,2013, 24(5):469-472.
[6]王建农.一种白英提取物以及包括其的抗癌药物[P]:中国专利,CN102319319B. 2012-01-18.
[7]韩林,孙彩霞,王建农.白英甾体总生物碱通过VEGF相关信号通路调控A549细胞的凋亡与周期[J].中药新药与临床药理,2016, 27(4):509-513.
[8] Diaz G J. Toxicosis by Plant Alkaloids in Humans and Animals in Colombia[J]. Toxins. 2015, 7(12):5408-5416.
[9] Welch K D, Green B T, Gardner D R, et al. The effect of 7,8-methylenedioxylycoctonine-type diterpenoid alkaloids on the toxicity of tall larkspur(Delphinium spp.)in cattle[J]. J Anim Sci,2012, 90(7):2394-2401.
[10]刘玉勤,李慧杰,齐元富.齐元富运用蛇莓配伍白英治疗肿瘤经验[J].湖南中医杂志. 2014, 30(2):22-22.
[11]王剑,李玲香.加味白英汤防治鼻咽癌三维适型放疗后口腔干燥症的疗效分析[J].中国医师杂志, 2017, 19(5):750-751.
[12]韩林.白英生物碱对非小细胞肺癌的抑制作用及凝集脂筏胆固醇抑制血管新生的抗肿瘤机制研究[D].中国中医科学院, 2017.
[13]汪志铮.治癌良将-白英[J].现代养生, 2017, 5(9):25.
[14]严杰,罗菁.白英抗肿瘤研究进展[J].中外健康文摘:医药月刊,2007, 4(4):116-118.
[15]李霞,孙慧萍,刘文霞,等.苜蓿总皂苷对Beagle犬的长期毒性实验研究[J].中国药物警戒, 2017, 14(6):331-333.
[16]徐淑云.药理实验方法学[M].3版.北京:人民卫生出版社,2002:911.