高效液相色谱法测定橙汁中果糖含量的不确定度评定
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摘要
目的评定高效液相色谱法测定橙汁中果糖含量的不确定度。方法采用高效液相色谱法测定橙汁饮料中果糖的含量,建立数学模型,对检测过程中可能引入不确定度的因素包括样品的处理、标准溶液配制、标准曲线拟合及样品的重复测定进行分析及评定,最终合成橙汁中果糖含量测定结果的不确定度。结果橙汁中果糖含量测定结果是33.5 g/kg时,在95%的置信区间下,扩展不确定度为1.88 g/kg (k=2)。结论不确定度的主要来源是标准溶液配制和标准曲线拟合。
Objective To evaluate the uncertainty of the determination of fructose content in orange juice by high performance liquid chromatography(HPLC). Methods The content of fructose in orange juice was measured by HPLC, a mathematical model was established to analyze and evaluate the possible uncertainties of sample treatment, standard solution preparation, standard curve fitting and measurement repeatability, and the uncertainty of determination of fructose in orange juice was finally synthesized. Results When the fructose content was 33.5 g/kg,the extended uncertainty was 1.88 g/kg(k=2) under the 95% confidence interval. Conclusion The major factors of uncertainty are the standard solution preparation and the standard curve fitting.
Objective To evaluate the uncertainty of the determination of fructose content in orange juice by high performance liquid chromatography(HPLC). Methods The content of fructose in orange juice was measured by HPLC, a mathematical model was established to analyze and evaluate the possible uncertainties of sample treatment, standard solution preparation, standard curve fitting and measurement repeatability, and the uncertainty of determination of fructose in orange juice was finally synthesized. Results When the fructose content was 33.5 g/kg,the extended uncertainty was 1.88 g/kg(k=2) under the 95% confidence interval. Conclusion The major factors of uncertainty are the standard solution preparation and the standard curve fitting.
引文
[1]齐乐,祁春节.中国橙汁进口差异化需求弹性分析[J].世界农业,2017,(4):144-150.Qi L,Qi CJ.Elastic analysis of differential demand for Chinese orange juice imports[J].World Agric,2017,(4):144-150.
[2]USDA.Citrus:World markets and trade[EB/OL].[2018-5-16].http:www.fas.usda.gov/psdonline/circulars/citrus.pdf.
[3]曾祥国.不同种类和产区柑橘糖酸含量及组成研究[D].武汉:华中农业大学,2005.Zeng XG.Studies on contents and composition of sugar and acid in citrus from different species and production areas[D].Wuhan:Huazhong Agricultural University,2005.
[4]胡梦坤,岑琴,李丹.果汁中果汁含量测定方法探讨[J].农业科技与装备,2012,(7):76-78.Hu MK,Cen Q,Li D.Determination of juice content in juice[J].Agric Sci Technol Equip,2012,(7):76-78.
[5]张淼,李燮昕,贾洪锋,等.我国果汁掺假检测技术研究现状[J].食品研究与开发,2016,(5):205-207.Zhang M,Li XX,Jia HF,et al.The research on detection techniques of adulteration about fruit juice in China[J].Food Res Dev,2016,(5):205-207.
[6]王磊.柑桔汁中肌醇和可溶性糖的检测及含量特征分析[D].重庆:西南大学,2009.Wang L.Simultaneous determination of inositols and carbohydrates and content analysising in citrus juice[D].Chongqing:Southwest University,2009.
[7]孙达.不同柑橘种质资源中可溶性糖和有机酸含量的评价[D].武汉:华中农业大学,2013.Sun D.Soluble sugar and organic acid contents evaluation of different citrus germplasm resources[D].Wuhan:Hua Zhong Agricultural University,2013.
[8]GB/T 21731-2008橙汁及橙汁饮料[S].GB/T 21731-2008 Orange juice and orange juice beverages[S].
[9]CNAS-CL 01:2018检测和校准实验室能力认可准则[S].CNAS-CL 01:2018 Accreditation criteria for the competence of testing and calibration laboratories[S].
[10]中国实验室国家认可委员会.化学分析中不确定度的评估指南[M].北京:中国计量出版社,2002.China Laboratory National Accreditation Committee.Guidelines for the evaluation of uncertainty in chemical analysis[M].Beijing:China Metrology Publishing House,2002.
[11]顾鑫荣,张晓凡,董广彬,等.HPLC法测定蜂蜜中果糖,葡萄糖的不确定度评定[J].化学工程与装备,2008,(1):83-84.Gu XR,Zhang XF,Dong GB,et al.Uncertainty evaluation for the determination of fructose and glucose in honey by high performance liquid chromatography[J].Chem Eng Equip,2008,(1):83-84.
[12]GB/T 21730-2008浓缩橙汁[S].GB/T 21730-2008 Concentrated orange juice[S].
[13]JJF 1059.1-2012测量不确定度评定与表示[S].JJF 1059.1-2012 Measurement uncertainty evaluation and representation[S].
[14]CNAS-GL 06:2006化学分析中不确定度的评估指南[S].CNAS-GL 06:2006 Guidance on evaluating the uncertainty in chemical analysis[S].
[15]JJG 196-2006常用玻璃量器[S].JJG 196-2006 Common standards for measuring glass[S].
[16]国家技术监督局.测量不确定度的评定与表示指南[M].北京:中国计量出版社,2000.The state bureau of quality and technical supervision.Guidance on the evaluation and expression of measurement uncertainty[M].Beijing:China Metrology Publishing House,2000.
[2]USDA.Citrus:World markets and trade[EB/OL].[2018-5-16].http:www.fas.usda.gov/psdonline/circulars/citrus.pdf.
[3]曾祥国.不同种类和产区柑橘糖酸含量及组成研究[D].武汉:华中农业大学,2005.Zeng XG.Studies on contents and composition of sugar and acid in citrus from different species and production areas[D].Wuhan:Huazhong Agricultural University,2005.
[4]胡梦坤,岑琴,李丹.果汁中果汁含量测定方法探讨[J].农业科技与装备,2012,(7):76-78.Hu MK,Cen Q,Li D.Determination of juice content in juice[J].Agric Sci Technol Equip,2012,(7):76-78.
[5]张淼,李燮昕,贾洪锋,等.我国果汁掺假检测技术研究现状[J].食品研究与开发,2016,(5):205-207.Zhang M,Li XX,Jia HF,et al.The research on detection techniques of adulteration about fruit juice in China[J].Food Res Dev,2016,(5):205-207.
[6]王磊.柑桔汁中肌醇和可溶性糖的检测及含量特征分析[D].重庆:西南大学,2009.Wang L.Simultaneous determination of inositols and carbohydrates and content analysising in citrus juice[D].Chongqing:Southwest University,2009.
[7]孙达.不同柑橘种质资源中可溶性糖和有机酸含量的评价[D].武汉:华中农业大学,2013.Sun D.Soluble sugar and organic acid contents evaluation of different citrus germplasm resources[D].Wuhan:Hua Zhong Agricultural University,2013.
[8]GB/T 21731-2008橙汁及橙汁饮料[S].GB/T 21731-2008 Orange juice and orange juice beverages[S].
[9]CNAS-CL 01:2018检测和校准实验室能力认可准则[S].CNAS-CL 01:2018 Accreditation criteria for the competence of testing and calibration laboratories[S].
[10]中国实验室国家认可委员会.化学分析中不确定度的评估指南[M].北京:中国计量出版社,2002.China Laboratory National Accreditation Committee.Guidelines for the evaluation of uncertainty in chemical analysis[M].Beijing:China Metrology Publishing House,2002.
[11]顾鑫荣,张晓凡,董广彬,等.HPLC法测定蜂蜜中果糖,葡萄糖的不确定度评定[J].化学工程与装备,2008,(1):83-84.Gu XR,Zhang XF,Dong GB,et al.Uncertainty evaluation for the determination of fructose and glucose in honey by high performance liquid chromatography[J].Chem Eng Equip,2008,(1):83-84.
[12]GB/T 21730-2008浓缩橙汁[S].GB/T 21730-2008 Concentrated orange juice[S].
[13]JJF 1059.1-2012测量不确定度评定与表示[S].JJF 1059.1-2012 Measurement uncertainty evaluation and representation[S].
[14]CNAS-GL 06:2006化学分析中不确定度的评估指南[S].CNAS-GL 06:2006 Guidance on evaluating the uncertainty in chemical analysis[S].
[15]JJG 196-2006常用玻璃量器[S].JJG 196-2006 Common standards for measuring glass[S].
[16]国家技术监督局.测量不确定度的评定与表示指南[M].北京:中国计量出版社,2000.The state bureau of quality and technical supervision.Guidance on the evaluation and expression of measurement uncertainty[M].Beijing:China Metrology Publishing House,2000.