基于整合药理学平台研究葛根芩连汤治疗小儿肺炎的作用机制
|
摘要
目的基于整合药理学平台探索葛根芩连汤治疗小儿肺炎的作用机制。方法本研究通过中药整合药理学平台,通过输入葛根、黄芩、黄连、甘草四味药及肺炎获取药物和疾病靶标,然后进行蛋白质-蛋白质相互作用及网络构建,进一步进行基因功能和通路富集分析,进行葛根芩连汤治疗肺炎的"中药-成分-靶标-通路"多维网络分析。结果药物作用于疾病的关键通路包括细胞生长和死亡、传染病、细胞凋亡、免疫系统、趋化因子信号通路、B细胞受体信号通路、T细胞受体信号通路、血小板活化、胃酸分泌等。药物潜在靶标为GCK、COX7C、ATP1A1、ADCY2。结论葛根芩连汤治疗小儿肺炎的机制可能通过与能量代谢及消化系统相关物质唾液等相关,推测其具有潜在的关系。
Objective To explore the mechanism of Gegen Qinlian Decoction in the treatment of children with pneumonia,and to find out the path and target of Gegen Qinlian Decoction in the treatment of children with pneumonia.Methods Through the integrated pharmacology platform of traditional Chinese medicine,the drug and disease targets were obtained by inputting puerarin,jaundice,berberine,licorice and pneumonia,and then protein-protein interaction and network construction were carried out to further carry out gene function and pathway enrichment.analysis.Results Key pathways for drug action in disease including cell growth and death,infectious diseases,apoptosis,immune system,chemokine signaling pathway,B cell receptor signaling pathway,T cell receptor signaling pathway,platelet activation,gastric acid Secretion and so on.Potential drug targets are GCK,COX7C,ATP1A1,and ADCY2.Conclusion The mechanism of Gegen Qinlian Decoction in the treatment of children with pneumonia may be presumed to have a potential relationship through the relationship with energy metabolism and salivary system related substances
Objective To explore the mechanism of Gegen Qinlian Decoction in the treatment of children with pneumonia,and to find out the path and target of Gegen Qinlian Decoction in the treatment of children with pneumonia.Methods Through the integrated pharmacology platform of traditional Chinese medicine,the drug and disease targets were obtained by inputting puerarin,jaundice,berberine,licorice and pneumonia,and then protein-protein interaction and network construction were carried out to further carry out gene function and pathway enrichment.analysis.Results Key pathways for drug action in disease including cell growth and death,infectious diseases,apoptosis,immune system,chemokine signaling pathway,B cell receptor signaling pathway,T cell receptor signaling pathway,platelet activation,gastric acid Secretion and so on.Potential drug targets are GCK,COX7C,ATP1A1,and ADCY2.Conclusion The mechanism of Gegen Qinlian Decoction in the treatment of children with pneumonia may be presumed to have a potential relationship through the relationship with energy metabolism and salivary system related substances
引文
[1]汪受传,赵霞,韩新民,等.小儿肺炎喘嗽中医诊疗指南[J].中医儿科杂志,2008,4(3):1-3.
[2]孙丹,李新民,韩耀巍,等.从湿热论治小儿肺炎喘嗽[J].中医杂志,2017,58(22):1965-1967.
[3]邢心睿,吕狄亚,柴逸峰,等.网络药理学在中药作用机制中的研究进展[J].药学实践杂志,2018,36(2):97-102.
[4]王萍,唐仕欢,苏瑾,等.基于整合药理学的中药现代研究进展[J].中国中药杂志,2018,43(7):1297-1302.
[5]许海玉,杨洪军.整合药理学:中药现代研究新模式[J].中国中药杂志,2014,39(3):357-362.
[6]Yu G,Zhang Y,Ren W,et al.Network pharmacologybased identification of key pharmacological pathways of Yin-Huang-Qing-Fei capsule acting on chronic bronchitis[J].Int J Chron Obstruct Pulmon Dis,2017,12:85-94.
[7]许海玉,刘振明,付岩,等.中药整合药理学计算平台的开发与应用[J].中国中药杂志,2017,42(18):3633-3638.
[8]杨蒙蒙,孟静茹,王立锋,等.葛根芩连汤中总生物碱体外抗轮状病毒作用实验研究[J].中华实用诊断与治疗杂志,2016,30(10):968-971.
[9]郭友立.葛根芩连汤沉淀物溶出度及盐酸小檗碱、葛根素配伍肠吸收变化的研究[D].广州:南方医科大学,2010.
[10]邱爱珠,陈宝田.葛根芩连汤证为感染性腹泻病治的论证研究[J].热带医学杂志,2010,10(6):640-642.
[11]杜茜.以体外细胞模型探究葛根芩连汤剂颗粒消化道—淋巴—胰腺作用途径[D].福州大学,2014.
[12]任雅君,屈会化,赵琰,等.葛根芩连汤体内作用靶点的研究[A].中华中医药学会.全国第二十二次仲景学说学术年会论文集[C].内蒙古呼和浩特.2014.
[13]章常华,马广强,邓永兵,等.葛根芩连汤对KK-Ay糖尿病小鼠血浆中LPS、TNF-α、IL-6及肠道菌群的影响[J].中草药,2017,48(8):1611-1616.
[14]韩耀巍,李新民,杜洪喆.湿热证小儿肺炎喘嗽辨证施治1例[J].中医药信息,2013,30(5):83-84.
[15]黄国东,孙秀玉,陈书,等.网络药理学在国内中药复方研究的应用概况[J].广西中医药大学学报,2016,19(1):104-107.
[2]孙丹,李新民,韩耀巍,等.从湿热论治小儿肺炎喘嗽[J].中医杂志,2017,58(22):1965-1967.
[3]邢心睿,吕狄亚,柴逸峰,等.网络药理学在中药作用机制中的研究进展[J].药学实践杂志,2018,36(2):97-102.
[4]王萍,唐仕欢,苏瑾,等.基于整合药理学的中药现代研究进展[J].中国中药杂志,2018,43(7):1297-1302.
[5]许海玉,杨洪军.整合药理学:中药现代研究新模式[J].中国中药杂志,2014,39(3):357-362.
[6]Yu G,Zhang Y,Ren W,et al.Network pharmacologybased identification of key pharmacological pathways of Yin-Huang-Qing-Fei capsule acting on chronic bronchitis[J].Int J Chron Obstruct Pulmon Dis,2017,12:85-94.
[7]许海玉,刘振明,付岩,等.中药整合药理学计算平台的开发与应用[J].中国中药杂志,2017,42(18):3633-3638.
[8]杨蒙蒙,孟静茹,王立锋,等.葛根芩连汤中总生物碱体外抗轮状病毒作用实验研究[J].中华实用诊断与治疗杂志,2016,30(10):968-971.
[9]郭友立.葛根芩连汤沉淀物溶出度及盐酸小檗碱、葛根素配伍肠吸收变化的研究[D].广州:南方医科大学,2010.
[10]邱爱珠,陈宝田.葛根芩连汤证为感染性腹泻病治的论证研究[J].热带医学杂志,2010,10(6):640-642.
[11]杜茜.以体外细胞模型探究葛根芩连汤剂颗粒消化道—淋巴—胰腺作用途径[D].福州大学,2014.
[12]任雅君,屈会化,赵琰,等.葛根芩连汤体内作用靶点的研究[A].中华中医药学会.全国第二十二次仲景学说学术年会论文集[C].内蒙古呼和浩特.2014.
[13]章常华,马广强,邓永兵,等.葛根芩连汤对KK-Ay糖尿病小鼠血浆中LPS、TNF-α、IL-6及肠道菌群的影响[J].中草药,2017,48(8):1611-1616.
[14]韩耀巍,李新民,杜洪喆.湿热证小儿肺炎喘嗽辨证施治1例[J].中医药信息,2013,30(5):83-84.
[15]黄国东,孙秀玉,陈书,等.网络药理学在国内中药复方研究的应用概况[J].广西中医药大学学报,2016,19(1):104-107.