酪蛋白磷酸肽检测方法研究进展
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摘要
综述食品中酪蛋白磷酸肽的检测方法,主要包括高效液相色谱法、液相色谱-质谱联用法、毛细管区带电泳法、氯化钡-乙醇沉淀法等,分别介绍几种检测方法的优缺点及检测过程中面临困难。研究数据表明,高效液相色谱法具有操作简单、快速、灵敏度高等特点,在众多领域应用最为广泛。今后酪蛋白磷酸肽的检测方法应该以高效液相色谱法为基础,探索适用性更强的检测方法。为建立食品中统一的酪蛋白磷酸肽的国标检测方法提供有力参考。
This article reviewed the detection methods of casein phosphopeptides in food,including high performance liquid chromatography,high performance liquid chromatography-mass spectrometry,capillary zone elect rophoresis and Barium-ethanol precipitation,etc.The advantages and disadvantages of several detection methods and the difficulties in the detection process were introduced.The research data showed that high performance liquid chromatography had the characteristics of simple operation,rapidity and high sensitivity,and was widely used in many fields.It was considered that the detection method of casein phosphopeptide should be based on high performance liquid chromatography to explore a more applicable detection method.The present paper could provided a powerful reference for the establishment of a national standard detection method for the uniform casein phosphopeptides in food.
This article reviewed the detection methods of casein phosphopeptides in food,including high performance liquid chromatography,high performance liquid chromatography-mass spectrometry,capillary zone elect rophoresis and Barium-ethanol precipitation,etc.The advantages and disadvantages of several detection methods and the difficulties in the detection process were introduced.The research data showed that high performance liquid chromatography had the characteristics of simple operation,rapidity and high sensitivity,and was widely used in many fields.It was considered that the detection method of casein phosphopeptide should be based on high performance liquid chromatography to explore a more applicable detection method.The present paper could provided a powerful reference for the establishment of a national standard detection method for the uniform casein phosphopeptides in food.
引文
[1]孙延春.酪蛋白磷酸肽应用研究进展[J].中国乳品工业,2011,39(2):53-55
[2]Tuloglu N,Bayrak S,Tunc E S,et al.Effect of fluoride varnish with added casein phosphopeptide-amorphous calcium phosphate on the acid resistance of the primary enamel[J].Bmc Oral Health,2016,16(1):103
[3]Esenlik E,UzerE,Bolat E.Efficacy of a casein phosphopeptide amorphous calcium phosphate(CPP-ACP)paste in preventing white spot lesions in patients with fixed orthodontic appliances:Aprospective clinical trial[J].European Journal of Paediatric Dentistry,2016,17(4):274-280
[4]徐曼,何东平,卫娜,等.酪蛋白磷酸肽持钙能力的研究[J].现代食品科技,2012,28(3):278-281
[5]TULIPANO G,BULGARI O,CHESSA S,et al.Direct effects of casein phosphopeptides on growth and differentiation of in vitro cultured osteoblastic cells(MC3T3-E1)[J].Regulatory Peptides,2010,160(1):168-174
[6]PEREGO S,ZABEO A,MARASCO E,et al.Casein phosphopeptides modulate calcium uptake and apoptosis in Ca CO2cells through their interaction with the TRPV6 calcium channel[J].Journal of Functional Foods,2013,5(2):847-857
[7]WANG Xu,LI Ming,LI Min,et al.Zinc-bindingcapacity of yak casein hydrolysate and the zincreleasing characteristics of casein hydrolysate-zinc com plexes[J].Journal of Dairy Sceince,2011,94:2731-2740
[8]Ceci M,Mirando M,Beltrami R,et al.Protective effect of casein phosphopeptide‐amorphous calcium phosphate on enamel erosion:Atomic force microscopy studies[J].Scanning,2015,37(5):327-334
[9]MAYNE R J,COCHRANE N J,CAI F,et al.In-vitro study of the effect of casein phosphopeptide amorphous calcium fluoride phosphate on iatrogenic damage to enamel during orthodontic adhesive removal[J].American Journal of Orthodontics&Dentofacial Orthopedics,2011,139(6):543-551
[10]王玉莹,陈锡威,冯凤琴,等.酪蛋白磷酸肽的研究进展[J].食品工业,2014,35(5):204-208
[11]KARGUL B,ALTINOK B,WELBURY R.The effect of casein phosphopeptide-amorphous calcium phosphate on enamel surface rehardening.An in vitro study[J].European Journal of Paediatric Dentistry,2012,13(2):123-127
[12]左超.新型功能性食品[J].内蒙古石油化工,2010,36(14):23-29
[13]中华人民共和国卫生部.GB 14880-2012食品安全国家标准食品营养强化剂使用标准[S].北京:中国标准出版社,2012:10-18
[14]冯凤琴,陈闽军,杜永盛,等.高效液相色谱法测定奶粉中酪蛋白磷酸肽(CPP)含量[J].中国食品学报,2006(3):128-132
[15]焦宇知,王璋,许时婴.酪蛋白磷酸肽新工艺及其体外功能性质[J].食品与生物技术学报,2004(1):89-93
[16]中华人民共和国国家卫生和计划生育委员会.GB 31617-2014食品安全国家标准食品营养强化剂酪蛋白磷酸肽[S].北京:中国标准出版社,2014:3-4
[17]陈雪香,罗珍,刘飞,等.酪蛋白磷酸肽的质量评价方法研究[J].食品工业科技,2012,33(8):75-77,82
[18]张帅,刘飞,罗珍,等.高效液相色谱法测定钙片中酪蛋白磷酸肽的含量[J].现代食品科技,2014,30(7):258-262,285
[19]杨霞,冯锋,刘荔贞,等.高效毛细管区带电泳法同时检测3个产地沙棘果粉中的活性物质[J].食品科学,2017,38(14):144-150
[20]MARINA A D,JOHAN J,魡SA E,et al.Capillary electrophoresis method for the simultaneous determination of carbohydrates and proline in honey samples[J].Microchemical Journal,2016,129:1-4
[21]DELLA B F,VITALI L,FETT R,et al.Development and validation of a sub-minute capillary zone electrophoresis method for determination of nitrate and nitrite in baby foods[J].Talanta,2014,122:23-29
[22]ADAMSON N,RILEY P F,REYNOLDS E C.The analysis of multiple phosphoseryl-containing casein peptides using capillary zone electrophoresis[J].Journal of Chromatography A,1993,646(2):391-396
[23]王利民,郝艳红,王静,等.毛细管区带电泳测定饲料添加剂中酪蛋白磷酸肽含量的方法研究[J].黑龙江畜牧兽医,2001(3):1-2
[24]高启奇,黄思棋,肖光辉,等.高效液相色谱法测定乳粉中酪蛋白磷酸肽含量[J].食品科技,2016,41(7):292-296
[25]牟光庆,张丽萍,王立民,等.毛细管区带电泳测定酪蛋白磷酸肽方法的研究[J].中国食品学报,2002(2):21-24
[26]ZHANG Shijuan,SUN Y,SUN Z,et al.Determination of triterpenic acids in fruits by a novel high performance liquid chromatography method with high sensitivity and specificity[J].Food Chemistry,2014,146(1):264-269
[27]SABATINO L,SCARANGELLA M,LAZZARO F,et al.Matrine and oxymatrine in corroborant plant extracts and fertilizers:HPLC/MS-MS method development and single-laboratory validation[J].Journal of Environmental Science&Health.part.b Pesticides Food Contaminants&Agricultural Wastes,2015,50(12):862-870
[28]Pietras R,Szymanska K,Kondej M,et al.Development and validation of the HPLC method for varenicline determination in pharmaceutical preparation[J].Current Issues in Pharmacy&Medical Sciences,2017,30(3):142-146
[29]张瑞华,李芳,康怀彬,等.动物源性产品中盐酸克伦特罗检测方法的研究进展[J].食品研究与开发,2017,38(3):208-211
[30]赖奕坚,陈闽军,刘玉敏,等.LC-MS法测定钙片中酪蛋白磷酸肽含量[J].食品科学,2006(12):672-674
[31]胡蓓,张京顺,柯星,等.高效液相色谱-串联质谱法测定婴幼儿配方奶粉中酪蛋白磷酸肽含量[J].食品科学,2018,39(18):286-291
[32]伍晓红,邢磊,聂婉,等.克伦特罗的检测方法研究进展[J].食品研究与开发,2017,38(4):203-207
[33]BAGOUB A,MEDINA-RODRIGUEZ S,GOMEZ-ROMERO M,et al.Assessing the varietal origin of extra-virgin olive oil using liquid chromatography fingerprints of phenolic compound,data fusion and chemometrics[J].Food Chemistry,2017,215:245
[34]CHEN Weicheng,LAI Yisyuan,LU Kanhung,et al.Method development and validation for the high-performance liquid chromatography assay of gastrodin in water extracts from different sources of Gastrodia elata,Blume[J].Journal of Food&Drug Analysis,2015,23(4):803-810
[35]MIQUEL E,GOMEZ J A,ALEGRIA A,et al.Identification of casein phosphopeptides inβ-casein and commercial hydrolysed casein by mass spectrometry[J].Food Science and Technology International,2006,12(5):379-384
[36]KOSALKOVA K,GARCIA-ESTRADA C,BARREIRO C,et al.Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori.Proteomics studies reveal changes in the secretory pathway[J].Microbial Cell Factories,2012(11):1-15
[37]田随安,张向兵,李永利.HPLC法测定保健食品中酪蛋白磷酸肽[J].中国卫生检验杂志,2007(2):273-306
[38]刘玉娟.高效液相色谱法测定婴幼儿配方乳粉中酪蛋白磷酸肽的含量[J].乳业科学与技术,2016,39(4):26-28
[39]梁艳,石丹,王艳萍.高效液相色谱法检测乳制品的酪蛋白磷酸肽[J].中国乳品工业,2016,44(9):56-57,60
[40]庞广昌,朱文欣,陈庆森.酪蛋白磷酸肽(CPPs)测定方法的研究[J].食品科学,2001(5):49-52
[2]Tuloglu N,Bayrak S,Tunc E S,et al.Effect of fluoride varnish with added casein phosphopeptide-amorphous calcium phosphate on the acid resistance of the primary enamel[J].Bmc Oral Health,2016,16(1):103
[3]Esenlik E,UzerE,Bolat E.Efficacy of a casein phosphopeptide amorphous calcium phosphate(CPP-ACP)paste in preventing white spot lesions in patients with fixed orthodontic appliances:Aprospective clinical trial[J].European Journal of Paediatric Dentistry,2016,17(4):274-280
[4]徐曼,何东平,卫娜,等.酪蛋白磷酸肽持钙能力的研究[J].现代食品科技,2012,28(3):278-281
[5]TULIPANO G,BULGARI O,CHESSA S,et al.Direct effects of casein phosphopeptides on growth and differentiation of in vitro cultured osteoblastic cells(MC3T3-E1)[J].Regulatory Peptides,2010,160(1):168-174
[6]PEREGO S,ZABEO A,MARASCO E,et al.Casein phosphopeptides modulate calcium uptake and apoptosis in Ca CO2cells through their interaction with the TRPV6 calcium channel[J].Journal of Functional Foods,2013,5(2):847-857
[7]WANG Xu,LI Ming,LI Min,et al.Zinc-bindingcapacity of yak casein hydrolysate and the zincreleasing characteristics of casein hydrolysate-zinc com plexes[J].Journal of Dairy Sceince,2011,94:2731-2740
[8]Ceci M,Mirando M,Beltrami R,et al.Protective effect of casein phosphopeptide‐amorphous calcium phosphate on enamel erosion:Atomic force microscopy studies[J].Scanning,2015,37(5):327-334
[9]MAYNE R J,COCHRANE N J,CAI F,et al.In-vitro study of the effect of casein phosphopeptide amorphous calcium fluoride phosphate on iatrogenic damage to enamel during orthodontic adhesive removal[J].American Journal of Orthodontics&Dentofacial Orthopedics,2011,139(6):543-551
[10]王玉莹,陈锡威,冯凤琴,等.酪蛋白磷酸肽的研究进展[J].食品工业,2014,35(5):204-208
[11]KARGUL B,ALTINOK B,WELBURY R.The effect of casein phosphopeptide-amorphous calcium phosphate on enamel surface rehardening.An in vitro study[J].European Journal of Paediatric Dentistry,2012,13(2):123-127
[12]左超.新型功能性食品[J].内蒙古石油化工,2010,36(14):23-29
[13]中华人民共和国卫生部.GB 14880-2012食品安全国家标准食品营养强化剂使用标准[S].北京:中国标准出版社,2012:10-18
[14]冯凤琴,陈闽军,杜永盛,等.高效液相色谱法测定奶粉中酪蛋白磷酸肽(CPP)含量[J].中国食品学报,2006(3):128-132
[15]焦宇知,王璋,许时婴.酪蛋白磷酸肽新工艺及其体外功能性质[J].食品与生物技术学报,2004(1):89-93
[16]中华人民共和国国家卫生和计划生育委员会.GB 31617-2014食品安全国家标准食品营养强化剂酪蛋白磷酸肽[S].北京:中国标准出版社,2014:3-4
[17]陈雪香,罗珍,刘飞,等.酪蛋白磷酸肽的质量评价方法研究[J].食品工业科技,2012,33(8):75-77,82
[18]张帅,刘飞,罗珍,等.高效液相色谱法测定钙片中酪蛋白磷酸肽的含量[J].现代食品科技,2014,30(7):258-262,285
[19]杨霞,冯锋,刘荔贞,等.高效毛细管区带电泳法同时检测3个产地沙棘果粉中的活性物质[J].食品科学,2017,38(14):144-150
[20]MARINA A D,JOHAN J,魡SA E,et al.Capillary electrophoresis method for the simultaneous determination of carbohydrates and proline in honey samples[J].Microchemical Journal,2016,129:1-4
[21]DELLA B F,VITALI L,FETT R,et al.Development and validation of a sub-minute capillary zone electrophoresis method for determination of nitrate and nitrite in baby foods[J].Talanta,2014,122:23-29
[22]ADAMSON N,RILEY P F,REYNOLDS E C.The analysis of multiple phosphoseryl-containing casein peptides using capillary zone electrophoresis[J].Journal of Chromatography A,1993,646(2):391-396
[23]王利民,郝艳红,王静,等.毛细管区带电泳测定饲料添加剂中酪蛋白磷酸肽含量的方法研究[J].黑龙江畜牧兽医,2001(3):1-2
[24]高启奇,黄思棋,肖光辉,等.高效液相色谱法测定乳粉中酪蛋白磷酸肽含量[J].食品科技,2016,41(7):292-296
[25]牟光庆,张丽萍,王立民,等.毛细管区带电泳测定酪蛋白磷酸肽方法的研究[J].中国食品学报,2002(2):21-24
[26]ZHANG Shijuan,SUN Y,SUN Z,et al.Determination of triterpenic acids in fruits by a novel high performance liquid chromatography method with high sensitivity and specificity[J].Food Chemistry,2014,146(1):264-269
[27]SABATINO L,SCARANGELLA M,LAZZARO F,et al.Matrine and oxymatrine in corroborant plant extracts and fertilizers:HPLC/MS-MS method development and single-laboratory validation[J].Journal of Environmental Science&Health.part.b Pesticides Food Contaminants&Agricultural Wastes,2015,50(12):862-870
[28]Pietras R,Szymanska K,Kondej M,et al.Development and validation of the HPLC method for varenicline determination in pharmaceutical preparation[J].Current Issues in Pharmacy&Medical Sciences,2017,30(3):142-146
[29]张瑞华,李芳,康怀彬,等.动物源性产品中盐酸克伦特罗检测方法的研究进展[J].食品研究与开发,2017,38(3):208-211
[30]赖奕坚,陈闽军,刘玉敏,等.LC-MS法测定钙片中酪蛋白磷酸肽含量[J].食品科学,2006(12):672-674
[31]胡蓓,张京顺,柯星,等.高效液相色谱-串联质谱法测定婴幼儿配方奶粉中酪蛋白磷酸肽含量[J].食品科学,2018,39(18):286-291
[32]伍晓红,邢磊,聂婉,等.克伦特罗的检测方法研究进展[J].食品研究与开发,2017,38(4):203-207
[33]BAGOUB A,MEDINA-RODRIGUEZ S,GOMEZ-ROMERO M,et al.Assessing the varietal origin of extra-virgin olive oil using liquid chromatography fingerprints of phenolic compound,data fusion and chemometrics[J].Food Chemistry,2017,215:245
[34]CHEN Weicheng,LAI Yisyuan,LU Kanhung,et al.Method development and validation for the high-performance liquid chromatography assay of gastrodin in water extracts from different sources of Gastrodia elata,Blume[J].Journal of Food&Drug Analysis,2015,23(4):803-810
[35]MIQUEL E,GOMEZ J A,ALEGRIA A,et al.Identification of casein phosphopeptides inβ-casein and commercial hydrolysed casein by mass spectrometry[J].Food Science and Technology International,2006,12(5):379-384
[36]KOSALKOVA K,GARCIA-ESTRADA C,BARREIRO C,et al.Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori.Proteomics studies reveal changes in the secretory pathway[J].Microbial Cell Factories,2012(11):1-15
[37]田随安,张向兵,李永利.HPLC法测定保健食品中酪蛋白磷酸肽[J].中国卫生检验杂志,2007(2):273-306
[38]刘玉娟.高效液相色谱法测定婴幼儿配方乳粉中酪蛋白磷酸肽的含量[J].乳业科学与技术,2016,39(4):26-28
[39]梁艳,石丹,王艳萍.高效液相色谱法检测乳制品的酪蛋白磷酸肽[J].中国乳品工业,2016,44(9):56-57,60
[40]庞广昌,朱文欣,陈庆森.酪蛋白磷酸肽(CPPs)测定方法的研究[J].食品科学,2001(5):49-52