鼻咽癌患者肿瘤组织中VEGF、COX-2与PIK3CA表达量的相关性分析
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摘要
目的探讨鼻咽癌患者肿瘤组织中血管内皮生长因子(VEGF)、环氧合酶-2(COX-2)与磷脂酰肌醇-3-激酶催化亚基α(PIK3CA)表达量的相关性。方法选取鼻咽癌患者34例作为研究对象,所有患者治疗后均随访3年,采集所有研究对象肿瘤组织标本并检测其VEGF、COX-2与PIK3CA表达情况,分析三者间相互关系,并结合患者临床资料探究上述指标对鼻咽癌发生发展的影响及临床意义。结果鼻咽癌患者肿瘤组织中VEGF、COX-2与PIK3CA表达阳性率与患者性别、年龄均无显著相关。COX-2表达阳性率与患者是否发生淋巴转移有关(P <0. 05),与患者肿瘤临床分期无关(P> 0. 05); VEGF、PIK3CA表达阳性率与肿瘤临床分期及淋巴结转移均有关系(P <0. 05)。通过Pearson相关性分析鼻咽癌组织中的VEGF蛋白、COX-2蛋白表达量与PIK3CA蛋白表达量,发现前二者与后者明显存在正相关关系(γ=0. 808、γ=0. 643,P均<0. 05)。治疗后3年,鼻咽癌组织中VEGF、COX-2与PIK3CA表达为阳性患者的生存率明显低于阴性表达患者(P <0. 05)。结论 VEGF、COX-2与PIK3CA表达情况与鼻咽癌的发生发展密切相关,三者间或存在一定的联系,可用于临床预测患者术后较长时间内的生存情况。
Objective To analyze the expression of vascular endothelial growth factor( VEGF),cyclooxygenase-2( COX-2) and phosphatidylinositol 3-kinase catalytic subunit α( PIK3 CA) in the tumor tissues of patients with nasopharyngeal carcinoma Correlation of expression levels. Methods A total of 34 patients with nasopharyngeal carcinoma diagnosed in our hospital from January 2014 to December 2014 were enrolled in this study. All patients were followed up for three years after treatment. Tumor samples from all the subjects were collected and their expressions of VEGF,COX-2 and PIK3 CA expression,analysis of the correlation between the three,combined with clinical data to explore the above indicators in the occurrence of nasopharyngeal carcinoma and its clinical significance. Results The positive rates of VEGF,COX-2 and PIK3 CA in nasopharyngeal carcinoma patients were not significantly correlated with gender and age,but the positive rates of COX-2 were correlated with lymph node metastasis( P < 0. 05) The positive rates of VEGF and PIK3 CA were correlated with TNM staging and lymph node metastasis( P <0. 05). Pearson correlation analysis showed that VEGF protein,COX-2( γ = 0. 808,γ = 0. 643,P < 0. 05). After 3 years of treatment,the expression of VEGF and PIK3 CA in nasopharyngeal carcinoma tissues was significantly higher than that in the control group The survival rates of COX-2 and PIK3 CA positive patients were significantly lower than those of negative patients( P <0. 05). Conclusion The expression of VEGF,COX-2 and PIK3 CA are closely related to the occurrence and development of nasopharyngeal carcinoma. There is a certain relationship between the expression of VEGF,COX-2 and PIK3 CA,which may be used to predict the survival of patients after a long period of operation.
Objective To analyze the expression of vascular endothelial growth factor( VEGF),cyclooxygenase-2( COX-2) and phosphatidylinositol 3-kinase catalytic subunit α( PIK3 CA) in the tumor tissues of patients with nasopharyngeal carcinoma Correlation of expression levels. Methods A total of 34 patients with nasopharyngeal carcinoma diagnosed in our hospital from January 2014 to December 2014 were enrolled in this study. All patients were followed up for three years after treatment. Tumor samples from all the subjects were collected and their expressions of VEGF,COX-2 and PIK3 CA expression,analysis of the correlation between the three,combined with clinical data to explore the above indicators in the occurrence of nasopharyngeal carcinoma and its clinical significance. Results The positive rates of VEGF,COX-2 and PIK3 CA in nasopharyngeal carcinoma patients were not significantly correlated with gender and age,but the positive rates of COX-2 were correlated with lymph node metastasis( P < 0. 05) The positive rates of VEGF and PIK3 CA were correlated with TNM staging and lymph node metastasis( P <0. 05). Pearson correlation analysis showed that VEGF protein,COX-2( γ = 0. 808,γ = 0. 643,P < 0. 05). After 3 years of treatment,the expression of VEGF and PIK3 CA in nasopharyngeal carcinoma tissues was significantly higher than that in the control group The survival rates of COX-2 and PIK3 CA positive patients were significantly lower than those of negative patients( P <0. 05). Conclusion The expression of VEGF,COX-2 and PIK3 CA are closely related to the occurrence and development of nasopharyngeal carcinoma. There is a certain relationship between the expression of VEGF,COX-2 and PIK3 CA,which may be used to predict the survival of patients after a long period of operation.
引文
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[2]姚霞,张晶晶. PIK3CA过表达与鼻咽癌发生及预后的关系〔J〕.临床耳鼻咽喉头颈外科杂志,2017,31(9):687-690.
[3] Sun GG,Lu YF,Cheng YJ,et al. Absent expression of FLNA is correlated with poor prognosis of nasopharyngeal cancer〔J〕. Tumour Biol,2014,35(4):74-2967-74.
[4] Wang S,Li S,Xie D,et al. CD44 regulates epithelial-mesenchymal transition and metastasis in nasopharyngeal cancer cells〔J〕. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi,2013,27(5):4-250.
[5]杨姣,蒿艳蓉,冯国生,等. COX多因素分析和ROC曲线综合评价DNA修复基因对鼻咽癌的预后价值〔J〕.基础医学与临床,2014,34(10):1333-1338.
[6] Zhang JX,Cai MB,Wang XP,et al. Elevated DLL4 expression is correlated with VEGF and predicts poor prognosis of nasopharyngeal carcinoma〔J〕. Med Oncol,2013,30(1):390.
[7] Wong CH,Loong HH,Hui CW,et al. Preclinical evaluation of the PI3K-mTOR dual inhibitor PF-04691502 as a novel therapeutic drug in nasopharyngeal carcinoma〔J〕. Invest New Drugs,2013,31(6):408-1399.
[8]郭俊宇.异甘草素对人鼻咽癌CNE2细胞裸鼠移植瘤模型的放疗增敏作用〔J〕.河北医药,2016,38(24):3704-3707.
[9] Soares S,Craveiro R,Catarino R,et al. The role of nt590P21 gene polymorphism in the susceptibility to nasopharyngeal cancer〔J〕. Exp Oncol,2014,36(1):7-44.
[10] Ooft ML,Braunius WW,Heus P,et al. Prognostic significance of the EGFR pathway in nasopharyngeal carcinoma:a systematic review and meta-analysis〔J〕. Biomark Med,2015,9(10):997-1010.
[11] Van Keymeulen A,Lee MY,Ousset M,et al. Reactivation of multipotency by oncogenic PIK3CA induces breast tumour heterogeneity〔J〕. Nature,2015,525(7567):23-119.
[12]余涛,孙杨,戚乐,等. VEGF与MVD表达对鼻咽癌的临床意义〔J〕.健康研究,2014,34(1):26-28.
[13] Shi D,Xiao X,Tian Y,et al. Activating enhancer-binding protein-2αinduces cyclooxygenase-2 expression and promotes nasopharyngeal carcinoma growth〔J〕. Oncotarget,2015,6(7):21-5005.
[14] Zhu H,Zhu X. Relationship between cyclooxygenase-2 innasopharyngeal carcinoma and cervical lymph node metastasis〔J〕. Zhonghua Yi Xue Za Zhi,2014,94(18):12-1409.
[15]刘伟.鼻咽癌与VEGF研究进展〔J〕.中外医学研究,2015,13(26):162-164.
[16]雍军,李林格,李亮等.鼻息肉与鼻咽癌标本VEGF水平及微血管密度对比及其临床意义〔J〕.现代生物医学进展,2017,17(16):3068-3071.
[17] Phipps AI,Ahnen DJ,Cheng I,et al. PIK3CA somatic mutation status in relation to patient and tumor factors in racial/ethnic minorities with colorectal cancer〔J〕. Cancer Epidemiol Biomarkers Prev,2015,24(7):51-1046.
[18] Scheffler M,Bos M,Gardizi M,et al. PIK3CA mutations in non-small cell lung cancer(NSCLC):genetic heterogeneity,prognostic impact and incidence of prior malignancies〔J〕. Oncotarget,2015,6(2):26-1315.
[19] Ma BB,Lui VW,Hui CW,et al. Preclinical evaluation of the m TOR-PI3K inhibitor BEZ235 in nasopharyngeal cancer models〔J〕. Cancer Lett,2014,343(1):24-32.
[20] Yip WK,He PY,Abdullah MA,et al. Increased expression of phosphatidylinositol 3-Kinase p110αand gene amplification of PIK3CA in nasopharyngeal carcinoma〔J〕. Pathol Oncol Res,2016,22(2):9-413.
[2]姚霞,张晶晶. PIK3CA过表达与鼻咽癌发生及预后的关系〔J〕.临床耳鼻咽喉头颈外科杂志,2017,31(9):687-690.
[3] Sun GG,Lu YF,Cheng YJ,et al. Absent expression of FLNA is correlated with poor prognosis of nasopharyngeal cancer〔J〕. Tumour Biol,2014,35(4):74-2967-74.
[4] Wang S,Li S,Xie D,et al. CD44 regulates epithelial-mesenchymal transition and metastasis in nasopharyngeal cancer cells〔J〕. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi,2013,27(5):4-250.
[5]杨姣,蒿艳蓉,冯国生,等. COX多因素分析和ROC曲线综合评价DNA修复基因对鼻咽癌的预后价值〔J〕.基础医学与临床,2014,34(10):1333-1338.
[6] Zhang JX,Cai MB,Wang XP,et al. Elevated DLL4 expression is correlated with VEGF and predicts poor prognosis of nasopharyngeal carcinoma〔J〕. Med Oncol,2013,30(1):390.
[7] Wong CH,Loong HH,Hui CW,et al. Preclinical evaluation of the PI3K-mTOR dual inhibitor PF-04691502 as a novel therapeutic drug in nasopharyngeal carcinoma〔J〕. Invest New Drugs,2013,31(6):408-1399.
[8]郭俊宇.异甘草素对人鼻咽癌CNE2细胞裸鼠移植瘤模型的放疗增敏作用〔J〕.河北医药,2016,38(24):3704-3707.
[9] Soares S,Craveiro R,Catarino R,et al. The role of nt590P21 gene polymorphism in the susceptibility to nasopharyngeal cancer〔J〕. Exp Oncol,2014,36(1):7-44.
[10] Ooft ML,Braunius WW,Heus P,et al. Prognostic significance of the EGFR pathway in nasopharyngeal carcinoma:a systematic review and meta-analysis〔J〕. Biomark Med,2015,9(10):997-1010.
[11] Van Keymeulen A,Lee MY,Ousset M,et al. Reactivation of multipotency by oncogenic PIK3CA induces breast tumour heterogeneity〔J〕. Nature,2015,525(7567):23-119.
[12]余涛,孙杨,戚乐,等. VEGF与MVD表达对鼻咽癌的临床意义〔J〕.健康研究,2014,34(1):26-28.
[13] Shi D,Xiao X,Tian Y,et al. Activating enhancer-binding protein-2αinduces cyclooxygenase-2 expression and promotes nasopharyngeal carcinoma growth〔J〕. Oncotarget,2015,6(7):21-5005.
[14] Zhu H,Zhu X. Relationship between cyclooxygenase-2 innasopharyngeal carcinoma and cervical lymph node metastasis〔J〕. Zhonghua Yi Xue Za Zhi,2014,94(18):12-1409.
[15]刘伟.鼻咽癌与VEGF研究进展〔J〕.中外医学研究,2015,13(26):162-164.
[16]雍军,李林格,李亮等.鼻息肉与鼻咽癌标本VEGF水平及微血管密度对比及其临床意义〔J〕.现代生物医学进展,2017,17(16):3068-3071.
[17] Phipps AI,Ahnen DJ,Cheng I,et al. PIK3CA somatic mutation status in relation to patient and tumor factors in racial/ethnic minorities with colorectal cancer〔J〕. Cancer Epidemiol Biomarkers Prev,2015,24(7):51-1046.
[18] Scheffler M,Bos M,Gardizi M,et al. PIK3CA mutations in non-small cell lung cancer(NSCLC):genetic heterogeneity,prognostic impact and incidence of prior malignancies〔J〕. Oncotarget,2015,6(2):26-1315.
[19] Ma BB,Lui VW,Hui CW,et al. Preclinical evaluation of the m TOR-PI3K inhibitor BEZ235 in nasopharyngeal cancer models〔J〕. Cancer Lett,2014,343(1):24-32.
[20] Yip WK,He PY,Abdullah MA,et al. Increased expression of phosphatidylinositol 3-Kinase p110αand gene amplification of PIK3CA in nasopharyngeal carcinoma〔J〕. Pathol Oncol Res,2016,22(2):9-413.