浙江省三个斑鳜野生群体线粒体DNA细胞色素b基因序列的遗传变异
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摘要
对钱塘江上游江山、乌溪江段和瓯江上游云和段3个斑鳜(Siniperca scherzeri Steindachner)群体线粒体DNA细胞色素b(Cyt b)基因片段进行了扩增和测序,比较并分析了其遗传多样性和群体遗传分化。结果显示,在斑鳜Cytb基因965 bp的部分序列中,A+T含量(52.11%)略高于G+C含量(47.89%),77个个体中发现了16个多态性位点,8种单倍型。江山群体、乌溪江群体和云和群体的单倍型多样性分别为0.570±0.078、0.668±0.067、0.667±0.032,核苷酸多样性分别为0.001 8±0.000 3、0.001 9±0.000 2、0.004 3±0.000 6。江山群体与乌溪江群体间的Fst为-0.005 16(P>0.05),表明同为钱塘江上游的两群体没有明显分化;但云和群体与江山群体、乌溪江群体间Fst分别为0.191 23(P<0.01)和0.178 10(P<0.01),表明瓯江上游与钱塘江上游斑鳜群体存在显著的分化。
The mt DNA Cyt b gene of Siniperca scherzeri Steindachner from the upper reaches of Qiantang River( Jiangshan population and Wuxijiang population) and the upper reaches of Oujiang River( Yunhe population) were amplified and sequenced to assess the genetic diversity and genetic structure. The results showed that the average content of A + T( 52. 11%) was higher than that of G + C( 47. 89%) in the aligned 965 bp partial sequences. A total of 16 polymorphic sites were detected and 8 haplotypes were recovered out of 77 individuals. The haplotype diversity of Jiangshan,Wuxijiang and Yunhe populations were 0. 570 ± 0. 078,0. 668 ± 0. 067 and 0. 667 ± 0. 032,respectively,while the nucleotide diversity of the three populations were 0. 001 8 ± 0. 000 3、0. 001 9 ± 0. 000 2、0. 004 3 ± 0. 000 6,respectively. The Fst value between Jiangshan population and Wuxijiang population was low( Fst =-0. 005 16,P >0. 05),which indicated that the two upper reaches of Qiantang River populations did not have significant genetic differentiation. While statistically significant genetic differentiations were observed between Yunhe population and Jiangshan population( Fst = 0. 191 23,P < 0. 01),and between Yunhe population and Wuxijiang population( Fst =0. 178 10,P < 0. 01),which indicated significant genetic differentiations between the upper reaches of Qiantang River and the upper reaches of Oujiang River.
The mt DNA Cyt b gene of Siniperca scherzeri Steindachner from the upper reaches of Qiantang River( Jiangshan population and Wuxijiang population) and the upper reaches of Oujiang River( Yunhe population) were amplified and sequenced to assess the genetic diversity and genetic structure. The results showed that the average content of A + T( 52. 11%) was higher than that of G + C( 47. 89%) in the aligned 965 bp partial sequences. A total of 16 polymorphic sites were detected and 8 haplotypes were recovered out of 77 individuals. The haplotype diversity of Jiangshan,Wuxijiang and Yunhe populations were 0. 570 ± 0. 078,0. 668 ± 0. 067 and 0. 667 ± 0. 032,respectively,while the nucleotide diversity of the three populations were 0. 001 8 ± 0. 000 3、0. 001 9 ± 0. 000 2、0. 004 3 ± 0. 000 6,respectively. The Fst value between Jiangshan population and Wuxijiang population was low( Fst =-0. 005 16,P >0. 05),which indicated that the two upper reaches of Qiantang River populations did not have significant genetic differentiation. While statistically significant genetic differentiations were observed between Yunhe population and Jiangshan population( Fst = 0. 191 23,P < 0. 01),and between Yunhe population and Wuxijiang population( Fst =0. 178 10,P < 0. 01),which indicated significant genetic differentiations between the upper reaches of Qiantang River and the upper reaches of Oujiang River.
引文
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[2]李思忠.鳜亚科鱼类地理分布的研究[J].动物学杂志,1991,26(4):40-44.LI S Z.Geographic distribution of the Sinipercinae fishes[J].Chinese Journal of Zoology,1991,26(4):40-44.(in Chinese with English abstract)
[3]孔晓瑜,周才武.鳜亚科Sinipercinae鱼类的LDH同工酶的比较研究[J].青岛海洋大学学报,1992,22(1):103-109.KONG X Y,ZHOU C W.Comparative studies on LDH isozyme in Sinipercinae fishes of China[J].Journal of Ocean University of Qingdao,1992,22(1):103-109.(in Chinese with English abstract)
[4]王伟伟,赵金良,李思发.我国斑鳜六个群体mt DNA Cyt b序列的遗传变异[J].动物学研究,2006,27(6):589-593.WANG W W,ZHAO J L,LI S F.Genetic variation of the mitochondrial DNA Cyt b among six populations of Siniperca scherzeri in China[J].Zoological Research,2006,27(6):589-593.(in Chinese with English abstract)
[5]梁旭方.国内外鳜类研究及养殖概况[J].水产科技情报,1996,23(1):13-17.LIANG X F.Study on mandarin fish and its culture home and abroad[J].Fisheries Science&Technology Information,1996,23(1):13-17.(in Chinese with English abstract)
[6]曾可为,王青云,高爱银,等.斑鳜的生物学及繁殖生物学的研究[J].内陆水产,2005,30(2):21-23.ZENG K W,WANG Q Y,GAO A Y,et al.Biology and reproduction study on Siniperca scherzeri[J].Inland Fisheries,2005,30(2):21-23.(in Chinese with English abstract)
[7]凌继忠.斑鳜人工繁育技术总结[J].渔业致富指南,2004(16):33-34.LING J Z.Artificial breeding technology of Siniperca scherzeri[J].Fishery Guide to be Rich,2004(16):33-34.(in Chinese)
[8]郭新红,刘少军,刘巧,等.鱼类线粒体DNA研究新进展[J].遗传学报,2004,31(9):983-1000.GUO X H,LIU S J,LIU Q,et al.New progresses on mitochondrial DNA in fish[J].Acta Genetica Sinica,2004,31(9):983-1000.(in Chinese with English abstract)
[9]ROGERS A R,HARPENDING H.Population growth makes waves in the distribution of pairwise genetic differences[J].Molecular Biology and Evolution,1992,9(3):552-569.
[10]谢楠,冯晓宇,郭水荣,等.河川沙塘鳢线粒体细胞色素b(Cyt b)基因序列分析[J].现代农业科技,2010(19):288-290.XIE N,FENG X Y,GUO S R,et al.Sequences analysis of mitochondrial cytochrome b gene fragment of Odontobutis potamophila[J].Modern Agricultural Science and Technology,2010(19):288-290.(in Chinese with English abstract)
[11]武菲,王翠华,胡文革,等.基于细胞色素b基因河鲈养殖群体与野生群体遗传多样性分析[J].淡水渔业,2015,45(6):16-21.WU F,WANG C H,HU W G,et al.An analysis of the gene diversity based on the mitochondrial cytochrome b gene sequence of wild and cultivated populations of Perca Fluviatilis[J].Freshwater Fisheries,2015,45(6):16-21.(in Chinese with English abstract)
[12]张艳萍,王太,杜岩岩,等.秦岭细鳞鲑人工繁育群体与野生群体遗传变异分析[J].水生生物学报,2014,38(5):828-833.ZHANG Y P,WANG T,DU Y Y,et al.Analysis of the genetic diversity of cultured and wild Brachymystax Lenok Tsinlingensis populations based on mt DNA D-loop and Cyt b[J].Acta Hydrobiologica Sinica,2014,38(5):828-833.(in Chinese with English abstract)
[13]GRANT W S,BOWEN B W.Shallow population histories in deep evolutionary lineages of marine fishes:insights from sardines and anchovies and lessons for conservation[J].Heredity,1998,89(5):415-426.
[14]AVISE J C.Phylogeography[M].Cambrige,Massachusetts:Harvard University Press,2000.
[15]WRIGHT S.The interpretation of population structure by FStatistics with special regard to systems of mating[J].Evolution,1965,19(3):395-420.