锦州某奶牛养殖场乳房炎大肠埃希菌药敏试验及耐药基因检测
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摘要
为了解锦州某奶牛养殖场临床型乳房炎病原菌耐药情况。对该场进行患病乳样采集、主要病原菌分离鉴定、药物敏感性试验和耐药基因筛选。结果表明,该场乳房炎主要病原菌为大肠埃希菌,共得到乳房炎大肠埃希菌分离株36株;药物敏感性试验结果表明,分离株对磺胺类药物高度耐药、对头孢噻呋和头孢唑啉敏感;对分离株进行磺胺类药物耐药基因筛查结果表明,sul1、sul2、sul3基因的阳性率分别为76.0%、17.1%和0%,说明sul1基因为该场大肠埃希菌磺胺类药物主要耐药基因。
To investigate the drug resistance situation of pathogenic bacteria in clinical mastitis of dairy breeding farm in Jinzhou,the milk were sampled and the pathogenic bacteria were isolated,and drug sensitivity tests were carried out,and resistance genes were screened.The results showed that the main pathogen was E.coli,36 strains were obtained.And drug sensitivity test showed that isolates were highly resistant to sulfanilamide and sensitive to ceftiofur and cefazolin.The isolation rate of sul1,sul2 and sul3 resistance genes were 76.0% ,17.1% and 0% respectively,suggesting that sul1 gene was the main resistance gene of sulfonamides in E.coli isolates.
To investigate the drug resistance situation of pathogenic bacteria in clinical mastitis of dairy breeding farm in Jinzhou,the milk were sampled and the pathogenic bacteria were isolated,and drug sensitivity tests were carried out,and resistance genes were screened.The results showed that the main pathogen was E.coli,36 strains were obtained.And drug sensitivity test showed that isolates were highly resistant to sulfanilamide and sensitive to ceftiofur and cefazolin.The isolation rate of sul1,sul2 and sul3 resistance genes were 76.0% ,17.1% and 0% respectively,suggesting that sul1 gene was the main resistance gene of sulfonamides in E.coli isolates.
引文
[1]王礼伟,施研进,屈勇刚,等.新疆石河子地区奶牛乳房炎大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2014,41(1):203-206.
[2]张金宝,马春芳,余婷,等.宁夏地区奶牛乳房炎大肠杆菌毒理基因检测和耐药性分析[J].畜牧兽医学报,2016,47(1):177-182.
[3]姚伟,于学武,曹东,等.辽宁地区奶牛乳房炎细菌性病原分离鉴定及大肠杆菌药敏试验[J].中国畜牧兽医,2016,43(11):3080-3084.
[4]项开合,张乃生,肖连明,等.奶牛乳房炎的生物学防治[J].动物医学进展,2007,28(3):109-112.
[5]张宇,刘磊,豆艳丽.奶牛乳房炎葡萄球菌的分离鉴定及耐药性分析[J].甘肃农业大学学报,2013,48(6):34-39.
[6]倪春霞,蒲万霞,胡永浩,等.奶牛乳房炎病原菌的分离鉴定及耐药性分析[J].西北农业学报,2010,19(2):20-24.
[7]韩笑,刀筱芳,张焕容,等.奶牛隐性乳房炎主要病原菌分离鉴定及耐药性分析[J].动物医学进展,2015,36(7):131-134.
[8]丁丹丹,张震,邓立新,等.河南省奶牛隐性乳房炎主要病原菌分离鉴定与药敏试验[J].中国奶牛,2015(10):23-27.
[9]刘耀川,张泽辉,王庄,等.辽宁阜新地区奶牛隐性乳房炎调查及大肠埃希菌药敏试验[J].动物医学进展,2015,36(11):133-136.
[10]刘开成,郑增忍,单虎,等.不同地区猪源大肠埃希菌磺胺类药物耐药性研究[J].动物医学进展,2011,32(4):76-79.
[11]陈绍辉,林雁春,曹海涛,等.吉林省部分地区猪源大肠杆菌磺胺耐药基因sul2的分布研究[J].江西畜牧兽医杂志,2008,16(6):8-10.
[12]庄林林.三重LAMP检测禽源沙门氏菌、大肠杆菌磺胺耐药基因(sul1、sul2、sul3)研究[D].江苏:扬州大学,2016.
[13]王娜,杨晓洪,郭欣妍,等.磺胺类耐药菌种抗性基因sul1的表达规律[J].生态毒理学报,2015,10(5):75-81.
[14]张倩,刘功成,屈勇刚,等.石河子地区奶牛乳房炎大肠埃希菌的分离鉴定及耐药性分析[J].动物医学进展,2016,37(7):115-119.
[15]赵晓丽,胡大春,杨辉,等.产ESBLs大肠埃希菌磺胺耐药基因sul I的检测与分析[J].昆明医学院学报,2011(12):138-139.
[16]吕文发,姜厚强,于录.临床分离大肠杆菌磺胺敏感性及磺胺抗性相关基因的检测[J].吉林农业大学学报,2010,32(3):340-344.
[17]韦庆兰,张济培,谭华龙,等.广东地区水禽源大肠杆菌耐药性和磺胺类耐药基因检测[J].中国家禽,2015,37(3):54-56.
[2]张金宝,马春芳,余婷,等.宁夏地区奶牛乳房炎大肠杆菌毒理基因检测和耐药性分析[J].畜牧兽医学报,2016,47(1):177-182.
[3]姚伟,于学武,曹东,等.辽宁地区奶牛乳房炎细菌性病原分离鉴定及大肠杆菌药敏试验[J].中国畜牧兽医,2016,43(11):3080-3084.
[4]项开合,张乃生,肖连明,等.奶牛乳房炎的生物学防治[J].动物医学进展,2007,28(3):109-112.
[5]张宇,刘磊,豆艳丽.奶牛乳房炎葡萄球菌的分离鉴定及耐药性分析[J].甘肃农业大学学报,2013,48(6):34-39.
[6]倪春霞,蒲万霞,胡永浩,等.奶牛乳房炎病原菌的分离鉴定及耐药性分析[J].西北农业学报,2010,19(2):20-24.
[7]韩笑,刀筱芳,张焕容,等.奶牛隐性乳房炎主要病原菌分离鉴定及耐药性分析[J].动物医学进展,2015,36(7):131-134.
[8]丁丹丹,张震,邓立新,等.河南省奶牛隐性乳房炎主要病原菌分离鉴定与药敏试验[J].中国奶牛,2015(10):23-27.
[9]刘耀川,张泽辉,王庄,等.辽宁阜新地区奶牛隐性乳房炎调查及大肠埃希菌药敏试验[J].动物医学进展,2015,36(11):133-136.
[10]刘开成,郑增忍,单虎,等.不同地区猪源大肠埃希菌磺胺类药物耐药性研究[J].动物医学进展,2011,32(4):76-79.
[11]陈绍辉,林雁春,曹海涛,等.吉林省部分地区猪源大肠杆菌磺胺耐药基因sul2的分布研究[J].江西畜牧兽医杂志,2008,16(6):8-10.
[12]庄林林.三重LAMP检测禽源沙门氏菌、大肠杆菌磺胺耐药基因(sul1、sul2、sul3)研究[D].江苏:扬州大学,2016.
[13]王娜,杨晓洪,郭欣妍,等.磺胺类耐药菌种抗性基因sul1的表达规律[J].生态毒理学报,2015,10(5):75-81.
[14]张倩,刘功成,屈勇刚,等.石河子地区奶牛乳房炎大肠埃希菌的分离鉴定及耐药性分析[J].动物医学进展,2016,37(7):115-119.
[15]赵晓丽,胡大春,杨辉,等.产ESBLs大肠埃希菌磺胺耐药基因sul I的检测与分析[J].昆明医学院学报,2011(12):138-139.
[16]吕文发,姜厚强,于录.临床分离大肠杆菌磺胺敏感性及磺胺抗性相关基因的检测[J].吉林农业大学学报,2010,32(3):340-344.
[17]韦庆兰,张济培,谭华龙,等.广东地区水禽源大肠杆菌耐药性和磺胺类耐药基因检测[J].中国家禽,2015,37(3):54-56.