西藏自治区鼠疫自然疫源地鼠疫耶尔森菌耐药及耐消毒剂基因的研究
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摘要
目的调查西藏自治区鼠疫自然疫源地是否存在耐药及耐消毒剂的鼠疫耶尔森菌(鼠疫菌)菌株,为鼠疫的临床治疗提供准确信息。方法根据美国国立生物技术信息中心公布的耐氨基糖苷类链霉素StrA和StrB基因,耐β-内酰胺类抗菌药物TEM、SHV和CTX-M基因,耐磺胺类药物Sul1、Sul2和Sul3基因序列,耐消毒剂耐药QacEdeltalsul1基因,分别在每个基因上设计1对引物,逐一对分离自西藏自治区鼠疫自然疫源地的鼠疫菌DNA进行PCR检测。结果阴性对照和阳性对照成立,355株鼠疫菌的PCR检测结果均为阴性,未发现耐链霉素、耐磺胺类药物及耐β-内酰胺类抗菌药物和耐消毒剂菌株。结论西藏自治区鼠疫自然疫源地鼠疫菌尚未出现耐药及耐消毒剂菌株。
Objective To understand whether there is a drug-or disinfectant-resistant strains in natural plague foci inTibet, and provide the accurate information for clinical treatment of plague. Methods According to the aminoglycosideresistant gene of streptomycin resistant, StrB, StrA, beta lactam antibiotics TEM, SHV, and CTX-M gene, sulfamilamideresistant Sul1, Sul2, and Sul3, and anti-disinfectant QacEdeltal-sul1 gene sequence the National Center for BiotechnologyInformation(NCBI)released,a pair of primers in each gene was designed separately. DNA of strains isolated from naturalplague foci in Tibet were amplified by PCR using every pair of primers. Results Negative and positive control wereestablished, samples by PCR amplification results were negative, there were no streptomycin, sulfamilamide and betalactam antimicrobial drug resistance genes and anti-disinfectant genes in strains studied. Conclusion The Tibetautonomous region of natural plague foci did not appear to have drug-or disinfectant-resistant Yersina pestis.
Objective To understand whether there is a drug-or disinfectant-resistant strains in natural plague foci inTibet, and provide the accurate information for clinical treatment of plague. Methods According to the aminoglycosideresistant gene of streptomycin resistant, StrB, StrA, beta lactam antibiotics TEM, SHV, and CTX-M gene, sulfamilamideresistant Sul1, Sul2, and Sul3, and anti-disinfectant QacEdeltal-sul1 gene sequence the National Center for BiotechnologyInformation(NCBI)released,a pair of primers in each gene was designed separately. DNA of strains isolated from naturalplague foci in Tibet were amplified by PCR using every pair of primers. Results Negative and positive control wereestablished, samples by PCR amplification results were negative, there were no streptomycin, sulfamilamide and betalactam antimicrobial drug resistance genes and anti-disinfectant genes in strains studied. Conclusion The Tibetautonomous region of natural plague foci did not appear to have drug-or disinfectant-resistant Yersina pestis.
引文
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[4]赵宏群,占堆,阮水良,等.西藏自治区2009-2011年鼠疫耶尔森菌分离株的生化特征[J].中国媒介生物学及控制杂志,2017,28(2):175-176,187.
[5]杨晓艳,辛有全,魏柏青,等.西藏藏南地区新增疫源县鼠疫菌病原学分析及流行病学意义[J].中华地方病学杂志,2015,34(4):247-249.DOI:10.3760/cma.j.issn.2095-4255.2015.04.005.
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[7]西绕若登,李景中,洛桑群增,等.西藏鼠疫流行现状与青藏铁路沿线鼠疫预防控制对策[J].中国地方病防治杂志,2008,23(3):198-200.
[8]王国钧,李敏,郑谊,等.环丙沙星治疗家兔实验感染鼠疫疗效观察[J].中国地方病学杂志,2005,24(5):564-565.
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[10]Galimand M,Guiyoule A,Gerbaud G,et al.Multidrug resistance in Yersinia pestis mediated by a transferable plasmid[J].N Engl J Med,1997,337(10):677-681.
[11]Guiyoule A,Gerbaud G,Buchrieser C,et al.Transferable plasmid-mediated resistance to streptomycin in a clinical isolate of Yersinia pestis[J].Emerg Infect Dis,2001,7(1):43-48.
[12]Chanteau S,Ratsitorahina M,Rahalison L,et al.Current epidemiology of human plague in Madagascar[J].Microbes Infect,2000,2(1):25-31.
[13]Dennis DT,Hughes JM.Multidrug resistance in plague[J].N Engl J Med,1997,337(10):702-704.
[14]Welch TJ,Fricke WF,Mc Dermott PF,et al.Multiple antimicrobial resistance in plague:an emerging public health risk[J].PLo S One,2007,2(3):e309.DOI:10.1371/journal.pone.0000309.
[15]Hinnebusch BJ,Rosso ML,Schwan TG,et al.High-frequency conjugative transfer of antibiotic resistance genes to Yersinia pestis in the flea midgut[J].Mol Microbiol,2002,46(2):349-354.
[16]Wagner DM,Runberg J,Vogler AJ,et al.No Resistance plasmid in Yersinia pestis,North America[J].Emerg Infect Dis,2010,16(5):885-887.DOI:10.3201/eid1605.090892.
[2]Carraro N,Matteau D,Luo P,et al.The master activator of inc A/C conjugative plasmids stimulates genomic islands and multidrug resistance dissemination[J].PLo S Genet,2014,10(10):e1004714.
[3]Li YJ,Dai EH,Cui YJ,et al.Different region analysis for genotyping Yersinia pestis isolates from China[J].PLo S One,2008,3(5):e2166.DOI:10.1371/journal.pone.0002166.
[4]赵宏群,占堆,阮水良,等.西藏自治区2009-2011年鼠疫耶尔森菌分离株的生化特征[J].中国媒介生物学及控制杂志,2017,28(2):175-176,187.
[5]杨晓艳,辛有全,魏柏青,等.西藏藏南地区新增疫源县鼠疫菌病原学分析及流行病学意义[J].中华地方病学杂志,2015,34(4):247-249.DOI:10.3760/cma.j.issn.2095-4255.2015.04.005.
[6]麻占军,蒋志勇.西藏自治区1966-2012年人间鼠疫流行病学分析[J].中国地方病防治杂志,2013,28(2):119-122.
[7]西绕若登,李景中,洛桑群增,等.西藏鼠疫流行现状与青藏铁路沿线鼠疫预防控制对策[J].中国地方病防治杂志,2008,23(3):198-200.
[8]王国钧,李敏,郑谊,等.环丙沙星治疗家兔实验感染鼠疫疗效观察[J].中国地方病学杂志,2005,24(5):564-565.
[9]卫生部应急办公室.鼠疫防控应急手册[M].北京:北京大学医学出版社,2009:142.
[10]Galimand M,Guiyoule A,Gerbaud G,et al.Multidrug resistance in Yersinia pestis mediated by a transferable plasmid[J].N Engl J Med,1997,337(10):677-681.
[11]Guiyoule A,Gerbaud G,Buchrieser C,et al.Transferable plasmid-mediated resistance to streptomycin in a clinical isolate of Yersinia pestis[J].Emerg Infect Dis,2001,7(1):43-48.
[12]Chanteau S,Ratsitorahina M,Rahalison L,et al.Current epidemiology of human plague in Madagascar[J].Microbes Infect,2000,2(1):25-31.
[13]Dennis DT,Hughes JM.Multidrug resistance in plague[J].N Engl J Med,1997,337(10):702-704.
[14]Welch TJ,Fricke WF,Mc Dermott PF,et al.Multiple antimicrobial resistance in plague:an emerging public health risk[J].PLo S One,2007,2(3):e309.DOI:10.1371/journal.pone.0000309.
[15]Hinnebusch BJ,Rosso ML,Schwan TG,et al.High-frequency conjugative transfer of antibiotic resistance genes to Yersinia pestis in the flea midgut[J].Mol Microbiol,2002,46(2):349-354.
[16]Wagner DM,Runberg J,Vogler AJ,et al.No Resistance plasmid in Yersinia pestis,North America[J].Emerg Infect Dis,2010,16(5):885-887.DOI:10.3201/eid1605.090892.