摘要
多功能化的集诊断与治疗于一体的纳米材料的研究受到人们越来越广泛的关注。本研究中,我们成功合成了RGD靶向环肽修饰的第三代树状大分子稳定的金纳米星,后与VEGF siRNAs共孵育形成复合物(RGD-Au DSNSs/siRNA)。该纳米材料可以有效地携带VEGF siRNA进入人脑胶质瘤癌细胞(U87MG细胞),并防止其被核糖核酸酶或血清内容物降解,且由于金纳米星和靶向环肽RGD的存在,使得其在U87MG细胞和移植肿瘤模型中特异性的C成像成为可能。值得一提的是,U87MG细胞与RGD-Au DSNSs/siRNA复合物共培养后经近红外激光照射,细胞存活率仅为25%,治疗效果明显优于单独的光热治疗和基因治疗效果。实验结果表明,该纳米材料有望作为一种多功能化的诊疗剂,同时实现CT成像,光热治疗和基因治疗的协同治疗,达到肿瘤诊疗的目的。
The development of versatile nanomaterials that combine diagnostic and therapeutic functions within a single nanoplatform is extremely important in both fundamental and clinical medical sciences. In this work, we report the synthesis of cyclic RGD-modified amine-terminated generation 3.0 poly(amidomine) dendrimer-stablized gold nanostars that can be effectively used for siR NA delivery. With the existence of RGD peptide and Au nanostars, the platform can be used for specific computed tomography(CT) imaging of U87 MG cells(a human glioma cell line) and the xenografted tumor model. In vitro experiments show that the viability of cells was only 25% after incubation with RGD-Au DSNSs/siR NA under NIR irradiation, which is much lower than single photothermal therapy or gene therapy. These results indicate that the multifunctional nanoconstruct is a promising platform for tumor CT imaging and combined photothermal therapy and gene therapy.
引文
[1]Kong,L.;Alves,C.S.;Hou,W.;Qiu,J.;Mo?hwald,H.;Toma?s,H.;Shi,X.ACS Appl.Mater.Interfaces.2015,7(8),4833-4843.
[2]Li,J.;Hu,Y.;Yang,J.;Wei,P.;Sun,W.;Shen,M.;Zhang,G.;Shi,X.Biomaterials.2015,38:10-21.