摘要
本文以‘秦光 2 号’油桃为试材，研究了 MA 冷藏条件下，油桃果实采后呼吸速
率、乙烯生成、结构物质降解、软化相关酶类活性等的变化以及对果实品质因子的影
响，探讨了 MA 冷藏对油桃果实成熟软化的控制及作用机制，为 MA 冷藏在果实保
鲜上的应用提供理论依据与技术参考。
试验结果表明：（1）油桃是典型的跃变型果实，具有明显的呼吸跃变高峰和乙烯
释放高峰。MA 冷藏可显著抑制油桃果实贮藏期间的乙烯释放量，并使其跃变出现时
间延迟较对照 5d，同时推迟呼吸跃变的产生，抑制呼吸速率的上升。
（2）MA 冷藏对油桃果实失重率及果实硬度下降的抑制效果显著，使其硬度开
始下降的时期及失重率迅速上升的时期均较对照推迟 10d。
（3）MA 冷藏一定程度地抑制了油桃果实细胞壁类物质粗纤维、原果胶的降解
及其相关酶类 PG、CX 活性的上升，因此使果实的成熟软化进程趋缓，延长了贮藏
保鲜期至 60d。同时还延迟了膜相对透性的升高。
（4）MA 冷藏有利于油桃果实品质的保持，在不同程度上延缓了果实 Vc，可滴
定酸，可溶性蛋白质的变化进程及叶绿素的降解，推迟了果实色泽转变及失水皱缩的
发生，但对采后果实可溶性固形物的降解无明显抑制作用。
The effects of MA cold storage on respiration rate, the rate of ethylene production, the
variation of cellwall substance (cellulosepectin), the activities of amylase correlated with
softening (PG、CX) and the qualities of “Qinguang2hao”nectarine fruits during ripening
and softening were studied in this thesis. The functionary mechanism of MA cold storage
inhbiting nectarines ripening and softening were also discussed, which provided theoretical
bases and technical reference for MA storage applying to fruits storage.
The results of study showed that (1)‘Qinguang2hao’nectarine is the typical
climacteric fruit, it had great respiration peak and ethylene peak. MA cold storage could
significant reduce the ethylene production, and delayed the appearing time of ethylene
peak for 5days.It postphoned the appearing time of respiration peak and inhibited the
ascent of respiration rate,too.
(2) There were remarkable effects on fruit firmness and weight loss. MA cold storage
retarded the begainning time of firmress and weight loss for 10d.
(3) To some extent, MA cold storage restrained the hydrolysis of cellulose and pectin
and the increase of the amylase activities relating to fruit softening like PG and CX , so the
ripening and softening of the nectarine fruits was retarded to 60d, as well as lowed the
relative membrane permeability.
(4) MA cold storage made for keeping the nectarine fruits qualities, it prolonged the
variation process in Vc, titratable acidity, water-soluble protein, chlorophyl content and
colour changes. But there was no remarkable effect on the changes of TSS content.
It indicated that MA cold storage could delayed the nectarine fruits ripening and
softening ,so extend the post-harvest shelf life.
引文
1. 蔡同一. 我国果品产业化发展与食品面临的新问题[J]. 粮油加工与食品机械，2001，7：7-8
2. 闫瑞香，王仁才. 果实软化衰老的生理生化机制[J]. 湖南农业大学学报，2000，26（3）：230-235
3. 杨德兴，戴京晶 ，庞向宇等. 猕猴桃衰老过程中 PG、果胶质和细胞壁超微结构的变化[J]. 园艺
学报，1993，20（4）：341-345
4. 梁小娥，赵迎丽等. 枣采后果肉软化的生化和细胞超微结构的变化[J]. 园艺学报，1998，25（4）：
333-337
5. 罗自生. MA 贮藏对桑果细胞壁组分和水解酶活性的影响[J]. 果树学报，2003，20（3）：214-217
6. 方建雄，华雪增，刘愚. 贮藏温度和气体状况对苹果果胶、多聚半乳糖醛酸酶变化的影响[J]. 植
物生理学报，1991，17（1）：99-104
7. 关军峰，马智宏. 苹果果实软化与果胶含量、质膜透性和钙溶性的关系[J]. 果树学报，2001，18
（1）：11-14
8. 吕昌文，齐灵，修德仁等. 桃波动温度贮藏及其机理研究[J]. 华北农学报，1994，9（1）：75-80
9. 王俊宁. 1-MCP 处理对油桃果实软化衰老的影响[学位论文]. 陕西杨凌：西北农林科技大学，2002
10. 林河通，陈绍军等. 黄花梨果实采后软化生理基础[J]. 中国农业科学，2003，36（3）：349-352
11. 周培根，罗祖友，戚晓玉等. 桃成熟期间果实软化与果胶及有关酶的关系[J]. 南京农业大学学报，
1991，14（2）：33-37
12. 王贵禧，韩雅珊，于梁. 浸钙对猕猴桃果实硬度变化影响的生化机制[J]. 园艺学报，1995，22（1）：
21-24
13. 饶景萍，任小林. 园艺产品贮运学. 西安：陕西人民出版社，2003
14. 夏春森，王兰英. 红星苹果在贮藏中果肉发绵生理过程的研究[J]. 园艺学报，1981，3（2）：29-36
15. 薛严斌，高乔绫等. 番茄果实采后硬度变化的理化解析[J]. 保鲜与加工，2002，6：19-20
16. 田建文，许明宪，贺普超. 柿果实采收后软化生理分析[J]. 植物生理学通讯，1991，27（2）：109-111
17. 陆春贵，徐鹤林，周立新. PG、ACO、乙烯对番茄果实成熟的影响[J]. 园艺学报，1995，22（1）：
57-60
18. 生吉萍，申琳，罗云波. 果实成熟衰老相关酶研究进展[J]. 食品与机械，2000，77（3）：7-9
19. 周宏伟，吴耕西.长把梨贮藏中 PG 和 PE 的作用[J]. 山东农业大学学报，1992，23（1）：67-70
20. 彭丽桃，饶景萍等. 采后两种不同果肉类型油桃软化相关酶活性的变化[J]. 热带亚热带植物学
报，2002，10（2）：171-176
21. 童斌. 柿成熟衰老中果肉超微结构的变化及其与生理代谢的关系[学位论文]. 陕西杨凌：西北农
业大学，1999
22. 陆定志，傅家瑞，宋培泉. 植物衰老及其调控. 北京：中国农业出版社，1997
23. 王贵禧，韩雅珊，于梁. 猕猴桃软化过程中阶段专一性酶活性变化的研究[J]. 植物学报，1995，
37（3）：198-203
24. 陈昆松，李方，张上隆. 猕猴桃果实成熟进程中木葡聚糖内糖基转移酶 mRNA 水平的变化[J]. 植
物学报，1999，41（11）：1231-1234
25. 王贵喜，韩雅珊，于梁. 猕猴桃总淀粉酶活性与果实软化的关系[J]. 园艺学报，1994，21（4）：
329-333
26. 苍晶，王学东等. 狗枣猕猴桃果实软化过程中阶段性专一酶的研究[J]. 果树学报，2001，18（5）：
284-287
34 MA 冷藏对油桃果实成熟软化的控制
27. 徐昌杰，陈昆松，张上隆. 蔗糖酶对外切纤维素酶和外切半乳糖醛酸酶活性测定的干扰及排除[J].
植物生理学通讯，1997，33（1）：43-46
28. 陈昆松，张上隆. 采后果实成熟衰老的分子生物学机理及其调控. 植物分子生理学进展， 浙江
大学出版社，2000
29. 黄森，张继澍. 低乙烯减压处理对柿果实乙烯生物合成的影响[J]. 西北植物学报，2001，21（2）：
318-322
30. 朱东兴，饶景萍，李省印. 果蔬保鲜剂应用研究概述. 陕西农业科学，2003，（1）30-33
31. 钱永华，柿子采后主要生理生化变化及 AOA 处理对其影响[学位论文]. 陕西杨凌：西北农业大
学基础科学系，1993
32. 刘愚. 乙烯生物合成及其调节研究进展[J]. 植物生理学通讯，1986，（1）：60-66
33. 吴有梅，华雪增，方建雄等. 贮藏温度和气体组成对苹果乙烯生物合成的影响[J]. 植物生理学报，
1991，17（2）：169-176
34. 金勇丰，张耀洲. 桃果实 ACC 合成酶 cDNA 克隆[J]. 园艺学报，2000，27（4）：257-262
35. 李正国. 果实成熟的基因调控. 生物工程进展，2000，20（3）：30-34
36. 韦军，田边贤二. 温度对采后梨果实多胺、ACC 含量、EFE 活性和乙烯生成量的影响[J]. 园艺学
报，1994，21（2）：139-144
37. 李嘉瑞，周燕飞. 低压条件下“拳杏”果实的乙烯代谢[J]. 西北农业大学学报，1993，21（2）：
13-18
38. 关军锋，束怀瑞，黄天栋. 钙对新红星苹果乙烯生成的作用[J]. 园艺学报，1991，18（3）：205-209
39. 任小林，金志强，彭世清等. 猕猴桃 ACC 氧化酶 cDNA 克隆及全序列测定[J]. 园艺学报，1997，
24（4）：333-337
40. 金勇丰，张耀洲. 桃果实 ACC 合成酶基因的克隆和植物表达载体的构建[J]. 园艺学报，1998，
25（1）：37-43
41. 丁晓东. 荔枝分子生物学研究. 福建农业大学，1999，21-44
42. 丁长奎. 果实成熟过程中激素调控[J]. 植物生理学通讯，1990，5：5-9
43. 叶志彪，李汉霞，郑田琏等. 反义 ACC 氧化酶基因的导入对乙烯生成的抑制作用[J]. 华中农业
大学学报，1996，15（4）：305-309
44. 宋俊奇. 通过表达 ACC 脱氨酶基因控制番茄果实的成熟[J]. 生物工程学报，1998，14（1）：33-37
45. 王阳光，席芳等. 气调和乙烯对采后青梅果实.保绿效果及生理的影响[J]. 食品科学，2002，23
（90）：102-105
46. 田世平，徐勇，姜爱丽等. 冬雪蜜桃在气调冷藏期间品质及相关酶活的性的变化[J]. 中国农业科
学，34（6）：656-651
47. 王世宽，潘明. 塑料薄膜小包装贮藏江安李可行性研究[J]. 四川轻工学院学报，2001，14（2）：
59-61
48. 王愈，陈丽. 不同贮藏条件对山楂果胶酶活性及果胶含量影响的研究[J]. 山西果树，1999，2：
3-4
49. 屈红霞，唐友林，谭兴杰等. 低温贮藏对菠萝细胞壁降解的影响[J]. 园艺学报，2000，27（1）：
23-26
50. 窦世娟，陈昆松，吕均良等. 黄花梨采后不同处理的贮藏效果及其生理基础的研究[J]. 中国农业
科学，2003，36（1）：82-88
参考文献 35
51. 张培正，李坤. 采收成熟度、温度、气体成分对青州蜜桃保鲜效果的影响[J]. 食品工业科技，2002，
23（5）：58-60
52. 刘勤. 贮藏条件对枇杷贮藏期主要品质和生理变化的影响[J]. 南京农业大学学报，1994，17（1）：
27-31
53. 毕阳，高渊。张傲德. 黄瓜聚乙烯薄膜单果包装常温贮藏的研究[J]. 食品科学. 1994，11，69-71
54. 李素芬，郑素月. MA 贮藏对芫荽贮藏性的影响[J]. 农业与技术，2000，20（5）：23-24
55. 李素芬，郑素月. MA 贮藏对西兰花贮藏性的影响[J]. 农业与技术，2000，20（5）：20-22
56. 关文强，陈丽. 芜菁贮藏性及 MA 贮藏保鲜技术[J]. 天津农业科学，2003，9（2）：25-27
57. 辛广，侯冬岩. 薄膜包装和低温贮藏对百合鲜花保鲜效果的影响[J]. 沈阳农业大学学报，1999，
30（4）：426-429
58. 冯晓元，刘玲. 顶香油桃采后生理及贮藏技术研究[J]. 北方果树，2000（4）：25-27
59. 吴敏，陈昆松，张上隆. 桃果实采后成熟过程中脂氧合酶活性的变化[J]. 园艺学报，1999，26（4）：
227-231
60. 杨书珍，任小林等. GA 处理对采后油桃果实膜脂过氧化的影响[J]. 西北植物学报，2001，21（3）：
575-578
61. 皮玉珍，马青松，王善广等. 桃采后及贮藏生理研究进展[J]. 果树科学，2001，18（1）：53-56
62. 王贵禧，宋亦飞，梁丽松. 桃综合保鲜技术研究[J]. 林业科学研究， 1998，11（1）：30-33
63. 王俊宁，饶景萍. 1-MCP 处理对油桃果实硬度、呼吸及乙烯合成的影响[J]. 西北植物学报，2002，
22（5）：1171-1175
64. 高慧，饶景萍. 不同冷藏温度对油桃采后生理的影响[J]. 果树学报，2004，21（2）：173-175
65. 刘坤，李兴超. 油桃冷藏试验[J]. 北方果树，2000，16：16
66. 韩雅珊主编. 食品化学实验指导. 中国农业大学出版社，1996
67. 高俊凤主编. 植物生理学实验技术. 世界图书出版公司，2000
68. 刘兴华主编. 农产品贮藏运销学实验指导. 1997
69. 孟雪雁，岑涛. 桃低温贮藏中生理变化与冷害发生的关系[J]. 山西农业大学学报,2001，21（3）：
269-270
70. 李钰，盛其潮，张素梅. 蔬菜贮藏生理及气调技术[M]. 上海：上海科学技术出版社，1984，49-50
71. 张红艳. 不同贮藏温度对湘南梨果实品质和乙烯释放的影响[J]. 湖北农业科学，2003，5：67-69
72. 汪沂，田世平等. 不同冷藏温度对北京 33 号桃采后生理的影响[J]. 江西农业大学学报。2000，
22：491-493
73. 方建雄，华雪增，刘愚. 贮藏温度和气体状况对苹果果胶、多聚半乳糖醛酸酶变化的影响[J]. 植
物生理学报，1991，17（1）：99-104
74. 茅林春，张上隆. 果胶酶和纤维素酶在逃果实成熟和败絮中的作用[J]. 园艺学报，2001，28（2）：
107-111
75. 不同冷藏温度下桃冷藏效果及冷害症状的发生[J]. 山西农业大学学报，2001，21（1）：66-69
76. 张维一. 果蔬采后生理学[M]. 北京：农业出版社，1993
77. 茅林春，应铁进，张上隆. 桃果实败絮与果胶物质变化和细胞壁结构的关系[J]. 植物生理学报，
1999，25：121-126
78. 屈红霞，唐友林，谭兴杰等. 采后菠萝贮藏品质与果肉细胞壁结构的关系[J]. 果树学报，2001，
18（3）：164-167
36 MA 冷藏对油桃果实成熟软化的控制
79. 陆胜民，张耀洲. 梅果采后软化与细胞壁组分及其降解酶活性的变化[J]. 中国农业科学，2003，
36（5）：595-598
80. 颜季琼，张孝琪，龙程. 高等植物细胞壁的结构和功能的分子生物学基础[M]. 科技出版社，1992
81. 唐友林，周玉婵，潘小平. 采后芒果的后熟软化与贮藏淀粉变化之间的关系[J]. 亚热带植物通讯，
1997， 26（2）：40-44
82. 周国忠，刁太清. 猕猴桃果实淀粉含量和淀粉酶变化与贮藏性的关系[J]. 果树学报，1997，14（1）：
21-23
83. 张子德，马俊莲，宗汝静. 薄膜包装对黄瓜采后保鲜因子的研究[J]. 河北农业大学，1996，19（1）：
40-43
84. 郑永华. 枇杷薄膜包装贮藏效果研究[J]. 食品科学，2000，21（8）：56-58
85. 侯建设，李中华. 菠菜的薄膜包装冷藏效果研究[J]. 食品科技，2003，5：94-96.
86. 华中农业大学. 蔬菜贮藏加工学[M]. 北京：农业出版社，1997
87. 王文生，周山涛. 京白梨冷藏和气调贮藏的研究[J]. 制冷学报，1997，5：25-29
88. 高峻平，郭康等. 月季切花聚乙烯包装运输保鲜技术初探[J]. 园艺学报，1995，22（1）：77-82
89. 饶景萍，任小林. 果实成熟过程中组织超微结构的变化[J]. 西北植物学报，1997，17（1）：128-134
90. 童斌，饶景萍等. 柿子成熟过程中几种生理代谢及细胞膜透性的变化[J]. 西北植物学报，1999，
19（3）：482-486
91. 朱东兴，饶景萍等. 柿果实 1-MCP 处理对成熟软化的影响[J]. 园艺学报，2004，31（1）：87-89
92. 安克，鞠志国等. 多聚半乳糖醛酸酶与果实后熟研究进展[J]. 莱阳农学院学报，1994，11（2）：
113-117
93. 饶景萍，朱东兴. 1-MCP 对柿贮藏期间几种生理变化的影响[J]. 西北植物学报，2004，24（6）：
1061-1065
94. 彭丽桃，饶景萍等. 油桃后熟衰老期间几个生理指标的变化[J]. 西北农业学报，2001，10（4）：
37-40
95. 杨书珍，饶景萍. GA 对油桃保鲜效果研究[J]. 西北园艺，2002，5：7-8
96. Dawson DW, Melton LD,Watkin CB,et al. Cell wall changes in nectarines solubilization and
depolymerization of pectic and neutral polymers during ripening and in mealy fruit[J]. Plant
Physiol,1992, 100: 1203-1210
97. Ben AR, Kisler N. Ultrustructural changes in cell wall of appele and pear fruits[J]. Plant Physiol, 1972,
64: 197-202
98. Yamaki S. Changes in cell wall polysaccharides and monosaccharides during development an ripening
of Japanese pear fruits[J]. Plant Cell Physiol, 1979, 20(2): 311-321
99. Fils-lycaon BM, Buret. Loss of firmness and changes in Pectin factions during ripening and over
ripening of sweet cherry[J]. Hortscience, 1990, 25(7): 777-778
100. Ning B, Kubo y, et al. Softening characteristics of Chinese pear ‘yali’fruit with special relation to
changes in cell wall Polysacchariches and their degrading enzymes. Scientific Reports of the Faculty of
Agriculture Okayama University, 1997, 86: 71-78
101. Damson DM, Walkins CB, Melton LD. Intermittent harming affects cell wall composition of
“Fantasia” nectarines during ripening and storage[J]. Jour Amer Soc Hort Sci, 1995, 120(6):
1057~1062
102. Koch JL, Nevins DJ. The tomato fruit cell wall Ⅱ. Polyuronide metabolisim in non-softening tomato
参考文献 37
mutant[J]. Plant physiol, 1990, 92(3): 642~647
103. Tsayz M. Studied on the physio-chemical properties of posr-harvest sugar apple[J]. Acta Hortcultural,
1990, 269: 241-247
104. Ben-Arie R, Kisler N, Frenbel C. Degradation and Solubilization of Pectin by β-galactosidases
Purified from avocado mesocarp[J]. Plant Physiol,1993, 87: 279~285
105. Buescher RW, Sistrunk WA, Tigohelar EL,et al. Revalation of the changes in polygalacturonases in
tomatoes during ripening. Planta, 1988, 174: 39-43
106. Smith CJS, Watson C F, Ray J, et al. Antisense RNA inhibition of Polygalacturonse gene expression in
transgenic tomato[J]. Nature, 1988, 334: 724-727
107. Wegrzyn TF, MacRae EA. Pectinesterase, Polygalacturonase, and β-galactosidase during softening
of ethylene-treated kiwifruit[J]. Hortscience, 1992, 27(8): 900-902
108. Redgwell R J,et al. Cell wall dissolution in ripening kiwifruit solubilization of the pectic polymers[J].
Plant Physiol, 1992, 98: 71
109. Gaffe J, Tiznadi ME, Handa AK. Characterization and functional expression of a ubiquitously
expressed tomato pectin methyl esterase. Plant Physiol, 1997,114(4):1547~1556
110. Abeles FB, Takeda T. Cellulase activity and ethylene in ripening strawberry and apple fruits. Sci
Hortic, 1990,42:269~275
111. Christoftersen RE, Cass LG, McGarvey DJ, et al. Characterization and expression of a
ripening-induced cellulase gene from avocado. In:Osborne D J and Jackson M B.(eds), cell separation
in Plant. Physiology, Biochemistry and molecular Biology, 21~30, Berlin:springer-verag, 1989
112. Barnes MF, Patchett BJ. Cell wall degraded enzymes and the softening of senescent strawberry fruit
[J]. Food Sci, 1976, 41: 1392-1395
113. Huber DJ. Strawberry fruit softening: the potential roles of polyurondies and bemicelluloses[J]. Food
Sci, 1984, 49: 1310-1315
114. Redgwell RJ, Fry SC. Xyloglucan endotransglycosylase activity increases during kiwifruit(Actinidia
deliciosa) ripening[J]. Plant Physiol, 1993, 103: 1399-1406
115. Schroder R, Atkinson RG, Langenkmper G, et al. Biochemical and molecular Characterization of
xyloglucan endotrans glycosylase from ripe kiwifruit[J]. Planta, 1998, 204(2): 242-251
116. Wills RHB. Enhance of senescence in non-climacteric fruit and vegetables by low ethylene levels[J].
Acta Hortcultural, 1998, 464: 159-1622
117. Rottman MW, Peter GF, Oeller PW, et al. 1-aminocyclopropane-carboxylate syathase in tomato
encoded by a mulgene family whose transcipton is induced during fruit and floral senescence[J]. J Mol
Biol, 1991, 222: 937
118. Ross GS, Knighton ML, Lang-yee M. An ethylene-related cDNA from ripening apples[J]. Plant Mol
Biol, 1992, 19: 213-238
119. Peck SC, Olson DC, Kende HA. A cDNA sequence encoding 1-aminocyclopropane-carboxylate from
pea[J]. Plant Physiol, 1993, 101: 689-690
120. Wang H, Woodson WR. A flower senescence-related mRNA from carnation shares sequence similarity
with fruit-related mRNAs involved in ethylene biosynthesis[J]. Plant Physiol, 1991, 90: 1000-1001
121. Mcgarvey DJ, Yu H, Christofferson RE, et al. Nucleotide sequence of a ripening –related cDNA from
avocado fruit[J]. Plant Mol Biol, 1990, 15: 165
122. Guo L N, Artela R N, Pnillips A T,et al. Purification and Characterization of 1-aminolyclopropape-
1-Carboxylate N-malonyltransferase from elidated mung bean hypocotys[J]. Plant Physiol, 1992, 100:
2041
38 MA 冷藏对油桃果实成熟软化的控制
123. Ecker JR. The ethylene signal transduction pathway in plants[J]. Science, 1995,268: 667-675
124. Bleeker AB, Kenyon WH, Sommerville SC,et al. Use of monoclonal antibodies in the purification and
characterization of 1-aminocyclopropene-1-carboxylate synthase, an enzyme in ethylene biosythesis[J].
Pro Natl Acad Sci USA, 1986, 83:7755
125. Bleecker A B, Schaller G. The mechanism of ethylene perception[J]. Plant Physiol, 1996, 111: 653-660
126. Schaller G E, Bleecker A B. Ethylene-binding sites generated in yeast expressing the Arabidopsis
ETR1 gene[J]. Science, 1995, 270:1809-1811
127. Hua J, Chang C, Sun Q, et al. Ethylene-insensitivity conferred by Arabidopsis ERS gene[J]. Science,
1995, 269: 1712-1714
128. Sisler E C, Serek M. Compounds controlling the ethylene receptor[J]. Bot Bull Acad sin,1999, 40: 1-7
129. Oller PW, Min-Wong L, Taylor LP,et al. Reversible inhibition of tomato during senescence by antisene
RNA[J].Science, 1991, 254: 437-439
130. Hamilton AJ, Lycett GW,Grierson D. Antisene gene that inhibits synthesis of the hormone ethylene in
transgenic plants[J]. Nature, 1990, 346:284
131. Klee HJ, Hayford MB, Kretzmer KA,et al. Control of ethylene synthesis by expression of a bacterial
enzyme in transgenic tomato plants[J]. Plant cell. 1991(3):1187-1193
132. Kramer MG, Kellogy J, Wagoner W, et al. Reduced ethylene synthesis and ripening control in tomatoes
expressing sadenosylmethionine hydrolase[J]. NATO ASI ser,1997, 34: 307-319
133. Mathews H, Cohen W, Wayorer C, et al. Genetic transformation of raspberry with a gene to control
ethylene of biosynthesis[J]. Hort Sci,1994, 29:454
134. L.van den Berg.et.al. High humidity storage of vegetables and fruits[J]. Hortscience, 1978, (13):
565-569
135. Ben-Yehosha.S. Individual seal-packaging of fruits and vegetables in plastic film-A new postharvest
technique[J]. Hortscience, 1986, (20): 32-37
136. Prusky D, Prez A, Zutkhi Y, et,al. Effect of modified atmosphere for control of black spot, caused by
Alternaria alternata, on stored persimmon fruits[J]. Phytopathology, 1997, 87 (2): 203-208
137. Tian S P,Folchi A,Pratelig GC,et.al. The correlations of some physiological propecties during ultra low
oxygen storage in nectarines[J].Acta Hort, 1996, 374: 131-140
138. Wreight K P,Kader A A, Effect of controlled-atmosphere storage on the quality and carctenoid content
of sliced persimmons and peaches[J]. Postharvest Biol.Technic, 1997, 10: 89-97
139. Tian S P,Fan Q,Xu Y,et.al. Evaluation the use of high CO2 concentrations and cold storage to control
of Monilimia fructicola on sweet cherries[J].Postharvest Biol.Technic, 2001, 21(1): 53-60
140. Moura M A, Lopes L C, Moretti C L. Postharvest quality of an astringent persimmon variety after
long-term storage followed by ethephon treatment[J]. Proceedings of Florida State Horticultural
Society, 1996, 109: 289-290
141. Remon S. Used of modified atmosphere to prolong the postharvest life of Borlat cherry at two
different degree of ripeness[J]. J Sci Food Agric, 2000, 80: 1545-1552
142. Singh UP. Artifcial ripening of loquat (E.japonica Lindl). Sci and Cult,1959, 24: 335-337
143. N.Aharoni, A.Reuveni, O.Dvir. Modified atmospheres in film packages delay senescence and decay of
fresh herbs[J]. Acta Horticulturae, 1989, 258: 255-262
144. Sarah E. Lingle, Gene E. Lester, James R. Dunlap. Effect of postharvest heat treatment and storage on
sugar metabolism in polyethylene-wrapped muskmelon fruit[J]. Hortscience, 1987, 22(5): 917-919
145. Charles F. Forney, Roger E. Rij, Stanley R. Ross. Measurement of broccoli respiration rate in
film-wrapped packages[J]. Hortscience, 1989, 24(1): 111-113
参考文献 39
146. Victor R, Batia H, Yakov V, et al. Modified-atmosphere packaging improves keeping quality of
charentais-type melons[J]. Hortscience, 2002, 37(6): 950-953
147. George D. High-temperature conditioning to delau internal breakdown development in peaches and
nectarines[J]. Hort. Sci, 1991, 26(7): 882-885
148. Artes F. Quality attributes, pectolytic enzyme activities and physiological changes during postharvest
ripening of nectarines[J]. Journal of Food Quality.,1996, 19(6): 491-503
149. Eksteen,G.J. 1984. A summary of recent research on wooliness in l ocakky grown nectarines[J].
Deciduous Fruit Grower, 34: 389-392
150. Von Mollendorff,L.J. 1987. Woolliness in peaches and nectarines: A review. 1. Maturity and external
factors. Hort. Sci, 5: 1-3
151. Von Mollendorff,L.J, Jacob G,Devilliers T. Effects of temperature and fruit size on firmness,
extractable juice, wollinessand browning in two nectarine cultivars. Journal of Hortscience, 1992,
67(5): 647-654
152. Zhi Guo Ju. Leatheriness and mealiness of peaches in relation to fruit maturity and storage
tempeature[J]. Jounal of Horticalture Science & Biotechnology, 2000, 75(1): 86-91
153. Anderson RE, Penny RW. Intermittent warming of peaches and nectarines stored in a controlled
atmosphere or air. [J]. Amer Soc. Hort Sci., 1975, 100: 151-153
154. Anderson RE. Long-Term storage of peaches and nectarines during coldrooled atmosphere storage. [J].
Amer Soc. Hort Sci, 1982, 107: 214-216
155. Kader A. Postharvest decay , respiration, ethylene production and quality of each helb in controlled
atmosphere with added carbon monoxide[J]. Amer Soc. Hort Sci, 1982, 107: 856-859
156. Anlerson PE, Smilanic JL. Quality of nectarines stored in insecticidal low-O2 atmosphere at 5℃ and
15℃[J]. Amer Soc. Hort Sci., 1989, 114: 431-436
157. X.fan. Interactive effects of 1-MCP and temperature on ‘Elberta’peach quality[J]. Hort. Sci, 2002,
37(1): 134-138
158. NERYA. O. Pre and postharvest treatments of nectarines for prevention of postharvest decay[J].
J.Alon Hanotea, 1998, 52(7): 299-303
159. Dong L, Zhou HW, Feng X, et al. The role of ethylene in development of storage disorders in
nectarine and plum[J]. Acta Hortscience, 2001, 553(1): 285-288
160. Munoz Z, Veronica PZ. Effects of the maturity storage and prior storage temperature on the quality
of nectarines. Santiago（Chile）1992, 71
161. Smilanick J,Retamaies J,Cooper T,et.al. Preventing physiological disorders in nectarines by CA-and
high-CO2-storage[J].Gartenbauwssenschaft, 1992, 57: 166-172
162. Boughi C. Peach ripening: the invovement of cellase and polygalacturonase. Proceeding of the Third
Congress of European Society for Agronomy. Italy September, 1994, 580-581
163. Hruschka HW, Wang CY. Storage and shelf life of packaged watercress, parsley and mint. USDA,
Mktg. Res. Rep, 1979, 1102
164. Jurin V, M. Karel. Studies on control of respiration of McIntosh apples by packaging methods[J]. Food
Technol, 1963, 17: 782-786
165. Shewfelt H L, Meyers S C, Prussia S E, et al. Quality of fresh-market peaches within the postharvest
handling system[J]. Journal of Food Science, 1987, 52: 361-364
166. Lipton WJ. Controlled atmosphere for fresh vegetables and fryits-why and when. Postharvest Biology
and Handling of Fruits and Vegetables. AVI PubI. CO.,Westport, CT. PP. 1975, 130-143
167. Bonghi C, Raminu A, Ruperti B. Peach fruit ripening and quality to relation in picking time, and
40 MA 冷藏对油桃果实成熟软化的控制
hypoxie and high CO short-team postharvest treatments[J]. Postharvest Biol.Technic, 1999,
16:213-222
168. Fischer RL, Bennett AB. Role of cell wall hydrolases in fruit ripening[J]. Annual Review Plant
Physiology Plant Molecule Biology, 1991, 42: 675-703
169. Zhang JY, Liao XJ. Relativity between changes of firmness and pectin during plum picking storage[J].
Food Science, 1995, 22(3): 292-300
170. Kende H. Plant Physiol. 1989, 91: 1-4
171. Wang CY. Modified atmosphere packaging alleviates chilling injury in cucumbers[J]. Postharvest
Biology and Technology, 1997, 10: 195-200
172. Pesis Edna. Modified atmosphere and modified humidity packaging alleviates chilling injury in mango
fruit[J]. Postharvest Biology and Technology, 2000, 19: 93-101
173. Fallik E, Temkin-Gorodeiski N, et al. Prolonged low-temperature storage of eggplants in polyethylene
bags[J]. Postharvest Biology Technology, 1995, 5: 83-89
174. Silvia del C Rodriguez, Beatriz Lopez, et al. Effect of different treatment on the evolution of
polyamines during rifrige storage eggplants[J]. Agric Food, 2001, 49: 4700-4705
175. Mitcham EJ, McDonald Re. Cell wall modification during ripening of ‘Kitt’ and ‘Tommy Aktins’
mongo fruit[J]. Amer Sco Hort Sci, 1992, 117(6): 919-924
176. Yoshiok H, Aoda K, Kashimura Y. Molecular weight and degree of methoxylation in cell wall
polyurionide during softening in apple and pear fruit[J]. Amer Sco Hort Sci, 1992, 117(4): 600-600
177. DM Hinton, R Pressey. The activities of cell wall hydrolase in the process of peach softening[J]. Amer
Sco Hort Sci, 1980, 115(4): 499-502
178. Picton S, Bouzayen M, et al. Altered fruit ripening and leaf senescence in tomatoes expressing an
antisense ethylene-forming enzyme transgene[J]. Plant J, 1993, 3: 469
179. Tong B, Rao JP. Ultrastural changes in the mesocarp cell in relation to the physiological metabolism of
persimmon fruit during ripening and senescence[J]. Acta Horticulture, 2003, 601:199-203
180. Tonutti P, Bonghi C,et al. Biochamical and molecular aspects of peach fruit ripening[J].
181. Ali ZA, Brady CJ. Purification and characterization of the polygalacturonases of tomatoe fruits[J].
Aust J Plant Physiol, 1982, 9: 155-169
182. Neelam P, sanwal GG. Multiple forms of polygalacturonase from banana fruits[J]. Phytochmistry,
1998,4 8(2): 249-255
183. Giovannoni JJ, Dellapenna D, et al. Expression of a chimeric polygalacturonase gene in transgentic rin
tomato fruit results in polyuronide degradation but not softening[J]. Plant Cell, 1989, 1: 53-56
184. CrooksPR, Grierson D. Plant Physiol, 1983, 72: 1088-1093
185. Hilary C, Colin B. Purification of three pectinesterase from ripe peach fruit[J]. Phytochemistry, 1994,
37: 949-955
186. Cameron RG, Grohmann K. Partial purification and thermal characterization of pectinmethy- lesterase
from red grape fruit finisher pulp[J]. Journal of Food Science, 1995, 60(4): 821- 825
187. Cameron RG, Grohmann k. Purification and characterization of a thermally tolerant pectin
methylesterase from a commercial valencia fresh frozen orange juice[J]. Journal of Agricultural and
Food Chemistry, 1996, 446(2): 458-462
188. Gaffe J, Tiznadi ME, Handa AK. Characterization and functional expression of a ubiquitously
expressed tomato pectin methylesterase[J]. Plant Physiol, 1997, 114(4): 1547-1556
参考文献 41
189. Hobson GE, Richardson C,et al. Role of cell wall hydrolyses in fruit ripening. Plant Physiol, 1991, 42:
675-703
190. Fry SC, Smith RC. Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from
plants[J]. Biochamicial J, 1992, 282: 821-828
191. Redgwell RJ, Fry SC. Xyloglucan endotransglycosylase activity increases during kiwifruit ripening:
implications for fruit softening[J]. Plant Physiol, 1993, 103(4): 1399-1406
192. McDougall GJ, Fry SC. Xyloglucan oligosaccharides promotes growth and activated cellulase:
evidence for a role of cellulase in cell expansion[J]. Plant Physiol,1990, 93: 1042-1048
193. Siddiqui S,Bangerth F. Effect of pre-harvest application of calcium on flesh firmness and cell-wall
composition of apples-influence of fruit size[J]. J Hortic Sci, 1995, 70: 263-269
194. Lazan H, Selamat MK, ALi ZM.β-galactosidase,polygalacturonase and pectinesterase in differential
softening and cell wall modification during papaya fruit ripening[J]. Plant Physiol, 1995, 95:106-112
195. Andrews PK, Li S. Partial purification and characterization of β-galactosidase from sweet cherry, a
nonclimacteric fruit[J]. J Agric Food Chem, 1994, 42: 2177-2182
196. Ross GS, Redywell RJ, et al. Kiwifruit β-galactosidase:isolation and activity against specific fruit
cell-wall polysaccharides[J]. Planta, 1993, 189: 499-506
197. Carey AT, Hott K, et al. Tomato exo-(1→4)β-D-galactanase isolation change during ripening in
normal and mutant tomato fruit, and characterization of a related cDNA clone[J]. Plant Physiol, 1995,
108: 1099-1107
198. Ross GF, Wegrzyn TF, et al. Appleβ-galatosidase activity against cell wall polysacharides and
characterization of a related cDNA clone[J]. Plant Physiol, 1994, 106: 521-528
199. Rose JKC, Lee HH, et al. Expression of a divergent gene is fruit-specific and ripening-regulated[J].
Proc Natl Acad Sci USA, 1997, 94: 5955-5960
200. Harrison EP, Mc Queen-Mason ST, et al. Expression of six expansin genes in relation to extention
activity indeveloping strawberry fruit[J]. J Exp Bot, 2001, 52: 1437-1446
201. Hayama H, Shimada T, et al. Molecular cloning of a ripening-related expansin cDNA in peach :
evidence for no relationship between accumulation and change in fruit firmness during storage[J]. J
Plant Physiol, 2000, 157: 567-573
202. Brummel DA, Harpster MH, et al. Differential expression of expansin gene family members during
growth and ripening of tomato fruit[J]. Plant Mol Biol, 1999, 39: 161-169
203. Obenland D, Rose J, et al. The relationship between expansin expression and development of
mealiness in peaches[J]. Hortscience(abstract), 2001, 36: 596
204. Sato H, Theologis A. Cloning the mRNA encoding ACCsynthase –the key enzyme for enthylene
biosymthesis in plants[J]. Proc Natl Acad Sci USA, 1989, 86: 6621-6625
205. Yang SF, Hoffman NE. Ethylene biosynthesis and its regulation in higher plants[J]. Ann Rev Plant
Physiol, 1984, 35: 155-189
206. Wakoby N, Freeman S, et al. Pectinesterae, poiygalacturonase, and beta-galactosidase during
softenings of ethylene-treated kiwifruit. HortScience, 1992, 27(8): 900-902
207. De Haan I. Pectin conversions in peaches during cold storage. The Sourh African Industrial Chemist,
1957, 11: 26-34
42 MA 冷藏对油桃果实成熟软化的控制
208. Kramer M, Sanders R. Postharvest evaluation of transgenic tomatoes with reduced levels of
polygalacturonase, processing firmness and disease resistance [J]. Postharvest Biology and Technology,
1992, 1 (2): 411-419