摘要
【目的】通过旋磁场作用于慢性前列腺炎模型炎症介质变化的实验研究,探讨旋磁场对慢性前列腺炎的作用机制,探索慢性前列腺炎治疗的新途径。
【方法】选取36只体重500-800克的一周岁SD雄性大鼠,随机分为3组,按照魏氏法复制前列腺炎模型。完成建模后,分为对照组,不行任何处理;低磁组即每天暴露于0.2特斯拉(T),10±2 Hz的旋磁场中30min;高磁组即每天暴露于0.3特斯拉(T),10±2 Hz的旋磁场中30min。旋磁场治疗40天之后,观察光镜下前列腺组织的炎症表现和半定量免疫组化比较各组前列腺组织中白细胞介素-6(IL-6)和血管内皮细胞生长因子(VEGF)的含量变化。
【结果】光镜下发现,低磁组的大鼠前列腺组织的炎症表现较对照组轻;高磁组的大鼠前列腺组织的炎症表现较低磁组轻。低磁组的大鼠前列腺组织中IL-6(白细胞介素-6)含量明显低于对照组,差别具有统计学意义,p< 0.05。高磁组的大鼠前列腺组织中IL-6(白细胞介素-6)含量明显低于低磁组,差别具有统计学意义,p< 0.05。高磁组的大鼠前列腺组织中IL-6(白细胞介素-6)含量明显低于对照组,差别具有统计学意义,p< 0.05。低磁组的大鼠前列腺组织中VEGF(血管内皮细胞生长因子)含量明显低于对照组,差别具有统计学意义,p< 0.05。高磁组的大鼠前列腺组织中VEGF(血管内皮细胞生长因子)含量明显低于低磁组,差别具有统计学意义,p< 0.05。高磁组的大鼠前列腺组织中VEGF(血管内皮细胞生长因子)含量明显低于对照组,差别具有统计学意义,p< 0.05。
【结论】旋磁场对SD大鼠前列腺炎模型中炎症介质的含量有显著影响,同时减轻前列腺组织的炎性改变,提示旋磁场对前列腺炎可能存在一定的治疗作用。
【OBJECT】We take the research for the content changes of the inflammation media in the chronic prostatitis model caused by rotating magnetic field to treat of the mechanism of the affection of the rotating magnetic field to the chronic prostatitis and explore the new way to cure the chronic prostatitis.
【METHOD】Choose 36 1-year old male SD Rats which weigh 500-800 gram and make the prostatitis models. After creating the prostatitis models following the Wei’s way, we divide all of the rats into three groups on average randomly. They are control group which will never be therapied in any way, weak magnetic group which will be exposed in the 0.2T, 10±2 Hz rotating magnetic field for 30 min per day, intense magnetic group which will be exposed in the 0.3T, 10±2 Hz rotating magnetic field for 30 min per day. After 40 days therapy of the rotating magnetic field, we observe the inflammatory changes by the microscope and analysis the content changes of the (interlukin-6) and VEGF (vascular endothelial growth factor) in the prostate tissue of all the groups by the semi quantitative analysis of histochemistry.
【RESULT】The inflammatory expression in the prostate tissue of the weak magnetic group is slighter than it is in the prostate tissue of the control team. The inflammatory expression in the prostate tissue of the intense magnetic group is slighter than it is in the weak magnetic group. The content of IL-6 (interlukin-6) in the prostate tissue of the weak magnetic group is less than it is in the prostate tissue of the control magnetic group remarkably and statistically ,p<0.05. The content of IL-6 (interlukin-6) in the prostate tissue of the intense magnetic group is less than it is in the prostate tissue of the weak magnetic group remarkably and statistically, p<0.05. The content of IL-6 (interlukin-6) in the prostate tissue of the intense magnetic group is less than it is in the prostate tissue of the control group remarkably and statistically ,p<0.05.The content of VEGF (vascular endothelial growth factor) in the prostate tissue of the weak magnetic group is less than it is in the prostate tissue of the control magnetic group remarkably and statistically,p< 0.05. The content of VEGF (vascular endothelial growth factor) in the prostate tissue of the intense magnetic group is less than it is in the prostate tissue of the weak magnetic group remarkably and statistically,p< 0.05. The content of VEGF (vascular endothelial growth factor) in the prostate tissue of the intense magnetic group is less than it is in the prostate tissue of the control group remarkably and statistically,p< 0.05.
【CONCLUSION】The rotating magnetic field affects the content of the inflammatory in the SD rat prostatitis model remarkably ,and relieves the inflammation in the prostate tissue. This points out the rotating magnetic field can be helpful to therapy the prostatitis to some degree.
引文
[1] Jillian L. Capodice, Debra L. Bemis, Ralph Buttyan ,et al.Complementary and Alternative Medicine for Chronic Prostatitis/Chronic Pelvic Pain syndrome[J]. eCAM, 2005,2(4):495–501.
[2] 于 玲 娜 , 田 立 健 . 磁 场 对 健 康 人 血 细 胞 的 影 响 [J]. 中 华 理 疗 杂志,1994,17(1):19-21.
[3] 梁 路 光 , 赵 大 源 . 磁 场 对 在 鼠 血 液 流 变 学 的 影 响 [J]. 中 华 理 疗 杂志 ,1995,18(2):22-23. 常 汉 英 . 磁 场 对 瘢 痕 组 织 的 影 响 [J]. 中 华 理 疗 杂志,1997,20(1):19-22.
[4] 于玲娜.磁场对大鼠疤痕组织的实验研究[J].中华理疗杂志,1992,17(1): 19-19.
[5] Zhang Y,Xue Y,Zhang XY .Effects of 0.4 T rotating magnetic field exposure on density, strength, calcium and metabolism of rat thigh bones[J].Bioelectromagnetics, 2006 ,27(1):1-9.
[6] 夏绪刚.旋磁场拮抗脑缺血再灌注损伤中磷脂酶 A2 和内皮素作用的研究[J].中华理疗杂志,1997,20(1) :13-15.
[7] 阎秀英.旋磁场对血清铜蓝蛋白活性的影响[J].中华理疗杂志,1996,19(3): 210.
[8] 杨修益,丁克祥.磁场对人体自由基代谢相关成分的影响[J].中华理疗杂志,1996,19(4):212-213.
[9] 韩丽莎,王维.旋磁场对脑缺血再灌注损伤大鼠血液流变学的影响[J]. 中华理疗杂志,2000,23(3): 155-157.
[10] 韩丽莎, 韩利 .旋磁场对大鼠实验性缺血心肌保护作用的机制研究[J]. 中华物理医学与康复杂志,2003,25(11): 643-645.
[11] 王芳,韩丽莎. 旋磁场对糖尿病大鼠血糖及组织细胞学影响的观察[J]. 中国临床康复,2002, 6(3):376-376.
[12] 马燕燕, 林琳 .旋磁场对实验性糖尿病大鼠糖代谢用细胞形态的影响[J]. 中华实用医学,2002, 4(3):43-43.
[13] 周义生,李玉珍,高炳吾.旋磁场对胆结石病人胆汁中胆红素钙胆固醇 pH 影响的研究[J]. 生物磁学.2001.1(1):36-38.
[14] 郭春光,辜良媛.旋磁法在康复医学中的应用[J]. 现代康复,1999, 3 ( 6 ):25-26
[15] Chereiskaia NK,Gilinskaia NIu ,Lobanov AIu,,Physiotherapy with rotating pulse magnetic field in combined therapy of chronic obstructive pulmonary disease[J]. Vopr Kurortol Fizioter Lech Fiz Kult,2005,1(1):19-22.
[16] Jin HM, Zhan BY,Wang LL.Massage of transrectal heat rotating magnetic field in the treatment of obstinate hemospermia[J]. Zhonghua Nan Ke Xue. 2006 ,12(1):60-1, 65.
[17] 罗二平,焦李成.旋磁按摩结合药物灌注治疗慢性前列腺炎的临床观察[J]. 第四军医大学学报,2004,25(3):268-270.
[18] 魏武然, 张唯力 ,戴君勇,大鼠慢性非细菌性前列腺炎模型的建立[J].中国男科学杂志,2006,20(1):22-24
[19] Vaitkeviciene I, Vaitkevicius R, Paipaliene P,Morphometric analysis of pulpal myelinated nerve fibers in human teeth with chronic periodontitis and root sensitivity[J].Medicina (Kaunas). 2006;42(11):914-22
[20] Nickel J. Curtis, Chronic Prostatitis: An Infectious Disease? Infect Urol, 2000; 13(2):31-38
[21] Litwin MS. A review of the development and validation of the National Institutes of Health Chronic Prostatitis Symptom Index[J]. Urology, 2002, 60(6):14–8
[22] Nickel J. Curtis, Wolfgang Weidner. Chronic Prostatitis: Current Concepts and Antimicrobial Therapy [J]. Infect Urol, 2000, 13(5):22-28.
[23] Michael A.Tanner, Daniel Shoskes, Shahed A Sha,et al. Prevalence of Corynebacterial 16S rRNA Sequences in Patients with Bacterial and “Nonbacterial” Prostatitis[J]. J Clin Microbiol, 1999, 37(6): 1863–1870.
[24] Michel AP, Michael R .Mechanisms in Prostatitis/Chronic Pelvic Pain Syndrome [J] .J Urol, 2004, 172(1):839-845.
[25] Weidner W. Treating Chronic Prostatitis: Antibiotics No, a-Blockers Maybe [J]. Ann Intern Med, 2004, 141(8):639-40.
[26] Richard B. Alexander. Treatment of Chronic Prostatitis[J]. Nat Clin Pract Urol, 2004, 12(1):2-3
[27] Nickel J. Curtis, Wolfgang Weidner. Chronic Prostatitis: Current Concepts and Antimicrobial Therapy [J]. Infect Urol, 2000, 13(5):22-28.
[28] Ye ZQ, Cai D, Lan RZ, et al. Biofeedback therapy for chronic pelvic painsyndrome[J]. Asian J Androl, 2003,5(2):155–8.
[29] Han J, Pomeranz B, Stux G. Scientific bases of acupuncture analgesia [J]. Acupunct Electrother Res. 2002,27(1):1-14.
[30] John H, Ruedi C, Kotting S,et al. A new high frequency electrostimulation device to treatchronic prostatitis[J]. J Urol, 2004, 171(6):2389.
[31] Lee K.C, Jung P.B, Park H.S, et al. Transurethral needle ablation for chronic nonbacterial prostatitis[J], BJU international, 2002, 89(2):226-229.
[32] Kwon SM, Kim SI, Chun DC,et al,Development of rat prostatitis model by oral administration of isoflavone and its characteristics[J],Yonsei Med J, 2001 ,42(4):395-404.
[33] 亭梅.老年慢性支气管哮喘患者血清 TNF-a IL-6 水平及其临床意义[J],放射免疫学杂志.2005,18(6):440
[34] Yamanchi TK,lhara Y,Ogata A,et a1.Hypoxic stress induces cardiac myocyte-derived interleukin-6[J].Circulation,91(5):1520-4.
[35] 汪岩,赵忠文,王立波,前列腺液中细胞因子(IL-6、TNF-a)在慢性前列腺炎中的意义[J],白求恩医科大学学报,1999,25(4):402-403
[36] Neufeld G,Cohen T,Gengrinovitch S,et a1.Vascular endothelial growth factor and its receptors[J].FASEB J,1999,13(1):9
[37] Ferrara N . Vascular endothelial growth factor : molecular and biological aspects[J].Curt Top Microbiol Immunel,1999,237(1):1-3.
[38] Hareld F,Dvora k,Lawrence F,et a1.Vascular permeability factor, microvascular hypermeability and angiogenesis[J].Am J Pathology,1995,(146):1029
[39] 林立.血管生成的基因治疗[J].德国医学,1999,16(4):202-205。
[40] Brogi E, Schatteman G, Wu T,et al,Hypoxia-induced paracrine regulation of vascular endothelial growth factor receptor expression[J].J Clin Invest, 1996 ,97(2):469-76.
[41] Patil SD, Papadmitrakopoulos F, Burgess DJ.Concurrent delivery of dexamethasone and VEGF for localized inflammation control and angiogenesis[J].J Control Release, 2007,117(1):68-79.
[42] 夏圣,血管内皮细胞生长因子的研究进展[J],国外医学临床生物化学与检验学分册,1997,18(4):161-163.
[43] KurtG.Naber ,Wolfgang Weiner.chronic prostatitis-an infectious disease? Journal of Antimicrobial Chemotherapy, 2000, 46(2):157-161.
1. Jillian L. Capodice, Debra L. Bemis, Ralph Buttyan ,et al.Complementary and Alternative Medicine for Chronic Prostatitis/Chronic Pelvic Pain syndrome[J], eCAM, 2005,2(4):495–501.
2. Kurt G.Naber. prostatitis[J]. Nephrol Dial Transplant, 2001, 16(6):132-134.
3. Kurt G..Naber, Wolfgang Weiner.chronic prostatitis-an infectious disease? [J]. J Antimicrob Chemother, 2000, 46(1):157-161.
4. Nickel J. Curtis. Chronic Prostatitis: An Infectious Disease? [J]. Infect Urol, 2000, 13(2):31-38.
5. 叶章群,曾晓勇,慢性前列腺炎诊疗进展[J].中华男科学,2003,9(7):483-488
6. Litwin MS. A review of the development and validation of the National Institutes of Health Chronic Prostatitis Symptom Index[J]. Urology, 2002, 60(6):14–8
7. Johns.Wolfson, David C.Hooper.Treatment of Genitourinary Tract Infections with Fluoroquinolones:Activity In Vitro Pharmacokinetics, and Clinical Efficacy in Urinary Tract Infections and Prostatitis[J]. Antimicrob Agents Chemother, 1989, 33(10): 1655-1661.
8. Nickel J. Curtis, Wolfgang Weidner. Chronic Prostatitis: Current Concepts and Antimicrobial Therapy [J]. Infect Urol, 2000, 13(5):22-28.
9. Hiep N'Guyen Trong, Anne-Laure Prunier, Roland Leclercq ,et al.Hypermutable and Fluoroquinolone-Resistant Clinical Isolates of Staphylococcus aureus[J]. Antimicrob Agents Chemother, 2005, 49(5): 2098–2101.
10. Juan P.Horcajada, Jordi Vila, Antonio Moreno-Martinez,et al. Molecular epidemiology and evolution of resistance to quinolones in escherichia coli after prolonged administration of ciprofloxacin in patients with prostatitis[J]. J Antimicrob Chemother, 2002, 49(1):55-59.
11. Litwin MS, McNaughton-Collins M, Fowler FJ Jr, et al. The National Institutes of Health chronic prostatitis symptom index: development and validation of a new outcome measure [J].Chronic Prostatitis Collaborative Research Network. J Urol, 1999, 162(2):369–75.
12. Weidner W. Treating Chronic Prostatitis: Antibiotics No, a-Blockers Maybe [J]. Ann Intern Med, 2004, 141(8):639-40.
13. Richard B. Alexander. Treatment of Chronic Prostatitis[J]. Nat Clin Pract Urol, 2004, 12(1):2-3
14. Richard B. Alexander, Kathleen J. Ciprofloxacin or Tamsulosin in Men with Chronic Prostatitis/Chronic Pelvic Pain Syndrome [J]. Ann Intern Med, 2004, 141(8):581-589.
15. Ashar.Shahed, Daniel A.Shoskes. Oxidative Stress in Prostatic Fluid of Patients with Chronic Pelvic Pain Syndrome: Correlation with Gram Positive Bacterial Growth and Treatment Response [J]. J Androl, 2000, 21(5):669-675.
16. Michael A.Tanner, Daniel Shoskes, Shahed A Sha,et al. Prevalence of Corynebacterial 16S rRNA Sequences in Patients with Bacterial and “ Nonbacterial ” Prostatitis[J]. J Clin Microbiol, 1999, 37(6): 1863–1870.
17. Donald E.Riley, Richard E. Berger, David C. Miner, et al. Diverse and Related 16S rRNA-Encoding DNA Sequences in Prostate Tissues of Men with Chronic Prostatitis[J]. J Clin Microbiol, 1998, 36(6): 1646–1652.
18. Luzzi GA. Whither “Chronic prostatitis”? [J]. Sex. Transm. Infect, 2005, 81(2):96-7.
19. Ye ZQ, Cai D, Lan RZ, et al. Biofeedback therapy for chronic pelvic pain syndrome[J]. Asian J Androl, 2003,5(2):155–8.
20. Han J, Pomeranz B, Stux G. Scientific bases of acupuncture analgesia [J]. Acupunct Electrother Res. 2002,27(1):1-14.
21. David M. Hough, Keith H, Wittenberg. Chronic Perineal Pain Caused byPudendal Nerve Entrapment:Anatomy and CT- Guided Perineural Injection Technique[J]. AJR, 2003(8):181.
22. Chen R, Nickel JC. Acupuncture ameliorates symptoms in men with chronic prostatitis/chronic pelvic pain syndrome[J]. Urology 2003, 61(6):1156–9.
23. Honjo H, Kamoi K, Naya Y, et al.Effects of acupuncture for chronic pelvic pain syndrome with intrapelvicvenous congestion: preliminary results [J]. Int J Urol ,2004,11(8):607–12.
24. John H, Ruedi C, Kotting S,et al. A new high frequency electrostimulation device to treatchronic prostatitis[J]. J Urol, 2004, 171(6):2389.
25. Lee K.C, Jung P.B, Park H.S, et al. Transurethral needle ablation for chronic nonbacterial prostatitis[J]. BJU international, 2002, 89(2):226-229.
26. Kaplan SA, Volpe MA, Te AE. A prospective, 1-year trial using saw palmetto versus finasteride in the treatment of category III prostatitis/chronic pelvic pain syndrome[J]. J Urol 2004 ,171(1):284–8.
27. Reissigl A, Pointner J, Djavan B,et al .Multi centre study to evaluate the safety and efficacy of phytotherapeuticum permixon in the treatment of chronic prostatitis/chronic pelvic pain syndrome[J].European Urology Supplements, 2003, 2(1) : 15-15
28. Rugendorff EW, Weidner W, Ebeling L,et al. Results of treatment with pollen extract (Cernilton N) in chronic prostatitis and prostatodynia[J].Br J Urol,1993,71(4):433–8
29. Sri KV, Kondaiah A, Ratna JV, et al. Preparation and characterization of quercetin and rutin cyclodextrin inclusion complexes[J]. Drug Dev Ind Pharm,2007,33(3):245-53.
30. Alia M, Mateos R, Ramos S, et al. Influence of quercetin and rutin ongrowth and antioxidant defense system of a human hepatoma cell line (HepG2)[J]. Eur J Nutr,2006,45(1):19-28
31. Joseph Jankovic. Botulinum toxin in clinical practice [J]. J Neurol Neurosurg Psychiatry, 2004, 75(5):951–957.
32. 张振江,张瑞刚 ,佘在霞.心理治疗在慢性非细菌性前列腺炎治疗中的作用[J].中国医学理论与实践.2003,3(3):327.
33. 王晓臣.慢性前列腺炎的治疗概况[J]. 中国性科学,2005,14(6):29-36