摘要
论文概述了共振散射技术的基本概念、研究现状及应用。建立一个新的无荧光探针的共振散射技术研究卡波姆及羧甲基纤维素钠(CMC-Na)的自聚集作用,并用于卡波姆及CMC-Na的含量测定。本论文共分为三章。
第一章本章概述了光散射概念及分类,共振光散射技术基本概念、特点、发展及其在生物大分子、药物分析、金属离子分析等方面的应用,从而提出本课题研究的意义。
第二章本章主要用共振散射技术研究不同型号卡波姆在酸性条件下(pH=2.0)的自聚集作用。实验中考察了不同pH值、不同缓冲介质、有机溶剂的含量、稳定性、离子强度、干扰物质等的影响。优选出条件为pH=2.0磷酸盐缓冲液。用共振散射技术研究卡波姆的自聚集作用具有较好的稳定性,在一定范围内不受离子强度、干扰物质的影响。
卡波姆940在20～120μg·mL~(-1)之间线性关系良好,相关系数r=0.9997。进一步用于卡波姆940样品的含量测定,其含量分别为0.993、1.006、1.032、0.986 mg·mL~(-1),与实际含量接近,RSD(%)分别为0.57、0.82、0.42、0.56。平均加样回收率(%)分别为100.9、98.8、100.9、100.9,RSD(%)分别为0.98、1.64、1.11、2.09。结果表明,用共振散射方法分析卡波姆940样品,方法简便、快速,灵敏度高,具有较好的准确度和重复性,并能在较大限度范围内排除干扰。该法可用于样品中卡波姆940的含量测定。
卡波姆971P在50～300μg·mL~(-1)之间线性关系良好,相关系数r=0.9996。进一步应用于卡波姆971P样品的含量测定,其含量分别为0.980、0.956、1.000、1.012 mg·mL~(-1),与实际含量接近,RSD(%)分别为1.00、1.37、0.59、1.97。平均加样回收率(%)分别为96.2、97.3、96.8、97.4,RSD(%)分别为0.94、0.42、1.66、1.14。结果表明,用共振散射方法分析卡波姆971P样品,灵敏度较高,方法简便、快速,具有较好的准确度和重复性,并能在较大限度范围内排除干扰。该法可用于样品中卡波姆971P的含量测定。
卡波姆974P在20～300μg·mL~(-1)之间线性关系良好,相关系数r=0.9994。进一步应用于抗生素缓释凝胶、盐酸达克罗宁凝胶中卡波姆974P的含量测定。抗生素缓释凝胶中卡波姆974P的含量分别为37.45、37.33、37.33 mg·g~(-1),RSD(%)分别为1.13、1.04、1.34。平均加样回收率(%)分别为100.6、95.0、97.2,RSD(%)分别为1.35、0.52、2.36。盐酸达克罗宁凝胶中卡波姆974P的含量分别为13.69、13.52、13.06 mg·g~(-1),RSD(%)分别为1.40、1.03、0.39。平均加样回收率(%)分别为98.0、99.1、95.6,RSD(%)分别为1.22、0.77、1.19。结果表明,用共振散射方法分析样品中的卡波姆974P,灵敏度较高,方法简便、快速,具有较好的准确度和重复性。该法可用于样品中卡波姆974P的含量测定。
第三章本章主要用共振散射技术研究CMC-Na在酸性条件下(pH=2.0)的自聚集作用。实验中考察了不同pH值,不同缓冲介质,有机溶剂的含量,稳定性,离子强度,干扰物质等的影响。优选出条件为pH=2.0磷酸盐缓冲液。用共振散射技术研究CMC-Na的自聚集作用具有较好的稳定性,在一定范围内不受离子强度、干扰物质的影响。同时,发现其在10～200μg·mL~(-1)之间线性关系良好,相关系数r=0.9993。进一步用于羧甲基纤维素钠滴眼液、甲硝唑灌肠液及CMC-Na样品中CMC-Na的含量测定。羧甲基纤维素钠滴眼液中CMC-Na的含量分别为1.937、1.941、1.928 mg·支~(-1),RSD(%)分别为2.16、2.67、1.58。平均加样回收率(%)分别为95.3、103.7、95.0,RSD(%)分别为1.08、1.26、1.58。甲硝唑灌肠液中CMC-Na的含量分别为1.900、2.032、1.940 mg·mL~(-1),RSD(%)分别为0.36、0.97、1.27。平均加样回收率(%)分别为95.7、103.7、100.3,RSD(%)分别为0.76、1.26、0.71。CMC-Na样品中CMC-Na的含量分别为1.040、1.013、1.035 mg·mL~(-1),RSD(%)分别为0.28、0.90、0.37。平均加样回收率(%)分别为99.3、102.8、98.4,RSD(%)分别为1.62、0.84、1.20。结果表明,用共振散射技术分析样品中的CMC-Na,灵敏度高,方法简便、快速,具有较好的准确度和重复性。该法可用于样品中CMC-Na的含量测定。
The basic concept,current situation and application of resonance light scattering (RLS)were summarized. A novel free-probe study of carbomers and sodium carboxymethylcellulose (CMC-Na)was established based on their self-aggregations by RLS,and this technique was used for the assay of carbomers and CMC-Na in samples. This dissertation concretely consist of the following three chapters.
Chapter 1
The concept and classification of light scattering and the basic concepts ,characteristics,development and applications such as biological macromolecules,drug and metal ions analysis of RLS were summarized.And then the significance of these researches was put forward.
Chapter 2
In this chapter,the self-aggregations of different types of carbomers were discussed by RLS in the acidic condition(pH = 2.0). The main influencing factors such as pH values, buffer medium,content of organic solvent,stability,ionic strength and interfering substances were investigated in detail. Phosphate buffer(pH = 2.0)was obtained as optimum conditions.In the study of carbomers based on their self-aggregations by RLS,good stability was obtained and no interference was presented in the detection when adding ionic and interfering substances.
The RLS intensity was well proportional to the concentration of carbomer 940 over the wide range 20～120μg·mL~(-1) with r=0.9997 in the optimum conditions. The contents of carbomer 940 samples,which were almost identical to the actual ones,were analysed with the results as follows:0.993,1.006,1.032,0.986 mg·mL~(-1),and RSD(%)were 0.57,0.82,0.42,0.56,respectively. The average recoveries(%)were 100.9,98.8,100.9,100.9,and RSD(%)were 0.98,1.64,1.11,2.09,respectively. The results proved that the carbomer 940 samples analysed by the RLS owned the characteristics of easy operation,rapidity,sensitivity,good accuracy and repeatability,and also could eliminate the interfering substances in the large range. Based on this study,RLS can be used for the determination of carbomer 940 in the samples.
The RLS intensity was well proportional to the concentration of carbomer 971P over the wide range 50～300μg·mL~(-1) with r=0.9996 in the optimum conditions. The contents of carbomer 971P samples,which were almost identical to the actual ones,were analysed with the results as follows:0.980,0.956,1.000,1.012 mg·mL~(-1),and RSD(%)were 1.00,1.37,0.59,1.97,respectively. The average recoveries(%)were 96.2,97.3,96.8,97.4, and RSD(%)were 0.94,0.42,1.66,1.14,respectively. The results proved that the carbomer 971P samples analysed by RLS owned the characteristics of easy operation,rapidity,sensitivity,good accuracy and repeatability,and also could eliminate the interfering substances in the large range. Based on this study,RLS can be used for the determination of carbomer 971P in the samples.
The RLS intensity was well proportional to the concentration of carbomer 974P over the wide range 20～300μg·mL~(-1) with r=0.9994 in the optimum conditions. And the technique was used for analysing carbomer 974P in antibiotics sustained release gel and dyclonine hydrochloride gel. The contents of carbomer 974P in antibiotics sustained release gel were analysed with the results as follows:37.45,37.33,37.33 mg·mg~(-1),and RSD(%)were 1.13,1.04,1.34,respectively. The average recoveries(%)were 100.6,95.0,97.2, and RSD(%)were 1.35,0.52,2.36,respectively. The contents of carbomer 974P in dyclonine hydrochloride gel were analysed with the results as follows:13.69,13.52,13.06 mg·g~(-1),and RSD(%)were 1.40,1.03,0.39,respectively. The average recoveries(%) were 98.0,99.1,95.6,and RSD(%)were 1.22,0.77,1.19,respectively. The results proved that the carbomer 974P samples analysed by the RLS owned the characteristics of easy operation,rapidity,sensitivity,good accuracy and repeatability,and also could eliminate the interfering substances in the large range. Based on this study,RLS can be used for the determination of carbomer 974P in the samples.
Chapter 3
In this chapter,the self-aggregation of CMC-Na was discussed by RLS in the acidic condition(pH = 2.0). The main influencing factors such as pH values,buffer medium,content of organic solvent,stability,ionic strength and interfering substances were investigated in detail. Phosphate buffer (pH = 2.0) was obtained as optimum conditions. In the study of CMC-Na based on its self-aggregation by RLS,good stability was obtained and no interference was presented in the detection when adding ionic and interfering substances. It was found that the RLS intensity was well proportional to the concentration of CMC-Na over the wide range 10～200μg·mL~(-1) with r=0.9993 in the optimum conditions. And then the technique was used for analysing CMC-Na in CMC-Na eye drops,metronidazole clyster liquid and CMC-Na samples. The contents of CMC-Na in CMC-Na eye drops were analysed with the results as follows:1.937,1.941,1.928 mg·mL~(-1),and RSD(%)were 2.16,2.67,1.58,respectively. The average recoveries (%) were 95.3,103.7,95.0,and RSD(%)were 1.08,1.26,1.58,respectively. The contents of CMC-Na in metronidazole clyster liquid were analysed with the results as follows:1.900,2.032,1.940 mg·g~(-1),and RSD(%)were 0.36,0.97,1.27,respectively. The average recoveries(%)were 95.7,103.7,100.3,and RSD(%)were 0.76,1.26,0.71,respectively. The contents of CMC-Na in CMC-Na samples were analysed with the results as follows:1.040,1.013,1.035 mg·g~(-1),and RSD(%)were 0.28,0.90,0.37,respectively. The average recoveries(%)were 99.3,102.8,98.4,and RSD(%)were 1.62,0.84,1.20,respectively. The results proved that the CMC-Na samples analysed by RLS owned the characteristics of easy operation,rapidity,sensitivity,good accuracy and repeatability,and also could eliminate the interfering substances in the large range. Based on this study,RLS can be used for the determination of CMC-Na in the samples.
引文
[1]赵清诚.基础物理学[M].北京:中国医药科技出版社,2002:248.
[2]彭敬东.共振瑞利散射光谱在天然药物分析中的应用研究[D].西南师范大学,2005.
[3]左榘.散射原理及其在高分子科学中的应用[M].郑州:河南科学技术出版社,1994:1.
[4]王惠英.光散射技术及其在药物生物大分子中的应用研究[D].西南大学,2007.
[5] Cao Q E,Ding Z T,Fang R B,et al. A sensitive and rapid method for the determination of protein by the resonance Rayleigh light-scattering technique with pyrogallol Red[J]. Analys,2001,126(8):1444.
[6] Pasternack R F,Collings P J. Resonance Light Scattering:A New Technique for Studying Chromophore Aggregation[J]. Science,1995,269:935-939.
[7] Huang C Z,Li Y F,Hu X L,et al. Three-dimensional spectra of the long-range assembly of Nile Blue sulfate on the molecular surface of DNA and determination of DNA by light-scattering[J]. Anal Chim Acta,1999,395(1/2):187-197.
[8] Yang G. Study on the interaction of spectroscopicprobes with bioactive substances– reaction mechanism,analytical application and preparation[D]. Dissertation of Beijing University,2000.
[9] Lu W,Fernández Band B S,Yu Y,et al. Resonance light scattering and derived techniques in analytical chemistry:past,present,and future[J]. Microchim Acta,2007,158(1/2):29-58.
[10] Anglistera J,Steinberga I Z. Depolarized rayleigh light scattering in absorption bands measured in lycopene solution[J]. Chem Phys Lett,1979,65(1):50-54.
[11] Pasternack R F,Bustamante C,Collings P J,et al. Porphyrin assemblies on DNA as studied by a resonance light-scattering technique[J]. J Am Chem Soc,1993,115(13):5393-5399.
[12] Pasternack R F,Schaefer K F,Hambright P. Resonance light-scattering studies of porphyrin diacid aggregates[J]. Inorg Chem,1994,33(9):2062-2065.
[13] de Paula J C,Robblee J H,Pasternack R F. Aggregation of chlorophyll a probed byresonance light scattering spectroscopy[J]. Biophys J,1995,68(1):335-341.
[14]唐宁莉,周炎宏.蛋白质-四羧基铜酞菁体系的共振散射行为及其分析应用[J].分析试验室,2009,28(2):70-73.
[15] Tang N L,Peng J Y. Determination of proteins with tetracarboxy manganese(II) phthalocyanine by resonance light scattering technique[J]. Spectrochim Acta A,2008,71(4):1246-1249.
[16] Wu L H,Mu D,Gao D J,et al. Determination of protein by resonance light scattering technique using dithiothreitol–sodium dodecylbenzene sulphonate as probe[J]. Spectrochim Acta A,2009,72(1):178-181.
[17] Liu Y,Yang J H,Liu S F,et al. Resonance light scattering technique for the determination of protein with rutin and cetylpyridine bromide system[J]. Spectrochim Acta A,2005,61(4):641-646.
[18] Gao D J,He N,Tian Y,et al. Determination of bovine serum albumin using resonance light scattering technique with sodium dodecylbenzene sulphonate-cetyltrimethylamm- onium bromide probe[J]. Spectrochim Acta A,2007,68(3):573-577.
[19] Zhan G Q,Zhang L X,Li C Y. Resonance light scattering spectral method for the determination of serum albumin with the interaction of neutral red–sodium dodecyl sulfonate[J]. Colloid Surface B,2009,71(1):84-87.
[20]李燕,宋桂兰,卢燕,等.荧光镓-SDS共振瑞利散射法测定蛋白质的研究[J].分析试验室,2009,28(3):55-58.
[21] Chen Y H,Tian Y,Gao D J,et al. Determination of serum albumin in the presence of poly(diallyldimethylammonium chloride)by resonance light scattering technique[J]. Spectrochim Acta A,2007,66(4/5):1011-1015.
[22] Chen H Q,Xu F G,Hong S,et al. Quantitative determination of proteins at nanogram levels by the resonance light-scattering technique with composite nanoparticles of CdS/PAA[J]. Spectrochim Acta A,2006,65(2):428-432.
[23]彭丽,王炎英,薛红波,等. Ru-PAA复合纳米粒子与蛋白质相互作用的光谱研究及其应用[J].分析科学学报,2009,25(6):677-681.
[24] Peng L,Li C Y,Xue H B,et al. Ultrasensitive determination of serum albumin usingresonance light scattering based on ZnS-polyacrylic acid nanoparticles[J]. Spectrochim Acta A,2010,75(5):1497-1500.
[25] Li L,Song G W,Fang G R. Determination of bovine serum albumin by a resonance light-scattering technique with the mixed-complex La(Phth)(phen)3+[J]. J Pharmaceut Biomed,2006,40(5):1198-1201.
[26] Pan H C,Tao X C,Mao C J,et al. Aminopolycarboxyl-modified Ag2S nanoparticles:Synthesis,characterization and resonance light scattering sensing for bovine serum albumin[J]. Talanta,2007,71(1):276-281.
[27] Liu S P,Yang Z,Liu Z F,et al. Resonance Rayleigh-scattering method for the determination of proteins with gold nanoparticle probe[J]. Anal Biochem,2006,353(1): 108-116.
[28] Zhang S H,Fan Y S,Feng S,et al. Microdetermination of proteins by resonance light scattering technique based on aggregation of ferric nanoparticles[J]. Spectrochim Acta A,2009,72(4):748-752.
[29] Feng S L,Pan Z H,Fan J. Determination of proteins at nanogram levels with Bordeaux red based on the enhancement of resonance light scattering[J]. Spectrochim Acta A,2006,64(3):574-579.
[30] Dong L J,Li Y,Zhang Y H,et al. A flow injection sampling resonance light scattering system for total protein determination in human serum[J]. Spectrochim Acta A,2007,66(4/5):1317-1322.
[31]王明瑞,董学芝,邓翠贞.光谱法研究二甲酚橙-Cu(ll)配合物与蛋白质的相互作用[J].分析试验室,2009,28(1):76-79.
[32] Huang C Z,Lu W,Li Y F,et al. On the factors affecting the enhanced resonance light scattering signals of the interactions between proteins and multiply negatively charged chromophores using water blue as an example[J]. Anal Chim Acta,2006,556(2):469-475.
[33]敖登高娃,阿娟,陶玉龙.吐温-20敏化甲基蓝-蛋白质散射光谱研究与应用[J].分析科学学报,2009,25(4):493-496.
[34]高阳,毕绍丹.蛋白质与玫瑰桃红R共振瑞利散射的研究及应用[J].广东微量元素科学,2009,16(7):21-24.
[35]刘健,刘忠芳,胡小莉,等.用硫酸软骨素A作探针共振瑞利散射及共振非线性散射法测定蛋白质[J].化学学报,2010,68(12):1210-1216.
[36] Cai C Q,Chen X M. Determination of nanograms of proteins based on the amplified resonance light scattering signals of Tichromine[J]. Spectrochim Acta A,2010,75(3): 1057-1060.
[37] Chen Z G,Liu J B,Han Y L. Rapid and sensitive determination of proteins by enhanced resonance light scattering spectroscopy of sodium lauroyl glutamate[J]. Talanta, 2007,71(1):1246-1251.
[38] Dong L J,Chen X G,Hu Z H. Total internal reflected resonance light scattering determination of protein in human blood serum at water / tetrachloromethane interface with Arsenazo-TB and Cetyltrimethylammonium bromide[J]. Talanta,2007,71(2):555-560.
[39]胡勇,扶雄,陈旭东,等.山梨酸钾与蛋白质相互作用的荧光和共振光散射光谱研究[J].中山大学学报,2009,48(6):73-79.
[40]胡勇,扶雄,陈旭东,等.聚乙二醇与蛋白质相互作用的荧光和共振散射光谱研究[J].现代食品科技,2009,25(4):353-357.
[41] Chen Z G,Liu G L,Chen M H,et al. Determination of nanograms of proteins based on decreased resonance light scattering of zwitterionic gemini surfactant[J]. Anal Biochem, 2009,384(2):337-342.
[42]立松,王颖臻,曹秋娥,等.钙指示剂羧酸钠-SDS体系共振瑞利散射法测定蛋白质[J].中国卫生检验杂志,2009,19(6):1284-1286.
[43] Huang K J,Wei C Y,Shi Y M,et al. The investigation of the interactions between CdSe quantum dots and human serum albumin by resonance Rayleigh scattering and second-order scattering spectra[J]. Spectrochim Acta A,2010,75(3):1031-1035.
[44]韦小玲,张玉兰,蒋治良.一种检测痕量IgG的免疫纳米银共振散射光谱探针[J].光谱学与光谱分析,2009,29(6):1640-1642.
[45] Wei X L,Liang A H,Zhang S S,et al. A selective resonance scattering assay for immunoglobulin G using Cu(II)-ascorbic acid-immunonanogold reaction[J]. AnalBiochem,2008,380(2):223-228.
[46] Jiang Z L,Wang S Mi,Liang A H,et al. A highly sensitive resonance scattering spectral assay for IgG using Fehling reagent–glucose–immunonanogold reaction[J]. Talanta,2009,77(3):1191-1196.
[47] Zhao H W,Huang C Z,Li Y F. Immunoassay by detecting enhanced resonance light scattering signals of immunocomplex using a common spectrofluorometer[J]. Talanta,2006,70(3):609-614.
[48]黄珊,肖琦,何治柯,等. CdSe量子点探针共振光散射法检测溶菌酶[J].高等学校化学学报,2009,30(10):1951-1955.
[49] Wang X Y,Xu Y,Xu X,et al. Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles[J]. Talanta,2010,82(2):693-697.
[50]敖登高娃,刘俊轶,张晓辉.甲基蓝与血红蛋白相互作用的共振瑞利散射光谱研究及小分子的影响[J].分析试验室,2010,29(12):39-42.
[51] Gao D J,Tian Y,Liang F H,et al. Investigation on hyperin-cetyltrimethylammonium bromide-fibronectin system by resonance light-scattering technique[J]. Spectrochim Acta A,2007,66(1):52-57.
[52]孙双姣,梁爱惠,蒋治良,等.免疫复合物共振散射光谱法测定人血纤维蛋白原[J].应用化学,2009,26(4):451-455.
[53]李正平,王磊,高瑞广,等.共振光散射测定辣根过氧化物酶[J].河北大学学报, 2009,29(3):173-176.
[54]蒋治良,张玉兰,梁爱惠,等.痕量甲胎蛋白的免疫纳米金催化-氧化亚铜微粒共振散射光谱分析[J].高等学校化学学报,2009,30(6):1109-1115.
[55]刘庆业,韦丽丽,梁爱惠,等.痕量铜蓝蛋白的免疫纳米金催化-氧化亚铜微粒共振散射光谱分析[J].化学学报,2009,67(17):2013-2018.
[56]杨培慧,韦炜,蔡怀鸿,等.抗-转铁蛋白-纳米金探针的制备及对转铁蛋白免疫识别的光谱分析[J].光谱学与光谱分析,2009,29(5):1398-1401.
[57] Gao D J,Tian Y,Ding L,et al. Determination of human complement factor C4 using resonance light-scattering technique with sodium dodecylbenzene sulphonate probe[J].Spectrochim Acta A,2006,64(2):430-434.
[58] Chen Z G,Liao X H,Zhu L,et al. Micro-determination of nucleic acids with a simple probe manganese chloride based on the fine enhanced resonance light scattering[J]. Spectrochim Acta A,2007,68(2):263-268.
[59] Wu X,Sun S N,Yang J H,et al. Study on the interaction between nucleic acid and Eu3+–oxolinic acid and the determination of nucleic acid using the resonance light scattering technique[J]. Spectrochim Acta A,2005,62(4/5):896-901.
[60] Jia Z,Yang J H,Wu X,et al. The sensitive determination of nucleic acids using resonance light scattering quenching method[J]. Spectrochim Acta A,2006,64(3):555-559.
[61] Li J,Kang J,Lu J,et al. Determination of calf thymus DNA using resonance light-scattering quenching method based on the terbium(Ш)(Tb3+)/europium(Ш)(Eu3+)– quercetin system[J]. J Lumin,2009,129(9):906-911.
[62]秦芳,蒋玲玲,刘维舟,等. Ru(phen)2dppx2+-SDBS-DNA体系共振瑞利散射光谱及其分析应用研究[J].分析科学学报,2009,25(1):63-66.
[63] Wu X,Wang Y B,Wang M Q,et al. Determination of nucleic acids at nanogram level using resonance light scattering technique with Congo Red[J]. Spectrochim Acta A,2005, 61(3):361-366.
[64] Fang F,Zheng H,Li L,et al. Determination of nucleic acids with a near infrared cyanine dye using resonance light scattering technique[J]. Spectrochim Acta A,2006,64(3):698-702.
[65] Chen X M,Cai C Q,Luo H A,et al. Study on the resonance light-scattering spectrum of anionic dye xylenol orange-cetyltrimethylammonium-nucleic acids system and determination of nucleic acids at nanogram levels[J]. Spectrochim Acta A,2005,61(9):2215-2220.
[66] Cheng Y Q,Li Z P,Su Y Q,et al. Ferric nanoparticle-based resonance light scattering determination of DNA at nanogram levels[J]. Talanta,2007,71(4):1757-1761.
[67] Zou Q C,Yan Q J,Song G W,et al. Detection of DNA using cationic polyhedraloligomeric silsesquioxane nanoparticles as the probe by resonance light scattering technique[J]. Biosens Bioelectron,2007,22(7):1461-1465.
[68] Yu J H,Li B,Dai P,et al. Study on the interaction among molecules and the determination of DNA by light scattering with a new type rhodanine derivative[J]. Spectrochim Acta A,2009,74(4):875-880.
[69] Chen Z G,Zhang T Y,Han Y L. Resonance light scattering spectroscopy study of interaction between norfloxacin and calf thymus DNA and its analytical application[J]. Spectrochim Acta A,2006,65(3/4):919-924.
[70]刘绍璞,王芬,刘忠芳,等.盐酸表柔比星与核酸相互作用的共振瑞利散射光谱研究及其分析应用[J].化学学报,2007,65(10):962-970.
[71]杨昌英,刘义,曾芳,等.两种荧光探针法研究三种非甾体抗炎药与DNA的相互作用[J].化学学报,2007,65(18):2076-2080.
[72]刘绍璞,胡小莉,刘忠芳,等.用共振Rayleigh散射光谱研究盐酸氯丙嗪和盐酸异丙嗪与核酸相互作用[J].中国科学B辑:化学,2005,35(4):312-319.
[73]杨兰玲,唐宁莉,王秋红.四羧基铝酞菁与硫酸庆大霉素相互作用的共振散射光谱及其分析应用[J].分析试验室,2009,28(8):79-81.
[74]王明霞,刘忠芳,胡小莉,等.达旦黄与氨基糖苷类抗生素相互作用的共振瑞利散射光谱研究及其应用[J].理化检验-化学分册,2006,42(1):16-21.
[75] Liu Z W,Liu S P,Wang L,et al. Resonance Rayleigh scattering and resonance non-linear scattering method for the determination of aminoglycoside antibiotics with water solubility CdS quantum dots as probe[J]. Spectrochim Acta A,2009,74(1):36-41.
[76] Lu X,Zhang D,Liu C W,et al. Development of a non-derivatization high- performance liquid chromatography method with resonance Rayleigh scattering detection for the detection of sisomicin in rat serum[J]. J Chromatogr B,2009,877(31):4022-4026.
[77] Duan H,Liu Z F,Liu S P,et al. Resonance Rayleigh scattering,second-order scattering and frequency doubling scattering methods for the indirect determination of penicillin antibiotics based on the formation of Fe3[Fe(CN)6]2 nanoparticles[J]. Talanta, 2008,75(5):1253-1259.
[78] Fu S H,Liu Z F,Liu S P,et al. Study on the resonance Rayleigh scattering spectra of the interactions of palladium(II)–cephalosporins chelates with 4,5-dibromofluorescein and their analytical applications[J]. Anal Chim Acta,2007,599(2):271-278.
[79]郑虎.药物化学[M].北京:人民卫生出版社,2005:254.
[80]魏小琴,刘忠芳,刘绍璞.四环素类抗生素-钨酸钠-乙基紫体系的共振瑞利散射光谱及其分析应用[J].化学学报,2006,64(6):521-526.
[81] Bing N,Luo H Q,Liu S P. Resonance Rayleigh scattering study of the inclusion complexation of chloramphenicol withβ-cyclodextrin[J]. Talanta,2005,66(2):495-500.
[82]唐晓玲,刘忠芳,刘绍璞,等.苯海拉明与赤藓红相互作用的吸收、荧光和共振瑞利散射光谱及其分析应用[J].中国科学B辑:化学,2007,37(1):68-75.
[83] Xu D P,Liu S P,Liu Z F,et al. Determination of verapamil hydrochloride with 12-tungstophosphoric acid by resonance Rayleigh scattering method coupled to flow injection system[J]. Anal Chim Acta,2007,588(1):10-15.
[84]刘梯楼,孙双姣,袁利萍,等.磷钼蓝共振散射光谱分析法测定抗坏血酸[J].光谱实验室,2010,27(5):2060-2063.
[85]李海霞,王卫华,蒋晔,等.共振瑞利散射法测定微量唑来膦酸[J].中国医药工业杂志,2008,39(12):930-932.
[86] Qin M Y,Liu S P,Liu Z F,et al. Resonance Rayleigh scattering spectra, non-linear scattering spectra of tetracaine hydrochloride–erythrosin system and its analytical application[J]. Spectrochim Acta A,2009,71(5):2063-2068.
[87]严军,刘忠芳,刘绍璞.汞(Ⅱ)-槲皮素螯合物共振瑞利散射光谱法测定槲皮素[J].分析化学,2007,35(1):123-126.
[88] Liu S P,Yang Z,Liu Z F,et al. Resonance Rayleigh scattering study on the interaction of gold nanoparticles with berberine hydrochloride and its analytical application[J]. Anal Chim Acta,2006,572(2):283-289.
[89]江珊珊,刘忠芳,胡小莉,等.秋水仙碱水解产物与某些酸性磺酞类染料相互作用的共振瑞利散射光谱及其分析应用[J].化学学报,2009,67(14):1635-1642.
[90]张书然,杨季冬.共振瑞利散射光谱法对盐酸异丙嗪与盐酸氯丙嗪的同时测定[J].分析测试学报,2009,28(12):1362-1367.
[91] Cui Y R,Cui F L,Wang L,et al. Determination of lead in Yellow River using ammonium molybdate as a molecular probe by resonance light scattering technique[J]. J Lumin,2008,128(10):1719-1724.
[92] Fan Y,Long Y F,Li Y F. A sensitive resonance light scattering spectrometry of trace Hg2+ with sulfur ion modified gold nanoparticles[J]. Anal Chim Acta,2009,653(2):207-211.
[93]刘云富,李贵荣,谭广辉.砷(V)—钼酸铵—碘绿的共振瑞利散射光谱及其应用研究[J].广东微量元素科学,2010,17(9):60-65.
[94]钱纯.镍(Ⅱ)-1-(2-吡啶偶氮)-2-萘酚-十二烷基苯磺酸钠体系共振光散射法测定痕量镍[J].理化检验-化学分册,2009,45(4):431-433.
[95] Chen Z G,Zhu L,Zhang T Y,et al. A novel and selective assay for the quantitative analysis of molybdenum(VI)at nanogram level by resonance light scattering quenching technique[J]. Spectrochim Acta A,2008,70(2):290-296.
[96] Yang Q L,Lu Q M,Liu Z F,et al. Resonance Rayleigh scattering spectra of ion-association nanoparticles of [Co(4-[(5-Chloro-2-pyridyl)azo]-1,3-diaminobenzene)2 ]2+-sodium dodecyl benzene sulfonate system and its analytical application[J]. Anal Chim Acta,2009,632(1):115-121.
[97]杨清玲,刘忠芳,鲁群岷,等.盐酸氯丙嗪作探针共振瑞利散射法测定环境水样中某些阴离子表面活性剂[J].高等学校化学学报,2006,27(12):2281-2284.
[98]肖锡林,蔡亮,廖力夫,等.均匀设计优化共振瑞利散射法测定水中阴离子表面活性剂[J].光谱实验室,2009,26(3):459-463.
[99]田丽,韩权,杨晓慧,等.中性红与透明质酸钠相互作用的共振瑞利散射光谱及其分析应用[J].分析试验室,2011,30(1):90-93.
[100] Luo H Q,Li N B,Liu S P. Resonance Rayleigh scattering study of interaction of hyaluronic acid with ethyl violet dye and its analytical application[J]. Biosens Bioelectron, 2006,21(7):1186-1194.
[101] Hu Y,Fu X,Chen X D,et al. Association behaviors between carboxymethyl cellulose and polylactic acid revealed by resonance light scattering spectra[J]. Polym. Bull,2009,62(4):549-559.
[102]国家药典委员会.中华人民共和国药典(二部)[S]. 2010年版.北京:中国医药科技出版社,2010:1240.
[103]刘绍璞,孔玲,刘忠芳.乙基紫共振瑞利散射法测定羧甲基纤维素钠[J].西南师范大学学报,2006,31(1):1-7.
[104]孔玲,刘绍璞,刘忠芳.硫酸耐尔蓝共振光散射法测定羧甲基纤维素钠[J].应用化学,2006,23(7):708-713.
[105]杨季冬,邓世星,周尚.血清白蛋白-氯化铜-壳聚糖体系共振瑞利散射法测定壳聚糖[J].分析化学,2007,35(11):1619-1624.
[106] Chen Z G,Zhu L,Song T H,et al. Determination of dextrin based on its self- aggregation by resonance light scattering technique[J]. Anal Chim Acta,2009,635(2):202-206.
[107] Peng J J,Liu S P,Wang L,et al. Studying the interaction between CdTe quantum dots and Nile blue by absorption,fluorescence and resonance Rayleigh scattering spectra[J]. Spectrochim Acta A,2010,75(5):1571-1576.
[108] Yang J,Chen X Y,Fu R W,et al. Phase separation in polystyrene/poly (vinyl methyl ether) blend re vealed by two-dimensional correlation resonance light scattering spectros- copy[J]. Polymer Testing,2009,28(4):456-460.
[109] Xiao J B,Chen J W,Ren F L,et al. Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of methicillin-resistant Staphylococcus aureus by resonance light scattering[J]. Anal Chim Acta,2007,589(2): 186-191.
[110] Ma Q,Yu W,Su X G. Detection of Newcastle disease virus with quantum dots-resonance light scattering system[J]. Talanta,2010,82(1):51-55.
[111] Qi L,Han Z Q,Chen Y. Incorporation of flow injection analysis or capillary electrophoresis with resonance Rayleigh scattering detection for inorganic ion analysis[J]. J Chromatogr A,2006,1110(1/2):235-239.
[112]麻文胜,王素梅,蒋治良.氰戊菊酯的免疫共振散射光谱分析[J].光谱学与光谱分析,2009,29(1):214-216.
[113] R.C.罗,P.J.舍斯基,P.J.韦勒编,郑俊民主译.药用辅料手册(第四版)[M].北京:化学工业出版社,2004:107-111.
[114]国家药典委员会.中华人民共和国药典(二部)[S]. 2010年版.北京:中国医药科技出版社,2010:1191.
[115]王艳华,杨胜科,王文科,等. CTMAB紫外分光光度法测定钻井泥浆中羧甲基纤维素[J].分析试验室,2009,28(5):28-32.
[116] Takano S,Tsuda T,Yamanaka M. Automated Determination of Builders in Detergents.ⅡDetermination of Sodium Carboxy Methylcellulose on Basis of Anthrone Method[J]. Yukagaku,1977,26(5):287-290.
[1]卢毅,张彤,陶建生.卡波姆在药剂学中的应用[J].中国医药工业杂志,2007,38(6):457-460.
[2] R.C.罗,P.J.舍斯基,P.J.韦勒编,郑俊民主译.药用辅料手册(第四版)[M].北京:化学工业出版社,2004:107-111.
[3]薛铁中,华慢,袁立新.卡波树脂在化妆品中的应用[J].日用化学品科学,2009, 32(11):39-42.
[4]刘义,高俊.化妆品用增稠剂[J].日用化学工业,2003,33(1):44-48.
[5] Gomez C,Derakhshandeh B,Hatzikiriakos S G,et al. Carbopol as a model fluid for studying mixing of pulp fibre suspensions[J]. Chem Eng Sci,2010,65(3):1288-1295.
[6]薛小燕,周颖,刘俊杰,等.胰岛素口腔黏附片的制备及体外药学特性[J].中国药科大学学报,2010,41(6):529-534.
[7]邾枝花,刘丽,季婷婷.芙蓉膏凝胶剂制备工艺[J].中国医院药学杂志,2010,30(8):704-705.
[8]刘阳,张志荣,韩静,等. 6-巯基嘌呤结肠黏附缓释微丸的处方筛选及体外释放的研究[J].中国新药杂志,2008,17(17):1515-1518.
[9] Bron A J,Mangat H,Quinlan M,et al. Polyacrylic acid gel in patients with dry eyes: a randomized comparison with polyvinyl alcohol [J]. Eur J Ophthalmol,1998,8(2):81.
[10]宁德俄,常翠,杨宏图,等.滞留型复方氧氟沙星滴眼液的制备及质量控制[J].药学实践杂志,2004,22(5):287-289.
[11] Ceulemans J,Ludwig A. Optimisation of carbomer viscous eye drops:an in vitro experimental design approach using rheological techniques[J]. Eur J Pharm Biopharm, 2002,54(1):41-50.
[12]刘荣,冯端浩,邓夕军,等.卡波姆助悬氢氧化铝凝胶的研制[J].中国药学杂志, 1998,33(3):187.
[13] Berney B M,Deasy P B. Evaluation of cabopol 934 as a suspending agent for sulphaoimidine suspensions[J]. Int J Pharm,1979,3(2/3):73-80.
[14]刘文静,魏玉辉,宋玉琴,等.尼古丁缓释贴剂的制备及体外透皮释药评价[J].中国新药杂志,2009,18(8):755-759.
[15]王小平,何晓晖,涂国卿.锡类缓释贴膜的制备工艺研究[J].中成药,2009,31(4):621-623.
[16]李馨儒,雷耀龙,沈传勇,等.阿昔洛韦眼用pH敏感原位凝胶剂的制备和评价[J].中国新药杂志,2009,18(24):2360-2364.
[17]周绚,傅超美,何瑶,等.不同骨架材料控制三七总皂苷多成分体外均衡释放的特征[J].药学学报,2010,45(4):505-509.
[18]郑斯骥,张琦.格列吡嗪缓释片的研制[J].中国医药工业杂志,2010,41(9): 670-673.
[19] Park S H,Chun M K,Choi H K. Preparation of an extended-release matrix tablet using chitosan/Carbopol interpolymer complex [J]. Int J Pharm,2008,347(1/2):39-44.
[20] Li S H,Lin S S,Daggy B P,et al. Effect of HPMC and Carbopol on the release and floating properties of Gastric Floating Drug Delivery System using factorial design[J]. Int J Pharm,2003,253(1/2):13-22.
[21] Craig D Q.M.,Tamburic S,Buckton G,et al. An investigation into the structure and properties of Carbopol 934 gels using dielectric spectroscopy and oscillatory rheometry[J]. J Control Release,1994,30(3):213-223.
[22] Petrovic1,Cvetkovicl N,Ibric S,et al. Application of Mixture Experimental Design in the Formulation and Optimization of Matrix Tablets Containing Carbomer and Hydroxypropylmethylcellulose[J]. Arch Pharm Res,2009,32(12):1767-1774.
[23]国家药典委员会.中华人民共和国药典(二部)[S]. 2010年版.北京:中国医药科技出版社,2010:1191.
[24]邓志宏,赵少贞,张未娟,等.立宝舒卡波姆眼用凝胶治疗干眼的临床观察[J].国际眼科杂志,2009,9(8):1540-1542.
[25] Marner K,M(?)ller P M,Dillon M,et al. Viscous carbomer eye drops in patients with dry eyes[J]. Acta Ophthalmol Scand,1996,74(3):249-252.
[26]张书然,杨季冬.共振瑞利散射光谱法对盐酸异丙嗪与盐酸氯丙嗪的同时测定[J].分析测试学报,2009,28(12):1362-1367.
[27] Chen Z G,Zhu L,Song T H,et al. Determination of dextrin based on its self-aggregation by resonance light scattering technique[J]. Anal Chim Acta,2009,635(2):202-206.
[28]王彦,王子娟.卡波姆在药剂学中的应用[J].中国药事,2005,19(6):361-365.
[29]苏杰,张钧寿,吴葆金,等.卡波姆—新型药物辅料[J].中国药学杂志,2000,35(9):579-581.
[1]聂华荣,柳明珠,陈振斌.羧甲基纤维素水凝胶生物降解动力学研究[J].物理化学学报,2004,20(4):386-390.
[2] R.C.罗,P.J.舍斯基,P.J.韦勒编,郑俊民主译.药用辅料手册(第四版)[M].北京:化学工业出版社,2004:117-120.
[3] Kawashima Y,Iwamoto T,Niwa T,et al. Preparation and characterization of a new controlled release ibuprofen suspension for improving suspendability[J]. Int J Pharm, 1991,75(1):25-36.
[4] Falamaki C,Naimi M,Aghaie A. Dip-coating technique for the manufacture of alumina microfilters using PVA and Na-CMC as binders:a comparative study[J]. J Eur Ceram Soc,2006,26(6):949-956.
[5] Lucy S.C.Wan,Kanneganti P.P.Prasad. Uptake of water into tablets with low-substituted carboxymethyl cellulose sodium as disintegrant[J]. Int J Pharm,1989,55(2/3):115-121.
[6] Oza K P,Frank S G. Microcrystalline cellulose stabilized emulsions[J]. J Disper Sci, 1986,7(5):543-561.
[7]李明秋.沐浴露之发展和配方特点[J].中国洗涤用品工业,2004,(2):43-44.
[8] Shams K,Mirmohammadi S J. Preparation of 5A zeolite monolith granular extrudates using kaolin:Investigation of the effect of binder on sieving/adsorption properties using a mixture of linear and branched paraffin hydrocarbons [J]. Micropor Mesopor Mat,2007, 106(1/3):268-277.
[9]李华,牛生洋,王华,等.羧甲基纤维素钠对葡萄酒酒石稳定的研究[J].中国食品添加剂,2003(6):35-38.
[10] Sato T,Nalepa D E. Shear Degradation of Cellulose Derivatives[J]. J Appl Polym Sci, 1978,22(3):856-867.
[11] Eyler R W,Klug E D,Floyd. Diephuis.Determination of Degree of Substitution of Sodium Carboxymethylcellulose[J]. Anal Chem,1947,19(1):24–27.
[12]张燕兴,叶君,熊犍.红外光谱测定羧甲基纤维素钠的羧基含量[J].造纸科学与技术,2010,29(1):71-77.
[13]胡勇,扶雄,陈旭东.羧甲基纤维素与聚乳酸之间相互作用的光谱法研究[J].中山大学学报,2010,49(4):67-54.
[14]国家药典委员会.中华人民共和国药典(二部)[S]. 2010年版.北京:中国医药科技出版社,2010:1240.
[15] Takano S,Tsuda T,Yamanaka M. Automated Determination of Builders in DetergentsⅡDetermination of Sodium Carboxy Methylcellulose on Basis of Anthrone Method[J]. Yukagaku,1977,26(5):287-290.
[16]陆海燕,宋美,史卫东,等.蒽酮光度法测定羧甲基纤维素钠[J].天津轻工业学院学报,1996,2:86-88.
[17] Koh G L,Tucker I G. Variability in the phenol-sulphuric acid assay for sodium carboxymethylcellulose[J]. Int J Pharm,1986,34:183-184.
[18]王艳华,杨胜科,王文科,等. CTMAB紫外分光光度法测定钻井泥浆中羧甲基纤维素[J].分析试验室,2009,28(5):28-32.
[19] Yomota C,Toyoda M,Ito Y,et al. An Improved Determination Method for Sodium Carboxymethylcellulose in Various Foods [J]. Shokuhin Eiseiguku Zasshi,1984, 25(6):505-511.
[20] Hall Randal A,Wooten J B. Quantitative Analysis of Cellulose in Tobacco by 13C CPMAS NMR[J]. J Agric Food Chem,1998,46(4):1423–1427.
[21]刘绍璞,陈飒,胡小莉,等.羧甲基纤维素对某些碱性吖啶染料的褪色作用及其分析应用[J].分析化学,2005,33(5):639-642.
[22] Liu S P,Chen S,Liu Z F,et al. Resonance Rayleigh scattering spectra of interaction of sodium carboxymethylcellulose with cationic acridine dyes and their analytical applications [J]. Anal Chim Acta,2005,535(1/2):169–175.
[23]刘绍璞,孔玲,刘忠芳.乙基紫共振瑞利散射法测定羧甲基纤维素钠[J].西南师范大学学报,2006,31(1):1-7.
[24]孔玲,刘绍璞,刘忠芳.硫酸耐尔蓝共振光散射法测定羧甲基纤维素钠[J].应用化学,2006,23(7):708-713.
[25] Chen Z G,Zhu L,Song T H,et al. Determination of dextrin based on its self- aggregation by resonance light scattering technique[J]. Anal Chim Acta,2009,635(2):202–206.
[26]张书然,杨季冬.共振瑞利散射光谱法对盐酸异丙嗪与盐酸氯丙嗪的同时测定[J].分析测试学报,2009,28(12):1362-1367.