分泌型和非分泌型烟草叶面腺毛对镉胁迫的应答反应研究
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摘要
腺毛作为植物表皮细胞的特化结构,在植物与环境互作中起重要作用。为探究不同类型腺毛在镉(Cd)胁迫中所发挥的作用,以非分泌型烟草品种T.I.1112和分泌型烟草品种T.I.1068为材料,研究二者对Cd胁迫的耐受性差异、腺毛形态和叶片中Cd积累的动态变化规律。结果发现,分泌型烟草品种T.I.1068对Cd的耐受性明显高于非分泌型烟草品种T.I.1112。显微观察发现,Cd胁迫下分泌型烟草品种和非分泌型烟草品种叶面腺毛密度都明显增加,但腺毛类型保持不变。电感耦合等离子体发射光谱(ICP-OES)检测发现,烟叶中的总Cd含量随处理时间延长而逐渐增加,分泌型烟草品种和非分泌型烟草品种叶片的总Cd含量基本一致,但二者叶表面的Cd含量存在显著差异,分泌型烟草叶面的Cd含量远远大于非分泌型烟草。表明分泌型腺毛能排出烟叶中的Cd,降低烟叶中的镉含量,对提高烟草对Cd胁迫的耐受性具有重要作用,结果为烟草品种改良、提高烟草制品安全性及土壤修复等方面提供理论基础。
As a specialized structure of plant epidermal cells,trichomes play an important role in plant-environment interaction. In present work,non-glandular tobacco variety T.I.1112 and glandular tobacco variety T.I.1068 were used to study the difference between glandular and non-glandular trichomes in response to cadmium( Cd) stress. By comparing the differential performance between T.I.1112 and T. I.1068 under Cd stress,it was found that T. I.1068 enhanced tolerance to Cd stress. The results of inductively coupled plasma optical emission spectrometry( ICP-OES)showed that the total Cd content in tobacco leaves gradually increased along with Cd treatment time,with the final Cd content in T.I.1112 and T.I.1068 showed no obvious difference,but the secreted Cd on leaf surface differed greatly,with much higher Cd content on leaf surface of glandular tobacco than non-glandular tobacco. These results indicated that glandular trichomes play more important role in improving tobacco tolerance to Cd by excluding toxic Cd outside the plant. Above results provided references for further work on tobacco breeding,cigarette safety improvement and soil remediation.
As a specialized structure of plant epidermal cells,trichomes play an important role in plant-environment interaction. In present work,non-glandular tobacco variety T.I.1112 and glandular tobacco variety T.I.1068 were used to study the difference between glandular and non-glandular trichomes in response to cadmium( Cd) stress. By comparing the differential performance between T.I.1112 and T. I.1068 under Cd stress,it was found that T. I.1068 enhanced tolerance to Cd stress. The results of inductively coupled plasma optical emission spectrometry( ICP-OES)showed that the total Cd content in tobacco leaves gradually increased along with Cd treatment time,with the final Cd content in T.I.1112 and T.I.1068 showed no obvious difference,but the secreted Cd on leaf surface differed greatly,with much higher Cd content on leaf surface of glandular tobacco than non-glandular tobacco. These results indicated that glandular trichomes play more important role in improving tobacco tolerance to Cd by excluding toxic Cd outside the plant. Above results provided references for further work on tobacco breeding,cigarette safety improvement and soil remediation.
引文
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[10]Huchelmann A,Boutry M,Hachez C.Plant glandular trichomes:Natural cell factories of high biotechnological interest[J].Plant Physiol.,2017,175(1):6-22.
[11]Choi Y E,Harada E,Wada M,et al..Detoxification of cadmium in tobacco plants:Formation and active excretion of crystals containing cadmium and calcium through trichomes[J].Planta,2001,213(1):45-50.
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[13]Choi Y E,Harada E.Roles of calcium and cadmium on Cdcontaining intra-and extracellular formation of Ca crystals in tobacco[J].J.Plant Biol.,2005,48(1):113-119.
[14]娄亚楠,王召军,杨欣玲,等.茉莉酸甲酯对烟草分泌型和非分泌型腺毛形态发生的影响[J].中国烟草学报,2018,24(02):24-29.Lou Y N,Wang Z J,Yang X L,et al..Effects of methyl jasmonic acid on morphogenesis of tobacco glandular and nonglandular trichomes[J].Acta Tab.Sin.,2018,24(2):24-29.
[15]Lux A,Vaculík M,Martinka M,et al..Cadmium induces hypodermal periderm formation in the roots of the monocotyledonous medicinal plant Merwilla plumbea[J].Ann.Bot.,2011,107(2):285-292.
[16]Souguir D,Ferjani E,Ledoigt G,et al..Sequential effects of cadmium on genotoxicity and lipoperoxidation in Vicia faba roots[J].Ecotoxicology,2011,20(2):329-336.
[17]袁祖丽,马新明,韩锦峰,等.镉污染对烟草叶片超微结构及部分元素含量的影响[J].生态学报,2005,25(11):2919-2927.Yuan Z L,Ma X M,Han J F,et al..Effect of Cd contamination on ultramicroscopic structure and some elements content of tobacco leaves[J].Acta Ecol.Sin.,2005,25(11):2919-2927.
[18]刘义新,陶涌,孟丽华,等.烤烟品种K326和云烟87对镉胁迫的生理响应及抗性差异[J].中国烟草科学,2008,29(04):1-5.Liu Y X,Tao Y,Meng L H,et al..Physiological response and resistance of K326 and Yunyan 87 to cadmium[J].Chin.Tob.Sci.,2008,29(4):1-5.
[19]Yadav R K,Sangwan R S,Sabir F,et al..Effect of prolonged water stress on specialized secondary metabolites,peltate glandular trichomes,and pathway gene expression in Artemisia annua L.[J].Plant Physiol.Biochem.,2014,74:70-83.
[2]杜丽娜,余若祯,王海燕,等.重金属镉污染及其毒性研究进展[J].环境与健康杂志,2013,30(02):167-174.Du L N,Yu R Z,Wang H Y,et al..Pollution and toxicity of cadmium:A review of recent studies[J].J.Environ.Health,2013,30(02):167-174.
[3]Lugon-Moulin N,Zhang M,Gadani F,et al..Critical review of the science and options for reducing cadmium in tobacco(Nicotiana Tabacum L.)and other plants[J].Adv.Agron.,2004,83:111-180.
[4]曹晨亮,马义兵,李菊梅,等.烟草镉的健康风险评价及消减技术研究进展[J].应用生态学报,2015,26(04):1279-1288.Cao C L,Ma Y B,Li J M,et al..Tobacco cadmium health risk assessment and reduction techniques:A review[J].Chin.J.Appl.Ecol.,2015,26(4):1279-1288.
[5]王文元,段焰青,夏建军,等.ICP-MS法测定卷烟主流烟气中的铬·镍·镉·铅[J].安徽农业科学,2014,42(1):219-220,277.Wang W Y,Duan Y Q,Xia J J,et al..Determination of chromium,nickel,cadmium and lead in mainstream cigarette smoke by ICP-MS[J].J.Anhui Agric.Sci.,2014,42(1):219-220,277.
[6]Weeks W W,Sisson V A,Chaplin J F.Differences in aroma,chemistry,solubilities,and smoking quality of cured flue-cured tobaccos with aglandular and glandular trichomes[J].J.Agric.Food Chem.,1992,40(10):1911-1916.
[7]冀浩,李雪君,赵永振,等.浸提叶面分泌物对烤烟品质的影响[J].中国烟草科学,2008,29(02):13-17.Ji H,Li X J,Zhao Y Z,et al..Effect of leaf surface exudate extraction on flue-cured tobacco quality[J].Chin.Tob.Sci.,2008,29(2):13-17.
[8]Shepherd R W,Bass W T,Houtz R L,et al..Phylloplanins of tobacco are defensive proteins deployed on aerial surfaces by short glandular trichomes[J].Plant Cell,2005,17(6):1851.
[9]Wang E,Wang R,De Parasis J,et al..Suppression of a P450hydroxylase gene in plant trichome glands enhances naturalproduct-based aphid resistance[J].Nat.Biotechnol.,2001,19(4):371-374.
[10]Huchelmann A,Boutry M,Hachez C.Plant glandular trichomes:Natural cell factories of high biotechnological interest[J].Plant Physiol.,2017,175(1):6-22.
[11]Choi Y E,Harada E,Wada M,et al..Detoxification of cadmium in tobacco plants:Formation and active excretion of crystals containing cadmium and calcium through trichomes[J].Planta,2001,213(1):45-50.
[12]Choi Y E,Harada E,Kim G H,et al..Distribution of elements on tobacco trichomes and leaves under cadmium and sodium stresses[J].J.Plant Biol.,2004,47(2):75-82.
[13]Choi Y E,Harada E.Roles of calcium and cadmium on Cdcontaining intra-and extracellular formation of Ca crystals in tobacco[J].J.Plant Biol.,2005,48(1):113-119.
[14]娄亚楠,王召军,杨欣玲,等.茉莉酸甲酯对烟草分泌型和非分泌型腺毛形态发生的影响[J].中国烟草学报,2018,24(02):24-29.Lou Y N,Wang Z J,Yang X L,et al..Effects of methyl jasmonic acid on morphogenesis of tobacco glandular and nonglandular trichomes[J].Acta Tab.Sin.,2018,24(2):24-29.
[15]Lux A,Vaculík M,Martinka M,et al..Cadmium induces hypodermal periderm formation in the roots of the monocotyledonous medicinal plant Merwilla plumbea[J].Ann.Bot.,2011,107(2):285-292.
[16]Souguir D,Ferjani E,Ledoigt G,et al..Sequential effects of cadmium on genotoxicity and lipoperoxidation in Vicia faba roots[J].Ecotoxicology,2011,20(2):329-336.
[17]袁祖丽,马新明,韩锦峰,等.镉污染对烟草叶片超微结构及部分元素含量的影响[J].生态学报,2005,25(11):2919-2927.Yuan Z L,Ma X M,Han J F,et al..Effect of Cd contamination on ultramicroscopic structure and some elements content of tobacco leaves[J].Acta Ecol.Sin.,2005,25(11):2919-2927.
[18]刘义新,陶涌,孟丽华,等.烤烟品种K326和云烟87对镉胁迫的生理响应及抗性差异[J].中国烟草科学,2008,29(04):1-5.Liu Y X,Tao Y,Meng L H,et al..Physiological response and resistance of K326 and Yunyan 87 to cadmium[J].Chin.Tob.Sci.,2008,29(4):1-5.
[19]Yadav R K,Sangwan R S,Sabir F,et al..Effect of prolonged water stress on specialized secondary metabolites,peltate glandular trichomes,and pathway gene expression in Artemisia annua L.[J].Plant Physiol.Biochem.,2014,74:70-83.