双斑长跗萤叶甲对棉花和玉米七种挥发物的EAG及行为反应
|
摘要
【目的】为筛选适合配制双斑长跗萤叶甲Monolepta hieroglyphica(Motschulsky)引诱剂的有效成分。【方法】本试验采用昆虫触角电位反应仪和"Y"型嗅觉仪测定了双斑长跗萤叶甲雌、雄成虫对棉花和玉米7种挥发物的电生理反应和行为反应。【结果】双斑长跗萤叶甲雌、雄成虫均对10μg·mL~(-1)和1μg·mL~(-1)的γ-萜品烯、10μg·mL~(-1)的D-柠檬烯、10μg·mL~(-1)的β-紫罗酮的EAG反应较明显,对10μg·mL~(-1)和10~(-2)μg·mL~(-1)的α-石竹烯和10μg·mL~(-1)、1μg·mL~(-1)和10-2μg·mL~(-1)的氧化石竹烯的反应次之,对十二烷、十三烷的反应较不明显。t-检验表明,雌、雄成虫对挥发性物部分浓度的EAG反应存在显著差异。行为反应测定结果表明,在10μg·mL~(-1)刺激剂量条件下,β-紫罗酮对雌虫表现出明显的引诱作用,γ-萜品烯和氧化石竹烯对其有显著的驱避作用;γ-萜品烯和D-柠檬烯对雄虫具有显著的引诱作用,β-紫罗酮对其具有显著的驱避作用。【结论】该研究结果可为双斑长跗萤叶甲田间引诱剂的研发提供借鉴。
[Objectives] To identify chemical lures for trapping male and female Monolepta hieroglyphica(Motschulsky). [Methods] Electroantennography and a Y-shaped olfactometer were used to test the electroantennography(EAG) and behavioral responses of M. hieroglyphica to selected cotton and corn volatiles. [Results] The results show that the most significant EAG responses of M. hieroglyphica males and females were elicited by 10 μg·mL~(-1) and 1 μg·mL~(-1) of γ-terpinene, 10 μg·mL~(-1) of D-limonene, and 10 μg·mL~(-1) of β-Ionone, followed by 10 μg·mL~(-1) and 10-2 μg·mL~(-1) of α-caryophyllene, and 10 μg·mL~(-1), 1 μg·mL~(-1) and 10-2 μg·mL~(-1) of caryophyllene oxide. Both males and females had a lower response to odecane and tridecane. A Student's t-test showed significant differences in the EAG responses of males and females to partial concentrations of volatiles. The results of Y-shaped olfactometer bioassays indicate that females were attracted by 10 μg/m L of β-Ionone, but were repelled by γ-terpinene and caryophyllene oxide. Males were attracted by γ-terpinene and D-limonene but repulsed by β-Ionone. [Conclusion] These results can provide a basis for developing attractants for M. hieroglyphica in the field.
[Objectives] To identify chemical lures for trapping male and female Monolepta hieroglyphica(Motschulsky). [Methods] Electroantennography and a Y-shaped olfactometer were used to test the electroantennography(EAG) and behavioral responses of M. hieroglyphica to selected cotton and corn volatiles. [Results] The results show that the most significant EAG responses of M. hieroglyphica males and females were elicited by 10 μg·mL~(-1) and 1 μg·mL~(-1) of γ-terpinene, 10 μg·mL~(-1) of D-limonene, and 10 μg·mL~(-1) of β-Ionone, followed by 10 μg·mL~(-1) and 10-2 μg·mL~(-1) of α-caryophyllene, and 10 μg·mL~(-1), 1 μg·mL~(-1) and 10-2 μg·mL~(-1) of caryophyllene oxide. Both males and females had a lower response to odecane and tridecane. A Student's t-test showed significant differences in the EAG responses of males and females to partial concentrations of volatiles. The results of Y-shaped olfactometer bioassays indicate that females were attracted by 10 μg/m L of β-Ionone, but were repelled by γ-terpinene and caryophyllene oxide. Males were attracted by γ-terpinene and D-limonene but repulsed by β-Ionone. [Conclusion] These results can provide a basis for developing attractants for M. hieroglyphica in the field.
引文
Benelli G,Pavela R,Maggi F,Petrelli R,Nicoletti M,2017.Commentary:making green pesticides greener?The potential of plant products for nanosynthesis and pest control.Journal of Cluster Science,28(1):1-8.
Bernays EA,Chapman RF,1994.Host-Plant Selection by Phytophagous Insects.New York:Chapman&Hall.1-312.
Blight MM,Pickett JA,Wadhams LJ,Woodcock CM,1995.Antennal perception of oilseed rape,Brassica napus,(Brassicaceae)by the cabbage seedpod weevil Ceutorhynchus assimilis(Coleoptera,Curculionidae).Journal of Chemical Ecology,21(11):1649-1664.
Byers JA,Lanne BS,L?fqvist J,Schlyter F,Bergstr?m G,1985.Olfactory recognition of host-tree suscep-tibility by pine shoot beetles.Naturwissenschaften,72(6):324-326.
Cai PY,Mao SM,Zhang HY,Sun HZ,2014.Current progress in research of botanical insecticides in China and worldwide.Agrochemicals,53(8):547-551.[蔡璞瑛,毛绍名,章怀云,孙汉洲,2014.植物源杀虫剂国内外研究进展.农药,53(8):547-551.]
Chen GH,Yin W,Li Q,Hu HY,2016.Research progress on Monolepta hieroglyphica(Motschulsky).China Plant Protection,36(10):19-26.[陈光辉,尹弯,李勤,胡红英.2016.双斑长跗萤叶甲研究进展.中国植保导刊,36(10):19-26.]
Chen J,Zhang JP,Zhang JH,Yu HF,Li GW,2007.Food preference of Monolepta hieroglyphica.Chinese Bulletin of Entomology,44(3):357-360.[陈静,张建萍,张建华,喻峰华,李广伟,2007.双斑长跗萤叶甲的嗜食性.昆虫知识,44(3):357-360.]
Dev V,Whaley WH,Bailey SR,Chea E,Dimaano JG,Jogani DK,Eggett D,2010.Essential oil composition of nine Apiaceae species from western United States that attract the female Indra swallowtail butterfly(Papilio indra).Biochemical Systematics and Ecology,38(4):538-547.
Dickens JC,1989.Green leaf volatiles enhance aggregation pheromone of boll weevil,Anthonomus grandis.Entomologia Experimentalis et Applicata,52(3):191-203.
Fan H,Jin YJ,Li JQ,Chen HJ,2004.Advances on plant volatile semiochemicals attracting herbivorous insects.Journal of Beijing Forestry University,26(3):76-81.[樊慧,金幼菊,李继泉,陈华君,2004.引诱植食性昆虫的植物挥发性信息化合物的研究进展.北京林业大学学报,26(3):76-81.]
Haralambos EK,Georgia P,Athanasios A,Nicolaos S,Nikolaos R,2005.Composition and insect attracting activity of the essential oil of Rosmarinus officinalis.Journal of Chemical Ecology,31(1):111-122.
Li GW,Chen XL,2010.Studies on biological characteristic and population dynamics of Monolepta hieroglyphica in cotton in Xinjiang.China Plant Protection,30(6):8-10.[李广伟,陈秀琳,2010.新疆棉区双斑长跗萤叶甲生活习性及消长动态调查研究.中国植保导刊,30(6):8-10.]
Li JX,2008.The chemosensory mechanism of beet armyworm Spodoptera exigua(Hübner).Master dissertation.Chongqing:Southwest University.[李建勋,2008.甜菜夜蛾Spodoptera exigua(Hübner)化学感受基础的研究.硕士学位论文.重庆:西南大学.]
Li Q,2013.Electrophysiological responses to corn volatiles and antennal ultra-structure of Conogethes punctiferalis(Guenée).Master dissertation.Baoding:Hebei Agricultural University.[李倩,2013.桃蛀螟对玉米挥发性物质的电生理反应及触角超微结构观察.硕士学位论文.保定:河北农业大学.]
Liu H,Chi DF,Chen HY,Yu J,Li XC,2013.EAG and behavioral responses of Monolepta hieroglyphica(Motschulsky)to several volatile compounds.Forest Research,26(4):488-493.[刘航,迟德富,陈海一,宇佳,李晓灿,2013.双斑长跗萤叶甲对几种植物挥发物的触角电位和行为反应.林业科学研究,26(4):488-493.]
Liu X,Tian G,Wang FY,Li HX,Li ZH,Wang XY,Wang ZH,Wang GL,Wang YW,2016.Population dynamics of Monolepta hieroglyphica(Motschulsky)in millet field.Chinese Agricultural Science Bulletin,32(21):177-180.[刘鑫,田岗,王枫叶,李会霞,李志华,王晓宇,王振华,王国梁,王玉文,2016.谷田中双斑长跗萤叶甲种群动态初步研究.中国农学通报,32(21):177-180.]
Mo SS,Zhao DX,Chen Q,2006.Advances on relationship between plant volatiles and insect behavior.Chinses Journal of Tropical Agriculture,26(6):84-89.[莫圣书,赵冬香,陈青,2006.植物挥发物与昆虫行为关系研究进展.热带农业科学,26(6):84-89.]
ParéPW,Tumlinson JH,1999.Plant volatiles as a defense against insect herbivores.Plant Physiology,121(2):325-332.
Pettersson J,Karunaratne S,Ahmed E,1998.The cowpea aphid,shape Aphis craccivora,host plant odours and pheromones.Entomologia Experimentalis et Applicata,88(2):177-184.
Pichersky E,Gershenzon J,2002.The formation and function of plant volatiles:perfumes for pollinator attraction and defense.Current Opinion in Plant Biology,5(3):237-243.
Raguso RA,Light DM,Pickersky E,1996.Electroantennogram responses of Hyles lineata(Sphingidae:Lepidoptera)to volatile compounds from Clarkia breweri(Onagraceae)and other moth-pollinated flowers.Journal of Chemical Ecology,22(10):1735-1766.
Shao RZ,Liu XA,Sun L,Hu LF,2017.Progresses in botanical pesticides in China.Hubei Agricultural Sciences,56(8):1401-1404.[邵仁志,刘小安,孙兰,胡利锋,2017.中国植物源农药的研究进展.湖北农业科学,56(8):1401-1405.]
Showler AT,2001.Spodoptera exigua oviposition and larval feeding preferences for pigweed,Amaranthus hybridus,over squaring cotton,Gossypium hirsutum,and a comparison of free amino
acids in each host plant.Journal of Chemical Ecology,27:2013-2028.
Sun YQ,2008.Sensilla and behavioral responses of adults of Semanotus bifasciatus(Coleoptera:Cerambycidae)to volatile compounds od Platycladus orientalis.Master dissertation.Beijing:Beijing Forestry University.[孙月琴,2008.双条杉天牛成虫的感受器及对侧柏挥发物的行为反应.硕士学位论文.北京:北京林业大学.]
Tian J,Cui J,Wu L,Xu W,Chen BC,Shi SS,2014.Screening of pesticides for controlling Monolepta hieroglyphica(Motschulsky).Agrochemicals,53(10):767-770.[田径,崔娟,吴磊,徐伟,陈宝存,史树森,2014.双斑长跗萤叶甲Monolepta hieroglyphica(Motschulsky)的药剂防治试验.农药,53(10):767-770.]
Tichenor LH,Seigler DS,1980.Electroanlennogram and oviposition responses of Manduca sexta to volatile components of tobacco and tomato.Journal of Insect Physiology,26(5):309-314.
Wang PX,2015.EAG response of Asian longhorn beetle Anoplophora glabripennis(Coleoptera:Cerambycidae)to volatiles of Hickory.Master dissertation.Hangzhou:Zhejiang A&FUniversity.[王佩星,2015.光肩星天牛对山核桃挥发物的触角电生理反应.硕士学位论文.杭州:浙江农林大学.]
Wang RL,Yang W,Yang ZZ,Chen XP,Yang CP,Li Q,Li F,Chen CM,2011.Electroantennographic and behavioral responses of Dendroctonus armandi(Coleoptera:Ipidae)to host volatiles.Chinese Journal of Ecology,30(4):724-729.[王茹琳,杨伟,杨佐忠,陈小平,杨春平,李强,李锋,陈春茂,2011.华山松大小蠹对几种寄主挥发物组分的EAG和行为反应.生态学杂志,30(4):724-729.]
Wang YC,Li P,Chi DF,2017.Electrophysiological and laboratory behavioral responses of a leaf beetle pest of elm,Ambrostoma quadriimpressum,to selected plant volatiles and essential oils.Entomologia Experimentalis et Applicata,163(2):140-149.
Wanjiku C,Khamis FM,Teal PEA,Torto B,2014.Plant volatiles influence the african weaver ant-cashew tree mutualism.Journal of Chemical Ecology,40(11/12):1167-1175.
Yan SC,Cheng H,Yang H,Yuan HE,Zhang J,Chi DF,2006.Effects of plant volatiles on the EAG response and behavior of the grey tiger longicorn,Xylotrechus rusticus(L.)(Coleoptera:Cerambycidae).Acta Entomologica Sinica,49(5):759-767.[严善春,程红,杨慧,袁红娥,张健,迟德富,2006.青杨脊虎天牛对植物源挥发物的EAG和行为反应.昆虫学报,49(5):759-767.]
Yan XF,Liu YH,Li G,Qiang DH,Xu YP,2017.EAG and olfactory behavioral responses of Scythropus yasumatsui to seven volatiles from the Zizyphus juube.Chinese Journal of Applied Entomology,54(4):621-628.[阎雄飞,刘永华,李刚,强大宏,许育平,2017.枣飞象对枣树七种挥发物EAG和嗅觉行为反应.应用昆虫学报,54(4):621-628.]
Yu PY,Wang SS,Yang XK.1996.Economic Insect Fauba of China.Beijing:Science Press.82-196.[虞佩玉,王书永,杨星科,1996.中国经济昆虫志.北京:科学出版社.82-196.]
Zhang C,Yuan SH,Wang ZZ,He KL,Bai SX,2014.Population dynamics of Monolepta hieroglyphica(Motschulsky)in cornfields.Chinese Journal of Applied Entomology,51(3):668-675.[张聪,袁志华,王振营,何康来,白树雄,2014.双斑长跗萤叶甲在玉米田的种群消长规律.应用昆虫学报,51(3):668-675.]
Bernays EA,Chapman RF,1994.Host-Plant Selection by Phytophagous Insects.New York:Chapman&Hall.1-312.
Blight MM,Pickett JA,Wadhams LJ,Woodcock CM,1995.Antennal perception of oilseed rape,Brassica napus,(Brassicaceae)by the cabbage seedpod weevil Ceutorhynchus assimilis(Coleoptera,Curculionidae).Journal of Chemical Ecology,21(11):1649-1664.
Byers JA,Lanne BS,L?fqvist J,Schlyter F,Bergstr?m G,1985.Olfactory recognition of host-tree suscep-tibility by pine shoot beetles.Naturwissenschaften,72(6):324-326.
Cai PY,Mao SM,Zhang HY,Sun HZ,2014.Current progress in research of botanical insecticides in China and worldwide.Agrochemicals,53(8):547-551.[蔡璞瑛,毛绍名,章怀云,孙汉洲,2014.植物源杀虫剂国内外研究进展.农药,53(8):547-551.]
Chen GH,Yin W,Li Q,Hu HY,2016.Research progress on Monolepta hieroglyphica(Motschulsky).China Plant Protection,36(10):19-26.[陈光辉,尹弯,李勤,胡红英.2016.双斑长跗萤叶甲研究进展.中国植保导刊,36(10):19-26.]
Chen J,Zhang JP,Zhang JH,Yu HF,Li GW,2007.Food preference of Monolepta hieroglyphica.Chinese Bulletin of Entomology,44(3):357-360.[陈静,张建萍,张建华,喻峰华,李广伟,2007.双斑长跗萤叶甲的嗜食性.昆虫知识,44(3):357-360.]
Dev V,Whaley WH,Bailey SR,Chea E,Dimaano JG,Jogani DK,Eggett D,2010.Essential oil composition of nine Apiaceae species from western United States that attract the female Indra swallowtail butterfly(Papilio indra).Biochemical Systematics and Ecology,38(4):538-547.
Dickens JC,1989.Green leaf volatiles enhance aggregation pheromone of boll weevil,Anthonomus grandis.Entomologia Experimentalis et Applicata,52(3):191-203.
Fan H,Jin YJ,Li JQ,Chen HJ,2004.Advances on plant volatile semiochemicals attracting herbivorous insects.Journal of Beijing Forestry University,26(3):76-81.[樊慧,金幼菊,李继泉,陈华君,2004.引诱植食性昆虫的植物挥发性信息化合物的研究进展.北京林业大学学报,26(3):76-81.]
Haralambos EK,Georgia P,Athanasios A,Nicolaos S,Nikolaos R,2005.Composition and insect attracting activity of the essential oil of Rosmarinus officinalis.Journal of Chemical Ecology,31(1):111-122.
Li GW,Chen XL,2010.Studies on biological characteristic and population dynamics of Monolepta hieroglyphica in cotton in Xinjiang.China Plant Protection,30(6):8-10.[李广伟,陈秀琳,2010.新疆棉区双斑长跗萤叶甲生活习性及消长动态调查研究.中国植保导刊,30(6):8-10.]
Li JX,2008.The chemosensory mechanism of beet armyworm Spodoptera exigua(Hübner).Master dissertation.Chongqing:Southwest University.[李建勋,2008.甜菜夜蛾Spodoptera exigua(Hübner)化学感受基础的研究.硕士学位论文.重庆:西南大学.]
Li Q,2013.Electrophysiological responses to corn volatiles and antennal ultra-structure of Conogethes punctiferalis(Guenée).Master dissertation.Baoding:Hebei Agricultural University.[李倩,2013.桃蛀螟对玉米挥发性物质的电生理反应及触角超微结构观察.硕士学位论文.保定:河北农业大学.]
Liu H,Chi DF,Chen HY,Yu J,Li XC,2013.EAG and behavioral responses of Monolepta hieroglyphica(Motschulsky)to several volatile compounds.Forest Research,26(4):488-493.[刘航,迟德富,陈海一,宇佳,李晓灿,2013.双斑长跗萤叶甲对几种植物挥发物的触角电位和行为反应.林业科学研究,26(4):488-493.]
Liu X,Tian G,Wang FY,Li HX,Li ZH,Wang XY,Wang ZH,Wang GL,Wang YW,2016.Population dynamics of Monolepta hieroglyphica(Motschulsky)in millet field.Chinese Agricultural Science Bulletin,32(21):177-180.[刘鑫,田岗,王枫叶,李会霞,李志华,王晓宇,王振华,王国梁,王玉文,2016.谷田中双斑长跗萤叶甲种群动态初步研究.中国农学通报,32(21):177-180.]
Mo SS,Zhao DX,Chen Q,2006.Advances on relationship between plant volatiles and insect behavior.Chinses Journal of Tropical Agriculture,26(6):84-89.[莫圣书,赵冬香,陈青,2006.植物挥发物与昆虫行为关系研究进展.热带农业科学,26(6):84-89.]
ParéPW,Tumlinson JH,1999.Plant volatiles as a defense against insect herbivores.Plant Physiology,121(2):325-332.
Pettersson J,Karunaratne S,Ahmed E,1998.The cowpea aphid,shape Aphis craccivora,host plant odours and pheromones.Entomologia Experimentalis et Applicata,88(2):177-184.
Pichersky E,Gershenzon J,2002.The formation and function of plant volatiles:perfumes for pollinator attraction and defense.Current Opinion in Plant Biology,5(3):237-243.
Raguso RA,Light DM,Pickersky E,1996.Electroantennogram responses of Hyles lineata(Sphingidae:Lepidoptera)to volatile compounds from Clarkia breweri(Onagraceae)and other moth-pollinated flowers.Journal of Chemical Ecology,22(10):1735-1766.
Shao RZ,Liu XA,Sun L,Hu LF,2017.Progresses in botanical pesticides in China.Hubei Agricultural Sciences,56(8):1401-1404.[邵仁志,刘小安,孙兰,胡利锋,2017.中国植物源农药的研究进展.湖北农业科学,56(8):1401-1405.]
Showler AT,2001.Spodoptera exigua oviposition and larval feeding preferences for pigweed,Amaranthus hybridus,over squaring cotton,Gossypium hirsutum,and a comparison of free amino
acids in each host plant.Journal of Chemical Ecology,27:2013-2028.
Sun YQ,2008.Sensilla and behavioral responses of adults of Semanotus bifasciatus(Coleoptera:Cerambycidae)to volatile compounds od Platycladus orientalis.Master dissertation.Beijing:Beijing Forestry University.[孙月琴,2008.双条杉天牛成虫的感受器及对侧柏挥发物的行为反应.硕士学位论文.北京:北京林业大学.]
Tian J,Cui J,Wu L,Xu W,Chen BC,Shi SS,2014.Screening of pesticides for controlling Monolepta hieroglyphica(Motschulsky).Agrochemicals,53(10):767-770.[田径,崔娟,吴磊,徐伟,陈宝存,史树森,2014.双斑长跗萤叶甲Monolepta hieroglyphica(Motschulsky)的药剂防治试验.农药,53(10):767-770.]
Tichenor LH,Seigler DS,1980.Electroanlennogram and oviposition responses of Manduca sexta to volatile components of tobacco and tomato.Journal of Insect Physiology,26(5):309-314.
Wang PX,2015.EAG response of Asian longhorn beetle Anoplophora glabripennis(Coleoptera:Cerambycidae)to volatiles of Hickory.Master dissertation.Hangzhou:Zhejiang A&FUniversity.[王佩星,2015.光肩星天牛对山核桃挥发物的触角电生理反应.硕士学位论文.杭州:浙江农林大学.]
Wang RL,Yang W,Yang ZZ,Chen XP,Yang CP,Li Q,Li F,Chen CM,2011.Electroantennographic and behavioral responses of Dendroctonus armandi(Coleoptera:Ipidae)to host volatiles.Chinese Journal of Ecology,30(4):724-729.[王茹琳,杨伟,杨佐忠,陈小平,杨春平,李强,李锋,陈春茂,2011.华山松大小蠹对几种寄主挥发物组分的EAG和行为反应.生态学杂志,30(4):724-729.]
Wang YC,Li P,Chi DF,2017.Electrophysiological and laboratory behavioral responses of a leaf beetle pest of elm,Ambrostoma quadriimpressum,to selected plant volatiles and essential oils.Entomologia Experimentalis et Applicata,163(2):140-149.
Wanjiku C,Khamis FM,Teal PEA,Torto B,2014.Plant volatiles influence the african weaver ant-cashew tree mutualism.Journal of Chemical Ecology,40(11/12):1167-1175.
Yan SC,Cheng H,Yang H,Yuan HE,Zhang J,Chi DF,2006.Effects of plant volatiles on the EAG response and behavior of the grey tiger longicorn,Xylotrechus rusticus(L.)(Coleoptera:Cerambycidae).Acta Entomologica Sinica,49(5):759-767.[严善春,程红,杨慧,袁红娥,张健,迟德富,2006.青杨脊虎天牛对植物源挥发物的EAG和行为反应.昆虫学报,49(5):759-767.]
Yan XF,Liu YH,Li G,Qiang DH,Xu YP,2017.EAG and olfactory behavioral responses of Scythropus yasumatsui to seven volatiles from the Zizyphus juube.Chinese Journal of Applied Entomology,54(4):621-628.[阎雄飞,刘永华,李刚,强大宏,许育平,2017.枣飞象对枣树七种挥发物EAG和嗅觉行为反应.应用昆虫学报,54(4):621-628.]
Yu PY,Wang SS,Yang XK.1996.Economic Insect Fauba of China.Beijing:Science Press.82-196.[虞佩玉,王书永,杨星科,1996.中国经济昆虫志.北京:科学出版社.82-196.]
Zhang C,Yuan SH,Wang ZZ,He KL,Bai SX,2014.Population dynamics of Monolepta hieroglyphica(Motschulsky)in cornfields.Chinese Journal of Applied Entomology,51(3):668-675.[张聪,袁志华,王振营,何康来,白树雄,2014.双斑长跗萤叶甲在玉米田的种群消长规律.应用昆虫学报,51(3):668-675.]