外用苯烯莫德对特应性皮炎样小鼠的治疗作用
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摘要
目的:评价外用苯烯莫德对卡泊三醇(MC903)诱导的特应性皮炎样小鼠模型的治疗作用。方法:36只BALB/c小鼠,6只作为空白对照,30只给予MC903诱导特应性皮炎后再随机分为基质组、0.5%苯烯莫德组、1.0%苯烯莫德组、0.1%糠酸莫米松组和0.1%他克莫司组,治疗前后测量皮肤厚度、经皮水丢失(TEWL)、皮损内CD4~+T细胞、胸腺基质淋巴细胞生成素(TSLP)蛋白表达。结果:0.5%和1.0%苯烯莫德乳膏组皮损评分、耳部皮肤厚度、TEWL、皮损内CD4~+T细胞及TSLP蛋白水平较基质组均显著降低(P<0.05),1.0%苯烯莫德组疗效优于0.5%苯烯莫德组和他克莫司组(P<0.05),与糠酸莫米松组差异无显著性(P>0.05)。结论:外用苯烯莫德乳膏可减轻特应性皮炎样小鼠局部炎症反应。
Objective: To assess the therapeutic effects of topical benvitimod in AD-like murine models induced by calcipotriol( MC903). Methods: Of 36 AD BALB/c murine models,6 were as normal controls and 30 were induced by MC903,and then,were divided into 5 groups: matrix group,0. 5% benvitimod group,1.0% benvitimod group,0.1% mometasone furoate group and 0.1% tacrolimus group( 6 mice in each group). The levels of lesion's thickness and TEWL,CD4~+T cells and TSLP protein in the lesions were assessed before and after treatment. Results: After treatment,the levels of dermatitis score,lesion's thickness,TEWL,CD4~+T cells and TSLP in 0.5% and 1.0% benvitimod group were lower than those in the matrix group( Ps<0.05). 1.0% benvitimod was more effective than 0.5% benvitimod and tarcrolimus. The efficacy of 1.0% benvitimod was equivalent to 0.1% mometasone furoate( P>0.05). Conclusion: Topical benvitimod can improve AD-like symptoms in BALB/c murine models.
Objective: To assess the therapeutic effects of topical benvitimod in AD-like murine models induced by calcipotriol( MC903). Methods: Of 36 AD BALB/c murine models,6 were as normal controls and 30 were induced by MC903,and then,were divided into 5 groups: matrix group,0. 5% benvitimod group,1.0% benvitimod group,0.1% mometasone furoate group and 0.1% tacrolimus group( 6 mice in each group). The levels of lesion's thickness and TEWL,CD4~+T cells and TSLP protein in the lesions were assessed before and after treatment. Results: After treatment,the levels of dermatitis score,lesion's thickness,TEWL,CD4~+T cells and TSLP in 0.5% and 1.0% benvitimod group were lower than those in the matrix group( Ps<0.05). 1.0% benvitimod was more effective than 0.5% benvitimod and tarcrolimus. The efficacy of 1.0% benvitimod was equivalent to 0.1% mometasone furoate( P>0.05). Conclusion: Topical benvitimod can improve AD-like symptoms in BALB/c murine models.
引文
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[2]Bissonnette R,Poulin Y,Zhou Y,et al.Efficacy and safety of topical WBI-1001 in patients with mild to severe atopic dermatitis:results from a 12-week,multicentre,randomized,placebo-controlled double-blind trial[J].Br J Dermatol,2012,166(4):853-860.
[3]Li M,Hener P,Zhang Z.Topical vitamin D3 and low-calcemic analogs induce thymic stromal lymphopoietin in mouse keratinocytes and trigger an atopic dermatitis[J].PANS,2006,103(31):11736-11741.
[4]Moosbrugger-Martinz V,Schmuth M,Dubrac S.A mouse model for atopic dermatitis using topical application of Vitamin D3 or of Its Analog MC903[M].Methods in Molecular Biology,Eds.BECA,Springer Science+Business Media LLC,2017:91-106,1559.
[5]Weidinger S,Novak N.Atopic dermatitis[J].Lancet,2016,387(10023):1109-1122.
[6]Silverberg JI.Public health burden and epidemiology of atopic dermatitis[J].Dermatologic Clinics,2017,35(3):283-289.
[7]李祥,张宝兰,张理涛.苯烯莫德对银屑病的治疗研究[J].中国中西医结合皮肤性病学杂志,2017,16(6):557-559.
[8]Bissonnette R,Bolduc C,Maari C,et al.Efficacy and safety of topical WBI-1001 in patients with mild to moderate psoriasis:results from a randomized double-blind placebo-controlled,phase II trial[J].J Eur Acad Dermatol Venereol,2011,26(12):1516-1521.
[9]丁晓岚,王芳,黄海艳,等.苯烯莫德对白癜风患者黑素细胞系PIG3V细胞增殖、迁移及黑素合成的影响[J].中国皮肤性病学杂志,2015,29(1):1-4.
[10]Furue M,Chiba T,Tsuji G,et al.Atopic dermatitis:immune deviation,barrier dysfunction,IgE autoreactivity and new therapies[J].Allergol Int,2017,66(3):398-403.
[11]Soumelis V,Reche PA,Kanzler H,et al.Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP[J].Nature Immunol,2002,3(7):673-680.
[12]Imai T,Baba M,Nishimura M,et al.The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4[J].Journal of Biological Chemistry,1997,272(23):15036-15042.
[13]Uysal P,Birtekocak F,Karul AB.The relationship between serum TARC,TSLP and POSTN levels and childhood atopic dermatitis[J].Clin Lab,2017,63(7):1071-1077.