高效液相色谱法测定消癌平注射液中通关藤苷A和I的含量
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摘要
目的建立高效液相色谱法(HPLC)分析消癌平注射液中通关藤苷A和I的方法并测定其含量。方法消癌平注射液经微孔滤膜过滤,用HPLC进行分析,采用Symmetry C18(5μm,250 mm×4. 6 mm)色谱柱;流动相:乙腈(A)-0. 1%磷酸水溶液(B);梯度洗脱方式(0～10 min,32%A; 10～10. 01 min,32%A～42%A; 10. 01～15 min,42%A; 15～15. 01 min,42%A～50%A; 15. 01～28 min,50%A);流速:1. 0 ml/min;检测波长:223 nm。结果通关藤苷A和I的线性范围分别为6. 60～132. 00、3. 52～70. 40μg/ml,通关藤苷A和I的平均加样回收率均> 98%,相对标准偏差均<3%。结论 HPLC准确可靠、操作简便,可用于同时测定消癌平注射液中通关藤苷A和I的含量。
Objective To establish an high performance liquid chromatography( HPLC) method for detection of Caulis Marsdeniae Tenacissimae glycoside A and I contents in Xiaoaiping Injection. Methods Xiaoaiping Injection was filtrated by microporous membrane and analyzed by HPLC. Chromatographic separation was performed on Symmetry C18( 5 μm,250 mm × 4. 6 mm) column,and the mobile phase was composed of acetonitrile( A) and-0. 1% phosphoric acid aqueous solution at a 1. 0 m L/min flow rate,and gradient elution methods were 32% A within 0-10 min,32% A-42% A within 10-10. 01 min,42% A within 10. 01-15 min,42% A-50% A within 15-15. 01 min and 50% A within 15. 01-28 min,and the detection wavelength was 223 nm. Results Calibration curves of Caulis Marsdeniae Tenacissimae glycoside A and I were linear in ranges of 6. 60-132. 00 and 3. 52-70. 40 μg/ml respectively. Average recoveries of Caulis Marsdeniae Tenacissimae glycoside A and I were all larger than 98%,and values of relative standard deviation( RSD)were all less than 3%. Conclusion HPLC method is reliable,simple and accurate which can be used for simultaneous detection of Caulis Marsdeniae Tenacissimae glycoside A and I contents in Xiaoaiping Injection.
Objective To establish an high performance liquid chromatography( HPLC) method for detection of Caulis Marsdeniae Tenacissimae glycoside A and I contents in Xiaoaiping Injection. Methods Xiaoaiping Injection was filtrated by microporous membrane and analyzed by HPLC. Chromatographic separation was performed on Symmetry C18( 5 μm,250 mm × 4. 6 mm) column,and the mobile phase was composed of acetonitrile( A) and-0. 1% phosphoric acid aqueous solution at a 1. 0 m L/min flow rate,and gradient elution methods were 32% A within 0-10 min,32% A-42% A within 10-10. 01 min,42% A within 10. 01-15 min,42% A-50% A within 15-15. 01 min and 50% A within 15. 01-28 min,and the detection wavelength was 223 nm. Results Calibration curves of Caulis Marsdeniae Tenacissimae glycoside A and I were linear in ranges of 6. 60-132. 00 and 3. 52-70. 40 μg/ml respectively. Average recoveries of Caulis Marsdeniae Tenacissimae glycoside A and I were all larger than 98%,and values of relative standard deviation( RSD)were all less than 3%. Conclusion HPLC method is reliable,simple and accurate which can be used for simultaneous detection of Caulis Marsdeniae Tenacissimae glycoside A and I contents in Xiaoaiping Injection.
引文
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[10]张阿琴,张仓,梁敬钰. HPLC法测定消癌平注射液中5种酚酸的含量[J].药学与临床研究,2011,19(6):498-500.
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[12]林耀才.乌骨藤总苷对SGC-7901、S180、P388肿瘤细胞的抑制作用研究[J].药学研究,2015,34(7):376-379,383.
[13]邹渭洪,付成效,邓丽菁.乌骨藤总苷抑制肝癌细胞增殖和AFP分泌[J].中南医学科学杂志,2013,41(4):344-346.
[14]常伟,李培培,袁平凡,等.乌骨藤总皂苷对对乙酰氨基酚诱导的小鼠肝损伤的保护作用[J].中国新药杂志,2015,24(24):2835-2838.
[15]徐凯,乔佳,韩丽,等. HPLC法测定不同产地通关藤中通关藤苷G和I的含量[J].药物分析杂志,2016,36(4):607-610.
[16]张慧,裴志东,姜欢,等. HPLC法同时测定乌骨藤中通关藤苷A和D的含量[J].药物分析杂志,2013,33(6):1037-1040.
[2]丁纪元,黄静,寇军燕,等.消癌平联合吉非替尼对耐药肺癌细胞株A549作用的实验研究[J].中华全科医学,2016,14(1):32-34.
[3]吴建钟,袁治顺,于良.消癌平联合5-氟尿嘧啶处理对肝癌细胞体外生长及癌基因表达的影响[J].海南医学院学报,2017,23(6):728-731,735.
[4]赵瑞娟,李宾.消癌平片联合TACE术治疗老年原发性肝癌的疗效和生命质量的临床观察[J].中国实用医药,2016,11(27):3-5.
[5]葛蕙心,畅继武.消癌平对人膀胱癌细胞体外生长作用的实验研究[J].临床合理用药杂志,2012,5(4B):86-87.
[6]武阳.伊立替康单药与联合消癌平治疗老年晚期胃癌的比较研究[J].中国药房,2016,27(5):657-659.
[7]白爽,李奕诺,徐鑫,等.通关藤化学成分及药理活性研究进展[J].解放军药学学报,2015,31(3):260-264.
[8]韩丽,张慧,康廷国,等.中药通关藤的研究进展[J].中华中医药学刊,2016,34(10):2329-2331.
[9]谢丽艳,徐洁,戴国梁,等. HPLC同时测定消癌平注射液中7种主要成分的含量[J].中国实验方剂学杂志,2013,19(21):86-89.
[10]张阿琴,张仓,梁敬钰. HPLC法测定消癌平注射液中5种酚酸的含量[J].药学与临床研究,2011,19(6):498-500.
[11]方奕奇,孙雪梅.通关藤化学成分及抗肿瘤作用的研究进展[J].中国生化药物杂志,2011,32(2):165-167.
[12]林耀才.乌骨藤总苷对SGC-7901、S180、P388肿瘤细胞的抑制作用研究[J].药学研究,2015,34(7):376-379,383.
[13]邹渭洪,付成效,邓丽菁.乌骨藤总苷抑制肝癌细胞增殖和AFP分泌[J].中南医学科学杂志,2013,41(4):344-346.
[14]常伟,李培培,袁平凡,等.乌骨藤总皂苷对对乙酰氨基酚诱导的小鼠肝损伤的保护作用[J].中国新药杂志,2015,24(24):2835-2838.
[15]徐凯,乔佳,韩丽,等. HPLC法测定不同产地通关藤中通关藤苷G和I的含量[J].药物分析杂志,2016,36(4):607-610.
[16]张慧,裴志东,姜欢,等. HPLC法同时测定乌骨藤中通关藤苷A和D的含量[J].药物分析杂志,2013,33(6):1037-1040.