辣椒碱单体、二氢辣椒碱单体和降二氢辣椒碱单体抗氧化效果研究
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摘要
以维生素E为对照,对辣椒碱单体、二氢辣椒碱单体、降二氢辣椒碱单体和辣椒素的还原能力进行了考察,并通过体外抗氧化实验探讨了其对超氧阴离子自由基(O_2~-·)与羟基自由基(·OH)的清除能力,研究了其抗氧化能力。结果表明,辣椒碱单体具有明显的抗氧化能力,其抗氧化效果明显好于二氢辣椒碱单体、降二氢辣椒碱单体和辣椒素;进一步比较发现,4种物质的抗氧化效果依次为:辣椒碱单体>辣椒素>二氢辣椒碱单体>降二氢辣椒碱单体。因此,以辣椒碱单体为主要原料开发天然高效的食品抗氧化剂具有重要意义。
Using vitamin E as a control,we investigated the reducing abilities of capsaicin monomer,dihydrocapsaicin monomer,nordihydrocapsaicin monomer,and capsaicinoids,discussed their scavenging abilities to superoxide anion free radical(O_2~-·)and hydroxyl free radical(·OH)by in vitro antioxidant experiments,and evaluated their antioxidant activities.The results show that capsaicin monomer has obvious antioxidant activity,and the antioxidant effect of which is superior to that of dihydrocapsaicin monomer,nordihydrocapsaicin monomer,and capsaicinoids.Through further comparison,the order of antioxidant effect is:capsaicin monomer>capsaicinoids>dihydrocapsaicin monomer>nordihydrocapsaicin monomer.Therefore,it is of great significance to develop natural and high-efficiency food antioxidants with capsaicin monomer as the main raw material.
Using vitamin E as a control,we investigated the reducing abilities of capsaicin monomer,dihydrocapsaicin monomer,nordihydrocapsaicin monomer,and capsaicinoids,discussed their scavenging abilities to superoxide anion free radical(O_2~-·)and hydroxyl free radical(·OH)by in vitro antioxidant experiments,and evaluated their antioxidant activities.The results show that capsaicin monomer has obvious antioxidant activity,and the antioxidant effect of which is superior to that of dihydrocapsaicin monomer,nordihydrocapsaicin monomer,and capsaicinoids.Through further comparison,the order of antioxidant effect is:capsaicin monomer>capsaicinoids>dihydrocapsaicin monomer>nordihydrocapsaicin monomer.Therefore,it is of great significance to develop natural and high-efficiency food antioxidants with capsaicin monomer as the main raw material.
引文
[1]景作亮,邓启良,杨南,等.辣椒碱的研究进展及应用[J].天津化工,2004,18(6):9-11.JING Z L,DENG Q L,YANG N,et al.Progress of the studies and application on the capsaicin[J].Tianjin Chemical Industry,2004,18(6):9-11.
[2]刘可春,宋广运,刘昌衡,等.辣椒碱的分离纯化及分析[J].山东科学,2000,13(1):25-28.LIU K C,SONG G Y,LIU C H,et al.Extraction,purification and analysis of capsaicinoid in capsicum[J].Shandong Science,2000,13(1):25-28.
[3] FOSTER D C,DWORKIN R H,WOOD R W.Effects of intradermal foot and forearm capsaicin injections in normal and vulvodynia-afflicted women[J].Pain,2005,117(1/2):128-136.
[4] MASON L,MOORE R A,DERRY S,et al.Systematic review of topical capsaicin for the treatment of chronic pain[J].British Medical Journal,2004,328(7446):991.
[5] WINOCUR E,GAVISH A,HALACHMI M,et al.Topical application of capsaicin for the treatment of localized pain in the temporomandibular joint area[J].Journal of Orofacial Pain,2000,14(1):31-36.
[6]李宗友.辣椒辣素对不同刺激剂诱导的脂质过氧化的调节作用[J].国外医学:中医中药分册,1994,16(3):10-12.
[7] CRUWYS S C,GARRETT N E,KIDD B L.Sensory denervation with capsaicin attenuates inflammation and nociception in arthritic rats[J].Neuroscience Letters,1995,193(3):205-207.
[8]王东,丁雪艳.辣椒碱的生产和应用[J].乙醛醋酸化工,2005(12):16-18.
[9] MARX J L.Oxygen free radicals linked to many diseases[J].Science,1987,235(4788):529-531.
[10] van CALSTEREN M R,BISSONNETTE M C,CORMIER F,et al.Spectroscopic characterization of crocetin derivatives from Crocus sativus and Gardenia jasminoides[J].Journal of Agricultural and Food Chemistry,1997,45(4):1055-1061.
[11] ROWE L A,DEGTYAREVA N,DOETSCH P W.DNA damage-induced reactive oxygen species(ROS)stress response in Saccharomyces cerevisiae[J].Free Radical Biology and Medicine,2008,45(8):1167-1177.
[12] LIU C X,LIU R C,ZHANG P,et al.Separation of capsaicin from capsaicinoids by macroporous resin adsorption chromatography[J].Journal of Separation Science,2015,38(23):4141-4145.
[13]罗春丽.辣椒精中辣椒碱类化合物的提取与纯化[D].北京:北京化工大学,2007.LUO C L.Extraction and purification of capsaicin compound from capsicol[D].Beijing:Beijing University of Chemical Technology,2007.
[14] SIDDHURAJU P,MOHAN P S,BECKER K.Studies on the antioxidant activity of Indian Laburnum(Cassia fistula L.):apreliminary assessment of crude extracts from stem bark,leaves,flowers and fruit pulp[J].Food Chemistry,2002,79(1):61-67.
[15]赵保路.氧自由基和天然抗氧化剂[M].北京:科学出版社,1999:53-67.
[16] THOLLON C,ILIOU J P,CAMBARRAT C,et al.Nature of the cardiomyocyte injury induced by lipid hydroperoxides[J].Cardiovasc Research,1995,30(5):648-655.
[2]刘可春,宋广运,刘昌衡,等.辣椒碱的分离纯化及分析[J].山东科学,2000,13(1):25-28.LIU K C,SONG G Y,LIU C H,et al.Extraction,purification and analysis of capsaicinoid in capsicum[J].Shandong Science,2000,13(1):25-28.
[3] FOSTER D C,DWORKIN R H,WOOD R W.Effects of intradermal foot and forearm capsaicin injections in normal and vulvodynia-afflicted women[J].Pain,2005,117(1/2):128-136.
[4] MASON L,MOORE R A,DERRY S,et al.Systematic review of topical capsaicin for the treatment of chronic pain[J].British Medical Journal,2004,328(7446):991.
[5] WINOCUR E,GAVISH A,HALACHMI M,et al.Topical application of capsaicin for the treatment of localized pain in the temporomandibular joint area[J].Journal of Orofacial Pain,2000,14(1):31-36.
[6]李宗友.辣椒辣素对不同刺激剂诱导的脂质过氧化的调节作用[J].国外医学:中医中药分册,1994,16(3):10-12.
[7] CRUWYS S C,GARRETT N E,KIDD B L.Sensory denervation with capsaicin attenuates inflammation and nociception in arthritic rats[J].Neuroscience Letters,1995,193(3):205-207.
[8]王东,丁雪艳.辣椒碱的生产和应用[J].乙醛醋酸化工,2005(12):16-18.
[9] MARX J L.Oxygen free radicals linked to many diseases[J].Science,1987,235(4788):529-531.
[10] van CALSTEREN M R,BISSONNETTE M C,CORMIER F,et al.Spectroscopic characterization of crocetin derivatives from Crocus sativus and Gardenia jasminoides[J].Journal of Agricultural and Food Chemistry,1997,45(4):1055-1061.
[11] ROWE L A,DEGTYAREVA N,DOETSCH P W.DNA damage-induced reactive oxygen species(ROS)stress response in Saccharomyces cerevisiae[J].Free Radical Biology and Medicine,2008,45(8):1167-1177.
[12] LIU C X,LIU R C,ZHANG P,et al.Separation of capsaicin from capsaicinoids by macroporous resin adsorption chromatography[J].Journal of Separation Science,2015,38(23):4141-4145.
[13]罗春丽.辣椒精中辣椒碱类化合物的提取与纯化[D].北京:北京化工大学,2007.LUO C L.Extraction and purification of capsaicin compound from capsicol[D].Beijing:Beijing University of Chemical Technology,2007.
[14] SIDDHURAJU P,MOHAN P S,BECKER K.Studies on the antioxidant activity of Indian Laburnum(Cassia fistula L.):apreliminary assessment of crude extracts from stem bark,leaves,flowers and fruit pulp[J].Food Chemistry,2002,79(1):61-67.
[15]赵保路.氧自由基和天然抗氧化剂[M].北京:科学出版社,1999:53-67.
[16] THOLLON C,ILIOU J P,CAMBARRAT C,et al.Nature of the cardiomyocyte injury induced by lipid hydroperoxides[J].Cardiovasc Research,1995,30(5):648-655.