响应面法优化马鞭草中总黄酮闪式提取工艺及其体外抗氧化活性
|
摘要
采用响应面法优化马鞭草中总黄酮的提取工艺。在单因素实验的基础上,以乙醇浓度(A)、料液比(B)、提取时间(C)、提取次数(D)为自变量,总黄酮含量为因变量,运用Box-Behnken设计-响应面优化马鞭草中总黄酮闪式提取工艺。并通过马鞭草总黄酮对DPPH自由基、羟自由基和超氧阴离子自由基的清除作用来评价其抗氧化活性。结果表明:马鞭草中总黄酮提取的最佳工艺条件为乙醇浓度50%,料液比为1∶35 (g/m L),提取时间为1.5 min,提取次数为2次。在此条件下,总黄酮含量达到(8.282±0.003) mg/g,与模型预测值8.280 mg/g相近。重复性试验结果表明,此方法稳定可靠,总黄酮得率高,适于马鞭草中总黄酮的提取。体外抗氧化活性实验表明,当马鞭草中总黄酮浓度为60μg/m L时,其对DPPH、羟基自由基、超氧阴离子3种自由基清除率分别为74.8%、43.2%、89.5%,表明马鞭草总黄酮具有较强的体外抗氧化活性。
Response surface method was used to optimize the extraction technology of total flavonoids from Verbena officinalis L..On the basis of single-factor test,the extraction conditions(ethanol concentration,ratio of water to materials,extracting time and extraction time) were analyzed and optimized by the Box-Behnken design using the comtent of total flavonoids as index.Meanwhile,the antioxidant capacity was measured by scavenging of DPPH·,hydroxyl free radical and superoxide free radical.The results showed that the optimum extraction conditions were as follows: ethanol concentration was 50%,ratio of raw material to liquid was 1∶ 35(g/m L),extracting 2 times,and each for 1.5 min.Under the conditions,the content of total flavonoids was(8.282 ± 0.003) mg/g,which close to predicted content of 8.280 mg/g.This method was suitable for the extraction of total flavonoids from Verbena officinalis L with high extraction rate. When the concentration of total flavonoids was 60 μg/m L,the clearance rate on DPPH·,hydroxyl free radical and superoxide free radical was 74.8%,43.2%,89.5%,which showed that the total flavonoids of Verbena officinalis L.had a certain antioxidant activity.
Response surface method was used to optimize the extraction technology of total flavonoids from Verbena officinalis L..On the basis of single-factor test,the extraction conditions(ethanol concentration,ratio of water to materials,extracting time and extraction time) were analyzed and optimized by the Box-Behnken design using the comtent of total flavonoids as index.Meanwhile,the antioxidant capacity was measured by scavenging of DPPH·,hydroxyl free radical and superoxide free radical.The results showed that the optimum extraction conditions were as follows: ethanol concentration was 50%,ratio of raw material to liquid was 1∶ 35(g/m L),extracting 2 times,and each for 1.5 min.Under the conditions,the content of total flavonoids was(8.282 ± 0.003) mg/g,which close to predicted content of 8.280 mg/g.This method was suitable for the extraction of total flavonoids from Verbena officinalis L with high extraction rate. When the concentration of total flavonoids was 60 μg/m L,the clearance rate on DPPH·,hydroxyl free radical and superoxide free radical was 74.8%,43.2%,89.5%,which showed that the total flavonoids of Verbena officinalis L.had a certain antioxidant activity.
引文
[1]Fan JG.Epidemiology of alcoholic and nonalcoholic fatty liver disease in China[J].J Gastroenterol Hepatol,2013,28(Suppl1):11-17.
[2]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015.
[3]郭琳,苗明三.马鞭草化学、药理及临床应用探讨[J].中医学报,2014,29(9):1345-1347.
[4]陈改敏.HPLC测定不同地区马鞭草中毛蕊花苷的含量[J].光明中医,2015,30(11):2498-2499.
[5]李萌萌,焦天慧,吕长鑫,等.红树莓籽中黄酮提取工艺及抗氧化活性研究[J].中国食品学报,2017,17(2):101-109.
[6]辜中春,李君章,李光荣,等.石楠叶总黄酮含量测定及抗氧化活性研究[J].应用化工,2017,46(12):2488-2491.
[7]王全泽,袁堂丰,刘磊磊,等.响应面法优化闪式提取罗汉松总黄酮及其抗氧化活性[J].精细化工,2018,35(1):65-71.
[8]赛春梅,崔琳琳,杨梦,等.响应曲面法优化超声辅助提取诸葛菜总黄酮工艺及抗氧化活性研究[J].食品与药品,2018,41(1):53-58.
[9]张迪,王勇,王彦兵,等.闪式提取法提取枣果皮中多酚的工艺研究[J].食品工业科技,2013,34(4):259-262.
[10]陈卓君,艾买提·阿曼古力,戴蕴青,等.响应面法优化闪式提取玫瑰黄酮化合物工艺[J].食品工业科技,2011,32(12):387-390.
[11]吴冬梅.闪式提取器在中药研究中的应用[J].中国实验方剂学杂志,2006,12(7):34-37.
[12]张鲁,张媛,王文全,等.甘草地上部分总黄酮提取工艺优化及抗氧化活性研究[J].辽宁中医药大学学报,2018,20(4):55-59.
[13]李会端,江岸,余建中,等.生姜总黄酮甲醇浸提工艺的响应面优化及提取液对羟自由基清除活性[J].北方园艺,2017(23):155-164.
[14]王彦兵,张迪,刘绣华,等.茶多酚-锌(Ⅱ)配合物的合成及抗氧化活性[J].河南大学学报:自然科学版,2012,42(4):378-383.
[15]Chen R Z,Li H P,Li S Z,et al.Extraction optimization,preliminary characterization and immunological activity of polysaccharides from figs[J].International Journal of Biological Macromolecules,2015,72(72):185-194.
[16]Li H,Luo S L,Su J C,et al.Optimization of extraction conditions for flavonoid composition and antioxidant activity of radix scutellariae[J].Analytical Letters,2015(48):1234-1244.
[17]古丽巴哈尔·卡吾力,高晓黎,常占瑛,等.黑果枸杞总黄酮提取工艺优化及抗氧化活性[J].江苏农业科学,2017,45(22):213-217.
[18]寇亮,李璐,陆丽娜,等.响应面法优化柠条锦鸡儿总黄酮超声提取工艺及其体外抗氧化性研究[J].食品工业科技,2016,37(17):225-231.
[19]孟志芬,祝勇,李聪,等.毛泡桐花中总黄酮含量的测定[J].光谱实验室,2012,29(1):221-225.
[20]林三清,周中流,杨红艳,等.柠檬桉叶总黄酮提取工艺及其清除自由基活性研究[J].中国药业,2017,26(6):5-8.
[21]刘薇,王宏君,赵建,等.邻二氮菲-Fe2+法测定保健食品的抗氧化能力[J].食品科学,2010,31(18):333-337.
[22]郭雪峰,岳永德,汤锋,等.用清除超氧阴离子自由基法评价竹叶提取物抗氧化能力[J].光谱学与光谱分析,2008,28(8):1823-1826.
[23]闫蕊,赵桦.黄花油点草总黄酮超声提取工艺的响应面优化及抗氧化性分析[J].食品工业科技,2018,39(10):190-196,203.
[2]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015.
[3]郭琳,苗明三.马鞭草化学、药理及临床应用探讨[J].中医学报,2014,29(9):1345-1347.
[4]陈改敏.HPLC测定不同地区马鞭草中毛蕊花苷的含量[J].光明中医,2015,30(11):2498-2499.
[5]李萌萌,焦天慧,吕长鑫,等.红树莓籽中黄酮提取工艺及抗氧化活性研究[J].中国食品学报,2017,17(2):101-109.
[6]辜中春,李君章,李光荣,等.石楠叶总黄酮含量测定及抗氧化活性研究[J].应用化工,2017,46(12):2488-2491.
[7]王全泽,袁堂丰,刘磊磊,等.响应面法优化闪式提取罗汉松总黄酮及其抗氧化活性[J].精细化工,2018,35(1):65-71.
[8]赛春梅,崔琳琳,杨梦,等.响应曲面法优化超声辅助提取诸葛菜总黄酮工艺及抗氧化活性研究[J].食品与药品,2018,41(1):53-58.
[9]张迪,王勇,王彦兵,等.闪式提取法提取枣果皮中多酚的工艺研究[J].食品工业科技,2013,34(4):259-262.
[10]陈卓君,艾买提·阿曼古力,戴蕴青,等.响应面法优化闪式提取玫瑰黄酮化合物工艺[J].食品工业科技,2011,32(12):387-390.
[11]吴冬梅.闪式提取器在中药研究中的应用[J].中国实验方剂学杂志,2006,12(7):34-37.
[12]张鲁,张媛,王文全,等.甘草地上部分总黄酮提取工艺优化及抗氧化活性研究[J].辽宁中医药大学学报,2018,20(4):55-59.
[13]李会端,江岸,余建中,等.生姜总黄酮甲醇浸提工艺的响应面优化及提取液对羟自由基清除活性[J].北方园艺,2017(23):155-164.
[14]王彦兵,张迪,刘绣华,等.茶多酚-锌(Ⅱ)配合物的合成及抗氧化活性[J].河南大学学报:自然科学版,2012,42(4):378-383.
[15]Chen R Z,Li H P,Li S Z,et al.Extraction optimization,preliminary characterization and immunological activity of polysaccharides from figs[J].International Journal of Biological Macromolecules,2015,72(72):185-194.
[16]Li H,Luo S L,Su J C,et al.Optimization of extraction conditions for flavonoid composition and antioxidant activity of radix scutellariae[J].Analytical Letters,2015(48):1234-1244.
[17]古丽巴哈尔·卡吾力,高晓黎,常占瑛,等.黑果枸杞总黄酮提取工艺优化及抗氧化活性[J].江苏农业科学,2017,45(22):213-217.
[18]寇亮,李璐,陆丽娜,等.响应面法优化柠条锦鸡儿总黄酮超声提取工艺及其体外抗氧化性研究[J].食品工业科技,2016,37(17):225-231.
[19]孟志芬,祝勇,李聪,等.毛泡桐花中总黄酮含量的测定[J].光谱实验室,2012,29(1):221-225.
[20]林三清,周中流,杨红艳,等.柠檬桉叶总黄酮提取工艺及其清除自由基活性研究[J].中国药业,2017,26(6):5-8.
[21]刘薇,王宏君,赵建,等.邻二氮菲-Fe2+法测定保健食品的抗氧化能力[J].食品科学,2010,31(18):333-337.
[22]郭雪峰,岳永德,汤锋,等.用清除超氧阴离子自由基法评价竹叶提取物抗氧化能力[J].光谱学与光谱分析,2008,28(8):1823-1826.
[23]闫蕊,赵桦.黄花油点草总黄酮超声提取工艺的响应面优化及抗氧化性分析[J].食品工业科技,2018,39(10):190-196,203.