辣椒素及二氢辣椒素的制备方法及其结构确证
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摘要
本文建立了一种辣椒素及二氢辣椒素的制备方法。天然辣椒粉经过溶剂提取、结晶纯化得到固态辣椒素类物质,该类物质经中压制备液相单次色谱分离,得到纯度大于96%的辣椒素单体及二氢辣椒素单体。其结构进一步利用核磁、红外等检测手段进行确证。此制备方法操作简单,得到单体的纯度较高,有利于克级及以上规模生产,为辣椒素与二氢辣椒素单体的制备提供了新途径。
A method for the preparation of capsaicin and Dihydrocapsaicin was established. Capsaicinoids were obtained by solvent extraction using chili powder as raw material and were purified by recrystallization. The capsaicin monomer and Dihydrocapsaicin monomer with purity more than 96% were obtained by single-pass liquid chromatography with medium-pressure preparation.Its structure is further confirmed by means of nuclear magnetic resonance and infrared detection. The preparation method is simple and the purity of the monomer is high,which is beneficial to the large-scale production of capsaicin and dihydrocapsaicin,and provides a new way for the preparation of capsaicin and Dihydrocapsaicin monomers.
A method for the preparation of capsaicin and Dihydrocapsaicin was established. Capsaicinoids were obtained by solvent extraction using chili powder as raw material and were purified by recrystallization. The capsaicin monomer and Dihydrocapsaicin monomer with purity more than 96% were obtained by single-pass liquid chromatography with medium-pressure preparation.Its structure is further confirmed by means of nuclear magnetic resonance and infrared detection. The preparation method is simple and the purity of the monomer is high,which is beneficial to the large-scale production of capsaicin and dihydrocapsaicin,and provides a new way for the preparation of capsaicin and Dihydrocapsaicin monomers.
引文
[1]廖妍俨,龙四红,寻思颖,等.液相色谱-荧光检测法测定辣椒制品中的辣椒素和二氢辣椒素[J].中国调味品,2016,41(9):123-125.
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[9]欧阳建勋,刘营营,李忠海,等.辣椒素对储粮害虫防治效果研究[J].食品与机械,2012,28(1):200-204.
[10]钟平生,谢彩玉,张颂声,等. 2种植物素对小鼠的驱避作用研究[J].中华卫生杀虫药械,2011,17(5):332-335.
[11]Srinivasan K. Biological activities of red pepper(Capsicum annuum)and its pungent principle capsaicin:A review[J].Critical Reviews in Food Science and Nutrition,2016,56:1488-1500.
[12]崔桂娟,谢秋涛,李高阳,等.辣椒中辣椒素提取方法与应用[J].食品工业科技,2018,391(3):306-310.
[13]Yao J P,Nair M G,Chandra A. Supercritical carbon dioxide extraction of scotch bonnet(Capsicum annuum)and quantification of capsaicin and Dihydrocapsaicin[J]. Journal of Agricultural and Food Chemistry,1994,42:1303-1305.
[14]董新荣,刘仲华,李本祥,等.制备型高效液相色谱法制备纯化3种辣椒素单体[J].色谱,2008,26(3):366-369.
[15]董新荣,刘仲华,何新益,等.辣椒色素的分离纯化与光学性质的研究[J].食品与机械,2006,22(6):53-55.
[2]李如华,韩振泰.辣椒中辣椒素的高效液相色谱法测定[J].现代科学仪器,2008(6):67-69.
[3]张晶,孙长波,石磊岭,等. RP-HPLC法测定辣椒中辣椒素、二氢辣椒素和降二氢辣椒素含量[J].药物分析杂志,2011,31(2):244-246.
[4]曲鹏豪,王丽娜.国内外催泪弹发展历程及现状[J].化工管理,2014(35):34.
[5]王迪轩.辣椒的药用[J].中国辣椒,2001(2):48.
[6]郑龙辉,陆红佳,刘雄.辣椒素生理功能研究进展[J].食品科学,2011,32(3):262-265.
[7]阳凤,彭燕.辣椒素的临床应用进展[J].临床消化病杂志,2016,28(2):129-132.
[8]姜越君,刘素纯,李佳莲,等.辣椒素的生理功能及提取研究进展[J].安徽农业科学,2009,37(31):15426-15428.
[9]欧阳建勋,刘营营,李忠海,等.辣椒素对储粮害虫防治效果研究[J].食品与机械,2012,28(1):200-204.
[10]钟平生,谢彩玉,张颂声,等. 2种植物素对小鼠的驱避作用研究[J].中华卫生杀虫药械,2011,17(5):332-335.
[11]Srinivasan K. Biological activities of red pepper(Capsicum annuum)and its pungent principle capsaicin:A review[J].Critical Reviews in Food Science and Nutrition,2016,56:1488-1500.
[12]崔桂娟,谢秋涛,李高阳,等.辣椒中辣椒素提取方法与应用[J].食品工业科技,2018,391(3):306-310.
[13]Yao J P,Nair M G,Chandra A. Supercritical carbon dioxide extraction of scotch bonnet(Capsicum annuum)and quantification of capsaicin and Dihydrocapsaicin[J]. Journal of Agricultural and Food Chemistry,1994,42:1303-1305.
[14]董新荣,刘仲华,李本祥,等.制备型高效液相色谱法制备纯化3种辣椒素单体[J].色谱,2008,26(3):366-369.
[15]董新荣,刘仲华,何新益,等.辣椒色素的分离纯化与光学性质的研究[J].食品与机械,2006,22(6):53-55.