翼草景香水煎液对多杀性巴氏杆菌体外抑菌活性及小鼠感染模型的防治效果研究
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摘要
研究翼草景香水煎液对多杀性巴氏杆菌的体外抑菌活性及小鼠感染模型的防治效果。利用传统水煎法制备翼草景香水煎液,用HPLC方法测定翼草景香水煎液中红景天苷浓度,并用红景天苷浓度标定翼草景香水煎液药物浓度,分别采用牛津杯法和试管2倍稀释法测定翼草景香水煎液对多杀性巴氏杆菌抑菌圈直径和最小抑菌浓度(MIC),通过建立死亡率为75%的多杀性巴氏杆菌体内感染小鼠模型,测定翼草景香水煎液浓度为0.12316 mg/m L时,在感染前和感染后给药方式下,分别给与0.1、0.3、0.5 m L药物时对小鼠的体内感染防治作用。结果表明,当翼草景香水煎液浓度为0.49263 mg/m L时,抑菌圈平均直径为28.5 mm; MIC值为0.00385 mg/m L,经病理解剖、细菌镜检、全自动微生物鉴定及药敏系统(VTEK 2 COMPACT)系统细菌鉴定,表明死亡率为75%的多杀性巴氏杆菌体内感染小鼠模型建立成功,当水煎液浓度为0.12316 mg/m L时,0.1、0.3、0.5 m L预防组和治疗组小鼠死亡率分别依次为50%,41.7%,81.8%,66.7%,50%和75%。因此,翼草景香水煎液对多杀性巴氏杆菌具有良好的体内、外抑菌活性,且其预防作用较治疗作用明显。
To clarify the antibacterial activity of the Yicaojingxiang decoction on Pasteurella multocida in vitro and prevention and treatment of infection in vivo. Extracting the effective components with water-extracting in the compound,the content of Salidroside in Yicaojingxiang decoction were measured by HPLC method,and its content indicates the drug concentration of Yicaojingxiang decoction. The antibacterial ring diameters and minimal inhibitory concentration( MIC) were determined by Oxford plate assay system and tube dilution. Establishing the model that lethality rate is 75% of Pasteurella multocida infection in mice and measuring the prevention and treatment of infection in vivo in different doses of the experimental group when the decoction concentration is0.12316 mg/m L. When Yicaojingxiang decoction concentration is 0. 49263 mg/m L,the average diameter of antibacterial rings was 28. 5mm, and the MIC was 0. 00385 mg/m L. Pathological examination, bacterial microscopy,VITEK 2 COMPACT identification after infection showed that the mice infected with Pasteurella multocida model was successfully established. When the concentration of decoction was 0. 12316 mg/m L,the mortality of the 0.1,0.3,0.5 m L prevention group and the treatment group were 50%,41.7%,81.8%,66.7%,50% and 75%,respectively. Comprehensive test results,Yicaojingxiang decoction have a good antibacterial activity on Pasteurella multocida in vitro and in vivo,and its preventive effect is more obvious than the therapeutic effect.
To clarify the antibacterial activity of the Yicaojingxiang decoction on Pasteurella multocida in vitro and prevention and treatment of infection in vivo. Extracting the effective components with water-extracting in the compound,the content of Salidroside in Yicaojingxiang decoction were measured by HPLC method,and its content indicates the drug concentration of Yicaojingxiang decoction. The antibacterial ring diameters and minimal inhibitory concentration( MIC) were determined by Oxford plate assay system and tube dilution. Establishing the model that lethality rate is 75% of Pasteurella multocida infection in mice and measuring the prevention and treatment of infection in vivo in different doses of the experimental group when the decoction concentration is0.12316 mg/m L. When Yicaojingxiang decoction concentration is 0. 49263 mg/m L,the average diameter of antibacterial rings was 28. 5mm, and the MIC was 0. 00385 mg/m L. Pathological examination, bacterial microscopy,VITEK 2 COMPACT identification after infection showed that the mice infected with Pasteurella multocida model was successfully established. When the concentration of decoction was 0. 12316 mg/m L,the mortality of the 0.1,0.3,0.5 m L prevention group and the treatment group were 50%,41.7%,81.8%,66.7%,50% and 75%,respectively. Comprehensive test results,Yicaojingxiang decoction have a good antibacterial activity on Pasteurella multocida in vitro and in vivo,and its preventive effect is more obvious than the therapeutic effect.
引文
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[2]方仁东,雷桂花,杜慧慧,等.多杀性巴氏杆菌毒素的研究进展[J].中国兽医学报,2016,36(11):1990-1996.Fang R D,Lei G H,Du H H,et al.Research progress on the Pasteurella multocida toxin[J].Chinese Veterinary Medicine,2016,36(11):1990-1996.
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[5]高长青,张熙艳,赵攀峰.牛出血性败血症病例报告[J].中国畜牧兽医文摘,2012,28(4):151.Gao C Q,Zhang X Y,Zhao P F.Bovine Hemorrhagic Septicemia case report[J].Chinese Animal Husbandry and Veterinary Abstracts,2012,28(4):151.
[6]斯郎曲措.牛巴氏杆菌病的诊治[J].中国畜牧兽医文摘,2017,33(7):179.S L Q C.Diagnosis and treatment of cattle pasteurellosis[J].Chinese Animal Husbandry and Veterinary Abstracts,2017,33(7):179.
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[9]卫东.芒康县牛出败的治疗与预防对策[J].中国畜牧兽医文摘,2016,32(8):176.Wei D.The treatment and prevention of Bovine Hemorrhagic Septicemia in Markam County[J].Chinese Animal Husbandry and Veterinary Abstracts,2016,32(8):176.
[10]张丽鸿,张红亮,田发益.西藏林芝地区黄牛多杀性巴氏杆菌病的流行病学调查及鉴定[J].甘肃畜牧兽医,2014,44(11):44-46+50.Zhang L H,Zhang H L,Tian F Y.Epidemiological investigation and identification of Pasteurella multocida in cattle in Nyingchi region of Tibet[J].Gansu Animal Husbandry,2014,44(11):44-46+50.
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[15]马玉林.藏药镰形棘豆对缺氧·复氧H9C2心肌细胞损伤的保护作用[D].青海大学,2017.Ma Y L.Protective Effect of Tibetan Medicine Oxytropis falcata Bunge on Hypoxia/Reoxygen(H/R)Injury in H9C2 Cardiomyocytes[D].Qinghai University,2017.
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[21]聂佳,杨文娟,张雯,等.基于数据挖掘技术分析含“三果”藏药配伍规律[J].辽宁中医杂志,2018,45(03):508-511.Nie J,Yang W J,Zhang W,Analysis on Tibetan medicine three fruits compatibility law by data Mining method[J].Liaoning Journal of Traditional Chinese Medicine,2018,45(03):508-511.
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