基于荧光定量PCR法的鸡传染性支气管炎疫苗免疫效果评价研究
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摘要
采用鸡胚传代致弱法得到一株可用于鸡传染性支气管炎疫苗的候选毒株,同时设定一个商品疫苗作为对照,采用荧光定量PCR法评价疫苗的免疫效果。结果表明,攻毒后7 d,两组试验鸡多数脏器均能检测到弱毒,部分脏器检测不到强毒,说明致弱毒株候选疫苗和商品疫苗对试验鸡有很好的保护作用。
A candidate strain of avain infectious bronchitis vaccine was obtained by the method of chicken embryo transfer, and a commercial vaccine was set as the control. The immunofluorescence PCR method was used to evaluate the immune effect of the vaccine. The results showed that after 7 days of the attack, the attenuated virus could be detected in most of viscera organs in the two groups of experimental chickens, but the live virus was hardly detected in some viscera organs, which indicated that the candidate vaccine and the commercial vaccine could prevent the replication of the virulence, so they had better protective effects on the experimental chickens.
A candidate strain of avain infectious bronchitis vaccine was obtained by the method of chicken embryo transfer, and a commercial vaccine was set as the control. The immunofluorescence PCR method was used to evaluate the immune effect of the vaccine. The results showed that after 7 days of the attack, the attenuated virus could be detected in most of viscera organs in the two groups of experimental chickens, but the live virus was hardly detected in some viscera organs, which indicated that the candidate vaccine and the commercial vaccine could prevent the replication of the virulence, so they had better protective effects on the experimental chickens.
引文
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[9] 胡北侠, 杨少华, 许传田, 等. 2008—2012年山东省鸡传染性支气管炎病毒遗传演化分析和致病性研究[J]. 畜牧兽医学报, 2014, 45(5): 795-801.
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[2] Han Z, Sun C, Yan B, et al. A 15-year analysis of molecular epidemiology of avian infectious bronchitis coronavirus in China[J]. Infet. Genet. Evol., 2011, 11(1): 190-200.
[3] Li M, Wang X, Wei P, et al. Serotype and genotype diversity of infectious bronchitis viruses isolated during 1985—2008 in Guangxi, China[J]. Arch. Virol., 2012, 157(3): 467-474.
[4] Mo M L, Li M, Huang B C, et al. Molecular characterization of major structural protein genes of avian coronavirus infectious bronchitis virus isolates in southern China[J]. Viruses, 2013, 5(12): 3007-3020.
[5] Li M, Mo M L, Huang B C, et al. Continuous evolution of avian infectious bronchitis virus resulting in different variants co-circulating in southern China[J]. Arch. Virol., 2013, 158(8): 1783-1786.
[6] 张丽华, 吴翠兰, 张志鹏, 等. 2012—2013年鸡传染性支气管炎病毒广西分离株S1基因序列分析与血清型鉴定[J]. 病毒学报, 2016, 32(1): 62-69.
[7] 秦丽莉, 李孟, 孙蓉, 等. 广西2009—2011年鸡传染性支气管炎病毒分离株的基因分型及血清分型[J]. 病毒学报, 2014, 30(2): 162-170.
[8] 胡北侠, 黄庆华, 杨少华, 等. 鸡传染性支气管炎强毒分离株遗传进化分析和免疫保护试验[J]. 中国动物传染病学报, 2014, 22(3): 7-12.
[9] 胡北侠, 杨少华, 许传田, 等. 2008—2012年山东省鸡传染性支气管炎病毒遗传演化分析和致病性研究[J]. 畜牧兽医学报, 2014, 45(5): 795-801.
[10] 胡北侠. 山东地区鸡传染性支气管炎病毒遗传进化、致病性和抗原变异性分析[D]. 雅安:四川农业大学, 2014. 山东 农业 科学 2019,51(2):124～130 Shandong Agricultural Sciences