鲢鱅鲴混养对水环境及氮素迁移转化的影响
|
摘要
利用~(15)N稳定同位素技术研究混养细鳞斜颌鲴对鲢鱅鱼水环境及氮素迁移转化的影响.结果显示:实验期间,鲴鲢鱅组各营养盐浓度经过短暂升高后迅速降低,实验结束时,TN,TP,NO_3~--N,NO_2~--N,PO_4~(3-)-P浓度分别达到2.67,0.13,0.93,0.026,0.0035mg/L,鲢鱅组各营养盐浓度总体保持增长趋势并于第10d后显著大于鲴鲢鱅组相应值(P<0.05),到实验结束分别为鲴鲢鱅组的1.80,1.69,1.73,1.72,1.83倍.与对照组相比,有鱼组的叶绿素浓度Chla和藻细胞密度明显下降,到实验结束,鲢鱅组的Chl a和藻细胞密度分别为34.69mg/m~3,1.96×10~6cells/L,鲴鲢鱅组分别为25.32mg/m~3,1.9×10~6cells/L,均显著低于对照组(P<0.05).同位素分析结果显示:标记物~(15)N微囊藻进入系统后,部分被鲢鱅鱼摄食同化为机体组成部分,再通过鱼类分泌,排泄等方式进入水体,水体中含氮营养盐被藻类生长吸收,此外,一部分微囊藻沉淀和鱼类排泄物作为沉积腐质被鲴鱼摄食同化为其机体组成部分.
~(15)N stable isotope tracer technique was employed to study the effects of Plagiogathops micrloepis Bleeker(Xenocypris microlepis),Hypophthalmichthys molitrix(Silver carp)and Aristichthys nobilis(bighead carp)polyculture on water environment and nitrogen migration and transformation.The results showed that each nutrient salt concentration in the Xenocypris microlepis,silver carp and bighead carp group decreased rapidly after a brief increase and at the end of the test,while TN,TP,NO_3~--N,NO_2~--N and PO_4~(3-)-P respectively reached 2.67,0.13,0.93,0.026 and 0.0035mg/L.The nutrient concentration of the silver carp and bighead carp group generally maintained an increasing trend and was significantly greater than the corresponding value of the Xenocypris microlepis,silver carp and bighead carp group after the 10th day(P<0.05),while TN,TP,NO_3~--N,NO_2~--N and PO_4~(3-)-P of it were 1.80,1.69,1.73,1.72 and 1.83 times of that group when the experiment was over.Chl a and the cell density of algae in the fish group decreased significantly compared with the control group,while the silver carp and bighead carp group reaching 34.69mg/m~3,1.96×10~6cells/L and the Xenocypris microlepis,silver and bighead carp group reaching 25.32mg/m~3,1.9×10~6 cells/L respectively at the end of the test,which were significantly lower than the control group(P<0.05).The isotope analysis showed that Microcystis marked ~(15)N was assimilated partly by the silver carp and bighead carp body and entered the water body through fish secretion and excretion,then the nitrogen in water were absorbed by algae after being put into the water.In addition,some Microcystis sedimentation and fish excrement were assimilated as the sedimentary humus into the body of Xenocypris microlepis by ingestion.
~(15)N stable isotope tracer technique was employed to study the effects of Plagiogathops micrloepis Bleeker(Xenocypris microlepis),Hypophthalmichthys molitrix(Silver carp)and Aristichthys nobilis(bighead carp)polyculture on water environment and nitrogen migration and transformation.The results showed that each nutrient salt concentration in the Xenocypris microlepis,silver carp and bighead carp group decreased rapidly after a brief increase and at the end of the test,while TN,TP,NO_3~--N,NO_2~--N and PO_4~(3-)-P respectively reached 2.67,0.13,0.93,0.026 and 0.0035mg/L.The nutrient concentration of the silver carp and bighead carp group generally maintained an increasing trend and was significantly greater than the corresponding value of the Xenocypris microlepis,silver carp and bighead carp group after the 10th day(P<0.05),while TN,TP,NO_3~--N,NO_2~--N and PO_4~(3-)-P of it were 1.80,1.69,1.73,1.72 and 1.83 times of that group when the experiment was over.Chl a and the cell density of algae in the fish group decreased significantly compared with the control group,while the silver carp and bighead carp group reaching 34.69mg/m~3,1.96×10~6cells/L and the Xenocypris microlepis,silver and bighead carp group reaching 25.32mg/m~3,1.9×10~6 cells/L respectively at the end of the test,which were significantly lower than the control group(P<0.05).The isotope analysis showed that Microcystis marked ~(15)N was assimilated partly by the silver carp and bighead carp body and entered the water body through fish secretion and excretion,then the nitrogen in water were absorbed by algae after being put into the water.In addition,some Microcystis sedimentation and fish excrement were assimilated as the sedimentary humus into the body of Xenocypris microlepis by ingestion.
引文
[1]Ke Z X,Xie P,Guo L G,et al.In situ study on the control of toxic Microcystis blooms using phytoplanktivorous fish in the subtropical Lake Taihu of China:A large fish pen experiment[J].Aquaculture,2007,265(1):127-138.
[2]李元鹏,于惠莉,顾学林,等.鲢鳙鱼原位修复水库水质的试验[J].净水技术,2017,36(10):52-56.Li Y P,Yu H L,Gu X L,et al.Experiment of reservoir water quality in-situ remediation with silver and bighead carps[J].Water Purification Technology,2017,36(10):52-56.
[3]Starling L D R M.Control of eutrophication by silver carp(Hypophthalmichthys molitrix)in the tropical ParanoáReservoir(Brasília,Brazil):a mesocosm experiment[J].Hydrobiologia,1993,257(3):143-152.
[4]谷孝鸿,刘桂英.滤食性鲢鳙鱼对池塘浮游生物的影响[J].农村生态环境,1996,(1):6-10+41.Gu X H,Liu G Y.Impact of Silver Carp and Head Carp in Fish Ponds on Plankton[J].Rural Ecological Environment,1996,(1):6-10,41.
[5]侯冠军,宋光同,陈静,等.虾蟹混养池塘套养细鳞斜颌鲴试验[J].水产养殖,2012,33(12):18-22.Hou G J,Song G T,Chen J,et al.Text of Shrimp,crab and Plagiogathops micrloepis Bleeker polyculture ponds[J].Journal of Aquaculture,2012,33(12):18-22.
[6]凌俊,江河,胡王,等.池塘蟹、鳜、鲴、鳙健康混养增效模式[J].科学养鱼,2013,(11):25-27.Ling J,Jiang H,Hu W,et al.Pond crab,scorpion,silver carp and bighead carp health polyculture efficiency model[J].Scientific Fish Farming,2013,(11):25-27.
[7]陈军,王煜恒,王会聪,等.投放不同密度的细鳞斜颌鲴对河蟹养殖水质和经济效益的影响[J].江苏农业科学,2017,45(23):174-177.Chen J,Wang Y H,Wang H C,et al.Effects of different density of Plagiogathops micrloepis Bleeker on the water quality and economic benefits of crab farming[J].Jiangsu Agricultural Sciences,2017,45(23):174-177.
[8]杨四秀.鲴鱼的生物学特性及养殖技术[J].内陆水产,2004,(5):28-29.Yang S X.Biological characteristics and culture techniques of Xenocypris microlepis[J].Inland Aquatic Products,2004,(5):28-29.
[9]Burford M A,Preston N P,Glibert P M,et al.Tracing the fate of15N-enriched feed in an intensive shrimp system[J].Aquaculture,2002,206(3):199-216.
[10]Kang C K,Choy E J,Son Y,et al.Food web structure of a restored macroalgal bed in the eastern Korean peninsula determined by C and Nstable isotope analyses[J].Marine Biology,2008,153(6):1181-1198.
[11]王海霞,刘瑀,关春江,等.营养条件对微藻碳,氮稳定同位素组成的影响[J].中国环境科学,2014,34(3):727-733.Wang H X,Liu Y,Guan C J,et al.Effects of nutritional conditions on the stable carbon and nitrogen isotope of microalgae[J].China Environmental Science,2014,34(3):727-733.
[12]王银平,谷孝鸿,曾庆飞,等.食微囊藻干粉鱼类对水环境的影响及氮素迁移转化规律[J].湖泊科学,2015,27(3):475-485.Wang Y P,Gu X H,Zeng Q F,et al.Impacts of faeces from microcystis-dietary silver carp(Hypophthalmichthys molitrix)and tilapia(Oreochromis niloticus)on the aquatic environments and the transferring of faeces-sourced nitrogen[J].J.Lake Sciences,2015,27(3):475-485.
[13]Bergner H,G?tz K P,Simon M,et al.15N-labeling of fishes using 15Nisotopes in aquarium water and the effect of a different protein nutrient on the 15N elimination after the labeling period[J].Archiv für Tierern?hrung,1993,45(2):139.
[14]魏复盛.水和废水监测分析方法(第四版)[M].北京:中国科学出版社,2002:200-284.Wei F S.Water and wastewater monitoring and analysis methods(Fourth edition)[M].Beijing:China Science Press,2002:200-284.
[15]金相灿,屠清瑛.湖泊富营养化调查规范[M].北京:中国环境科学出版社,1990:143.Jin X C,Tu Q Y.Lake eutrophication survey specification[M].Beijing:China Environmental Science Press,1990:143.
[16]陈宇炜,陈开宁,胡耀辉.浮游植物叶绿素a测定的“热乙醇法”及其测定误差的探讨[J].湖泊科学,2006,18(5):550-552.Chen Y W,Chen K N,Hu Y H.Discussion on possible error for phytoplankton chlorophyll-a concentration analysis using hot-ethanol extraction method[J].Journal of Lake Sciences,2006,18(5):550-552.
[17]Dickman M.A new method for making permanent phytoplankton mounts[J].Hydrobiologia,1968,31(2):161-167.
[18]Lehmann M F,Bernasconi S M,Mckenzie J A.A method for the extraction of ammonium from freshwaters for nitrogen isotope analysis[J].Analytical Chemistry,2001,73(19):4717-4721.
[19]吴俊森,王丽丽,贾瑞宝,等.同位素分析法测定硝酸盐氮预处理方法研究[J].山东建筑大学学报,2012,27(1):8-10.Wu J S,Wang L L,Jia R B,et al.Study on pretreatment for measurement nitrate nitrogen in isotopic analysis method[J].Journal of Shandong Jianzhu University,2012,27(1):8-10.
[20]朱雪霞.亿尾鲢鳙鱼苗可“灭”蓝藻120万吨[N].江苏科技报,2015-02-13(A01).Zhu X X.Billions of silver carp and bighead carp fry can"kill"cyanobacteria 1.2 million tons[N].Jiangsu Science and Technology News,2015-02-13(A01).
[21]崔福义,林涛,马放,等.水体治理中鲢鳙生物操纵作用的实验研究[J].南京理工大学学报(自然科学版),2004,(6):668-672.Cui F Y,Lin T,Ma F,et al.Experimental study on the Biological manipulation of silver carp and bighead carp in water treatment[J].Journal of Nanjing University of Science And Technology,2004,(6):668-672.
[22]范振强,崔福义,马华,等.放养鲢鱼预处理高藻原水的除藻效能及特性[J].环境科学,2008,(3):632-637.Fan Z Q,Cui F Y,Ma H,et al.Effectiveness and characteristics of treating algae-laden raw water by stocking silver carp[J].Environmental Science,2008,(3):632-637.
[23]孙晓明,孟庆闻.鲢、鳙滤食及消化器官的发育,构造与食性的相互关系[J].水产学报,1992,(3):202-212.Sun X M,Meng Q W.The relationship between the development of sputum,sputum filter and digestive organs,structure and diet of silver carp and bighead carp[J].Journal of Fisheries of China,1992,(3):202-212.
[24]周洁,林峰.鲢、鳙的食性及其对藻类的消化利用[J].水生生物学报,1990,(2):170-175+197-198.Zhou J,Lin F.The feeding habits of silver carp and bighead carp and their digestion and utilization of algae[J].Acta Hydrobiologica Sinica,1990,(2):170-175+197-198.
[25]陈少莲,刘肖芳,华俐.鲢、鳙在东湖生态系统的氮、磷循环中的作用[J].水生生物学报,1991,(1):8-26.Chen S L,Liu X F,Hua L.The role of silver carp and bighead in the nitrogen and phosphorus cycles of the East Lake ecosystem[J].Acta Hydrobiologica Sinica,1991,(1):8-26.
[26]王嵩,王启山,张丽彬,等.水库大型围隔放养鲢鱼、鳙鱼控藻的研究[J].中国环境科学,2009,29(11):1190-1195.Wang S,Wang Q S,Zhang L B,et al.Large enclosures experimental study on algal control by silver carp and bighead[J].China Environmental Science,2009,29(11):1190-1195.
[27]刘敏,徐敏娴,许迪亮,等.鲢、鳙非经典生物操纵作用的研究进展与应用现状[J].水生态学杂志,2010,31(3):99-103.Liu M,Xu M X,Xu D L,et al.Research progress and application status of non-classical biological manipulation of Hypophthalmichthys molitrix and Aristichthys nobilis[J].Journal of Hydroecology,2010,31(3):99-103.
[28]李艳和,江河,管远亮,等.细鳞斜颌鲴湖泊套养试验[J].安徽农业科学,2005,(11):2085-2086.Li Y H,Jiang H,Guan Y L,et al.Plagiognathops Microlepis Bleeker lake nesting test[J].Anhui Agricultural Sciences,2005,(11):2085-2086.
[29]郭艳敏,高月香,张毅敏,等.鲴对食微囊藻鲢鳙排泄物及藻活性的作用研究[J].中国环境科学,2016,36(12):3784-3792.Guo Y M,Gao Y X,Zhang Y M,et al.Effect of Xenocypris microlepis on feces and microcystis activity from microcystis-dietary silver carp and bighead carp[J].China Environmental Science,2016,36(12):3784-3792.
[30]胡湛波,刘成,周权能,等.曝气对生物促生剂修复城市黑臭河道水体的影响[J].环境工程学报,2012,6(12):4281-4288.Hu Z B,Liu C,Zhou Q N,et al.Effects of aeration to remediation of black-odorous urban river by using biostimulants[J].Chinese Journal of Environmental Engineering,2012,6(12):4281-4288.
[31]吴晗,张毅敏,周创,等.温度对细鳞斜颌鲴生理学特性影响的研究[J].环境工程,2015,33(5):23-27+89.Wu H,Zhang Y M,Zhou C,et al.Influence of temperature on physiological Characteristics of Plagiognathops Microlepis Bleeker[J].Environmental Engineering,2015,33(5):23-27+89.
[32]Gribsholt B,Boschker S,Struyf E,et al.Nitrogen processing in a tidal freshwater marsh:A whole-ecosystem 15N labeling study[J].Limnology and Oceanography,2005,50(6):1945-1959.
[33]郭艳敏.鲢鳙鲴组合系统控制富营养化水体中蓝藻活性和氮素迁移转化的研究[D].南京:东南大学,2017.Guo Y M.Studies on the variation of cyanophyta activity and nitrogen migration and transformation in biomanipulation process by silver carp,bighead carp and Xenocypris microlepis[D].Nanjing:Southeast University,2017.
[34]Burford M A,Glibert P M.Short-term nitrogen uptake and regeneration in early and late growth phase shrimp ponds[J].Aquaculture Research,1999,30(3):215-227.
[35]吴轩浩,高佳逸,严杨蔚,等.无机氮和有机氮对铜绿微囊藻生长和产毒影响的比较[J].环境科学学报,2015,35(3):677-683.Wu X H,Gao J Y,Yan Y W,et al.Comparison of inorganic nitrogen and organic nitrogen on the growth and microcystin production of Microcystis aeruginosa[J].Acta Scientiae Circumstantiae,2015,35(3):677-683.
[36]李柯.蓝藻分解所释放营养盐在沉水植被区的归趋[D].武汉:华中农业大学,2011.Li K.The fate of nutrients released during the decomposing of cyanobacterial detritus in submerged macrophytes zones[D].Wuhan:Huazhong Agricultural University,2011.
[2]李元鹏,于惠莉,顾学林,等.鲢鳙鱼原位修复水库水质的试验[J].净水技术,2017,36(10):52-56.Li Y P,Yu H L,Gu X L,et al.Experiment of reservoir water quality in-situ remediation with silver and bighead carps[J].Water Purification Technology,2017,36(10):52-56.
[3]Starling L D R M.Control of eutrophication by silver carp(Hypophthalmichthys molitrix)in the tropical ParanoáReservoir(Brasília,Brazil):a mesocosm experiment[J].Hydrobiologia,1993,257(3):143-152.
[4]谷孝鸿,刘桂英.滤食性鲢鳙鱼对池塘浮游生物的影响[J].农村生态环境,1996,(1):6-10+41.Gu X H,Liu G Y.Impact of Silver Carp and Head Carp in Fish Ponds on Plankton[J].Rural Ecological Environment,1996,(1):6-10,41.
[5]侯冠军,宋光同,陈静,等.虾蟹混养池塘套养细鳞斜颌鲴试验[J].水产养殖,2012,33(12):18-22.Hou G J,Song G T,Chen J,et al.Text of Shrimp,crab and Plagiogathops micrloepis Bleeker polyculture ponds[J].Journal of Aquaculture,2012,33(12):18-22.
[6]凌俊,江河,胡王,等.池塘蟹、鳜、鲴、鳙健康混养增效模式[J].科学养鱼,2013,(11):25-27.Ling J,Jiang H,Hu W,et al.Pond crab,scorpion,silver carp and bighead carp health polyculture efficiency model[J].Scientific Fish Farming,2013,(11):25-27.
[7]陈军,王煜恒,王会聪,等.投放不同密度的细鳞斜颌鲴对河蟹养殖水质和经济效益的影响[J].江苏农业科学,2017,45(23):174-177.Chen J,Wang Y H,Wang H C,et al.Effects of different density of Plagiogathops micrloepis Bleeker on the water quality and economic benefits of crab farming[J].Jiangsu Agricultural Sciences,2017,45(23):174-177.
[8]杨四秀.鲴鱼的生物学特性及养殖技术[J].内陆水产,2004,(5):28-29.Yang S X.Biological characteristics and culture techniques of Xenocypris microlepis[J].Inland Aquatic Products,2004,(5):28-29.
[9]Burford M A,Preston N P,Glibert P M,et al.Tracing the fate of15N-enriched feed in an intensive shrimp system[J].Aquaculture,2002,206(3):199-216.
[10]Kang C K,Choy E J,Son Y,et al.Food web structure of a restored macroalgal bed in the eastern Korean peninsula determined by C and Nstable isotope analyses[J].Marine Biology,2008,153(6):1181-1198.
[11]王海霞,刘瑀,关春江,等.营养条件对微藻碳,氮稳定同位素组成的影响[J].中国环境科学,2014,34(3):727-733.Wang H X,Liu Y,Guan C J,et al.Effects of nutritional conditions on the stable carbon and nitrogen isotope of microalgae[J].China Environmental Science,2014,34(3):727-733.
[12]王银平,谷孝鸿,曾庆飞,等.食微囊藻干粉鱼类对水环境的影响及氮素迁移转化规律[J].湖泊科学,2015,27(3):475-485.Wang Y P,Gu X H,Zeng Q F,et al.Impacts of faeces from microcystis-dietary silver carp(Hypophthalmichthys molitrix)and tilapia(Oreochromis niloticus)on the aquatic environments and the transferring of faeces-sourced nitrogen[J].J.Lake Sciences,2015,27(3):475-485.
[13]Bergner H,G?tz K P,Simon M,et al.15N-labeling of fishes using 15Nisotopes in aquarium water and the effect of a different protein nutrient on the 15N elimination after the labeling period[J].Archiv für Tierern?hrung,1993,45(2):139.
[14]魏复盛.水和废水监测分析方法(第四版)[M].北京:中国科学出版社,2002:200-284.Wei F S.Water and wastewater monitoring and analysis methods(Fourth edition)[M].Beijing:China Science Press,2002:200-284.
[15]金相灿,屠清瑛.湖泊富营养化调查规范[M].北京:中国环境科学出版社,1990:143.Jin X C,Tu Q Y.Lake eutrophication survey specification[M].Beijing:China Environmental Science Press,1990:143.
[16]陈宇炜,陈开宁,胡耀辉.浮游植物叶绿素a测定的“热乙醇法”及其测定误差的探讨[J].湖泊科学,2006,18(5):550-552.Chen Y W,Chen K N,Hu Y H.Discussion on possible error for phytoplankton chlorophyll-a concentration analysis using hot-ethanol extraction method[J].Journal of Lake Sciences,2006,18(5):550-552.
[17]Dickman M.A new method for making permanent phytoplankton mounts[J].Hydrobiologia,1968,31(2):161-167.
[18]Lehmann M F,Bernasconi S M,Mckenzie J A.A method for the extraction of ammonium from freshwaters for nitrogen isotope analysis[J].Analytical Chemistry,2001,73(19):4717-4721.
[19]吴俊森,王丽丽,贾瑞宝,等.同位素分析法测定硝酸盐氮预处理方法研究[J].山东建筑大学学报,2012,27(1):8-10.Wu J S,Wang L L,Jia R B,et al.Study on pretreatment for measurement nitrate nitrogen in isotopic analysis method[J].Journal of Shandong Jianzhu University,2012,27(1):8-10.
[20]朱雪霞.亿尾鲢鳙鱼苗可“灭”蓝藻120万吨[N].江苏科技报,2015-02-13(A01).Zhu X X.Billions of silver carp and bighead carp fry can"kill"cyanobacteria 1.2 million tons[N].Jiangsu Science and Technology News,2015-02-13(A01).
[21]崔福义,林涛,马放,等.水体治理中鲢鳙生物操纵作用的实验研究[J].南京理工大学学报(自然科学版),2004,(6):668-672.Cui F Y,Lin T,Ma F,et al.Experimental study on the Biological manipulation of silver carp and bighead carp in water treatment[J].Journal of Nanjing University of Science And Technology,2004,(6):668-672.
[22]范振强,崔福义,马华,等.放养鲢鱼预处理高藻原水的除藻效能及特性[J].环境科学,2008,(3):632-637.Fan Z Q,Cui F Y,Ma H,et al.Effectiveness and characteristics of treating algae-laden raw water by stocking silver carp[J].Environmental Science,2008,(3):632-637.
[23]孙晓明,孟庆闻.鲢、鳙滤食及消化器官的发育,构造与食性的相互关系[J].水产学报,1992,(3):202-212.Sun X M,Meng Q W.The relationship between the development of sputum,sputum filter and digestive organs,structure and diet of silver carp and bighead carp[J].Journal of Fisheries of China,1992,(3):202-212.
[24]周洁,林峰.鲢、鳙的食性及其对藻类的消化利用[J].水生生物学报,1990,(2):170-175+197-198.Zhou J,Lin F.The feeding habits of silver carp and bighead carp and their digestion and utilization of algae[J].Acta Hydrobiologica Sinica,1990,(2):170-175+197-198.
[25]陈少莲,刘肖芳,华俐.鲢、鳙在东湖生态系统的氮、磷循环中的作用[J].水生生物学报,1991,(1):8-26.Chen S L,Liu X F,Hua L.The role of silver carp and bighead in the nitrogen and phosphorus cycles of the East Lake ecosystem[J].Acta Hydrobiologica Sinica,1991,(1):8-26.
[26]王嵩,王启山,张丽彬,等.水库大型围隔放养鲢鱼、鳙鱼控藻的研究[J].中国环境科学,2009,29(11):1190-1195.Wang S,Wang Q S,Zhang L B,et al.Large enclosures experimental study on algal control by silver carp and bighead[J].China Environmental Science,2009,29(11):1190-1195.
[27]刘敏,徐敏娴,许迪亮,等.鲢、鳙非经典生物操纵作用的研究进展与应用现状[J].水生态学杂志,2010,31(3):99-103.Liu M,Xu M X,Xu D L,et al.Research progress and application status of non-classical biological manipulation of Hypophthalmichthys molitrix and Aristichthys nobilis[J].Journal of Hydroecology,2010,31(3):99-103.
[28]李艳和,江河,管远亮,等.细鳞斜颌鲴湖泊套养试验[J].安徽农业科学,2005,(11):2085-2086.Li Y H,Jiang H,Guan Y L,et al.Plagiognathops Microlepis Bleeker lake nesting test[J].Anhui Agricultural Sciences,2005,(11):2085-2086.
[29]郭艳敏,高月香,张毅敏,等.鲴对食微囊藻鲢鳙排泄物及藻活性的作用研究[J].中国环境科学,2016,36(12):3784-3792.Guo Y M,Gao Y X,Zhang Y M,et al.Effect of Xenocypris microlepis on feces and microcystis activity from microcystis-dietary silver carp and bighead carp[J].China Environmental Science,2016,36(12):3784-3792.
[30]胡湛波,刘成,周权能,等.曝气对生物促生剂修复城市黑臭河道水体的影响[J].环境工程学报,2012,6(12):4281-4288.Hu Z B,Liu C,Zhou Q N,et al.Effects of aeration to remediation of black-odorous urban river by using biostimulants[J].Chinese Journal of Environmental Engineering,2012,6(12):4281-4288.
[31]吴晗,张毅敏,周创,等.温度对细鳞斜颌鲴生理学特性影响的研究[J].环境工程,2015,33(5):23-27+89.Wu H,Zhang Y M,Zhou C,et al.Influence of temperature on physiological Characteristics of Plagiognathops Microlepis Bleeker[J].Environmental Engineering,2015,33(5):23-27+89.
[32]Gribsholt B,Boschker S,Struyf E,et al.Nitrogen processing in a tidal freshwater marsh:A whole-ecosystem 15N labeling study[J].Limnology and Oceanography,2005,50(6):1945-1959.
[33]郭艳敏.鲢鳙鲴组合系统控制富营养化水体中蓝藻活性和氮素迁移转化的研究[D].南京:东南大学,2017.Guo Y M.Studies on the variation of cyanophyta activity and nitrogen migration and transformation in biomanipulation process by silver carp,bighead carp and Xenocypris microlepis[D].Nanjing:Southeast University,2017.
[34]Burford M A,Glibert P M.Short-term nitrogen uptake and regeneration in early and late growth phase shrimp ponds[J].Aquaculture Research,1999,30(3):215-227.
[35]吴轩浩,高佳逸,严杨蔚,等.无机氮和有机氮对铜绿微囊藻生长和产毒影响的比较[J].环境科学学报,2015,35(3):677-683.Wu X H,Gao J Y,Yan Y W,et al.Comparison of inorganic nitrogen and organic nitrogen on the growth and microcystin production of Microcystis aeruginosa[J].Acta Scientiae Circumstantiae,2015,35(3):677-683.
[36]李柯.蓝藻分解所释放营养盐在沉水植被区的归趋[D].武汉:华中农业大学,2011.Li K.The fate of nutrients released during the decomposing of cyanobacterial detritus in submerged macrophytes zones[D].Wuhan:Huazhong Agricultural University,2011.