喉痹中药组方规律探索及整合药理学分子机制
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摘要
目的采用中医传承辅助系统对喉痹新方进行数据挖掘,通过整合药理学平台对方剂的潜在分子靶点和信号通路进行分析。方法运用数据库的标准化方式汇编中草药处方,采用度量关联规则强度的方法及联合复杂系统方法学提供的熵聚类方法,计算分析得到相关药物的出现频率及关联规则,从而计算得到新方。在整合药理学平台目标预测和网络建设的功能帮助下,得到新方的成分-靶标网络、组方-喉痹相关靶标网络,并对新方作用的喉痹相关靶标分子机制进行分析。结果共收集了177个处方,确定处方中药使用频率和药物间的关联规律。获得5个新的处方,并对重点处方比较了可能的分子机制。结论结合中医传承辅助系统和整合药理学平台,可以从宏观和微观的角度挖掘新方剂,研究其潜在分子机制,寻找间接存在的影响因素,为研究提供方向指南。
Objective To carry out data mining on the new prescriptions of pharyngitis, use traditional Chinese medicine inheritance assistant system and analyze the potential molecular targets and signaling pathways of the prescription by using integrated pharmacology platform. Methods The prescriptions of Chinese herbal medicines were collected through database integration.The association rules and the complex system entropy clustering method were used to determine the frequency and association rules of each drug and to find out the new prescriptions.By integrating the functions of the pharmacology platform and network construction, we obtained the target network and analyzed the molecular mechanism. Results A total of 177 prescriptions were collected to analyze the association between the using frequency of TCM prescriptions and the herbs.Five new prescriptions were obtained and the possible molecular mechanisms were compared. Conclusion Combining the traditional Chinese medicine inheritance assistant system and the integrated pharmacology platform, the new prescriptions can be explored from macrocosmic and microscopic perspectives for potential molecular mechanisms, to provide guidance for further research.
Objective To carry out data mining on the new prescriptions of pharyngitis, use traditional Chinese medicine inheritance assistant system and analyze the potential molecular targets and signaling pathways of the prescription by using integrated pharmacology platform. Methods The prescriptions of Chinese herbal medicines were collected through database integration.The association rules and the complex system entropy clustering method were used to determine the frequency and association rules of each drug and to find out the new prescriptions.By integrating the functions of the pharmacology platform and network construction, we obtained the target network and analyzed the molecular mechanism. Results A total of 177 prescriptions were collected to analyze the association between the using frequency of TCM prescriptions and the herbs.Five new prescriptions were obtained and the possible molecular mechanisms were compared. Conclusion Combining the traditional Chinese medicine inheritance assistant system and the integrated pharmacology platform, the new prescriptions can be explored from macrocosmic and microscopic perspectives for potential molecular mechanisms, to provide guidance for further research.
引文
[1] 国家中医药管理局.中华人民共和国中医药行业标准中医药诊断疗效标准[M].南京:南京大学出版社,1994:126-127.
[2] 卢朋,李健,唐仕欢.中医传承辅助系统软件开发与应用[J].中国实验方剂学杂志,2012,18(9):1-4.
[3] 杨洪军,许海玉.整合药理学-元胡止痛方的探索[M].北京:科学出版社,2015:11-12.
[4] 许海玉,杨洪军.整合药理学:中药现代研究新模式[J].中国中药杂志,2014,39(3):357-362.
[5] 陈屏,徐东铭,雷军.麦冬化学成分及药理作用的研究现状[J].长春中医学院学报,2004,20(1):35-36.
[6] 冯卫生,李孟,郑晓珂,等.生地黄化学成分研究[J].中国药学杂志,2014,49(17):1496-1502.
[7] 童志远,颜晓燕.贝母化学成分及质量控制方法研究进展[J].西南军医,2009,11(2):260-261.
[8] 曹春泉.牡丹皮的化学成分研究进展[J].广州化工,2013,41(12):44-45,51.
[9] 孟祥乐,李红伟,李颜,等.栀子化学成分及其药理作用研究进展[J].中国新药杂志,2011,20(11):959-967.
[10] 郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[11] 周松,刘永刚,张国祥,等.黄柏化学成分及质量控制研究进展[J].中国药房,2012,23(39):3740-3742.
[12] 傅兴圣,陈菲,刘训红,等.大黄化学成分与药理作用研究新进展[J].中国新药杂志,2011,20(16):1534-1538,1568.
[13] 胡玉顺.198例慢性咽炎患者咽分泌物调查分析[J].中华医院感染学杂志,2010,20(24):3934-3935.
[14] 杨霄.中西医治疗急慢性咽喉炎现状[J].河南中医,2014,34(1):172-175.
[15] 邓燕飞.慢性咽喉炎与胃肠道疾病[J].中国眼耳鼻喉科杂志,1999,4(3):135-136.
[16] 张莉.上呼吸道微生态平衡研究与益生菌制剂[J].内蒙古大学学报(自然科学版),2006,37(6):714-718.
[17] 余鸿亮.微生态制剂整肠生治疗耳鼻咽喉疾病200例临床疗效观察[J].中国微生态学杂志,1998,10(6):359-361.
[18] 欧阳万金,王蕾,肖航,等.谷氨酰胺对动物肠道微生态影响的研究进展[J].江西饲料,2013,(6):1-3.
[19] 肖硕,王宗喜.抗菌药物在急性咽炎中的合理应用[J].医药导报,2013,32(5):641-643.
[2] 卢朋,李健,唐仕欢.中医传承辅助系统软件开发与应用[J].中国实验方剂学杂志,2012,18(9):1-4.
[3] 杨洪军,许海玉.整合药理学-元胡止痛方的探索[M].北京:科学出版社,2015:11-12.
[4] 许海玉,杨洪军.整合药理学:中药现代研究新模式[J].中国中药杂志,2014,39(3):357-362.
[5] 陈屏,徐东铭,雷军.麦冬化学成分及药理作用的研究现状[J].长春中医学院学报,2004,20(1):35-36.
[6] 冯卫生,李孟,郑晓珂,等.生地黄化学成分研究[J].中国药学杂志,2014,49(17):1496-1502.
[7] 童志远,颜晓燕.贝母化学成分及质量控制方法研究进展[J].西南军医,2009,11(2):260-261.
[8] 曹春泉.牡丹皮的化学成分研究进展[J].广州化工,2013,41(12):44-45,51.
[9] 孟祥乐,李红伟,李颜,等.栀子化学成分及其药理作用研究进展[J].中国新药杂志,2011,20(11):959-967.
[10] 郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[11] 周松,刘永刚,张国祥,等.黄柏化学成分及质量控制研究进展[J].中国药房,2012,23(39):3740-3742.
[12] 傅兴圣,陈菲,刘训红,等.大黄化学成分与药理作用研究新进展[J].中国新药杂志,2011,20(16):1534-1538,1568.
[13] 胡玉顺.198例慢性咽炎患者咽分泌物调查分析[J].中华医院感染学杂志,2010,20(24):3934-3935.
[14] 杨霄.中西医治疗急慢性咽喉炎现状[J].河南中医,2014,34(1):172-175.
[15] 邓燕飞.慢性咽喉炎与胃肠道疾病[J].中国眼耳鼻喉科杂志,1999,4(3):135-136.
[16] 张莉.上呼吸道微生态平衡研究与益生菌制剂[J].内蒙古大学学报(自然科学版),2006,37(6):714-718.
[17] 余鸿亮.微生态制剂整肠生治疗耳鼻咽喉疾病200例临床疗效观察[J].中国微生态学杂志,1998,10(6):359-361.
[18] 欧阳万金,王蕾,肖航,等.谷氨酰胺对动物肠道微生态影响的研究进展[J].江西饲料,2013,(6):1-3.
[19] 肖硕,王宗喜.抗菌药物在急性咽炎中的合理应用[J].医药导报,2013,32(5):641-643.