国产血竭、进口血竭中微量元素的分布规律及相关性分析
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摘要
目的:测定国产血竭、进口血竭药材中10种微量元素含量,研究各微量元素的分布规律和相关性。方法:微波消解法处理样品,利用火焰原子吸收法和石墨炉原子吸收法测定样品中Fe、Cu、Zn、Mg、Ca、Sr、K、Pb、Cd、Cr共10种微量元素含量,通过Radar图分析法和Spearman等级相关系数解析2种血竭中各元素分布规律和相关性。结果:元素加标回收率在96.7%～104.9%范围,RSD<2%。Radar图分析发现,Fe、K、Ca在2种血竭中分布最为广泛(0～4 000μg·g~(-1)),Cu、Mg、Sr、Zn次之(0～140μg·g~(-1)),进口血竭中K、Ca、Fe、Mg、Cu含量高于国产血竭,而Sr、Zn含量较低。国产血竭中有较高含量的重金属元素Cr,重金属元素Pb、Cd在2种血竭中分布相当(0～0.10μg·g~(-1));Spearman相关性分析表明,国产血竭中Cu-Fe、CuZn、Fe-Zn相关系数高达0.9以上水平(P<0.01),而进口血竭中,Cr-Fe、Cr-Mg、Cr-Zn则呈现极显著负相关(P<0.01)。结论:本研究建立的元素测定方法准确、可靠,2种血竭中元素分布具有规律性,药效相关元素间呈现不同程度的相关性,可以为2种血竭的临床使用和药效分析研究提供参考。
Objective:To determine and investigate the distribution and correlation of 10 trace elements in Draconis Resina and Draconis Sanguis. Methods:Samples were processed by microwave digestion. The content of 10 trace elements,Cu,Zn,Fe Mg,Ca,Sr,K,Pb,Cd and Cr,were determined by flame atomic absorption spectrometry and graphite furnace atomic absorption spectrometry. The distribution and correlation of elements in two kinds of "dragon's blood" were analyzed by Radar chart and Spearman rank correlation coefficient. Results:Element recoveries were in the range of 96.7%-104.9% with RSDs<2%. Radar analysis showed that Fe,K and Ca were most widely distributed in two kinds of "dragon's blood"(0-4 000 μg·g~(-1)),followed by Cu,Mg,Sr and Zn(0-140 μg·g~(-1)). The contents of K,Ca,Fe,Mg and Cu in the Draconis Sanguis were higher than those in Draconis Resina,while the contents of Sr and Zn were lower. There was a higher content of heavy metal elements Cr in Resina Draconis,and heavy metal element Cd,Pb were distributed equivalently in two"dragon's blood"(0-0.10 μg·g~(-1));Spearman correlation analysis showed that the correlation coefficients of Cu-Fe,Cu-Zn and Fe-Zn in Resina Draconis were more than 0.9(P<0.01),while Cr-Fe、Cr-Mg and Cr-Zn in Draconis Sanguis showed significant negative correlations(P<0.01). Conclusion:The method of element determination established in this study is accurate and reliable. The distribution of elements in two kinds of"dragon's blood" is regular,and the elements correlating to drug efficacy have different degrees of correlation,which provides an important basis for the clinical use and pharmacodynamic analysis of two kinds of "dragon's blood".
Objective:To determine and investigate the distribution and correlation of 10 trace elements in Draconis Resina and Draconis Sanguis. Methods:Samples were processed by microwave digestion. The content of 10 trace elements,Cu,Zn,Fe Mg,Ca,Sr,K,Pb,Cd and Cr,were determined by flame atomic absorption spectrometry and graphite furnace atomic absorption spectrometry. The distribution and correlation of elements in two kinds of "dragon's blood" were analyzed by Radar chart and Spearman rank correlation coefficient. Results:Element recoveries were in the range of 96.7%-104.9% with RSDs<2%. Radar analysis showed that Fe,K and Ca were most widely distributed in two kinds of "dragon's blood"(0-4 000 μg·g~(-1)),followed by Cu,Mg,Sr and Zn(0-140 μg·g~(-1)). The contents of K,Ca,Fe,Mg and Cu in the Draconis Sanguis were higher than those in Draconis Resina,while the contents of Sr and Zn were lower. There was a higher content of heavy metal elements Cr in Resina Draconis,and heavy metal element Cd,Pb were distributed equivalently in two"dragon's blood"(0-0.10 μg·g~(-1));Spearman correlation analysis showed that the correlation coefficients of Cu-Fe,Cu-Zn and Fe-Zn in Resina Draconis were more than 0.9(P<0.01),while Cr-Fe、Cr-Mg and Cr-Zn in Draconis Sanguis showed significant negative correlations(P<0.01). Conclusion:The method of element determination established in this study is accurate and reliable. The distribution of elements in two kinds of"dragon's blood" is regular,and the elements correlating to drug efficacy have different degrees of correlation,which provides an important basis for the clinical use and pharmacodynamic analysis of two kinds of "dragon's blood".
引文
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[2]陆晖,滕建北,吴怀恩.国产血竭研究概况[J].中药材,2003,12(6):459LU H,TENG JB,WU HE.A review on the study of Resina Draconis produced in China[J].J Chin Med Mater,2003,12(6):459
[3]YI T,CHEN HB,ZHAO ZZ,et al.Comparison of the chemical profiles and anti-platelet aggregation effects of two“Dragon’s Blood”drugs used in traditional Chinese medicine[J].J Ethnopharmacol,2011,133(2):796
[4]韩丽琴.中药总黄酮与微量元素聚类分析及其药效机理的研究[D].延吉:延边大学,2011HAN LQ.Study on the Cluster Analysis of Total Flavonoids and Trace Elements in Chinese Medicine and the Mechanism of Its Efficacy[D].Yanji:Yanbian University,2011
[5]王懿萍,张小荣,王盛民,等.中药微量元素与药效的关系[J].陕西中医,2006,27(12):1573WANG YP,ZHANG XR,WANG SM,et al.The relationship between trace elements and efficacy of traditional Chinese Medicine[J].Shaanxi Tradit Chin Med,2006,27(12):1573
[6]张柯瑶,郭惠,王媚,等.扶正固本类中药中微量元素含量测定及药效关系研究[J].陕西中医,2017,38(3):398ZHANG KY,GUO H,WANG M,et al.Determination of trace elements in Chinese herbs for strengthening the body resistance and study on its effect relationship[J].Shaanxi Tradit Chin Med,2017,38(3):398
[7]钟进芳.六味活血祛瘀类中药微量元素水平及形态的研究[D].兰州:西北民族大学,2010ZHONG JF.Study on the Level and Morphology of Trace Elements of Six Chinese Herbs for Activating Blood and Removing Stasis[D].Lanzhou:Northwest University For Nationalities,2010
[8]李德永,张勇.活血中药微量元素的比较研究[J].光谱实验室,2010,27(2):558LI DY,ZHANG Y.Comparative study of trace elements in Chinese herbs for activating blood and removing stasis[J].Chin J Spectrosc Lab,2010,27(2):558
[9]尹鲁生,戴乐岁.十五种活血化瘀中草药中微量元素的测定[J].中成药研究,1988(4):28YIN LS,DAI LS.Determination of trace elements in fifteen kinds of Chinese herbal medicine for activating blood and removing stasis[J].Chin Tradit Pat Med Res,1988(4):28
[10]张秀娟,芦清,何春阳.药材中微量元素测定方法的研究进展[J].药物评价研究,2017,40(4):566ZHANG XJ,LU Q,HE CY.Progress in the determination of trace elements in medicinal materials[J].Study Drug Eval,2017,40(4):566
[11]杨梅,冯芳.中药中微量元素分析方法及其应用[J].药学进展,2008,32(2):56YANG M,FENG F.Analysis of trace elements in traditional Chinese medicine and its application[J].Prog Pharm,2008,32(2):56
[12]刘雯,李峰,张天锡,等.中药微量元素检测常用方法及其数据统计分析和应用[J].山东中医杂志,2016,35(12):1062LIU W,LI F,ZHANG TX,et al.The common methods for the detection of trace elements in Chinese medicine and the statistical analysis and application of the data[J].J Shandong Tradit Chin Med,2016,35(12):1062
[13]中华人民共和国药典2015年版.四部[S].2015:41ChP 2015.VolⅣ[S].2015:41
[14]CHAUMILLON R,ROMEAS T,PAILLARD C,et al.Enhancing data visualisation to capture the simulator sickness phenomenon:on the usefulness of radar charts[J].Data Brief,2017,13:301
[15]WARRENS MJ.Transforming intraclass correlation coefficients with the spearman-brown formula[J].J Clin Epidemiol,2017,85(3):14