3株白纹伊蚊生物学特性及对寨卡病毒易感性的比较
|
摘要
目的探究3个地理株白纹伊蚊的生物学特性及其对寨卡病毒(ZIKV)的易感性差异。方法建立广州株(GZ株)、深圳株(SZ株)和恩平株(EP株)白纹伊蚊,观察记录3株蚊株的生长发育周期;检测3个蚊株Wolbachia携带率和其组织分布;经口感染ZIKV后,检测不同地理株白纹伊蚊对ZIKV的易感性。结果 3个地理株白纹伊蚊生长发育周期无显著差异且均携带Wolbachia,但SZ株和GZ株白纹伊蚊组织内Wolbachia含量高于EP株,差异有统计学意义(P<0.05)。经口感染ZIKV后,3个地理株的白纹伊蚊均可有效传播ZIKV,GZ株白纹伊蚊对ZIKV的感染率最高为91.7%,传播率为66.7%;EP株白纹伊蚊对ZIKV的传播能力最弱,传播率为54.5%。结论不同地理株白纹伊蚊对ZIKV的易感性不同,可能与其组织内Wolbachia含量有关。
Objective To determine the biological characteristics of three geographically Aedes albopictus strains and their susceptibility to Zika virus(ZIKV). Methods Three strains of Aedes albopictus,Guangzhou(GZ),Shenzhen(SZ)and Enping(EP)were established in the laboratory. The infection rate and somatic tissue distribution of Wolbachia in three mosquito strains were detected. The oral susceptibility of local Aedes albopictus to ZIKV was measured. Results There was no significant difference in the growth cycle among three geographic Aedes albopictus strains. All of them were naturally super infected with Wolbachia,w AlbA and w AlbB,but Wolbachia density in the somatic tissue of Aedes albopictus SZ strain and GZ strain were significantly higher than that of EP strain(P<0.05). After oral infection to Asian ZIKV,three geographic strains of Aedes albopictus could effectively transmit ZIKV. GZ strain was the most susceptible to ZIKV with infection rate91.7% and transmission rate 66.7%. However,the EP strain showed weak transmission ability(54.5%) to ZIKV.Conclusion The susceptibility of three Aedes albopictus strains to ZIKV is different and may be correlated to the Wolbachia density in mosquito tissues.
Objective To determine the biological characteristics of three geographically Aedes albopictus strains and their susceptibility to Zika virus(ZIKV). Methods Three strains of Aedes albopictus,Guangzhou(GZ),Shenzhen(SZ)and Enping(EP)were established in the laboratory. The infection rate and somatic tissue distribution of Wolbachia in three mosquito strains were detected. The oral susceptibility of local Aedes albopictus to ZIKV was measured. Results There was no significant difference in the growth cycle among three geographic Aedes albopictus strains. All of them were naturally super infected with Wolbachia,w AlbA and w AlbB,but Wolbachia density in the somatic tissue of Aedes albopictus SZ strain and GZ strain were significantly higher than that of EP strain(P<0.05). After oral infection to Asian ZIKV,three geographic strains of Aedes albopictus could effectively transmit ZIKV. GZ strain was the most susceptible to ZIKV with infection rate91.7% and transmission rate 66.7%. However,the EP strain showed weak transmission ability(54.5%) to ZIKV.Conclusion The susceptibility of three Aedes albopictus strains to ZIKV is different and may be correlated to the Wolbachia density in mosquito tissues.
引文
[1]Dick GW,Kitchen SF,Haddow AJ.Zika virus.I.Isolations and serological specificity[J].Trans R Soc Trop Med Hyg,1952,46(5):509-520.
[2]Smithburn KC.Neutralizing antibodies against certain recently isolated viruses in the sera of human beings residing in East Africa[J].J Immunol,1952,69(2):223-234.
[3]de Oliveira WK,Carmo EH,Henriques CM,et al.Zika Virus Infection and Associated Neurologic Disorders in Brazil[J].NEngl J Med,2017,376(16):1591-1593.
[4]Duffy MR,Chen TH,Hancock WT,et al.Zika virus outbreak on Yap Island,Federated States of Micronesia[J].N Engl JMed,2009,360(24):2536-2543.
[5]Heukelbach J,Alencar CH,Kelvin AA,et al.Zika virus outbreak in Brazil[J].J Infect Dev Ctries,2016,10(2):116-120.
[6]Metsky HC,Matranga CB,Wohl S,et al.Zika virus evolution and spread in the Americas[J].Nature,2017,546(7658):411-415.
[7]Liao Y,Zeng ZL,Hu GL,et al.Survey of the first case of Zika virus disease in the mainland of China[J].Zhonghua Liu Xing Bing Xue Za Zhi,2016,37(5):593-596.
[8]Zhang Y,Chen W,Wong G,et al.Highly diversified Zika viruses imported to China,2016[J].Protein Cell,2016,7(6):461-464.
[9]Kraemer MU,Sinka ME,Duda KA,et al.The global distribution of the arbovirus vectors Aedes aegypti and Aedes albopictus[J].Elife,2015,4:e8347.
[10]Zhang S,He GM.Studies on the susceptibility among geographic strains of Aedes albopictus in China to infection with dengue viruses[J].Zhonghua Liu Xing Bing Xue Za Zhi,1989,10(6):348-351.
[11]Gubler DJ,Rosen L.Variation among geographic strains of Aedes albopictus in susceptibility to infection with dengue viruses[J].Am J Trop Med Hyg,1976,25(2):318-325.
[12]Chouin-Carneiro T,Vega-Rua A,Vazeille M,et al.Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus[J].PLoS Negl Trop Dis,2016,10(3):e4543.
[13]Wong PS,Li MZ,Chong CS,et al.Aedes(Stegomyia)albopictus(Skuse):a potential vector of Zika virus in Singapore[J].PLoS Negl Trop Dis,2013,7(8):e2348.2019 3 19 3 J Trop MedMar.2019Vol.19No.3
[14]Mohanty I,Rath A,Mahapatra N,et al.Wolbachia:Abiological control strategy against arboviral diseases[J].J Vector Borne Dis,2016,53(3):199-207.
[15]Blagrove MS,Arias-Goeta C,Failloux AB,et al.Wolbachia strain w Mel induces cytoplasmic incompatibility and blocks dengue transmission in Aedes albopictus[J].Proc Natl Acad Sci USA,2012,109(1):255-260.
[16]Lu P,Bian G,Pan X,et al.Wolbachia induces densitydependent inhibition to dengue virus in mosquito cells[J].PLoSNegl Trop Dis,2012,6(7):e1754.
[17]徐晓晗,李永军,郑小英.沃尔巴克氏体w Pip诱导白纹伊蚊胞质不相容的研究[J].热带医学杂志,2017,17(4):461-464.
[18]Paupy C,Delatte H,Bagny L,et al.Aedes albopictus,an arbovirus vector:from the darkness to the light[J].Microbes Infect,2009,11(14-15):1177-1185.
[19]潘晓玲,刘起勇,奚志勇.基于昆虫共生菌沃尔巴克氏体的蚊媒和蚊媒病控制研究进展[J].中国媒介生物学及控制杂志,2014,25(1):1-7.
[20]Mousson L,Zouache K,Arias-Goeta C,et al.The native Wolbachia symbionts limit transmission of dengue virus in Aedes albopictus[J].PLoS Negl Trop Dis,2012,6(12):e1989.
[21]Moretti R,Yen PS,Houe V,et al.Combining Wolbachiainduced sterility and virus protection to fight Aedes albopictusborne viruses[J].PLoS Negl Trop Dis,2018,12(7):e6626.
[2]Smithburn KC.Neutralizing antibodies against certain recently isolated viruses in the sera of human beings residing in East Africa[J].J Immunol,1952,69(2):223-234.
[3]de Oliveira WK,Carmo EH,Henriques CM,et al.Zika Virus Infection and Associated Neurologic Disorders in Brazil[J].NEngl J Med,2017,376(16):1591-1593.
[4]Duffy MR,Chen TH,Hancock WT,et al.Zika virus outbreak on Yap Island,Federated States of Micronesia[J].N Engl JMed,2009,360(24):2536-2543.
[5]Heukelbach J,Alencar CH,Kelvin AA,et al.Zika virus outbreak in Brazil[J].J Infect Dev Ctries,2016,10(2):116-120.
[6]Metsky HC,Matranga CB,Wohl S,et al.Zika virus evolution and spread in the Americas[J].Nature,2017,546(7658):411-415.
[7]Liao Y,Zeng ZL,Hu GL,et al.Survey of the first case of Zika virus disease in the mainland of China[J].Zhonghua Liu Xing Bing Xue Za Zhi,2016,37(5):593-596.
[8]Zhang Y,Chen W,Wong G,et al.Highly diversified Zika viruses imported to China,2016[J].Protein Cell,2016,7(6):461-464.
[9]Kraemer MU,Sinka ME,Duda KA,et al.The global distribution of the arbovirus vectors Aedes aegypti and Aedes albopictus[J].Elife,2015,4:e8347.
[10]Zhang S,He GM.Studies on the susceptibility among geographic strains of Aedes albopictus in China to infection with dengue viruses[J].Zhonghua Liu Xing Bing Xue Za Zhi,1989,10(6):348-351.
[11]Gubler DJ,Rosen L.Variation among geographic strains of Aedes albopictus in susceptibility to infection with dengue viruses[J].Am J Trop Med Hyg,1976,25(2):318-325.
[12]Chouin-Carneiro T,Vega-Rua A,Vazeille M,et al.Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus[J].PLoS Negl Trop Dis,2016,10(3):e4543.
[13]Wong PS,Li MZ,Chong CS,et al.Aedes(Stegomyia)albopictus(Skuse):a potential vector of Zika virus in Singapore[J].PLoS Negl Trop Dis,2013,7(8):e2348.2019 3 19 3 J Trop MedMar.2019Vol.19No.3
[14]Mohanty I,Rath A,Mahapatra N,et al.Wolbachia:Abiological control strategy against arboviral diseases[J].J Vector Borne Dis,2016,53(3):199-207.
[15]Blagrove MS,Arias-Goeta C,Failloux AB,et al.Wolbachia strain w Mel induces cytoplasmic incompatibility and blocks dengue transmission in Aedes albopictus[J].Proc Natl Acad Sci USA,2012,109(1):255-260.
[16]Lu P,Bian G,Pan X,et al.Wolbachia induces densitydependent inhibition to dengue virus in mosquito cells[J].PLoSNegl Trop Dis,2012,6(7):e1754.
[17]徐晓晗,李永军,郑小英.沃尔巴克氏体w Pip诱导白纹伊蚊胞质不相容的研究[J].热带医学杂志,2017,17(4):461-464.
[18]Paupy C,Delatte H,Bagny L,et al.Aedes albopictus,an arbovirus vector:from the darkness to the light[J].Microbes Infect,2009,11(14-15):1177-1185.
[19]潘晓玲,刘起勇,奚志勇.基于昆虫共生菌沃尔巴克氏体的蚊媒和蚊媒病控制研究进展[J].中国媒介生物学及控制杂志,2014,25(1):1-7.
[20]Mousson L,Zouache K,Arias-Goeta C,et al.The native Wolbachia symbionts limit transmission of dengue virus in Aedes albopictus[J].PLoS Negl Trop Dis,2012,6(12):e1989.
[21]Moretti R,Yen PS,Houe V,et al.Combining Wolbachiainduced sterility and virus protection to fight Aedes albopictusborne viruses[J].PLoS Negl Trop Dis,2018,12(7):e6626.