苏打盐碱胁迫对猴樟幼苗渗透调节的影响研究
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摘要
为研究猴樟幼苗耐盐碱胁迫的能力,以1年生猴樟幼苗为材料,采用0、50mmol·L~(-1)、100mmol·L~(-1)、300mmol·L~(-1)的Na_2CO_3和NaHCO_3(摩尔比Na_2CO_3∶NaHCO_3=1∶1)混合溶液进行处理,测定其叶片的丙二醛、可溶性糖、脯氨酸及可溶性蛋白的含量。结果表明,处理7d时,不同苏打盐碱胁迫处理均提高了猴樟幼苗叶片的脯氨酸、可溶性糖和丙二醛的含量,50mmol/L量,但300mmol/L的胁迫处理则降低了可溶性蛋白含量;随着培养时间的延长,50mmol/L和100mmol/L的胁迫处理的脯氨酸、可溶性糖、可溶性蛋白和丙二醛的含量呈先升高再下降的趋势,而300mmol/L的胁迫处理的脯氨酸、可溶性糖和可溶性蛋白的含量呈下降趋势,丙二醛含量呈上升趋势。28d时,50mmol/L胁迫处理的脯氨酸、可溶性糖和可溶性蛋白的含量与对照差异不显著,而100mmol/L处理显著高于对照,300mmol/L处理均显著低于对照。50mmol/L和100mmol/L处理的丙二醛含量与对照无明显差异,而300mmol/L处理的丙二醛含量明显高于其他处理。表明猴樟幼苗能耐受100mmol/L苏打盐碱胁迫处理。
The annually Cinnamomum bodinieri seedlings were used as material,treated with a mixture of 0,50 mmol·L~(-1),100 mmol·L~(-1),300 mmol·L~(-1) Na_2CO_3 and Na HCO(3 Molar ratio Na_2CO_3∶NaHCO_3= 1∶1),to study the ability of resistance to saline-alkali stress. The content of malonaldehyde,soluble sugar content,proline content and soluble pro-tein content in leaves of Cinnamomum bodinieri seedlings were determined. The results showed that the contents ofproline,soluble sugar and malonaldehyde in the leaves of Cinnamomum bodinieri seedlings were increased by different soda saline-alkali stress treatment under the treatment of 7 d,and the content of soluble protein was increased by50 mmol/L and 100 mmol/L soda saline-alkali stress treatment. However,the soluble protein content were reduced by300 mmol/L soda saline-alkali stress treatment;The proline content,soluble sugar content,soluble protein contentand malondialdehyde content treated with 50 mmol/L and 100 mmol/L soda saline-alkali stress increased and then de-creased with increasing culture time. The proline content,soluble sugar content and soluble protein content treatedwith 300 mmol/L soda saline-alkali stress showed a decreasing trend,and the malonaldehyde content showed an in-creasing trend. At 28 d,the contents of proline,soluble sugar and soluble protein treated with 50 mmol/L soda saline-alkali stress were not significantly different from those of control,while those of treated with 100 mmol/L was signifi-cantly higher than the control,and those of treated with 300 mmol/L was significantly lower than the control. Themalonaldehyde content of 50 mmol/L and 100 mmol/L was not significantly different from the control,while the malo-naldehyde content of 300 mmol/L was significantly higher than other treatments. Conclusion:Cinnamomum bodinieri-seedlings can tolerate 100 mmol/L soda alkali stress.
The annually Cinnamomum bodinieri seedlings were used as material,treated with a mixture of 0,50 mmol·L~(-1),100 mmol·L~(-1),300 mmol·L~(-1) Na_2CO_3 and Na HCO(3 Molar ratio Na_2CO_3∶NaHCO_3= 1∶1),to study the ability of resistance to saline-alkali stress. The content of malonaldehyde,soluble sugar content,proline content and soluble pro-tein content in leaves of Cinnamomum bodinieri seedlings were determined. The results showed that the contents ofproline,soluble sugar and malonaldehyde in the leaves of Cinnamomum bodinieri seedlings were increased by different soda saline-alkali stress treatment under the treatment of 7 d,and the content of soluble protein was increased by50 mmol/L and 100 mmol/L soda saline-alkali stress treatment. However,the soluble protein content were reduced by300 mmol/L soda saline-alkali stress treatment;The proline content,soluble sugar content,soluble protein contentand malondialdehyde content treated with 50 mmol/L and 100 mmol/L soda saline-alkali stress increased and then de-creased with increasing culture time. The proline content,soluble sugar content and soluble protein content treatedwith 300 mmol/L soda saline-alkali stress showed a decreasing trend,and the malonaldehyde content showed an in-creasing trend. At 28 d,the contents of proline,soluble sugar and soluble protein treated with 50 mmol/L soda saline-alkali stress were not significantly different from those of control,while those of treated with 100 mmol/L was signifi-cantly higher than the control,and those of treated with 300 mmol/L was significantly lower than the control. Themalonaldehyde content of 50 mmol/L and 100 mmol/L was not significantly different from the control,while the malo-naldehyde content of 300 mmol/L was significantly higher than other treatments. Conclusion:Cinnamomum bodinieri-seedlings can tolerate 100 mmol/L soda alkali stress.
引文
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[16]颜宏,赵伟,尹尚军,等.烟草对不同盐碱胁迫的生理响应[J].草业学报,2006,15(6):49-55.
[17]段德玉,刘小京,李存桢,等.N素营养对NaCl胁迫下盐地碱蓬幼苗生长及渗透调节物质变化的影响[J].草业学报,2005,14(1):63-68.
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[19]谢国生,朱伯华,彭旭辉,等.水稻苗期对不同pH值下NaCI和NaHC03胁迫响应的比较[J].华中农业大学学报,2005,24(002):121-124.
[2]仇明华,姚祖凤,陈立义.湘西猴樟油主要化学成份分离研究[J].吉首大学学报(自然科学),1990,11(6):81-84.
[3]韦小丽,熊忠华.香樟和猴樟1年生播种苗的生长发育规律[J].山地农业生物学报,2005,24(3):205-208.
[4]王娈,韦小丽,张怡.不同地理种源猴樟苗期的光合特性与蒸腾特性[J].贵州农业科学,2011,39(6):39-42.
[5]李升星,邓志平,李桂盛,等.猴樟与香樟苗期生长情况调查与分析[J].江西林业科技,2012,4:23-25.
[6]宋芳琳,张苗苗,苏金乐,等.自然降温过程中猴樟和香樟幼苗的抗寒性比较[J].西部林业科学,2012,41(6):48-52.
[7]韩浩章,张颖,李素华,等.猴樟无土栽培营养液配方选择研究[J].安徽农学通报,2017,23(11):115-117.
[8]夏永久,刘好霞.宿迁市宿城区园林土壤养分状况初析[J].安徽农学通报,2011,17(23):112-113.
[9]刘建新,王金成,王瑞娟,等.混合盐碱胁迫对燕麦幼苗矿质离子吸收和光合特性的影响[J].干旱地区农业研究,2017,35(1):178-185.
[10]张丽华,王晓立,王梦秋,等.苏打盐碱胁迫对香樟幼苗光合特性的影响[J].北方园艺,2012(23):91-93.
[11]陈超燕,刘洪剑,束庆龙,等.影响市区樟树黄化病的主要因素研究[J].林业科学研究,2008,21(5):625-629.
[12]许惠.生理黄化香樟的叶片养分特征研究[J].安徽农学通报,2009,15(3):137-139.
[13]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.
[14]王洋.NaHCO3模拟盐碱混合胁迫下野生大豆转录组研究[D].哈尔滨:东北农业大学,2014.
[15]陶晶,陈世刚,秦彩云.盐碱胁迫对杨树品种丙二醛及保护酶活性的影响[J].东北林业大学学报,2005,33(3):13-I5.
[16]颜宏,赵伟,尹尚军,等.烟草对不同盐碱胁迫的生理响应[J].草业学报,2006,15(6):49-55.
[17]段德玉,刘小京,李存桢,等.N素营养对NaCl胁迫下盐地碱蓬幼苗生长及渗透调节物质变化的影响[J].草业学报,2005,14(1):63-68.
[18]辛承松,董合忠,棉花盐害与耐盐性的生理和分子机理研究进展[[J].棉花学报,2005,17(5):309-313.
[19]谢国生,朱伯华,彭旭辉,等.水稻苗期对不同pH值下NaCI和NaHC03胁迫响应的比较[J].华中农业大学学报,2005,24(002):121-124.