加味温胆汤对抑郁模型大鼠胃肠动力的影响
|
摘要
[目的]探讨加味温胆汤对抑郁模型大鼠胃肠动力的影响。[方法]将36只SD大鼠随机分为正常组、模型组及加味温胆汤组,每组12只,应用孤养加慢性不可预见性应激方法造模,采用胃排空率检测大鼠胃动力、小肠推进率检测大鼠肠动力、透射电镜检测大鼠胃肠组织的超微结构形态结构。[结果]造模21 d后,与空白组比较,模型组、加味温胆汤组得分低于空白组,差异有统计学意义(P<0.01);给药28 d后,与正常组比较,模型组得分低于正常组,差异有统计学意义(P<0.01);与模型组比较,加味温胆汤组得分高于模型组,差异有统计学意义(P<0.01)。21 d慢性应激后,与空白组比较,其余两组糖水偏爱率显著降低,差异有统计学意义(P<0.01);药物干预4周后,与模型组比较,加味温胆汤组大鼠糖水偏爱度显著增加,差异有统计学意义(P<0.01)。与空白组比较,模型组的胃排空率、小肠推进率显著降低(P<0.01);与模型组比较,加味温胆汤组的胃排空率、小肠推进率增加(P<0.05)。透射电镜结果显示,与空白组比较,模型组大鼠胃主细胞胞膜结构不完整,核严重固缩、核质边移,胞质中部分细胞器出现崩解,线粒体排列不均匀,粗面内质网扩张,电镜下超微形态变化较明显;与模型组比较,加味温胆汤组大鼠胃主细胞上述表现明显减轻。与空白组比较,模型组大鼠结肠黏膜细胞处于崩解状态,损伤严重;与模型组比较,加味温胆汤组的大鼠结肠组织紧密连接较好,接近正常组。[结论]加味温胆汤能够改善抑郁模型大鼠的抑郁状态,增强抑郁症大鼠胃肠动力,改善胃肠组织超微形态结构,为加味温胆汤在临床治疗抑郁症、改善胃肠动力提供了实验支持。
[Objective] To investigate the effect of modified Wendan Decoction on gastrointestinal motility in rats with depression.[Methods] Thirty-six SD rats were randomly divided into normal group,model group and modified Wendan Decoction group,with twelve rats in each group. The model was established by using the solitary and chronic unpredictable stress method. The gastric motility and intestinal propulsion rate were used to detect the intestinal motility of rats. The ultrastructure of gastrointestinal tissue was detected by transmission electron microscopy. [Results] After 21 days,compared with the blank group,the scores of model group,modified Wendan Decoction group were lower than those of the blank group,with statistical significance(P<0.01);28 days after administration,the scores of model group were lower than those of the normal group,with statistical significance(P<0.01);Compared with the model group,the score of modified Wendan Decoction group was higher than that of the model group,with statistical significance(P<0.05).There was no significant difference in activity and preference for sugar and water among the groups before modeling. After 21 days of chronic stress,compared with the blank group,the sugar-water preference rate of the other two groups decreased significantly(P<0.01);after 4 weeks of drug intervention,compared with the model group,the sugar-water preference of rats in modified Wendan Decoction group increased significantly(P<0.01).Compared with the blank group,the gastric emptying rate and small intestinal propulsion rate of the model group were significantly lower than those of the blank group(P<0.01);compared with the model group,the gastric emptying rate and small intestinal propulsion rate of the modified Wendan Decoction group were increased(P <0.05). The results of transmission electron microscopy showed that compared with the blank group,the membrane structure of the gastric main cells in the model group was incomplete,the nucleus was severely condensed,the nucleoplasm was shifted,some organelles in the cytoplasm was collapsed,and the mitochondria were unevenly arranged. The rough of endoplasmic reticulum was expanded,and the ultrastructural changes were more obvious under electron microscope;compared with the model group,the expression of gastric main cells in modified Wendan Decoction group and fluoxetine group was obviously relieved. Compared with the blank group,the disintegration state of colonic mucosal cells in the model group was severely damaged. Compared with the model group,the colonic tissue of the rats in the modified Wendan Decoction group was tightly connected and close to the normal group. [Conclusion] Modified Wendan Decoction can improve the depressive state of depression model rats,enhance gastrointestinal motility and improve the ultrastructure of gastrointestinal tissue in depressive rats,It provides experimental support for modified Wendan Decoction in the treatment of depression to improve gastrointestinal motility.
[Objective] To investigate the effect of modified Wendan Decoction on gastrointestinal motility in rats with depression.[Methods] Thirty-six SD rats were randomly divided into normal group,model group and modified Wendan Decoction group,with twelve rats in each group. The model was established by using the solitary and chronic unpredictable stress method. The gastric motility and intestinal propulsion rate were used to detect the intestinal motility of rats. The ultrastructure of gastrointestinal tissue was detected by transmission electron microscopy. [Results] After 21 days,compared with the blank group,the scores of model group,modified Wendan Decoction group were lower than those of the blank group,with statistical significance(P<0.01);28 days after administration,the scores of model group were lower than those of the normal group,with statistical significance(P<0.01);Compared with the model group,the score of modified Wendan Decoction group was higher than that of the model group,with statistical significance(P<0.05).There was no significant difference in activity and preference for sugar and water among the groups before modeling. After 21 days of chronic stress,compared with the blank group,the sugar-water preference rate of the other two groups decreased significantly(P<0.01);after 4 weeks of drug intervention,compared with the model group,the sugar-water preference of rats in modified Wendan Decoction group increased significantly(P<0.01).Compared with the blank group,the gastric emptying rate and small intestinal propulsion rate of the model group were significantly lower than those of the blank group(P<0.01);compared with the model group,the gastric emptying rate and small intestinal propulsion rate of the modified Wendan Decoction group were increased(P <0.05). The results of transmission electron microscopy showed that compared with the blank group,the membrane structure of the gastric main cells in the model group was incomplete,the nucleus was severely condensed,the nucleoplasm was shifted,some organelles in the cytoplasm was collapsed,and the mitochondria were unevenly arranged. The rough of endoplasmic reticulum was expanded,and the ultrastructural changes were more obvious under electron microscope;compared with the model group,the expression of gastric main cells in modified Wendan Decoction group and fluoxetine group was obviously relieved. Compared with the blank group,the disintegration state of colonic mucosal cells in the model group was severely damaged. Compared with the model group,the colonic tissue of the rats in the modified Wendan Decoction group was tightly connected and close to the normal group. [Conclusion] Modified Wendan Decoction can improve the depressive state of depression model rats,enhance gastrointestinal motility and improve the ultrastructure of gastrointestinal tissue in depressive rats,It provides experimental support for modified Wendan Decoction in the treatment of depression to improve gastrointestinal motility.
引文
[1]Tack J,Lee KJ.Pathophysiology and treatment of functiona dyspepsia[J].J Clin Gastroenterol,2005,39(5):211-216.
[2]谢慧臣.加味四逆散对慢性身心应激模型大鼠胃肠结构及功能的影响[D].武汉:湖北中医药大学,2013.
[3]汤久慧,张丽萍,吴沛然,等.加味温胆汤与氟西汀治疗抑郁症的临床对照研究[J].环球中医药,2013,6(4):253-257.
[4]吴沛然,汤久慧,张丽萍,等.加味温胆汤对抑郁模型大鼠海马SS、SP、NPY、CRH含量的影响[J].中华针灸电子杂志,2015,4(6):259-262.
[5]张丽萍,夏猛,谈驰.解郁1号对抑郁模型大鼠血浆NPY、SP、SS含量的影响[J].福建中医学院学报,2009,19(2):19-21.
[6]邢建峰,封卫毅,侯家玉.小鼠胃排空及小肠推进实验方法的探讨[J].北京中医药大学学报,2003,45(4):50-52.
[7]Porsolt RD.Animal Models of Depression:Utility for Transgenic Research[J].Rev Neurosci,2000,11(1):53-58.
[8]蔡思琪,李沅衡,黎嘉,等.鼠类行为学实验方法研究进展[J].医学综述,2018,24(5):916-920.
[9]Lee HT,Kim BJ.Trimebutine as a modulator of gastrointestina motility[J].Arch Pharm Res,2011,34(6):86l-864.
[10]Zhang M,Leung FP,Huang Y,et al.Increased colonic motility in rat model of irritable bowel syndrome is associated with upregulation of L-type calcium channels in colonic smooth muscl cells[J].Neurogastmenteml Motil,2010,22(5):162-170.
[2]谢慧臣.加味四逆散对慢性身心应激模型大鼠胃肠结构及功能的影响[D].武汉:湖北中医药大学,2013.
[3]汤久慧,张丽萍,吴沛然,等.加味温胆汤与氟西汀治疗抑郁症的临床对照研究[J].环球中医药,2013,6(4):253-257.
[4]吴沛然,汤久慧,张丽萍,等.加味温胆汤对抑郁模型大鼠海马SS、SP、NPY、CRH含量的影响[J].中华针灸电子杂志,2015,4(6):259-262.
[5]张丽萍,夏猛,谈驰.解郁1号对抑郁模型大鼠血浆NPY、SP、SS含量的影响[J].福建中医学院学报,2009,19(2):19-21.
[6]邢建峰,封卫毅,侯家玉.小鼠胃排空及小肠推进实验方法的探讨[J].北京中医药大学学报,2003,45(4):50-52.
[7]Porsolt RD.Animal Models of Depression:Utility for Transgenic Research[J].Rev Neurosci,2000,11(1):53-58.
[8]蔡思琪,李沅衡,黎嘉,等.鼠类行为学实验方法研究进展[J].医学综述,2018,24(5):916-920.
[9]Lee HT,Kim BJ.Trimebutine as a modulator of gastrointestina motility[J].Arch Pharm Res,2011,34(6):86l-864.
[10]Zhang M,Leung FP,Huang Y,et al.Increased colonic motility in rat model of irritable bowel syndrome is associated with upregulation of L-type calcium channels in colonic smooth muscl cells[J].Neurogastmenteml Motil,2010,22(5):162-170.