冬凌草甲素对急性哮喘小鼠Th1细胞/Th2细胞平衡的影响
|
摘要
目的观察冬凌草甲素对卵白蛋白(OVA)诱导小鼠哮喘模型Th1细胞(Th1)/Th2细胞(Th2)平衡的影响。方法按照体重将Balbc雌性小鼠随机分为4组:正常组、模型组和低、高2个剂量实验组,每组10只。建立OVA诱导急性哮喘模型。造模成功后,实验组腹腔注射冬凌草甲素20,40 mg·kg~(-1),正常组与模型组予0. 9%Na Cl,1次/天,连续14 d。以气道高反应仪器检测小鼠气道高反应,用酶联吸附法法检测血清免疫球蛋白E(Ig E)和白细胞介素-4(IL-4)水平,以流式细胞仪检测脾Th1、Th2细胞比例。结果模型组和低、高2个剂量实验组在100μg·m L~(-1)乙酰甲胆碱的气道高反应值分别为2. 99±0. 56,2. 72±0. 68和2. 58±0. 43;这3组的血清Ig E水平(OD值)分别为1. 13±0. 17,0. 76±0. 21和0. 59±0. 19;这3组的血清IL-4水平分别为(66. 3±2. 1),(56. 7±2. 5)和(42. 7±2. 5) pg·m L~(-1);这3组的脾中Th1/Th2分别为0. 67±0. 04,0. 99±0. 04和1. 47±0. 06。上述指标,2个剂量实验组与模型组比较,差异均有统计学意义(P <0. 05,P <0. 01)。结论冬凌草甲素能降低血清Ig E水平,调节Th1/Th2分化,从而改善急性哮喘模型炎症反应。
Objective To observe the effect of oridonin on the Th1/Th2 balance induced by ovalbumin( OVA) in a mice model of asthma.Methods Balbc female mice were randomly divided into 4 groups:normal group,model group,and experimental-low,experimental-high groups,each group had 10 mice. The acute asthma model was induced by OVA. After the model was successfully established,the experimental group was injected with oridonin 20,40 mg · kg~(-1),qd for 14 d. The normal group and the model group were given normal saline. The airway hyperresponsiveness of mice was detected by airway hyperresponsiveness instrument. Serum immunoglobulin E( IgE) and interleukin-4( IL-4)were detected by enzyme-linked adsorption assay. The percentage of Th1,Th2 cells in spleen were detected by flow cytometry. Results The airway hyperresponsiveness values when the concentration of methacholine was 100 μg·m L~(-1) in model,experimental-low,experimental-high groups were 2. 99 ± 0. 56,2. 72 ± 0. 68 and 2. 58 ± 0. 43,respectively.The serum IgE levels( OD values) in the three groups were 1. 13 ± 0. 17,0. 76 ± 0. 21 and 0. 59 ± 0. 19,respectively. The serum IL-4 levels in the three groups were( 66. 3 ± 2. 1),( 56. 7 ± 2. 5) and( 42. 7 ± 2. 5)pg·m L~(-1). The Th1/Th2 in spleen of the three groups were 0. 67 ± 0. 04,0. 99 ± 0. 04 and 1. 47 ± 0. 06,respectively.Comparison between experimental-low,experimental-high groups with model group,the differences of the factors were significant( P < 0. 05,P < 0. 01). Conclusion Oridonin can reduce the level of serum IgE and regulate the differentiation of Th1/Th2,improvingthe inflammatory response of acute asthma model.
Objective To observe the effect of oridonin on the Th1/Th2 balance induced by ovalbumin( OVA) in a mice model of asthma.Methods Balbc female mice were randomly divided into 4 groups:normal group,model group,and experimental-low,experimental-high groups,each group had 10 mice. The acute asthma model was induced by OVA. After the model was successfully established,the experimental group was injected with oridonin 20,40 mg · kg~(-1),qd for 14 d. The normal group and the model group were given normal saline. The airway hyperresponsiveness of mice was detected by airway hyperresponsiveness instrument. Serum immunoglobulin E( IgE) and interleukin-4( IL-4)were detected by enzyme-linked adsorption assay. The percentage of Th1,Th2 cells in spleen were detected by flow cytometry. Results The airway hyperresponsiveness values when the concentration of methacholine was 100 μg·m L~(-1) in model,experimental-low,experimental-high groups were 2. 99 ± 0. 56,2. 72 ± 0. 68 and 2. 58 ± 0. 43,respectively.The serum IgE levels( OD values) in the three groups were 1. 13 ± 0. 17,0. 76 ± 0. 21 and 0. 59 ± 0. 19,respectively. The serum IL-4 levels in the three groups were( 66. 3 ± 2. 1),( 56. 7 ± 2. 5) and( 42. 7 ± 2. 5)pg·m L~(-1). The Th1/Th2 in spleen of the three groups were 0. 67 ± 0. 04,0. 99 ± 0. 04 and 1. 47 ± 0. 06,respectively.Comparison between experimental-low,experimental-high groups with model group,the differences of the factors were significant( P < 0. 05,P < 0. 01). Conclusion Oridonin can reduce the level of serum IgE and regulate the differentiation of Th1/Th2,improvingthe inflammatory response of acute asthma model.
引文
[1] HAN Q,LI S,PENG L,et al. Ent-kaurane diterpenoids from isodon rubescens var. lushanensis[J]. Chem Pharm Bull,2003,51(3):269-272.
[2]王兴兰.冬凌草甲素对哮喘小鼠气道炎症及气道重塑的影响[D].贵州遵义:遵义医学院,2017.
[3]陈叶,贺守第,胡智立,等.香菇多糖对卵白蛋白诱导小鼠哮喘模型树突细胞TIM4表达及炎症影响[J].中国临床药理学与治疗学,2018,23(9):998-1002.
[4]李斌恺,赖克方,洪燕华,等.支气管哮喘小鼠气道反应性无创检测方法的建立[J].中华哮喘杂志(电子版),2009,3(4):243-247.
[5]贺守第,倪慧婕,杨平常,等.类风湿关节炎动物性食物过敏原Ig G和Ig E的检测[J].免疫学杂志,2015,31(10):918-920.
[6] GUO W J,QI H,DENG C Y,et al. The roles of IL-2 and IL-10enhance anti-CD45 RBmAb immune inhibition in allograft skin[J].Immunol Res,2015,61(3):250-259.
[2]王兴兰.冬凌草甲素对哮喘小鼠气道炎症及气道重塑的影响[D].贵州遵义:遵义医学院,2017.
[3]陈叶,贺守第,胡智立,等.香菇多糖对卵白蛋白诱导小鼠哮喘模型树突细胞TIM4表达及炎症影响[J].中国临床药理学与治疗学,2018,23(9):998-1002.
[4]李斌恺,赖克方,洪燕华,等.支气管哮喘小鼠气道反应性无创检测方法的建立[J].中华哮喘杂志(电子版),2009,3(4):243-247.
[5]贺守第,倪慧婕,杨平常,等.类风湿关节炎动物性食物过敏原Ig G和Ig E的检测[J].免疫学杂志,2015,31(10):918-920.
[6] GUO W J,QI H,DENG C Y,et al. The roles of IL-2 and IL-10enhance anti-CD45 RBmAb immune inhibition in allograft skin[J].Immunol Res,2015,61(3):250-259.