羧基改性的聚酰胺——胺/纳米羟基磷灰石的制备和表征
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摘要
目的:制备和表征羧基改性的聚酰胺—胺/纳米羟基磷灰石(PAMAM-COOH/n-HAP)复合材料,并探索其合成时的最佳质量比。方法:通过水热合成法制备不同质量比[m(PAMAM-COOH):m(n-HAP)]的PAMAM-COOH/n-HAP复合材料,利用傅里叶变换红外光谱、透射电镜以及热重分析对其化学信息、形貌和PAMAM-COOH结合到纳米羟基磷灰石上的量进行表征。结果:不同质量比[m(PAMAM-COOH):m(n-HAP)]均可成功制备PAMAM-COOH/n-HAP复合材料,且PAMAMCOOH/n-HAP上PAMAM-COOH涂层厚度不一,其中质量比为1∶4时的厚度为(26.648±1.035)nm,明显高于其它各质量比的厚度(P<0.05),质量比为1∶2时厚度最小,仅为(6.620±0.501)nm。结论:成功制备不同质量比的PAMAM-COOH/n-HAP复合材料,且PAMAM-COOH/n-HAP的最佳质量比为1∶4。
Objective:To prepare and character carboxyl modified polyamide-amine dendrimers/nanohydroxyapatite(PAMAM-COOH/n-HAP) composites and explore the optimum mass ratio.Methods:Prepare different mass ratiosm [m(PAMAM-COOH):m(n-HAP)] of PAMAM-COOH/n-HAP by hydrothermal synthesis method.Fourier transform infrared spectroscopy,transmission electron microscopy and thermogravimetric analysis were used to verify their chemical information,morphology and the amount of PAMAM-COOH bound to nano-hydroxyapatite.Results:PAMAM-COOH/n-HAP composites with different mass ratios were successfully prepared,and the thickness of PAMAM-COOH coating on PAMAMCOOH/n-HAP was different.The thickness of PAMAM-COOH coating was 26.648 ±1.035 nm,when the mass ratio was 1∶4.It was significantly higher than that of other mass ratios(P<0.05).When the mass ratio was 1∶2,the thickness was the smallest,which was only 6.620±0.501 nm.Conclusion:The optimum mass ratio of PAMAM-COOH/n-HAP composites is 1∶4.
Objective:To prepare and character carboxyl modified polyamide-amine dendrimers/nanohydroxyapatite(PAMAM-COOH/n-HAP) composites and explore the optimum mass ratio.Methods:Prepare different mass ratiosm [m(PAMAM-COOH):m(n-HAP)] of PAMAM-COOH/n-HAP by hydrothermal synthesis method.Fourier transform infrared spectroscopy,transmission electron microscopy and thermogravimetric analysis were used to verify their chemical information,morphology and the amount of PAMAM-COOH bound to nano-hydroxyapatite.Results:PAMAM-COOH/n-HAP composites with different mass ratios were successfully prepared,and the thickness of PAMAM-COOH coating on PAMAMCOOH/n-HAP was different.The thickness of PAMAM-COOH coating was 26.648 ±1.035 nm,when the mass ratio was 1∶4.It was significantly higher than that of other mass ratios(P<0.05).When the mass ratio was 1∶2,the thickness was the smallest,which was only 6.620±0.501 nm.Conclusion:The optimum mass ratio of PAMAM-COOH/n-HAP composites is 1∶4.
引文
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[2]ROVERI N,BATTISTELLA E,BIANCHI C L,et al.Surface enamel remineralization:biomimetic apatite nanocrystals and fluoride ions different effects[J].Journal of Nanomaterials,2014,2009(6):8.
[3]LV K L,ZHANG J X,MENG X C,et al.Remineralization effect of the nano-HA toothpaste on artificial caries[J].Key Engineering Materials,2007(330-332):267-270.
[4]KIM M Y,KWON H K,CHOI C H,et al.Combined effects of nano-hydroxyapatite and NaF on remineralization of early caries lesion[J].Key Engineering Materials,2007,330-332:1347-1350.
[5]HUANG S`GAO S,CHENG L,et al.Combined effects of nano-hydroxyapatite and Galla chinensis on remineralisation of initial enamel lesion in vitro[J].J Dent,2010,38(10):811-819.
[6]DAAS I,BADR SANDOSMAN E.Comparison between fluoride and nano-hydroxyapatite in remineralizing initial enamel lesion:an in vitro study[J].J Contemp Dent Pract,2018,19(3):306-312.
[7]SVENSON SANDTOMALIA D A.Dendrimers in biomedical applications--reflections on the field[J].Advanced Drug Delivery Reviews,2012,57(15):2106-2129.
[8]CHEN L,LIANG K,LI J,et al.Regeneration of biomimetic hydroxyapatite on etched human enamel by anionic PAMAM template in vitro[J].Archives of Oral Biology,2013,58(8):975-980.
[9]LIN X,XIE F,MA X,et al.Fabrication and characterization of dendrimer-functionalized nano-hydroxyapatite and its application in dentin tubule occlusion[J].Journal of Biomaterials Science Polymer Edition,2017,28(9):1.
[10]WANG R,WANG Q,WANG X,et al.Enhancement of nano-hydroxyapatite bonding to dentin through a collagen/calcium dual affinitive peptide for dentinal tubule occlusion[J].Journal of Biomaterials Applications,2014,29(2):268-277.
[11]WEI J,LIU A,CHEN L,et al.The surface modification of hydroxyapatite nanoparticles by the ring opening polymerization ofγ-Benzyl-L-glutamate N-carboxyanhydride[J].Macromolecular Bioscience,2009,9(7):631-638.
[12]SKINNER J C,PROSSER H J,SCOTT R P,et al.Adhesion of carboxylate cements to hydroxyapatite.I.The effect of the structure of aliphatic carboxylates on their uptake by hydroxyapatite[J].Biomaterials,1986,7(6):438.
[13]BORUM LANDWILSON O C.Surface modification of hydroxyapatite.Part II.Silica[J].Biomaterials,2003,24(21):3681-3688.
[14]LIU Q,DE WIJN J R,DE G K,et al.Surface modification of nano-apatite by grafting organic polymer[J].Biomaterials,1998,19(11-12):1067.
[15]LI J,YANG J,LI J,et al.Bioinspired intrafibrillar mineralization of human dentine by PAMAM dendrimer[J].Biomaterials,2013,34(28):6738-6747.
[16]LIU Q,DE WIJN J R,BAKKER D,et al.Surface modification of hydroxyapatite to introduce interfacial bonding with polyactive TM 70/30in a biodegradable composite[J].Journal of Materials Science:Materials in Medicine,1996,7(9):551-557.
[17]PRAMANIK NANDIMAE T.Fabrication and characterization of dendrimer-functionalized mesoporous hydroxyapatite[J].Langmuir,2012,28(39):14018-14027.