食蟹猴血糖值与季节性气温变化的关系
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摘要
目的检测并分析食蟹猴血糖值一年内季节性变化,考察食蟹猴血糖值在不同季节间是否存在显著差异。同时比较便携式血糖仪与全自动生化分析仪测定值之间差异。方法采集食蟹猴静脉血,利用全自动生化仪及便携式血糖仪检测不同季节食蟹猴血糖值,进行比较分析。结果显示食蟹猴血糖值呈现夏季最低,冬季最高,春秋位于两者间的周期性波动。且夏→秋和冬→春时间段血糖变化幅度(每℃约0.20 mmol/L)大于秋→冬和春→夏的变化幅度(每℃约0.06 mmol/L)。便携式血糖仪与自动生化分析仪之间血糖值差异符合国家标准,通过比较分析便携式血糖仪可以应用于食蟹猴血糖值检测。结论本研究填补食蟹猴血糖相关研究中,季节性温度变化对其影响分析,有利于在以后动物实验中,充分考虑两者的相关性来对数据进行合理分析。
Objective To examine whether the fasting plasma glucose(FPG) of cynomolgus monkeys(Macaca fascicularis) is significantly different between different seasons. The aim of this study was to investigate the correlation between FPG and seasonal temperature variation in a year, and to compare the FPG measured by a portable blood glucose meter with that measured by an automatic biochemical analyzer. Methods Venous blood of monkeys was collected, and blood glucose values of monkeys in different seasons were measured by an automatic biochemical analyzer and portable blood glucose meter. Results FPG levels in cynomolgus monkeys peaked in the winter, reached the lowest level in the summer, and fluctuated within this range during spring and autumn. Changes in FPG levels during the periods winter→spring and summer→autumn were higher than the periods autumn→winter and spring→summer, with the amplitude changes of approximately 0.20 mmol/L per℃ and 0.06 mmol/L per℃, respectively. The differences between FPG measured by portable blood glucose meter and automatic biochemical analyzer were in accordance with national standards, indicating that the portable blood glucose meter can be used to detect FPG in cynomolgus monkeys. Conclusions The results of this study demonstrate the association of FPG with seasonal temperature variation in cynomolgus monkeys and provide a reasonable analysis of the data in future animal experiments.
Objective To examine whether the fasting plasma glucose(FPG) of cynomolgus monkeys(Macaca fascicularis) is significantly different between different seasons. The aim of this study was to investigate the correlation between FPG and seasonal temperature variation in a year, and to compare the FPG measured by a portable blood glucose meter with that measured by an automatic biochemical analyzer. Methods Venous blood of monkeys was collected, and blood glucose values of monkeys in different seasons were measured by an automatic biochemical analyzer and portable blood glucose meter. Results FPG levels in cynomolgus monkeys peaked in the winter, reached the lowest level in the summer, and fluctuated within this range during spring and autumn. Changes in FPG levels during the periods winter→spring and summer→autumn were higher than the periods autumn→winter and spring→summer, with the amplitude changes of approximately 0.20 mmol/L per℃ and 0.06 mmol/L per℃, respectively. The differences between FPG measured by portable blood glucose meter and automatic biochemical analyzer were in accordance with national standards, indicating that the portable blood glucose meter can be used to detect FPG in cynomolgus monkeys. Conclusions The results of this study demonstrate the association of FPG with seasonal temperature variation in cynomolgus monkeys and provide a reasonable analysis of the data in future animal experiments.
引文
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[11] Suarez L,Barrett-Connor E.Seasonal variation in fasting plasma glucose levels in man [J].Diabetologia,1982,22(4):250-253.
[12] Ryu OH,Lee S,Yoo HJ,et al.Seasonal variations in glycemic control of type 2 diabetes in Korean women [J].J Endocrinol Invest,2014,37(6):575-581.
[13] Taeng CL,Brimacombe M,Xie M,et al.Seasonal patterns in monthly hemoglobin A1c values [J].Am J Epidemiol,2005,161(6):565-574.
[14] Kim YJ,Park S,Yi W,et al.Seasonal variation in hemoglobin A1c in Korean patients with type 2 diabetes mellitus [J].J Korean Med Sci,2014,29(4):550-555.
[15] 王训立,周建华,范春梅.季节性变化对雌性恒河猴生殖功能的影响 [J].中国实验动物学报,2002,10(1):21-26.
[16] Ishii H,Suzuki H,Bata T,et al.Seasonal variation of glycemic control in type 2 diabetic patients [J].Diabetes Care,2001,24(8):1503.
[2] 梁斌,吴晓云.非人灵长类糖尿动物模型研究进展 [J].动物学研究,2011,32(1):91-96.
[3] 王艳静,叶华虎,邵军石.猕猴自发性糖尿病动物模型的初步探讨 [J].中国比较医学杂志,2004,14(1):13-15.
[4] 张六一,张玲,王振宇,等.食蟹猴血液学和生化学指标数据背景资料的建立 [J].四川生理科学杂志,2005,27(1):39-40.
[5] 徐传磊,陈艳明,徐志勇,等.建立食蟹猴糖尿病模型的研究 [J].广州中医药大学学报,2009,26(1):91-94.
[6] 郝香芬,万玉玲,李学家,等.食蟹猴的基础血糖值调查 [J].四川动物,2010,30(1):111-114.
[7] Ramsey JJ,Laatsch JL,Kemnitz JW.Age and gender differences in body composition,energy expenditure,and glucoregulation of adult rhesus monkeys [J].J Med Primatol,2003,29(1):11-19.
[8] 文秀丽,孙莹.血糖仪测定毛细血管和静脉血糖的结果比较 [J].辽宁药物与临床,2003,6(1):38-39.
[9] 曾宪成,马璟,杨琛懋,等.食蟹猴血液学及血清生化指标日间节规律变化 [J].中国比较医学杂志,2010,20(6):43-48.
[10] Fahlen M,Oden A,Bjorntorp P,et al.Seasonal influence on insulin secretion in man [J].Clin Sci,1971,41(5):453-458.
[11] Suarez L,Barrett-Connor E.Seasonal variation in fasting plasma glucose levels in man [J].Diabetologia,1982,22(4):250-253.
[12] Ryu OH,Lee S,Yoo HJ,et al.Seasonal variations in glycemic control of type 2 diabetes in Korean women [J].J Endocrinol Invest,2014,37(6):575-581.
[13] Taeng CL,Brimacombe M,Xie M,et al.Seasonal patterns in monthly hemoglobin A1c values [J].Am J Epidemiol,2005,161(6):565-574.
[14] Kim YJ,Park S,Yi W,et al.Seasonal variation in hemoglobin A1c in Korean patients with type 2 diabetes mellitus [J].J Korean Med Sci,2014,29(4):550-555.
[15] 王训立,周建华,范春梅.季节性变化对雌性恒河猴生殖功能的影响 [J].中国实验动物学报,2002,10(1):21-26.
[16] Ishii H,Suzuki H,Bata T,et al.Seasonal variation of glycemic control in type 2 diabetic patients [J].Diabetes Care,2001,24(8):1503.