喀斯特石漠化区植被恢复不同阶段土壤真菌群落组成分析
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摘要
为阐明西南喀斯特石漠化区植被恢复过程土壤真菌群落组成及优势关键类群,采集乔林、灌木林、灌草和草本群落4种不同植被恢复阶段不同小生境中(石面、石沟、石缝)的表层土壤,提取其DNA,利用IlluminaHiSeq平台对真菌ITS区扩增进行高通量测序后注释鉴定,开展植被恢复不同阶段真菌群落组成结构分析。结果表明,36个土壤样品中共获得2 437 541条有效序列,每个样品获得35 911条有效序列,OTU数目为5 437,包含5个门、626个属。乔林、灌木林阶段土壤真菌群落组成相近,由未分类真菌、Geminibasidium、被孢霉属Mortierella、蜡壳耳属Sebacina、青霉属Penicillium、子囊菌门Ascomycota未定属等组成,其中木霉属Trichoderma(LDA=4.11)、青霉属(LDA=4.35)、小蔓毛壳科Herpotrichiellaceae(LDA=4.00)、革菌科Thelephoraceae(LDA=4.10)、未分类真菌(LDA=4.86)为该类型的关键优势真菌类群。灌草过渡阶段与草本群落阶段各成一种类型,灌草过渡阶段包括蜡壳耳属、Geminibasidium、被孢霉属等,其中,蜡壳耳属(LDA=5.30)、Geminibasidium(LDA=4.77)、煤炱目Capnodiales(LDA=4.15)、格孢菌目Pleosporales未定属(LDA=4.28)为该类型的关键优势真菌类群。草本群落阶段由未分类真菌、镰刀菌属Fusarium、子囊菌门、被孢霉属、Archaeorhizomyces等组成,镰刀菌属(LDA=4.78)、Archeaorhizomyces(LDA=4.48)、支顶孢属(LDA=4.22)、肉座菌科(LDA=4.54)、子囊菌门未定属(LDA=4.63)为该类型的关键优势真菌类群。
To clarify the composition of soil fungi community and dominant key group in different stages of vegetation restoration in karst rocky desertification area, the topsoil in different niches under four different vegetation restoration stages of forest,shrubland,brushwood and glassland communities was collected for DNA extraction. The ITS region of fungi was amplified by Illumina HiSeq platform, high throughput sequencing was performed, the species annotations of the fungi. The composition and structure of fungal community in different stages of vegetation restoration was analyzed. The result showed that a total of 2 437 541 effective sequences were obtained from 36 soil samples, and 35 911 effective sequences were obtained for each sample. The number of OTUs was 5 437, including 5 phylum and 626 genus. The composition of soil fungi in forest and shrubland stage was similar,mainly consisting of Geminibasidium, Mortierella, Sebacina, Penicillium, Ascomycota and unclassified fungi. Trichoderma(LDA=4.11), Penicillium(LDA=4.35), Herpotrichiellaceae(LDA=4.00), Thelephoraceae(LDA=4.10), and unclassified fungi(LDA=4.86) were the key dominant fungi groups. The transitional stage of brushwood and grassland community was divided into one type. The transitional stage of brushwood stage included Sebacina, Geminibasidium, Mortierella, et al., among them, Sebacina(LDA=5.30), Geminibasidium(LDA=4.77), Capnodiales(LDA=4.15), Pleosporales(LDA=4.28) were the key dominant fungi groups of this type. Unclassified fungi, Fusarium, Mortierella, Archaeorhizomyces and Ascomycota was mainly concentrated in the grassland stage. Fusarium(LDA=4.78), Archeaorhizomyces(LDA=4.48), Acremonium(LDA=4.22), Hypocreales(LDA=4.63) and Ascomycota(LDA=4.77) were the key dominant fungal groups of this type.
To clarify the composition of soil fungi community and dominant key group in different stages of vegetation restoration in karst rocky desertification area, the topsoil in different niches under four different vegetation restoration stages of forest,shrubland,brushwood and glassland communities was collected for DNA extraction. The ITS region of fungi was amplified by Illumina HiSeq platform, high throughput sequencing was performed, the species annotations of the fungi. The composition and structure of fungal community in different stages of vegetation restoration was analyzed. The result showed that a total of 2 437 541 effective sequences were obtained from 36 soil samples, and 35 911 effective sequences were obtained for each sample. The number of OTUs was 5 437, including 5 phylum and 626 genus. The composition of soil fungi in forest and shrubland stage was similar,mainly consisting of Geminibasidium, Mortierella, Sebacina, Penicillium, Ascomycota and unclassified fungi. Trichoderma(LDA=4.11), Penicillium(LDA=4.35), Herpotrichiellaceae(LDA=4.00), Thelephoraceae(LDA=4.10), and unclassified fungi(LDA=4.86) were the key dominant fungi groups. The transitional stage of brushwood and grassland community was divided into one type. The transitional stage of brushwood stage included Sebacina, Geminibasidium, Mortierella, et al., among them, Sebacina(LDA=5.30), Geminibasidium(LDA=4.77), Capnodiales(LDA=4.15), Pleosporales(LDA=4.28) were the key dominant fungi groups of this type. Unclassified fungi, Fusarium, Mortierella, Archaeorhizomyces and Ascomycota was mainly concentrated in the grassland stage. Fusarium(LDA=4.78), Archeaorhizomyces(LDA=4.48), Acremonium(LDA=4.22), Hypocreales(LDA=4.63) and Ascomycota(LDA=4.77) were the key dominant fungal groups of this type.
引文
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白丽,范席德,王洁莹,等,2018.黄土高原草地次生演替过程中微生物群落对植物群落的响应[J].生态环境学报,27(10):1801-1808.BAI L,FAN X D,WANG J Y,et al.,2018.Responses of microbial communities to plant communities during secondary succession of grassland in loness plateau[J].Ecology and Environmental Sciences,27(10):1801-1808.
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戴雅婷,闫志坚,解继红,等,2017.于高通量测序的两种植被恢复类型根际土壤细菌多样性研究[J].土壤学报,54(3):735-748.DAI Y T,YAN Z J,XIE J H,et al.,2017.Soil Bacteria diversity in rhizosphere under two types of vegetation restoration based on heigh throughpue sequencing[J].Acta Pedologica Sinica,54(3):735-748.
范夫静,黄国勤,宋同清,等,2014.西南峡谷型喀斯特坡地土壤微生物量C、N、P空间变异特征[J].生态学报,34(12):3293-3301.FAN F J,HUANG G Q,SONG T Q,et al.,2014.Spatial heterogeneity of soil microbial biomass carbon,nitrogen,and phosphorus in sloping field in a groge karst region,Southwest China[J].Acta Ecologica Sinica,34(12):3293-3301.
郭成瑾,张丽荣,沈瑞清,等,2017.宁夏境内腾格里沙漠固沙植物根际土壤真菌多样性研究[J].菌物学报,36(5):552-562.GUO C J,ZHANG L R,SHEN R Q,et al.,2017.Diversity of rhizosphere soil fungi in sand-fixation plants in tengger desert of ningxia autonomous region[J].Mycosystema,36(5):552-562.
郭良栋,田春杰,2013.菌根真菌的碳氮循环功能研究进展[J].微生物学通报,40(1):158-171.GUO L D,TIAN C J,2013.Progress of the function of mycorrhizal fungi in the cycle of carbon and nitrogen[J].Microbiology China,40(1):158-171.
胡芳,杜虎,曾馥平,等,2018.典型喀斯特峰丛洼地不同植被恢复对土壤养分含量和微生物多样性的影响[J].生态学报,38(6):2170-2179.HU F,DU H,ZENG F P,et al.,2018.Dynamics of soil nutrient content and microbial diversity following vegetation restoration in a typical karst peak-cluster depression landscape[J].Acta Ecologica Sinica,38(6):2170-2179.
李香真,郭良栋,李家宝,等,2016.中国土壤微生物多样性监测的现状和思考[J].生物多样性,24(11):1240-1248.LI X Z,GUO L D,LI J B,et al.,2016.Soil microbial diversity observation in China:current situation and future consideration[J].Biodiversity Science,24(11):1240-1248.
梁月明,何寻阳,苏以荣,等,2010.喀斯特峰丛洼地植被恢复过程中土壤微生物特性[J].生态学杂志,29(5):917-922.LIANG Y M,HE X Y,SU Y R,et al.,2010.Dynamic changes of soil microbial properties in karst peak-cluster depression area during vegetation restoration[J].Chinese Journal of Ecology,29(5):917-922.
廖洪凯,李娟,龙健,等,2013.贵州喀斯特山区花椒林小生境类型与土壤环境因子的关系[J].农业环境科学学报,32(12):2429-2435.LIAO H K,LI J,LONG J,et al.,2013.Soil Characteristics of different microhabitats of Chinese prickly ash in karst mountain areas of guizhou province[J].Journal of Agro-Environment Science,32(12):2429-2435.
廖洪凯,龙健,李娟,等,2012.西南地区喀斯特干热河谷地带不同植被类型下小生境土壤碳氮分布特征[J].土壤,44(3):421-428.LIAO H K,LONG J,LI J,et al.,2012.Distribution characteristics of soil carbon and nitrogen under different vegetation types in micro-habitats of karst dry-hot valley region of south western China[J].Soils,44(3):421-428.
刘方,王世杰,罗海波,等,2008.喀斯特森林生态系统的小生境及其土壤异质性[J].土壤学报,45(6):1055-1062.LIU F,WANG S J,LUO H B,et al.,2008.Micro-habitats in karst forest ecosystem and variability of soils[J].Acta Pedologica Sinica,45(6):1055-1062.
鲁如坤,1999.土壤农业化学分析方法[M].北京:中国农业科技出版社:146-195.LU R K,1999.Soil argrochemistry analysis protocoes[M].Beijing:China Agriculture Science Press:146-195.
盛茂银,刘洋,熊康宁,2013.中国南方喀斯特石漠化演替过程中土壤理化性质的响应[J].生态学报,33(19):6303-6313.SHENG M Y,LIU Y,XIONG K N,2013.Response of soil physical-chemical properties to rocky desertification succession in south China Karst[J].Acta Ecologica Sinica,33(19):6303-6313.
宋敏,邹冬生,杜虎,等,2013.不同土地利用方式下喀斯特峰丛洼地土壤微生物群落特征[J].应用生态学报,24(9):2471-2478.SONG M,ZOU D S,DU H,et al.,2013.Characteristics of soil microbial populations in depressions between karst hills under different land use patterns[J].Chinese Journal of Applied Ecology,24(9):2471-2478.
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