一株铁皮石斛内生真菌的色素稳定性研究
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摘要
本研究以铁皮石斛体内分离筛选得到一株产橙红色色素的内生真菌X1为研究对象,为明确该菌株的种属及其所产天然色素的稳定性,采用分子生物学的方法,鉴定该菌株归属于Neurospora sp.一种。该菌株所产橙色素在400 nm波长处有最大吸收峰;从温度、pH、光照、氧化剂与还原剂四个单因子,对该株菌进行色素稳定性研究。结果表明该色素在紫外光下放置6 h橙色素光吸收值增大,说明有增色效应;在pH 2～8,20～100℃下不受太大影响;H_20_2和Na_2SO_3对该色素有较强的破坏力;Fe~(3+)、Zn~(2+)、Cu~(2+)、Ca~(2+)和Mn~(2+)对该色素具有保色或增色作用;10%NaCl和15%葡萄糖溶液对色素均有增色效应。该研究对内生真菌分离及真菌色素提取工艺具有一定的理论指导意义。
In this study,an endophytic fungi X1 that produces orange pigment was isolated and screened out from Dendrobium candidum.In order to clarify the species of this strain and the stability of its natural pigments,and the strain was identified as Neurospora sp.based on molecular biology.The orange pigment which produced at this strain has the highest absorption peak at 400 nm.It is show that when the pigment is placed in ultraviolet light for 6 h,the absorption value increased,indicating a color enhancement effect.It is not affected much at pH 2-8 and 20-100 ℃.Furthermore,H_2O_2 and Na_2SO_(3 )have strong destructive power to the pigment though several common additives have no effect on the pigment.In the metalions,Fe~(3+),Zn~(2+),Cu~(2+),Ca~(2+)and Mn~(2+) have color protection or burnish effect on the pigment.Meanwhile,10%NaCl and 15% glucose solution also have a significant hyperchromic effect on it.This study has theoretical and practical importance for the separation of endophytic fungi and extraction process of fungal pigments.
In this study,an endophytic fungi X1 that produces orange pigment was isolated and screened out from Dendrobium candidum.In order to clarify the species of this strain and the stability of its natural pigments,and the strain was identified as Neurospora sp.based on molecular biology.The orange pigment which produced at this strain has the highest absorption peak at 400 nm.It is show that when the pigment is placed in ultraviolet light for 6 h,the absorption value increased,indicating a color enhancement effect.It is not affected much at pH 2-8 and 20-100 ℃.Furthermore,H_2O_2 and Na_2SO_(3 )have strong destructive power to the pigment though several common additives have no effect on the pigment.In the metalions,Fe~(3+),Zn~(2+),Cu~(2+),Ca~(2+)and Mn~(2+) have color protection or burnish effect on the pigment.Meanwhile,10%NaCl and 15% glucose solution also have a significant hyperchromic effect on it.This study has theoretical and practical importance for the separation of endophytic fungi and extraction process of fungal pigments.
引文
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14 Jiang QF,Chen ML,Yuan ZH.Study on extraction and stability of monascus pigment from Red Kojic Rice[J].Chin Condiment(中国调味品),2014,39(9):1-3.
15 Zhang D.Studiy on the fermentation of monacus pigment by monacus and stability of monacus pigment[D].Anhui:Anhui Polytechnic University(安徽工程大学),2014.
16 Wang LJ,Zhang F,He PX.Isolation,identification and red pigment properties of fungi in rhizosphere soil[J].Food Sc and Technol(食品科技),2014,39(4):15-19.
17 Wang B,Xu LG,Yu ZD,et al.Study of producing condition and the stability of a red pigment from Lecanicillium psalliotae Zare & W.Gams[J].J Northwest Forestry Univ(西北林学院学报),2013,28:112-116.
18 Xie LM,Li JR.Study on the extraction of monascus pigment and its stabilization[J].Sci and Tech Food Ind(食品工业科技),2004,3:118-121.
19 Wang HK,Zhang TY,Zhang M.Advnces on taxonomic studies of the genus alternaria[J].J Shandong Agric Univ(山东农业大学学报),2001,32:406-410.
20 Li HY.The preparation of fungal pigments and their dyeing research onproteinfiber fabric[D].Zhejiang:Zhejiang Sci-Tech University(浙江理工大学),2015.
2 Feng YJ,Song M.Endophytic bacteria[J].Chin J Nat(自然杂志),2001,5:249-252.
3 Andrews JH.Biological control in the phylloshere[J].Annu Rev Phytopathol,1992,30:603-635.
4 Gong Y,He WB,Wang SZ,et al.Research progress on endophyte from Dendrobium officinale[J].J Zhejiang Agric Sci(浙江农业科学),2017,58:1372-1375.
5 Wang WY,Li HM,Zhou H,et al.Research advances in the functions to promote endophytic fungi of Dendrobium offifcinale[J].Chin Hortic Abstr(中国园艺文摘),2016,32(11):52-53.
6 Yang HT.Screening and functional characterization of endophytic mycorrhizal fungus in Dendrobium officinale[D].Nanjing:Nanjing Agricultural University(南京农业大学),2015.
7 Ma Z,Tan CC,Zhang C,et al.The relationship between endophytes and host plants:implications for research on geoherbalism[J].J Shanghai Univ Tradit Chin Med(上海中医药大学学报),2015,29(6):4-11.
8 Li Y,Luo PF,Zhou DL,et al.Isolation and identification of endophytic fungi in the root,stem and leaves of Dendrobium nobile lindl[J].J Anhui Agric Sci(安徽农业科学),2012,40:11598-11600.
9 Pei XX,Li YF,Cai DY,et al.Study on the stability of yellow pigments produced by rndnophytic fungus RML6 from Lycium ruthenicum Murr[J].Nat Prod Res Dev(天然产物研究与开发),2018,30(1):10-14.
10 Hu SY,Liu S,Wang SJ,et al.Isolation and identification of a melanin-producing marine fungi and characterization of the pigment[J].Biotechnol Bull(生物技术通报),2017,33:138-143.
11 Chen Q,Wang SJ,Wang HM,et al.Preliminary identification of red pigments producing fungus and extraction condition[J].J Zaozhuang Univ(枣庄学院学报),2017,34(5):91-98.
12 Liu W,Ruan LY,Guan CL,et al.Study on stability of red pigments produced by an ednophytic fungus H1B1 from Dendrobium of cinale[J].Chin Food Additives(中国食品添加剂),2016,7:71-75.
13 Zhang LQ,Liang HB,Ai TS,et al.Characterization of a red pigments producing fungus and study on the stabilty of the red pigments[J].Hubei Agric Sci(湖北农业科学),2015,54:2863-2865.
14 Jiang QF,Chen ML,Yuan ZH.Study on extraction and stability of monascus pigment from Red Kojic Rice[J].Chin Condiment(中国调味品),2014,39(9):1-3.
15 Zhang D.Studiy on the fermentation of monacus pigment by monacus and stability of monacus pigment[D].Anhui:Anhui Polytechnic University(安徽工程大学),2014.
16 Wang LJ,Zhang F,He PX.Isolation,identification and red pigment properties of fungi in rhizosphere soil[J].Food Sc and Technol(食品科技),2014,39(4):15-19.
17 Wang B,Xu LG,Yu ZD,et al.Study of producing condition and the stability of a red pigment from Lecanicillium psalliotae Zare & W.Gams[J].J Northwest Forestry Univ(西北林学院学报),2013,28:112-116.
18 Xie LM,Li JR.Study on the extraction of monascus pigment and its stabilization[J].Sci and Tech Food Ind(食品工业科技),2004,3:118-121.
19 Wang HK,Zhang TY,Zhang M.Advnces on taxonomic studies of the genus alternaria[J].J Shandong Agric Univ(山东农业大学学报),2001,32:406-410.
20 Li HY.The preparation of fungal pigments and their dyeing research onproteinfiber fabric[D].Zhejiang:Zhejiang Sci-Tech University(浙江理工大学),2015.