硫化氢对镉胁迫下大白菜幼苗光合生理的影响
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摘要
为了研究H2S对镉(Cd)胁迫下大白菜[Brassica pekinensis(Lour.)Rupr.]幼苗光合生理的调节作用,通过生理生化实验检测Cd胁迫和H2S对幼苗光合特性指标和Rubp羧化酶(Ribulose-1,5-hisphpsphate carboxylase)活性的影响,利用荧光定量-PCR检测Rubp小亚基编码基因RBCS表达水平的变化。结果表明:5 mmol·L~(-1)Cd胁迫会显著抑制大白菜幼苗的生长,降低叶片叶绿素含量、叶绿素荧光参数(F_v/F_0、F_v/F_m、PI_(abs))和净光合速率(Pn);5μmol·L~(-1)NaHS预处理可以明显缓解Cd胁迫对上述指标的抑制作用,增加Cd胁迫下RBCS的表达量,促进非Cd胁迫下Rubp羧化酶的活性;0.1 mmol·L~(-1)次牛磺酸(H_2S清除剂)与Cd共同处理对幼苗的损伤比单独Cd胁迫更强。以上结果表明,H_2S预处理可以提高大白菜幼苗的光合作用强度,有效缓解Cd胁迫对其造成的损伤,维持Cd胁迫下大白菜幼苗的生长。
To study the function of hydrogen sulfide(H_2S)on the photosynthetic physiology of Chinese cabbage[Brassica pekinensis(Lour.)Rupr.] seedlings under cadmium(Cd)stress, we examined the effects of Cd stress and H_2S on photosynthetic characteristics, ribulose-1,5-hisphpsphate(Rubp)carboxylase activity, and the expression level of RBCSes(small subunits of Rubp)using physiological, biochemical,and real-time quantitative PCR analyses. Seedling growth, chlorophyll content, photosynthetic fluorescence parameters(e.g., F_v/F_0, F_v/F_m,and PI_(abs)), and net photosynthetic rate(Pn)were inhibited considerably under 5 mmol·L~(-1) Cd stress, whereas 5 μmol·L~(-1) NaHS pretreatment alleviated the effects of Cd stress physiologically and up-regulated the mRNA levels of RBCSes under Cd stress. H_2S promoted the activity of seedling Rubp carboxylase without Cd stress. In contrast, the presence of 0.1 mmol·L~(-1) hypotaurine(H_2S scavenger)aggravated the inhibition effects of Cd stress on the seedlings. Thus, H_2S enhanced the photosynthesis of seedlings to alleviate the damage caused by Cd stress and to maintain the growth of Chinese cabbage under Cd stress.
To study the function of hydrogen sulfide(H_2S)on the photosynthetic physiology of Chinese cabbage[Brassica pekinensis(Lour.)Rupr.] seedlings under cadmium(Cd)stress, we examined the effects of Cd stress and H_2S on photosynthetic characteristics, ribulose-1,5-hisphpsphate(Rubp)carboxylase activity, and the expression level of RBCSes(small subunits of Rubp)using physiological, biochemical,and real-time quantitative PCR analyses. Seedling growth, chlorophyll content, photosynthetic fluorescence parameters(e.g., F_v/F_0, F_v/F_m,and PI_(abs)), and net photosynthetic rate(Pn)were inhibited considerably under 5 mmol·L~(-1) Cd stress, whereas 5 μmol·L~(-1) NaHS pretreatment alleviated the effects of Cd stress physiologically and up-regulated the mRNA levels of RBCSes under Cd stress. H_2S promoted the activity of seedling Rubp carboxylase without Cd stress. In contrast, the presence of 0.1 mmol·L~(-1) hypotaurine(H_2S scavenger)aggravated the inhibition effects of Cd stress on the seedlings. Thus, H_2S enhanced the photosynthesis of seedlings to alleviate the damage caused by Cd stress and to maintain the growth of Chinese cabbage under Cd stress.
引文
[1]Wei B G,Yang L S.A review of heavy metal contaminations in urban soils,urban road dusts and agricultural soils from China[J].Microchemical Journal,2010,94(2):99-107.
[2]Yilmaz D D,Parlak K U.Changes in proline accumulation and antioxidative enzyme activities in Groenlandia densa under cadmium stress[J].Ecological Indicators,2011,11(2):417-423.
[3]Gogorcena Y,Larbi A,Andaluz S,et al.Effects of cadmium on cork oak(Quercus suber L.)plants grown in hydroponics[J].Tree Physiology,2011,31(12):1401-1412.
[4]Baszynski T,Wajda L,Krol M,et al.Photosynthetic activities of cadmium-treated tomato plants[J].Physiologia Plantarum,2010,48(3):365-370.
[5]方慧慧,裴雁曦.H2S信号在植物抵御重金属胁迫过程中的作用[J].中国细胞生物学学报,2017,36(6):139-145.FANG Hui-hui,PEI Yan-xi.Physiological functions of gasotransmitter hydrogen sulfide in plant defense against heavy metals stress[J].Chinese Journal of Cell Biology,2017,36(6):139-145.
[6]裴雁曦.植物中的气体信号分子硫化氢:无香而立,其臭如兰[J].中国生物化学与分子生物学,2016,32(7):721-733.PEI Yan-xi.Gasotransmitter hydrogen sulfide in plants:Stinking to high heaven,but refreshing to fine life[J].Chinese Journal of Biochemistry and Molecular Biology,2016,32(7):721-733.
[7]Dawood M,Cao F,Jahangir M M,et al.Alleviation of aluminum toxicity by hydrogen sulfide is related to elevated ATPase,and suppressed aluminum uptake and oxidative stress in barley[J].Journal of Hazardous Materials,2012,209/210:121-128.
[8]Sun J,Wang R,Zhang X,et al.Hydrogen sulfide alleviates cadmium toxicity through regulations of cadmium transport across the plasma and vacuolar membranes in Populus euphratica cells[J].Plant Physiology and Biochemistry,2013,65:67-74.
[9]Chen J,Wang W H,Wu F H,et al.Hydrogen sulfide alleviates aluminum toxicity in barley seedlings[J].Plant and Soil,2013,362(1/2):301-318.
[10]Chen X,Wang J,Shi Y,et al.Effects of cadmium on growth and photosynthetic activities in pakchoi and mustard[J].Botanical Studies,2011,52(1):41-46.
[11]Dean C,Pichersky E,Dunsmuir P.Structure,evolution,and regulation of RbcS genes in higher plants[J].Annual Review of Plant Physiology and Plant Molecular Biology,1989,40(1):415-439.
[12]Sclmeider G,Lindqvist Y,Branden C I.Rubisco:Structure and mechanism[J].Annual Review of Biophysics and Biomolecular Structure,1992,21:119-143.
[13]Spreitzer R J.Role of the small subunit in ribulose-1,5-bisphosphate carboxylase/oxygenase[J].Archives of Biochemistry and Biophysics,2003,414(2):141-149.
[14]王维光,李立人.菠菜二磷酸核酮糖(RuBP)羧化酶简化提纯研究[J].植物生理学报,1980(3):257-262.WANG Wei-guang,LI Li-ren.Simplified purification of spinosa diphosphate ketone(RuBP)carboxylase[J].Plant Physiology Journal,1980(3):257-262.
[15]金竹萍,裴雁曦.植物H2S气体信号分子的生理功能研究进展[J].中国细胞生物学学报,2013,35(6):880-888.JIN Zhu-ping,PEI Yan-xi.Research progress on hydrogen sulfide signaling in plants[J].Chinese Journal of Cell Biology,2013,35(6):880-888.
[16]Chen J,Wu F H,Wang W H,et al.Hydrogen sulphide enhances photosynthesis through promoting chloroplast biogenesis,photosynthetic enzyme expression,and thiol redox modification in Spinacia oleracea seedlings[J].Journal of Experimental Botany,2011,62(13):4481-4493.
[17]冯世静,杨途熙,张艳军,等.镉胁迫对杨树光合特性的影响[J].农业环境科学学报,2013,32(3):539-547.FENG Shi-jing,YANG Tu-xi,ZHANG Yan-jun,et al.Effects of cadmium on photosynthetic gas exchange and chlorophyll fluorescence of two species of Poplar[J].Journal of Agro-Environment Science,2013,32(3):539-547.
[18]王鸿蕉,张丽萍,刘志强,等.外源硫化氢对冷胁迫下白菜幼苗生长和光合作用的影响[J].西北植物学报,2015,35(4):780-786.WANG Hong-jiao,ZHANG Li-ping,LIU Zhi-qiang,et al.Influence of H2S on growth and photosynthesis of Brassica rapa var.pekinensis under chilling stress[J].Acta Botanica Boreali-Occidentalia Sinica,10163852015,35(4):780-786.
[19]孙丽敏,金竹萍,钱嘉航,等.干旱胁迫下H2S信号增强植物叶片的光合作用[J].中国细胞生物学学报,2017,35(12):1583-1591.SUN Li-min,JIN Zhu-ping,QIAN Jia-hang,et al.H2S enhanced photosynthesis in response to drought stress[J].Chinese Journal of Cell Biology,2017,35(12):1583-1591.
[20]Krause G H,Weis E.Chlorophyll fluorescence and photosynthesis:The basics[J].Annual Review of Plant Physiology and Plant Molecular Biology,1991,42(1):313-349.
[21]裴雁曦,贺烽,解梦洁,等.气体信号分子H2S和NO对冷胁迫下大白菜幼苗光合作用的影响[J].山西大学学报(自然科学版),2017,40(3):596-601.PEI Yan-xi,HE Feng,XIE Meng-jie,et al.Effects of gasotransmitters NO and H2S on photosynthesis of Chinese cabbage seedlings under chilling stress[J].Journal of Shanxi University(Natural Science Edition),2017,40(3):596-601.
[22]Denise S,Lorenzo L,Carlos G,et al.Gasotransmitters and stomatal closure:Is there redundancy,concerted action,or both?[J].Frontiers in Plant Science,2016,7:1-5.
[23]Qiao Z J,Jing T,Liu Z Q,et al.H2S acting as a downstream signaling molecule of SA regulates Cd tolerance in Arabidopsis[J].Plant and Soil,2015,393:137-146.
[24]Mustafa A K,Gadalla M M,Sen N,et al.H2S signals through protein S-sulfhydration[J].Science Signaling,2009,2(96):ra72.
[2]Yilmaz D D,Parlak K U.Changes in proline accumulation and antioxidative enzyme activities in Groenlandia densa under cadmium stress[J].Ecological Indicators,2011,11(2):417-423.
[3]Gogorcena Y,Larbi A,Andaluz S,et al.Effects of cadmium on cork oak(Quercus suber L.)plants grown in hydroponics[J].Tree Physiology,2011,31(12):1401-1412.
[4]Baszynski T,Wajda L,Krol M,et al.Photosynthetic activities of cadmium-treated tomato plants[J].Physiologia Plantarum,2010,48(3):365-370.
[5]方慧慧,裴雁曦.H2S信号在植物抵御重金属胁迫过程中的作用[J].中国细胞生物学学报,2017,36(6):139-145.FANG Hui-hui,PEI Yan-xi.Physiological functions of gasotransmitter hydrogen sulfide in plant defense against heavy metals stress[J].Chinese Journal of Cell Biology,2017,36(6):139-145.
[6]裴雁曦.植物中的气体信号分子硫化氢:无香而立,其臭如兰[J].中国生物化学与分子生物学,2016,32(7):721-733.PEI Yan-xi.Gasotransmitter hydrogen sulfide in plants:Stinking to high heaven,but refreshing to fine life[J].Chinese Journal of Biochemistry and Molecular Biology,2016,32(7):721-733.
[7]Dawood M,Cao F,Jahangir M M,et al.Alleviation of aluminum toxicity by hydrogen sulfide is related to elevated ATPase,and suppressed aluminum uptake and oxidative stress in barley[J].Journal of Hazardous Materials,2012,209/210:121-128.
[8]Sun J,Wang R,Zhang X,et al.Hydrogen sulfide alleviates cadmium toxicity through regulations of cadmium transport across the plasma and vacuolar membranes in Populus euphratica cells[J].Plant Physiology and Biochemistry,2013,65:67-74.
[9]Chen J,Wang W H,Wu F H,et al.Hydrogen sulfide alleviates aluminum toxicity in barley seedlings[J].Plant and Soil,2013,362(1/2):301-318.
[10]Chen X,Wang J,Shi Y,et al.Effects of cadmium on growth and photosynthetic activities in pakchoi and mustard[J].Botanical Studies,2011,52(1):41-46.
[11]Dean C,Pichersky E,Dunsmuir P.Structure,evolution,and regulation of RbcS genes in higher plants[J].Annual Review of Plant Physiology and Plant Molecular Biology,1989,40(1):415-439.
[12]Sclmeider G,Lindqvist Y,Branden C I.Rubisco:Structure and mechanism[J].Annual Review of Biophysics and Biomolecular Structure,1992,21:119-143.
[13]Spreitzer R J.Role of the small subunit in ribulose-1,5-bisphosphate carboxylase/oxygenase[J].Archives of Biochemistry and Biophysics,2003,414(2):141-149.
[14]王维光,李立人.菠菜二磷酸核酮糖(RuBP)羧化酶简化提纯研究[J].植物生理学报,1980(3):257-262.WANG Wei-guang,LI Li-ren.Simplified purification of spinosa diphosphate ketone(RuBP)carboxylase[J].Plant Physiology Journal,1980(3):257-262.
[15]金竹萍,裴雁曦.植物H2S气体信号分子的生理功能研究进展[J].中国细胞生物学学报,2013,35(6):880-888.JIN Zhu-ping,PEI Yan-xi.Research progress on hydrogen sulfide signaling in plants[J].Chinese Journal of Cell Biology,2013,35(6):880-888.
[16]Chen J,Wu F H,Wang W H,et al.Hydrogen sulphide enhances photosynthesis through promoting chloroplast biogenesis,photosynthetic enzyme expression,and thiol redox modification in Spinacia oleracea seedlings[J].Journal of Experimental Botany,2011,62(13):4481-4493.
[17]冯世静,杨途熙,张艳军,等.镉胁迫对杨树光合特性的影响[J].农业环境科学学报,2013,32(3):539-547.FENG Shi-jing,YANG Tu-xi,ZHANG Yan-jun,et al.Effects of cadmium on photosynthetic gas exchange and chlorophyll fluorescence of two species of Poplar[J].Journal of Agro-Environment Science,2013,32(3):539-547.
[18]王鸿蕉,张丽萍,刘志强,等.外源硫化氢对冷胁迫下白菜幼苗生长和光合作用的影响[J].西北植物学报,2015,35(4):780-786.WANG Hong-jiao,ZHANG Li-ping,LIU Zhi-qiang,et al.Influence of H2S on growth and photosynthesis of Brassica rapa var.pekinensis under chilling stress[J].Acta Botanica Boreali-Occidentalia Sinica,10163852015,35(4):780-786.
[19]孙丽敏,金竹萍,钱嘉航,等.干旱胁迫下H2S信号增强植物叶片的光合作用[J].中国细胞生物学学报,2017,35(12):1583-1591.SUN Li-min,JIN Zhu-ping,QIAN Jia-hang,et al.H2S enhanced photosynthesis in response to drought stress[J].Chinese Journal of Cell Biology,2017,35(12):1583-1591.
[20]Krause G H,Weis E.Chlorophyll fluorescence and photosynthesis:The basics[J].Annual Review of Plant Physiology and Plant Molecular Biology,1991,42(1):313-349.
[21]裴雁曦,贺烽,解梦洁,等.气体信号分子H2S和NO对冷胁迫下大白菜幼苗光合作用的影响[J].山西大学学报(自然科学版),2017,40(3):596-601.PEI Yan-xi,HE Feng,XIE Meng-jie,et al.Effects of gasotransmitters NO and H2S on photosynthesis of Chinese cabbage seedlings under chilling stress[J].Journal of Shanxi University(Natural Science Edition),2017,40(3):596-601.
[22]Denise S,Lorenzo L,Carlos G,et al.Gasotransmitters and stomatal closure:Is there redundancy,concerted action,or both?[J].Frontiers in Plant Science,2016,7:1-5.
[23]Qiao Z J,Jing T,Liu Z Q,et al.H2S acting as a downstream signaling molecule of SA regulates Cd tolerance in Arabidopsis[J].Plant and Soil,2015,393:137-146.
[24]Mustafa A K,Gadalla M M,Sen N,et al.H2S signals through protein S-sulfhydration[J].Science Signaling,2009,2(96):ra72.