猪圆环病毒与胸膜肺炎放线杆菌混合感染的诊治
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摘要
2019年3月,大庆某规模化猪场个别猪出现发育不良、咳喘、消瘦、皮肤苍白和呼吸困难等症状,随后出现死亡。为明确发病原因,降低损失,对送检病死猪进行临床症状观察、剖检及实验室检测,并对病原菌进行药物敏感性试验。检测结果为致病性胸膜肺炎放线杆菌和猪圆环病毒混合感染所致,分离的胸膜肺炎放线杆菌对阿莫西林和头孢唑高度敏感。本研究为养殖场饲养猪的死亡病因分析和防治提供了参考。
In March 2019, some pigs in a large-scale pig farm in Daqing showed signs of dysplasia, cough and wheezing, emaciation, pale skin and dyspnea, and then died. In order to clarify the cause of the disease and reduce the loss, the clinical symptoms, autopsy and laboratory tests were carried out on the dead pigs, and the drug sensitivity test was carried out on the pathogenic bacteria. The results were caused by the mixed infection of Actinobacillus pleuropneumoniae and Porcine circovirus. The isolated Actinobacillus pleuropneumoniae was highly sensitive to amoxicillin and cefazol. This study provides a reference for the cause analysis and prevention of pig death in this farm.
In March 2019, some pigs in a large-scale pig farm in Daqing showed signs of dysplasia, cough and wheezing, emaciation, pale skin and dyspnea, and then died. In order to clarify the cause of the disease and reduce the loss, the clinical symptoms, autopsy and laboratory tests were carried out on the dead pigs, and the drug sensitivity test was carried out on the pathogenic bacteria. The results were caused by the mixed infection of Actinobacillus pleuropneumoniae and Porcine circovirus. The isolated Actinobacillus pleuropneumoniae was highly sensitive to amoxicillin and cefazol. This study provides a reference for the cause analysis and prevention of pig death in this farm.
引文
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[2]Jiang Y,et al.Sim ultaneous detection of porcine circovirus type 2,classical swine fever virus,porcine parvovirus and porcine re productive and respiratory syndrom e virus in pigs by m ultiplex polym erase chain reac‐tion[J].The Vet erinary Journal,2010,183(2):172-175.
[3]迟兰,李心海,王兵.徐州地区猪圆环病毒病的流行病学调查[J].黑龙江畜牧兽医,2017(6):143-146.
[4]赵军,周桐枫,刘鹏娟,等.四川地区腹泻仔猪群猪圆环病毒Ⅱ型分子流行病学调查[J].中国预防兽医学报,2016,38(7):546-549.
[5]焦文强,王治方,王克领,等.河南省及周边地区猪圆环病毒2型分子流行病学调查[J].河南农业科学,2017,46(5):125-129.
[6]李瑞香.规模化猪场猪圆环病毒2型的血清流行病学调查[J].动物医学进展,2017,38(3):129-132.
[7]HAESEBO UEK F,CHIE S K,OVEB EKE V I,et al.Actinobacillus Pleuropneumoniae infections in pigs:the role of virulence factors in patho‐genesis and protection[J].Vet Microbiol,1997,58(2/3/4):239-249.
[8]张飞,郑培,曹剑,等.猪胸膜肺炎放线杆菌的分离鉴定及致病性研究[J].黑龙江畜牧兽医,2016(2):151-154.
[9]薛云,赵战勤,陈杨,等.多重PCR方法快速检测猪的5种呼吸道病菌[J].畜牧兽医学报,2009,40(8):1222-1228.
[10]王永祥.猪圆环病毒2型山东株的分离鉴定及其ORF2基因的克隆与表达[D].泰安:山东农业大学,2008.
[11]Parashar U D,Gibson C J,Bresee J S,et al.Rotavirus and severe childhood diarrhea[J].Emerging Infectious Diseases,2006,12(2):304.
[12]季峥嵘.鸭源致病性大肠杆菌的分离与药敏试验[J].中国动物保健,2014(5):19-22.
[13]中国农业科学院哈尔滨兽医研究所.动物传染病学[M].北京:中国农业出版社,2008.
[14]陆承平.兽医微生物学(第四版)[M].北京:中国农业出版社,2007.
[15]谭珊,宋欣媛,吴培福.鸡源志贺氏菌的分离鉴定及耐药性研究[J].现代畜牧兽医,2019(3):12-16.
[16]洪英,黄雁,魏良宇.浅谈纸片扩散法药敏试验[J].福建畜牧兽医,2004,26(5):56.