一株拮抗禾谷镰刀菌和降解呕吐毒素解淀粉芽孢杆菌的筛选及在饲料贮存中的应用
|
摘要
禾谷镰刀菌是引起农作物赤霉病的主要病原菌之一,其次生代谢产物呕吐毒素对人和动物有极大的安全危害,呕吐毒素一旦污染饲料不仅会造成巨额的经济损失,更有可能进入食物链威胁到人与动物的健康安全。目前我国鲜有高效的脱毒方法来应对呕吐毒素对于饲料的安全威胁。为解决上述问题,从江苏省淮安市土壤中筛选出1株同时具有禾谷镰刀菌抗性与呕吐毒素降解能力的解淀粉芽孢杆菌。解淀粉芽孢杆菌本身就是优异的饲料益生菌种,用高效液相色谱和质谱联用的方法分析该菌株后发现其含有杆菌霉素L和泛革素2种脂肽类抗生素,对禾谷镰刀菌的抑菌率达60%;经液相色谱检测后表明该菌在14 d后呕吐毒素转化率达到92.440%。数据表明该菌在饲料防霉与脱毒方面表现出良好的应用前景。
引文
[1]方兴洲.小麦赤霉病研究进展[J].现代农业科技,2014(23):134-135.
[2]Cui L,Selvaraj J N,Xing F G,et al.A minor survey of deoxynivalenol in Fusarium infected wheat from Yangtze-Huaihe river basin region in China[J].Food Control,2013,30(2):469-473.
[3]Pestka J J.Deoxynivalenol:mechanisms of action,human exposure,and toxicological relevance[J].Archives of Toxicology,2010,84(9):663-679.
[4]Maresca M,Mahfoud R,Garmy N,et al.The mycotoxin deoxynivalenol affects nutrient absorption in human intestinal epithelial cells[J].The Journal of Nutrition,2002,132(9):2723-2731.
[5]Koch P.State of the art of trichothecenes analysis[J].Toxicology Letters,2004,153(1):109-112.
[6]Morooka N,Uratsuji N,Yoshizawa T,et al.Studies on the toxic substances in barley in fected with Fusarium spp.[J].Journal of the Food Hygienic Society of Japan,1972,13(5):368-375.
[7]Yoshizawa T,Morooka N.Biological modification of trichothecene mycotoxins:acetylation and deacetylation of deoxynivalenols by Fusarium spp.[J].Applied Microbiology,1975,29(1):54-58.
[8]Moss M O,Thrane U.Fusarium taxonomy with relation to trichothecene formation[J].Toxicology Letters,2004,153(1):23-28.
[9]Thuvander A,Wikman C,Gadhasson I.In vitro exposure of human lymphocytes to trichothecenes:individual variation in sensitivity and effects of combined exposure on lymphocyte function[J].Food and Chemical Toxicology,1999,37(6):639-648.
[10]吴永宁.现代食品安全科学[M].北京:化学工业出版社,2003:311.
[11]Moe N K T,Su M T,Suzuki K,et al.Production of an antibacterial substance by Bacillus mojavensis strain F412 isolated from a Myanmar shrimp product fermented with boiled rice[J].Fisheries Science,2015,81(4):795-802.
[12]陈哲,黄静,赵佳,等.解淀粉芽孢杆菌抑菌机制的研究进展[J].生物技术通报,2015,31(6):37-41.
[13]Skadsen R W,Hohn T M.Use of Fusarium graminearum transformed with gfp to follow infection patterns in barley and Arabidopsis[J].Physiological and Molecular Plant Pathology,2004,64(1):45-53.
[14]Priest F G,Goodfellow M,Shute L A,et al.Bacillus amyloliquefaciens sp.nov.,norn.rev.[J].International Journal of Systematic Bacteriology,1987,37(1):69-71.
[15]Xu Z H,Shao J H,Li B,et al.Contribution of bacillmoycin D in Bacillus amyloliquefaciens SQR9 to antifungal activity and biofilm formation[J].Applied and Environmental Microbiology,2013,79(3):808-815.
[16]Sutyak K E,Wirawan R E,Aroutcheva A A,et al.Isolation of the Bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived Bacillus amyloliquefaciens[J].Journal of Applied Microbiology,2008,104(4):1067-1074.
[17]计坚.解淀粉芽孢杆菌SC06介导猪肠道免疫机理的研究[D].杭州:浙江大学,2011.
[18]沈勇涛.芽孢杆菌对母猪仔猪微生态的影响及其生长条件优化[D].武汉:华中农业大学,2011.
[19]Chen Y,Li J,Xiao P,et al.Isolation and characterization of Bacillus spp.M001 for potential application in turbot(Scophthalmus maximus L.)against Vibrio anguillarum[J].Aquaculture Nutrition,2016,22(2):374-381.
[20]权春善,王军华,徐洪涛,等.一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究[J].微生物学报,2006,46(1):7-12.
[21]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.卓国荣,周红蕾,卢炜,等.泰州地区宠物弓形虫感染流行现状调查与防治[J].江苏农业科学,2019,47(8):191-194.
[2]Cui L,Selvaraj J N,Xing F G,et al.A minor survey of deoxynivalenol in Fusarium infected wheat from Yangtze-Huaihe river basin region in China[J].Food Control,2013,30(2):469-473.
[3]Pestka J J.Deoxynivalenol:mechanisms of action,human exposure,and toxicological relevance[J].Archives of Toxicology,2010,84(9):663-679.
[4]Maresca M,Mahfoud R,Garmy N,et al.The mycotoxin deoxynivalenol affects nutrient absorption in human intestinal epithelial cells[J].The Journal of Nutrition,2002,132(9):2723-2731.
[5]Koch P.State of the art of trichothecenes analysis[J].Toxicology Letters,2004,153(1):109-112.
[6]Morooka N,Uratsuji N,Yoshizawa T,et al.Studies on the toxic substances in barley in fected with Fusarium spp.[J].Journal of the Food Hygienic Society of Japan,1972,13(5):368-375.
[7]Yoshizawa T,Morooka N.Biological modification of trichothecene mycotoxins:acetylation and deacetylation of deoxynivalenols by Fusarium spp.[J].Applied Microbiology,1975,29(1):54-58.
[8]Moss M O,Thrane U.Fusarium taxonomy with relation to trichothecene formation[J].Toxicology Letters,2004,153(1):23-28.
[9]Thuvander A,Wikman C,Gadhasson I.In vitro exposure of human lymphocytes to trichothecenes:individual variation in sensitivity and effects of combined exposure on lymphocyte function[J].Food and Chemical Toxicology,1999,37(6):639-648.
[10]吴永宁.现代食品安全科学[M].北京:化学工业出版社,2003:311.
[11]Moe N K T,Su M T,Suzuki K,et al.Production of an antibacterial substance by Bacillus mojavensis strain F412 isolated from a Myanmar shrimp product fermented with boiled rice[J].Fisheries Science,2015,81(4):795-802.
[12]陈哲,黄静,赵佳,等.解淀粉芽孢杆菌抑菌机制的研究进展[J].生物技术通报,2015,31(6):37-41.
[13]Skadsen R W,Hohn T M.Use of Fusarium graminearum transformed with gfp to follow infection patterns in barley and Arabidopsis[J].Physiological and Molecular Plant Pathology,2004,64(1):45-53.
[14]Priest F G,Goodfellow M,Shute L A,et al.Bacillus amyloliquefaciens sp.nov.,norn.rev.[J].International Journal of Systematic Bacteriology,1987,37(1):69-71.
[15]Xu Z H,Shao J H,Li B,et al.Contribution of bacillmoycin D in Bacillus amyloliquefaciens SQR9 to antifungal activity and biofilm formation[J].Applied and Environmental Microbiology,2013,79(3):808-815.
[16]Sutyak K E,Wirawan R E,Aroutcheva A A,et al.Isolation of the Bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived Bacillus amyloliquefaciens[J].Journal of Applied Microbiology,2008,104(4):1067-1074.
[17]计坚.解淀粉芽孢杆菌SC06介导猪肠道免疫机理的研究[D].杭州:浙江大学,2011.
[18]沈勇涛.芽孢杆菌对母猪仔猪微生态的影响及其生长条件优化[D].武汉:华中农业大学,2011.
[19]Chen Y,Li J,Xiao P,et al.Isolation and characterization of Bacillus spp.M001 for potential application in turbot(Scophthalmus maximus L.)against Vibrio anguillarum[J].Aquaculture Nutrition,2016,22(2):374-381.
[20]权春善,王军华,徐洪涛,等.一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究[J].微生物学报,2006,46(1):7-12.
[21]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.卓国荣,周红蕾,卢炜,等.泰州地区宠物弓形虫感染流行现状调查与防治[J].江苏农业科学,2019,47(8):191-194.