基于SSR标记的68份红毛丹种质资源DNA指纹图谱构建
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摘要
本研究利用筛选出的10对多态性高、条带清晰的SSR引物,以海南岛68份红毛丹种质为材料,进行遗传多样性分析和SSR指纹图谱的构建。结果表明:10对引物在供试材料中共检测出20个等位位点,多态性条带比例为100%,平均多态性信息含量(PIC)为0.393;对68份红毛丹种质做聚类分析,遗传相似系数为0.290～0.664;采用引物?带型组合法构建了68份红毛丹种质的指纹图谱。该结果为今后红毛丹种质鉴定和分子育种提供重要依据。
The SSR fingerprint map and genetic diversity of 68 rambutan(Nephelium lappaceum L.) accessions were studied using 10 pairs of selected polymorphic SSR primers.The results indicated that all the primers showed polymorphism,and 20 polymorphic alleles were revealed and the average polymorphic information content(PIC) was 0.393.The genetic similarities among accessions ranged from 0.290 to 0.664,on which a phylogenetic tree showing the relationship among the accessions was constructed.A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 68 cultivars.The DNA fingerprinting provides a technical reference for the varieties identification and molecular breeding in rambutan.
The SSR fingerprint map and genetic diversity of 68 rambutan(Nephelium lappaceum L.) accessions were studied using 10 pairs of selected polymorphic SSR primers.The results indicated that all the primers showed polymorphism,and 20 polymorphic alleles were revealed and the average polymorphic information content(PIC) was 0.393.The genetic similarities among accessions ranged from 0.290 to 0.664,on which a phylogenetic tree showing the relationship among the accessions was constructed.A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 68 cultivars.The DNA fingerprinting provides a technical reference for the varieties identification and molecular breeding in rambutan.
引文
[1]Chew P C,Clyde M M,Normah M N,et al.Genetic diversity and relatedness among accessions of rambutan(Nephelium Lappaceum L.)[J].Malaysian Applied Biology Journal,2005,34(1):21-29.
[2]Anggraheni Y G D,Mulyaningsih E S.Evaluation of nine Rambutan(Nephelium lappaceum)varieties genetic diversity using RAPD markers[J].Biopropal Industri,2018,9(1):1-8.
[3]Andrade RAD,Wickert E,Martins ABG,et al.Genetic diversity among stock plants and progenies of rambutan[J].Revista Brasileira de Fruticultura,2012,34(2):630-634.
[4]Christyne S N,Tatik C,Nina R D.Genetic diversity of wild rambutans(Nephelium spp.)in Sanggau,West Kalimantan based on SSR and ISSR markers[J].Floribunda,2016,5(4),115-125.
[5]林兴娥,葛宇,周兆禧,等.一种高质量的红毛丹基因组DNA提取方法[J].热带农业科学,2015,35(8):16-20.
[6]范建光,张海英,宫国义,等.西瓜DUS测试标准品种SSR指纹图谱构建及应用[J].植物遗传资源学报,2013,14(5):892-899.
[7]宋海斌,崔喜波,马鸿艳,等.基于SSR标记的甜瓜品种(系)DNA指纹图谱库的构建[J].中国农业科学,2012,45(13):2676-2689.
[8]Kuswandi K,Sobir S,Suwarno W B.Genetic variation of rambutan germplasmin indonesia based on morphological characters[J].Horticulture Journal,2014,24(4):289-298.
[9]许鲲,李锋,吴金锋,等.SSR荧光标记毛细管电泳法与国家冬油菜区试指纹鉴定平台的构建[J].中国油料作物学报,2014,36(2):150-159.
[10]任小平,郑艳丽,黄莉,等.花生SSR核心引物筛选及育成品种DNA指纹图谱构建[J].中国油料作物学报,2016,38(5):563-571.
[2]Anggraheni Y G D,Mulyaningsih E S.Evaluation of nine Rambutan(Nephelium lappaceum)varieties genetic diversity using RAPD markers[J].Biopropal Industri,2018,9(1):1-8.
[3]Andrade RAD,Wickert E,Martins ABG,et al.Genetic diversity among stock plants and progenies of rambutan[J].Revista Brasileira de Fruticultura,2012,34(2):630-634.
[4]Christyne S N,Tatik C,Nina R D.Genetic diversity of wild rambutans(Nephelium spp.)in Sanggau,West Kalimantan based on SSR and ISSR markers[J].Floribunda,2016,5(4),115-125.
[5]林兴娥,葛宇,周兆禧,等.一种高质量的红毛丹基因组DNA提取方法[J].热带农业科学,2015,35(8):16-20.
[6]范建光,张海英,宫国义,等.西瓜DUS测试标准品种SSR指纹图谱构建及应用[J].植物遗传资源学报,2013,14(5):892-899.
[7]宋海斌,崔喜波,马鸿艳,等.基于SSR标记的甜瓜品种(系)DNA指纹图谱库的构建[J].中国农业科学,2012,45(13):2676-2689.
[8]Kuswandi K,Sobir S,Suwarno W B.Genetic variation of rambutan germplasmin indonesia based on morphological characters[J].Horticulture Journal,2014,24(4):289-298.
[9]许鲲,李锋,吴金锋,等.SSR荧光标记毛细管电泳法与国家冬油菜区试指纹鉴定平台的构建[J].中国油料作物学报,2014,36(2):150-159.
[10]任小平,郑艳丽,黄莉,等.花生SSR核心引物筛选及育成品种DNA指纹图谱构建[J].中国油料作物学报,2016,38(5):563-571.