四倍体野菊与露地菊‘紫秋裳’种间杂交 F_1代3个株系倍性鉴定
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摘要
采用传统根尖压片技术对母本四倍体野菊(Dendrathema indicum)与父本露地菊‘紫秋裳’(Dendrathema×gandiflorum‘ziqiushang’)种间杂交F_1代3个株系(‘S05’、‘S09’、‘S10’)进行染色体计数观察,并运用流式细胞仪测定样品的荧光强度值进而鉴定其倍性。结果表明:母本四倍体野菊、父本露地菊‘紫秋裳’、‘S05’、‘S09’、‘S10’的染色体数目分别为36条、52～54条、36条、35～38条、36～38条,该试验的染色体计数结果不同于理论上由遗传规律得到的结果;利用流式细胞仪检测荧光强度峰值分别为344.98、401.36、327.64、371.75、309.41,但检测结果受外界影响,准确性有待进一步探讨。
The chromosome counts of three F_1 strains(‘S05', ‘S09' and ‘S10') of interspecific hybridization between female tetraploid(Dendrathemaindicum) and the father of the genus(Dendrathema×gandiflorum ‘ziqiushang') were observed by using traditional root tip tablet technology, and the fluorescence intensity value of the samples was determined by flow cytometry to identify the ploidy. The number of chromosomes of the female tetraploid(Dendrathemaindicum), the father of the genus(Dendrathema×gandiflorum ‘ziqiushang'), ‘S05', ‘S09' and ‘S10' were 36, 52-54, 36, 35-38 and 36-38, respectively, and the results of chromosome count were different from those obtained theoretically by genetic law.The peak fluorescence intensity detected by flow cytometry were 344.98, 401.36, 327.64, 371.75, and 309.41, respectively, but the test results were affected by external influences, and the accuracy needed to be further explored.
The chromosome counts of three F_1 strains(‘S05', ‘S09' and ‘S10') of interspecific hybridization between female tetraploid(Dendrathemaindicum) and the father of the genus(Dendrathema×gandiflorum ‘ziqiushang') were observed by using traditional root tip tablet technology, and the fluorescence intensity value of the samples was determined by flow cytometry to identify the ploidy. The number of chromosomes of the female tetraploid(Dendrathemaindicum), the father of the genus(Dendrathema×gandiflorum ‘ziqiushang'), ‘S05', ‘S09' and ‘S10' were 36, 52-54, 36, 35-38 and 36-38, respectively, and the results of chromosome count were different from those obtained theoretically by genetic law.The peak fluorescence intensity detected by flow cytometry were 344.98, 401.36, 327.64, 371.75, and 309.41, respectively, but the test results were affected by external influences, and the accuracy needed to be further explored.
引文
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[2] BROCHMANN C,BRYSTING A K,ALSOS I G.Polyploidy in arctic plants[J].Biol J Linn Soc,2004,82(4):521-536.
[3] 李懋学,张斅方,陈俊愉.我国某些野生和栽培菊花的细胞学研究[J].园艺学报,1983,10(3):199-206,219-222.
[4] BAO W K,YAN Y R.Octoploid triticale in China.advance in science of China[J].Biology,1993(3):55-76.
[5] 王荣邦,张秀海,吴忠义,等.菊花染色体倍性鉴定研究[J].安徽农业科学,2010,38(23):12778-12780,12789.
[6] 朱明丽,刘倩倩,戴思兰.38个中国大菊品种染色体核型参数的统计分析[J].植物学报,2011,46(4):447-455.
[7] 高文杰,周蕴薇,何淼.四倍体神农香菊与露地菊‘纽9717’种间杂交初步研究[J].湖南农业大学学报(自然科学版),2016,42(4):374-379.
[8] 何淼,董春艳,冯博,等.神农香菊组织培养和植株再生[J].东北林业大学学报,2010,38(7):64-66.
[9] KIM J S,PAK J H,SEO B B.Karyotypes of metaphase chromosomes in diploid populations of Dendranthema zawadskii and related species (Asteraceae) from Korea:diversity and evolutionary implications[J].Journal of Plant Research,2003,116(1):47-55.
[10] 陈发棣,陈秀兰,房伟民.3个地理居群野菊的细胞学研究[R].南京:中国园艺学会,1998.
[11] 丁玲,陈发棣,房伟民.菊属8个种27份材料遗传多样性的同工酶分析[J].西北植物学报,2007,27(2):249-256.
[12] 田路明,齐丹,曹玉芬,等.利用流式细胞仪鉴定梨种质资源染色体倍性[J].中国果树,2018(3):29-32.
[13] 黄少玲.春石斛兰品种的倍性鉴定及RAPD分子遗传图谱的构建[D].武汉:华中农业大学,2007.
[14] 李明银,何云晓.植物遗传嵌合体及其在观赏植物育种中的应用[J].植物学通报,2005,22(6):641-647.