核、质基因对肺形侧耳菌丝生长、营养成分及基因表达的影响
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摘要
本研究通过杂交构建肺形侧耳同核异质菌株N1M1、N1M2和同质异核菌株N1M1、N2M1,比较菌丝形态与生长速度、营养成分以及常见的细胞核基因与线粒体基因的表达量,分析线粒体基因对肺形侧耳菌丝的影响,探讨线粒体基因与核基因的相互作用。由菌丝生长情况可知N1M1和N1M2菌丝形态相似,生长速度差异不显著,N1M1和N2M1菌丝形态差异大,生长速度差异极显著,在菌丝形态与生长速度上细胞核基因作用大于线粒体基因。进一步检测菌株中的主要营养成分发现必需氨基酸与总水解氨基酸含量差异显著,菌株N1M2蛋白含量显著高于N1M1,N1M1维生素C含量是N1M2的1.67倍,菌株N2M1多糖和蛋白含量显著高于N1M1,铁和维生素C含量显著低于N1M1。所以细胞核基因、线粒体基因都能影响肺形侧耳营养成分含量。检测同核异质菌株N1M1、N1M2的7个细胞核常见基因的表达情况发现,N1M2菌丝中6个细胞核基因的表达量都显著高于N1M1,这表明肺形侧耳线粒体基因的不同会影响核基因的表达;同质异核菌株N1M1、N2M1的14个线粒体普通编码蛋白基因表达差异显著,这说明线粒体基因的表达量会因核基因的不同有所差异。综上,肺形侧耳线粒体基因和细胞核基因能够相互影响,共同作用于生命活动。
Isonuclear alloplasmic strains N1 M1 and N1 M2 and isocytoplasmic allonuclear strains N1 M1 and N2 M1 of Pleurotus pulmonarius were obtained by hybridization technique. In order to analyze the effects of mitochondrial genes on mycelium and search into the interaction between mitochondrial genes and nuclear genes, mycelium growth rate, nutrient composition and the expression of several common nuclear and mitochondrial genes of N1 M1, N1 M2 and N2 M1 were compared. Observation of mycelium growth showed that N1 M1 and N1 M2 had similar mycelial morphology and no significant difference in growth rate, however mycelial morphology and growth rate of strains N1 M1 and N2 M1 had significant difference, indicating that nuclear genes played a greater role than mitochondrial genes in mycelial morphology and growth rate. Further determination of the main nutrient components showed that the content of essential amino acids and total hydrolyzed amino acids in different strains was significantly different. The protein content of strain N1 M2 was significantly higher than that of strain N1 M1. The vitamin C content of strain N1 M1 was 1.67 times higher than that of strain N1 M2. The content of polysaccharide and protein of strain N2 M1 was significantly higher than that of strain N1 M1, while the content of iron and vitamin C was significantly lower than that of strain N1 M1.Facts had proved that both nuclear genes and mitochondrial genes could affect the nutrient content of Pleurotus pulmonarius. The expression of seven common genes in nuclei of isonuclear alloplasmic strains N1 M1 and N1 M2 was detected. As a result, six of the genes in N1 M2 was significantly higher in genes expression as compared with those in N1 M1. It indicated that the different mitochondrial genes of Pleurotus pulmonarius could affect the expression of nuclear genes. There were significant differences in the expression of 14 common mitochondrial coding protein genes in isocytoplasmic allonuclear strains N1 M1 and N2 M1,indicating that the expression of mitochondrial genes significantly differs in accordance with different nuclear genes. In conclusion, mitochondrial and nuclear genes of Pleurotus pulmonarius can interact each other and act on life activities jointly.
Isonuclear alloplasmic strains N1 M1 and N1 M2 and isocytoplasmic allonuclear strains N1 M1 and N2 M1 of Pleurotus pulmonarius were obtained by hybridization technique. In order to analyze the effects of mitochondrial genes on mycelium and search into the interaction between mitochondrial genes and nuclear genes, mycelium growth rate, nutrient composition and the expression of several common nuclear and mitochondrial genes of N1 M1, N1 M2 and N2 M1 were compared. Observation of mycelium growth showed that N1 M1 and N1 M2 had similar mycelial morphology and no significant difference in growth rate, however mycelial morphology and growth rate of strains N1 M1 and N2 M1 had significant difference, indicating that nuclear genes played a greater role than mitochondrial genes in mycelial morphology and growth rate. Further determination of the main nutrient components showed that the content of essential amino acids and total hydrolyzed amino acids in different strains was significantly different. The protein content of strain N1 M2 was significantly higher than that of strain N1 M1. The vitamin C content of strain N1 M1 was 1.67 times higher than that of strain N1 M2. The content of polysaccharide and protein of strain N2 M1 was significantly higher than that of strain N1 M1, while the content of iron and vitamin C was significantly lower than that of strain N1 M1.Facts had proved that both nuclear genes and mitochondrial genes could affect the nutrient content of Pleurotus pulmonarius. The expression of seven common genes in nuclei of isonuclear alloplasmic strains N1 M1 and N1 M2 was detected. As a result, six of the genes in N1 M2 was significantly higher in genes expression as compared with those in N1 M1. It indicated that the different mitochondrial genes of Pleurotus pulmonarius could affect the expression of nuclear genes. There were significant differences in the expression of 14 common mitochondrial coding protein genes in isocytoplasmic allonuclear strains N1 M1 and N2 M1,indicating that the expression of mitochondrial genes significantly differs in accordance with different nuclear genes. In conclusion, mitochondrial and nuclear genes of Pleurotus pulmonarius can interact each other and act on life activities jointly.
引文
Cao QC,Zhang YF,Hu P,Cui HF,Yu XP,Ye ZH,2017.Cloning and expression analysis of hyphal formation-related gene UeSsk2 in Ustilago esculenta.Journal of China University of Metrology,28(2):252-260(in Chinese)
Chen JC,Shen HS,Tang BS,Yang J,Liu T,Yang JJ,2003.Study on technique for efficient cultivation of a edible fungus[Pleurotus cornucopiae(Paul.:Pers.)Rolland]out of season.Edible Fungi of China,22(4):21-23(in Chinese)
Chen LF,Liu JZ,2005.The coordinated regulation between nuclear DNA and mitochondria DNA.Chinese Journal of Pathophysiology,21(8):1614-1618(in Chinese)
Chen GG,Lin Y,Liu XR,Zhao GH,Chen J,2017.Recent advances in genetics and breeding of Pleurotus pulmonarius.Chinese Journal of Tropical Crops,38(7):1377-1381(in Chinese)
Cui YZ,Tao L,Sun CH,Liu P,Li GH,Qin QM,2016.The ABCtransporter,BcAtm1,is involved in conidial germination and pathogenicity of Botrytis cinerea.Acta Phytopathologica Sinica,46(5):614-623(in Chinese)
Dai YC,Yang ZL,2008.A revised checklist of medicinal fungi in China.Mycosystema,27:801-824
Desmond E,Brochier-Armanet C,Forterre P,Gribaldo S,2011.On the last common ancestor and early evolution of eukaryotes:reconstructing the history of mitochondrial ribosomes.Research in Microbiology,162(1):53-70
Gao CP,Zhong LF,Ren X,Jiang LP,Geng CY,Yao XF,Cao J,2011.Effects of mitochondrial DNA-depletion and functional deficiency on expression of nuclear DNA.China Public Health,27(1):50-51(in Chinese)
Garesse R,Vallejo CG,2001.Animal mitochondrial biogenesis and function:a regulatory cross-talk between two genomes.Gene,263(1):1-16
Graziewicz MA,Day BJ,Copeland WC,2002.The mitochondrial DNA polymerase as a target of oxidative damage.Nucleic Acids Research,30(13):2817-2824
Gu ML,Wang YJ,Chen S,Zhang YB,2009.Mechanisms of coordination of gene expression between mitochondrial and nuclear genomes.Chemistry of Life,29(6):803-811(in Chinese)
Jiang ZH,Xiao SX,Lei JG,Wang YX,Wen BQ,2009.Nutritional value of protein of Panus giganteus’s fruit bodies under the condition of exogenous addition of Zn.Journal of Agro-Environment Science,28(3):449-453(in Chinese)
Kuang D,2011.Analysis and comparison of nutritional components of seven edible fungi.Edible Fungi,4:57-59(in Chinese)
Li HM,Wang YY,Gao T,Xu CF,Shi ZQ,Chen J,Xu XF,2018.FgPDK1 modulates osmotic stress responses in Fusarium graminearum.Journal of Nanjing Normal University(Natural Science Edition),41(1):76-82(in Chinese)
Li QZ,Wu D,Chen MJ,Li Y,Song XX,Zhou F,Li ZP,Yang Y,2015.Nutritional assessment of protein from H.erinaceus fruit bodies harvested at different developmental stages.Farm Produces Processing,12:53-56(in Chinese)
Li WH,Wu X,Gao XX,Feng LY,Jia YP,Cheng XH,2018.Comparative analysis of twelve Pleurotus pulmonarius strains.Northern Horticulture,5:145-149(in Chinese)
Ling F,Hori A,Shibata T,2007.DNA recombination-initiation plays a role in the extremely biased inheritance of yeast[rho-]mitochondrial DNA that contains the replication origin ori5.Molecular&Cellular Biology,27(3):1133-45
Liu M,Xiao ZT,Qiu YM,Qiu GG,He HQ,2016.Utilization of industry-cultivated Flammulina velutipes spent compost as substrate for Pleurotus geesteranus cultivation.Guangdong Agricultural Sciences,43(10):48-52(in Chinese)
Lu X,Zheng QX,Ye LY,Ding ZQ,Chu YR,Huang QX,Wu XP,2017.Identification and biological characteristics analysis of a wild Pleurotus pulmonarius.Genomics and Applied Biology,36(5):1997-2004(in Chinese)
Lu ZH,2008.SRAP analysis of cultivation strains of Pleurotus pulmonarius and its sibling varieties.Acta Agriculturae Jiangxi,20(11):8-10(in Chinese)
Ma HY,Bai BL,2014.The cultural techniques of Pleurotus geesteranus.Northern Horticulture,13:140-141(in Chinese)
Nguyen TK,Im KH,Choi J,Shin PG,Lee TS,2016.Evaluation of antioxidant,anti-cholinesterase,and anti-inflammatory effects of culinary mushroom Pleurotus pulmonarius.Mycobiology,44(4):291-301
Schaefer L,Engman H,Miller JB,2000.Coding sequence,chromosomal localization,and expression pattern of Nrf1:the mouse homolog of Drosophila erect wing.Mammalian Genome,11(2):104-110
Shen JW,Wang SM,Qi YC,Gao YQ,Liang ZP,Qiu LY,2007.Comparing the lentinan yield of the monokaryontic mycelia to the dikaryotic mycelia from Lentinula edodes939.Acta Horticulturae Sinica,34(4):941-946(in Chinese)
Su Rong,Shang XD,Xu Z,Tan Q,Chen MJ,Cao H,2009.Bioiogical characteristics of Flammulina velutipes self-bred progenies.Mycosystema,28(3):378-384(in Chinese)
Vanlerberghe GC,Robson CA,Yip JYH,2002.Induction of mitochondrial alternative oxidase in response to a cell signal pathway down-regulating the cytochrome pathway prevents programmed cell death.Plant Physiology,129(4):1829-1842
Wahab NAA,Abdullah N,Aminudin N,2014.Characterisation of potential antidiabetic-related proteins from Pleurotus pulmonarius(Fr.)Quél.(grey oyster mushroom)by MALDI-TOF/TOF mass spectrometry.BioMed Research International,2014(2014):131607-131607
Wang WK,Yuan WD,Fang XP,Song JL,Yan J,Lu N,2017.Comparative proteomics analysis of Pleurotus pulmonarius at different developmental phases.Journal of Zhejiang University(Agriculture&Life Sciences),43(5):527-535(in Chinese)
Weng BQ,Jiang ZH,Lin Y,2002.Effect of different kinds of composts on the nutritional evaluation of protein in Pleurotus geesteranus fruitbodies.Acta Edulis Fungi,9(2):10-13(in Chinese)
Wu SJ,Wu XJ,Chen XF,Wang CQ,Wei SY,2017.Effects of sawdust of Moringa oleifera Lam.on the yield and quality of fruit body of Pleurotus pulmonarius.Chinese Journal of Tropical Crops,38(3):482-486(in Chinese)
Xin Y,Ruan SL,Wang SH,Ma HS,Wang WK,Wang SZ,2008.Cluster analysis of ten Pleurotus geesteranus strains based on random amplification of polymorphic DNA(RAPD)and expressed sequence tag single sequence repeats(ESTSSR)markers.Acta Edulis Fungi,15(4):20-25(in Chinese)
Yang XD,Yang JH,Zhang MF,2011.Possible pathways and mechanisms of mitochondrial retrograde regulation of nuclear gene expression in Brassica juncea.Acta Horticulturae Sinica,38(S):2540(in Chinese)
Ye LY,Meng GL,Cheng B,Zhao LL,Xie F,Wu XP,2018.Mitochondrial genome sequencing and polymorphism analysis of Pleurotus pulmonarius.Mycosystema,37(9):1179-1187(in Chinese)
Ye M,Pan YJ,Ma GR,Chen YX,2001.Genetic analysis of hybrids of the positive and negative di-mon mating in Lentinula edodes.Mycosystema,20(1):94-99(in Chinese)
Zhang JL,Zhang LJ,Li L,Liu JH,Tan Q,Shang XD,2015.Inbreeding and selection of new strain Shenxiu-1 of Pleurotus pulmonarius.Microbiology,42(8):1539-1548(in Chinese)
Zhang QZ,Zhang DQ,Liu QJ,2012.Research status on interaction between nuclear genome and mitochondrial genome in tumorgenesis.Journal of Radiation Research and Radiation Processing,30(2):65-70(in Chinese)
Zhang XY,Zhang B,Xin G,Gong X,2016.Research progress on nutrition constituents,bioactivity and storage preservation of Pleurotus geesteranus.Journal of Food Safety&Quality,6:2314-2319(in Chinese)
Zhou SH,2016.Cloning and expression analysis of the Ppcsl-1gene related to change-temperature fruiting from Pleurotus pulmonarius.Master Thesis,Zhejiang Normal University,Jinhua.37-45(in Chinese)
Zhu J,Liu XR,Xie BG,Liu FY,Deng YJ,Xiong F,2009.Analysis of germplasm resources of Pleurotus geesteranus by ITS-RFLP.Journal of Fujian Agriculture and Forestry University(Natural Science Edition),38(2):186-191(in Chinese)
曹乾超,张雅芬,胡鹏,崔海峰,俞晓平,叶子弘,2017.菰黑粉菌菌丝形成相关基因UeSsk2的克隆及表达分析.中国计量学院学报,28(2):252-260
陈君琛,沈恒胜,汤葆莎,杨菁,刘韬,杨嘉金,2003.秀珍菇反季节高效栽培技术研究.中国食用菌,22(4):21-23
陈丽峰,柳君泽,2005.细胞核基因组与线粒体基因组的相互调控.中国病理生理杂志,21(8):1614-1618
陈躬国,林原,刘新锐,赵光辉,陈剑,2017.秀珍菇遗传育种研究进展.热带作物学报,38(7):1377-1381
崔亚忠,陶岚,孙晨浩,刘平,李桂华,秦庆明,2016.ABC转运蛋白BcAtm1参与灰葡萄孢分生孢子萌发及对寄主植物的侵染.植物病理学报,46(5):614-623
戴玉成,杨祝良,2008.中国药用真菌名录及部分名称的修订.菌物学报,27:801-824
高春鹏,仲来福,任翔,姜丽平,耿成燕,姚晓峰,曹军,2011.线粒体DNA缺失和功能缺失对核基因影响.中国公共卫生,27(1):50-51
顾明亮,汪业军,陈姝,张永彪,2009.线粒体基因与核基因协同表达的机制.生命的化学,29(6):803-811
江枝和,肖淑霞,雷锦桂,王义祥,翁伯琪,2009.添加外源锌对猪肚菇子实体蛋白质营养价值的影响.农业环境科学学报,28(3):449-453
况丹,2011.七种食用菌营养成分分析比较.食用菌,4:57-59
李海梅,王滢莹,高弢,徐寸发,石志琦,陈健,许晓风,2018.FgPDK1调控禾谷镰孢菌响应渗透胁迫研究.南京师大学报(自然科学版),41(1):76-82
李巧珍,吴迪,陈明杰,李玉,宋晓霞,周峰,李正鹏,杨焱,2015.猴头菌子实体不同发育阶段蛋白质营养价值评价.农产品加工,12:53-56
李维焕,吴璇,高兴喜,冯路瑶,贾玉萍,程显好,2018.十二个肺形侧耳菌株的比较试验.北方园艺,5:145-149
刘明,肖自添,邱耀明,邱桂根,何焕清,2016.利用金针菇渣栽培秀珍菇试验.广东农业科学,43(10):48-52
鲁欣,郑秋霞,叶丽云,丁智权,出怡汝,黄清雄,吴小平,2017.一株野生秀珍菇的鉴定及其生物学特性的分析.基因组学与应用生物学,36(5):1997-2004
卢政辉,2008.秀珍菇及其近缘22株菌株的SRAP分析.江西农业学报,20(11):8-10
马洪艳,白宝良,2014.秀珍菇栽培技术.北方园艺,13:140-141
申进文,王淑敏,戚元成,高玉千,梁振普,邱立友,2007.香菇939菌株单核菌丝和双核菌丝多糖产量比较.园艺学报,34(4):941-946
苏蓉,尚晓冬,徐珍,谭琦,陈明杰,曹晖,2009.金针菇自交后代生物学特性的研究.菌物学报,28(3):378-384
王伟科,袁卫东,方献平,宋吉玲,闫静,陆娜,2017.秀珍菇不同生长发育阶段蛋白质组学分析.浙江大学学报(农业与生命科学版),43(5):527-535
翁伯琦,江枝和,林勇,2002.不同培养料对秀珍菇子实体蛋白质营养评价的影响.食用菌学报,9(2):10-13
吴圣进,吴小建,陈雪凤,王灿琴,韦仕岩,2017.辣木屑对秀珍菇子实体产量与品质的影响.热带作物学报,38(3):482-486
忻雅,阮松林,王世恒,马华升,王伟科,王淑珍,2008.基于RAPD和EST-SSR标记的秀珍菇菌株聚类分析.食用菌学报,15(4):20-25
杨晓东,杨景华,张明方,2011.芥菜线粒体反向调控核基因表达的可能途径和机制研究.园艺学报,38(S):2540
叶丽云,孟国良,程冰,赵丽丽,解凡,吴小平,2018.肺形侧耳线粒体基因组多态性鉴定.菌物学报,37(9):1179-1187
叶明,潘迎捷,马桂荣,陈咏萱,2001.香菇正反双单杂交后代的遗传分析.菌物系统,20(1):94-99
张俊玲,章炉军,李亮,刘建辉,谭琦,尚晓冬,2015.自交选育秀珍菇新菌株“申秀1号”.微生物学通报,42(8):1539-1548
张晓玉,张博,辛广,宫雪,2016.秀珍菇营养成分、生物活性及贮藏保鲜的研究进展.食品安全质量检测学报,6:2314-2319
张庆召,张德钦,刘青杰,2012.肿瘤发生中核基因组与线粒体基因组的相互作用.辐射研究与辐射工艺学报,30(2):65-70
周烁红,2016.秀珍菇变温结实相关Ppcsl-1基因的克隆及表达分析.浙江师范大学硕士论文,金华.37-45
朱坚,刘新锐,谢宝贵,刘福阳,邓优锦,熊芳,2009.秀珍菇种质资源的ITS-RFLP分析.福建农林大学学报(自然科学版),38(2):186-191
Chen JC,Shen HS,Tang BS,Yang J,Liu T,Yang JJ,2003.Study on technique for efficient cultivation of a edible fungus[Pleurotus cornucopiae(Paul.:Pers.)Rolland]out of season.Edible Fungi of China,22(4):21-23(in Chinese)
Chen LF,Liu JZ,2005.The coordinated regulation between nuclear DNA and mitochondria DNA.Chinese Journal of Pathophysiology,21(8):1614-1618(in Chinese)
Chen GG,Lin Y,Liu XR,Zhao GH,Chen J,2017.Recent advances in genetics and breeding of Pleurotus pulmonarius.Chinese Journal of Tropical Crops,38(7):1377-1381(in Chinese)
Cui YZ,Tao L,Sun CH,Liu P,Li GH,Qin QM,2016.The ABCtransporter,BcAtm1,is involved in conidial germination and pathogenicity of Botrytis cinerea.Acta Phytopathologica Sinica,46(5):614-623(in Chinese)
Dai YC,Yang ZL,2008.A revised checklist of medicinal fungi in China.Mycosystema,27:801-824
Desmond E,Brochier-Armanet C,Forterre P,Gribaldo S,2011.On the last common ancestor and early evolution of eukaryotes:reconstructing the history of mitochondrial ribosomes.Research in Microbiology,162(1):53-70
Gao CP,Zhong LF,Ren X,Jiang LP,Geng CY,Yao XF,Cao J,2011.Effects of mitochondrial DNA-depletion and functional deficiency on expression of nuclear DNA.China Public Health,27(1):50-51(in Chinese)
Garesse R,Vallejo CG,2001.Animal mitochondrial biogenesis and function:a regulatory cross-talk between two genomes.Gene,263(1):1-16
Graziewicz MA,Day BJ,Copeland WC,2002.The mitochondrial DNA polymerase as a target of oxidative damage.Nucleic Acids Research,30(13):2817-2824
Gu ML,Wang YJ,Chen S,Zhang YB,2009.Mechanisms of coordination of gene expression between mitochondrial and nuclear genomes.Chemistry of Life,29(6):803-811(in Chinese)
Jiang ZH,Xiao SX,Lei JG,Wang YX,Wen BQ,2009.Nutritional value of protein of Panus giganteus’s fruit bodies under the condition of exogenous addition of Zn.Journal of Agro-Environment Science,28(3):449-453(in Chinese)
Kuang D,2011.Analysis and comparison of nutritional components of seven edible fungi.Edible Fungi,4:57-59(in Chinese)
Li HM,Wang YY,Gao T,Xu CF,Shi ZQ,Chen J,Xu XF,2018.FgPDK1 modulates osmotic stress responses in Fusarium graminearum.Journal of Nanjing Normal University(Natural Science Edition),41(1):76-82(in Chinese)
Li QZ,Wu D,Chen MJ,Li Y,Song XX,Zhou F,Li ZP,Yang Y,2015.Nutritional assessment of protein from H.erinaceus fruit bodies harvested at different developmental stages.Farm Produces Processing,12:53-56(in Chinese)
Li WH,Wu X,Gao XX,Feng LY,Jia YP,Cheng XH,2018.Comparative analysis of twelve Pleurotus pulmonarius strains.Northern Horticulture,5:145-149(in Chinese)
Ling F,Hori A,Shibata T,2007.DNA recombination-initiation plays a role in the extremely biased inheritance of yeast[rho-]mitochondrial DNA that contains the replication origin ori5.Molecular&Cellular Biology,27(3):1133-45
Liu M,Xiao ZT,Qiu YM,Qiu GG,He HQ,2016.Utilization of industry-cultivated Flammulina velutipes spent compost as substrate for Pleurotus geesteranus cultivation.Guangdong Agricultural Sciences,43(10):48-52(in Chinese)
Lu X,Zheng QX,Ye LY,Ding ZQ,Chu YR,Huang QX,Wu XP,2017.Identification and biological characteristics analysis of a wild Pleurotus pulmonarius.Genomics and Applied Biology,36(5):1997-2004(in Chinese)
Lu ZH,2008.SRAP analysis of cultivation strains of Pleurotus pulmonarius and its sibling varieties.Acta Agriculturae Jiangxi,20(11):8-10(in Chinese)
Ma HY,Bai BL,2014.The cultural techniques of Pleurotus geesteranus.Northern Horticulture,13:140-141(in Chinese)
Nguyen TK,Im KH,Choi J,Shin PG,Lee TS,2016.Evaluation of antioxidant,anti-cholinesterase,and anti-inflammatory effects of culinary mushroom Pleurotus pulmonarius.Mycobiology,44(4):291-301
Schaefer L,Engman H,Miller JB,2000.Coding sequence,chromosomal localization,and expression pattern of Nrf1:the mouse homolog of Drosophila erect wing.Mammalian Genome,11(2):104-110
Shen JW,Wang SM,Qi YC,Gao YQ,Liang ZP,Qiu LY,2007.Comparing the lentinan yield of the monokaryontic mycelia to the dikaryotic mycelia from Lentinula edodes939.Acta Horticulturae Sinica,34(4):941-946(in Chinese)
Su Rong,Shang XD,Xu Z,Tan Q,Chen MJ,Cao H,2009.Bioiogical characteristics of Flammulina velutipes self-bred progenies.Mycosystema,28(3):378-384(in Chinese)
Vanlerberghe GC,Robson CA,Yip JYH,2002.Induction of mitochondrial alternative oxidase in response to a cell signal pathway down-regulating the cytochrome pathway prevents programmed cell death.Plant Physiology,129(4):1829-1842
Wahab NAA,Abdullah N,Aminudin N,2014.Characterisation of potential antidiabetic-related proteins from Pleurotus pulmonarius(Fr.)Quél.(grey oyster mushroom)by MALDI-TOF/TOF mass spectrometry.BioMed Research International,2014(2014):131607-131607
Wang WK,Yuan WD,Fang XP,Song JL,Yan J,Lu N,2017.Comparative proteomics analysis of Pleurotus pulmonarius at different developmental phases.Journal of Zhejiang University(Agriculture&Life Sciences),43(5):527-535(in Chinese)
Weng BQ,Jiang ZH,Lin Y,2002.Effect of different kinds of composts on the nutritional evaluation of protein in Pleurotus geesteranus fruitbodies.Acta Edulis Fungi,9(2):10-13(in Chinese)
Wu SJ,Wu XJ,Chen XF,Wang CQ,Wei SY,2017.Effects of sawdust of Moringa oleifera Lam.on the yield and quality of fruit body of Pleurotus pulmonarius.Chinese Journal of Tropical Crops,38(3):482-486(in Chinese)
Xin Y,Ruan SL,Wang SH,Ma HS,Wang WK,Wang SZ,2008.Cluster analysis of ten Pleurotus geesteranus strains based on random amplification of polymorphic DNA(RAPD)and expressed sequence tag single sequence repeats(ESTSSR)markers.Acta Edulis Fungi,15(4):20-25(in Chinese)
Yang XD,Yang JH,Zhang MF,2011.Possible pathways and mechanisms of mitochondrial retrograde regulation of nuclear gene expression in Brassica juncea.Acta Horticulturae Sinica,38(S):2540(in Chinese)
Ye LY,Meng GL,Cheng B,Zhao LL,Xie F,Wu XP,2018.Mitochondrial genome sequencing and polymorphism analysis of Pleurotus pulmonarius.Mycosystema,37(9):1179-1187(in Chinese)
Ye M,Pan YJ,Ma GR,Chen YX,2001.Genetic analysis of hybrids of the positive and negative di-mon mating in Lentinula edodes.Mycosystema,20(1):94-99(in Chinese)
Zhang JL,Zhang LJ,Li L,Liu JH,Tan Q,Shang XD,2015.Inbreeding and selection of new strain Shenxiu-1 of Pleurotus pulmonarius.Microbiology,42(8):1539-1548(in Chinese)
Zhang QZ,Zhang DQ,Liu QJ,2012.Research status on interaction between nuclear genome and mitochondrial genome in tumorgenesis.Journal of Radiation Research and Radiation Processing,30(2):65-70(in Chinese)
Zhang XY,Zhang B,Xin G,Gong X,2016.Research progress on nutrition constituents,bioactivity and storage preservation of Pleurotus geesteranus.Journal of Food Safety&Quality,6:2314-2319(in Chinese)
Zhou SH,2016.Cloning and expression analysis of the Ppcsl-1gene related to change-temperature fruiting from Pleurotus pulmonarius.Master Thesis,Zhejiang Normal University,Jinhua.37-45(in Chinese)
Zhu J,Liu XR,Xie BG,Liu FY,Deng YJ,Xiong F,2009.Analysis of germplasm resources of Pleurotus geesteranus by ITS-RFLP.Journal of Fujian Agriculture and Forestry University(Natural Science Edition),38(2):186-191(in Chinese)
曹乾超,张雅芬,胡鹏,崔海峰,俞晓平,叶子弘,2017.菰黑粉菌菌丝形成相关基因UeSsk2的克隆及表达分析.中国计量学院学报,28(2):252-260
陈君琛,沈恒胜,汤葆莎,杨菁,刘韬,杨嘉金,2003.秀珍菇反季节高效栽培技术研究.中国食用菌,22(4):21-23
陈丽峰,柳君泽,2005.细胞核基因组与线粒体基因组的相互调控.中国病理生理杂志,21(8):1614-1618
陈躬国,林原,刘新锐,赵光辉,陈剑,2017.秀珍菇遗传育种研究进展.热带作物学报,38(7):1377-1381
崔亚忠,陶岚,孙晨浩,刘平,李桂华,秦庆明,2016.ABC转运蛋白BcAtm1参与灰葡萄孢分生孢子萌发及对寄主植物的侵染.植物病理学报,46(5):614-623
戴玉成,杨祝良,2008.中国药用真菌名录及部分名称的修订.菌物学报,27:801-824
高春鹏,仲来福,任翔,姜丽平,耿成燕,姚晓峰,曹军,2011.线粒体DNA缺失和功能缺失对核基因影响.中国公共卫生,27(1):50-51
顾明亮,汪业军,陈姝,张永彪,2009.线粒体基因与核基因协同表达的机制.生命的化学,29(6):803-811
江枝和,肖淑霞,雷锦桂,王义祥,翁伯琪,2009.添加外源锌对猪肚菇子实体蛋白质营养价值的影响.农业环境科学学报,28(3):449-453
况丹,2011.七种食用菌营养成分分析比较.食用菌,4:57-59
李海梅,王滢莹,高弢,徐寸发,石志琦,陈健,许晓风,2018.FgPDK1调控禾谷镰孢菌响应渗透胁迫研究.南京师大学报(自然科学版),41(1):76-82
李巧珍,吴迪,陈明杰,李玉,宋晓霞,周峰,李正鹏,杨焱,2015.猴头菌子实体不同发育阶段蛋白质营养价值评价.农产品加工,12:53-56
李维焕,吴璇,高兴喜,冯路瑶,贾玉萍,程显好,2018.十二个肺形侧耳菌株的比较试验.北方园艺,5:145-149
刘明,肖自添,邱耀明,邱桂根,何焕清,2016.利用金针菇渣栽培秀珍菇试验.广东农业科学,43(10):48-52
鲁欣,郑秋霞,叶丽云,丁智权,出怡汝,黄清雄,吴小平,2017.一株野生秀珍菇的鉴定及其生物学特性的分析.基因组学与应用生物学,36(5):1997-2004
卢政辉,2008.秀珍菇及其近缘22株菌株的SRAP分析.江西农业学报,20(11):8-10
马洪艳,白宝良,2014.秀珍菇栽培技术.北方园艺,13:140-141
申进文,王淑敏,戚元成,高玉千,梁振普,邱立友,2007.香菇939菌株单核菌丝和双核菌丝多糖产量比较.园艺学报,34(4):941-946
苏蓉,尚晓冬,徐珍,谭琦,陈明杰,曹晖,2009.金针菇自交后代生物学特性的研究.菌物学报,28(3):378-384
王伟科,袁卫东,方献平,宋吉玲,闫静,陆娜,2017.秀珍菇不同生长发育阶段蛋白质组学分析.浙江大学学报(农业与生命科学版),43(5):527-535
翁伯琦,江枝和,林勇,2002.不同培养料对秀珍菇子实体蛋白质营养评价的影响.食用菌学报,9(2):10-13
吴圣进,吴小建,陈雪凤,王灿琴,韦仕岩,2017.辣木屑对秀珍菇子实体产量与品质的影响.热带作物学报,38(3):482-486
忻雅,阮松林,王世恒,马华升,王伟科,王淑珍,2008.基于RAPD和EST-SSR标记的秀珍菇菌株聚类分析.食用菌学报,15(4):20-25
杨晓东,杨景华,张明方,2011.芥菜线粒体反向调控核基因表达的可能途径和机制研究.园艺学报,38(S):2540
叶丽云,孟国良,程冰,赵丽丽,解凡,吴小平,2018.肺形侧耳线粒体基因组多态性鉴定.菌物学报,37(9):1179-1187
叶明,潘迎捷,马桂荣,陈咏萱,2001.香菇正反双单杂交后代的遗传分析.菌物系统,20(1):94-99
张俊玲,章炉军,李亮,刘建辉,谭琦,尚晓冬,2015.自交选育秀珍菇新菌株“申秀1号”.微生物学通报,42(8):1539-1548
张晓玉,张博,辛广,宫雪,2016.秀珍菇营养成分、生物活性及贮藏保鲜的研究进展.食品安全质量检测学报,6:2314-2319
张庆召,张德钦,刘青杰,2012.肿瘤发生中核基因组与线粒体基因组的相互作用.辐射研究与辐射工艺学报,30(2):65-70
周烁红,2016.秀珍菇变温结实相关Ppcsl-1基因的克隆及表达分析.浙江师范大学硕士论文,金华.37-45
朱坚,刘新锐,谢宝贵,刘福阳,邓优锦,熊芳,2009.秀珍菇种质资源的ITS-RFLP分析.福建农林大学学报(自然科学版),38(2):186-191