基于二氨基荧光素类荧光探针的新型一氧化氮检测方法
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摘要
提出了一种基于二氨基荧光素类一氧化氮(NO)探针DAF-FM的新型高效NO检测方法。通过纳米金颗粒的添加,显著增强了探针荧光强度,同时NO检测的灵敏度得到了显著的提高。该新型荧光探针可重复使用24次以上,弥补了DAF-FM的单次使用缺陷,实现了一氧化氮的可持续重复检测。
A new and efficient nitric oxide(NO) detection method based on the DAF-FM which is a kind of NO probes was proposed. The incorporation of gold nanoparticles significantly increased the fluorescence intensity of the fluorescent probe after reacting with NO. The new fluorescent probes composed of DAF-FM and gold nanoparticles have dozens of times higher fluorescence intensity than that without gold nanoparticles does. The sensitivity of NO detection has been significantly improved. The new fluorescent probe can be reused for more than 24 times,overcoming the single-use problem of DAF-FM and achieving the sustainable reuse of commercial nitric oxide detection.
A new and efficient nitric oxide(NO) detection method based on the DAF-FM which is a kind of NO probes was proposed. The incorporation of gold nanoparticles significantly increased the fluorescence intensity of the fluorescent probe after reacting with NO. The new fluorescent probes composed of DAF-FM and gold nanoparticles have dozens of times higher fluorescence intensity than that without gold nanoparticles does. The sensitivity of NO detection has been significantly improved. The new fluorescent probe can be reused for more than 24 times,overcoming the single-use problem of DAF-FM and achieving the sustainable reuse of commercial nitric oxide detection.
引文
[1]F?RSTERMANN U,SESSA W C.Nitric oxide synthases:regulation and function[J].European Heart Journal,2012,33(7):829-837.
[2]TAYLOR D R.Advances in the clinical applications of exhaled nitric oxide measurements[J].Journal of Breath Research,2012,6(4):047102.
[3]SINGH A K,PANDITA S,CHANDRA G,et al.Role of nitric oxide in immunity[J].Wayamba Journal of Animal Science,2011.
[4]赵婷婷.新型一氧化氮荧光探针的合成与性能探究[D].上海:上海交通大学,2016.
[5]M?LLER M N,LI Q,LANCASTER Jr J R,et al.Acceleration of nitric oxide autoxidation and nitrosation by membranes[J].IUBMB Life,2007,59(4/5):243-248.
[6]CRACK J C,GREEN J,THOMSON A J,et al.Ironsulfur clusters as biological sensors:the chemistry of reactions with molecular oxygen and nitric oxide[J].Accounts of Chemical Research,2014,47(10):3196-3205.
[7]ZHAO H X,CHEN X Y,LI X T,et al.Photoinduced formation of reactive oxygen species and electrons from metal oxide-silica nanocomposite:an EPR spin-trapping study[J].Applied Surface Science,2017,416:281-287.
[8]SHIN J H,SCHOENFISCH M H.Inorganic/organic hybrid silica nanoparticles as a nitric oxide delivery scaffold[J].Chemistry of Materials,2008,20(1):239-249.
[9]PUSTELNY K,BIELANSKA J,PLONKA P M,et al.In vivo spin trapping of nitric oxide from animal tumors[J].Nitric Oxide,2007,16(2):202-208.
[10]KOJIMA H,NAKATSUBO N,KIKUCHI K,et al.Detection and imaging of nitric oxide with novel fluorescent indicators:diaminofluoresceins[J].Analytical Chemistry,1998,70(13):2446-2453.
[11]ST LAURENT C D S,MOON T C,BEFUS A D.Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate[J].Methods in Molecular Biology,2015,1220:339-345.
[12]刘杉杉.纳米金颗粒研究现状与发展前景[J].广西轻工业,2011,27(11):34-35.
[13]郑海霞,黄博能,胡君曼,等.制备特定尺寸的纳米金颗粒方法及性能表征[J].北京大学学报(自然科学版),2011,47(5):777-782.
[2]TAYLOR D R.Advances in the clinical applications of exhaled nitric oxide measurements[J].Journal of Breath Research,2012,6(4):047102.
[3]SINGH A K,PANDITA S,CHANDRA G,et al.Role of nitric oxide in immunity[J].Wayamba Journal of Animal Science,2011.
[4]赵婷婷.新型一氧化氮荧光探针的合成与性能探究[D].上海:上海交通大学,2016.
[5]M?LLER M N,LI Q,LANCASTER Jr J R,et al.Acceleration of nitric oxide autoxidation and nitrosation by membranes[J].IUBMB Life,2007,59(4/5):243-248.
[6]CRACK J C,GREEN J,THOMSON A J,et al.Ironsulfur clusters as biological sensors:the chemistry of reactions with molecular oxygen and nitric oxide[J].Accounts of Chemical Research,2014,47(10):3196-3205.
[7]ZHAO H X,CHEN X Y,LI X T,et al.Photoinduced formation of reactive oxygen species and electrons from metal oxide-silica nanocomposite:an EPR spin-trapping study[J].Applied Surface Science,2017,416:281-287.
[8]SHIN J H,SCHOENFISCH M H.Inorganic/organic hybrid silica nanoparticles as a nitric oxide delivery scaffold[J].Chemistry of Materials,2008,20(1):239-249.
[9]PUSTELNY K,BIELANSKA J,PLONKA P M,et al.In vivo spin trapping of nitric oxide from animal tumors[J].Nitric Oxide,2007,16(2):202-208.
[10]KOJIMA H,NAKATSUBO N,KIKUCHI K,et al.Detection and imaging of nitric oxide with novel fluorescent indicators:diaminofluoresceins[J].Analytical Chemistry,1998,70(13):2446-2453.
[11]ST LAURENT C D S,MOON T C,BEFUS A D.Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate[J].Methods in Molecular Biology,2015,1220:339-345.
[12]刘杉杉.纳米金颗粒研究现状与发展前景[J].广西轻工业,2011,27(11):34-35.
[13]郑海霞,黄博能,胡君曼,等.制备特定尺寸的纳米金颗粒方法及性能表征[J].北京大学学报(自然科学版),2011,47(5):777-782.