外源烯效唑对低温胁迫下薏苡幼苗的缓解效应的研究
|
摘要
为研究外源烯效唑(S3307)对薏苡幼苗在低温胁迫的缓解作用。该文以"薏辽5号"为试验材料,采用营养液水培法探讨不同质量浓度(1,3,5,7,9 mg·L~(-1))S3307对低温胁迫下薏苡幼苗性状及生理指标的影响。结果表明,低温胁迫下S3(5 mg·L~(-1))能够显著增加低温胁迫下薏苡幼苗茎粗和干重,其茎粗比低温胁迫组提高11.90%,地上地下干重分别比低温胁迫组提高13.59%,10.99%;叶宽和侧根条数分别增加7.63%,37.52%。同时5 mg·L~(-1)烯效唑组相对电导率和丙二醛比低温胁迫组分别降低23.33%,17.42%,叶片可溶性糖和脯氨酸含量分别比低温胁迫组提高17.16%,11.87%,根系可溶性糖和脯氨酸含量分别比低温胁迫组提高20.00%,33.42%。另外, S3307还能够通过改善细胞膜及叶绿体类囊体片层结构等来缓解低温胁迫对细胞超微结构的伤害。5 mg·L~(-1) S3307能通过调控薏苡幼苗生长及生理指标来增强其抗低温能力,缓解低温对幼苗所造成的伤害。
The aim of the study is to explore exogenous S3307 on alleviating low-temperature stress of coix seedlings. The coix cultivar, "No 5 Yiliao", was selected as the plant material, through nutrient solution cultivating in greenhouse, the effect of different S3307 concentrations(1, 3, 5, 7, 9 mg·L~(-1)) on coix seedlings traits and physiological indicators were explored under low-temperature stress. The results showed, under low-temperature 5 mg·L~(-1) S3307 could significantly increase coix seedlings stem diameter and biomass, which stem diameter and above-ground biomass, low-ground biomass separately were enhanced 11.90%, 13.59%, 10.99%. Leaf width and lateral root number separately were enhanced 7.63%, 37.52%. Meanwhile, addition of 5 mg·L~(-1) S3307 could significantly reduce relative conductivity and MDA, separately being reduced 23.33%, 17.42% compared to CKL. S3307 could also significantly increase soluble sugar and proline content, which leaf soluble sugar and proline content separately were enhanced 17.16%, 11.87%, which root soluble sugar and proline content separately were enhanced 20.00%, 33.42%. Additionally, S3307 could alleviate the cells destroy in ultra-structure level by improving cell membrane structure and chloroplast capsule layer structure. 5 mg·L~(-1) S3307 could enhance the low temperature tolerance of coix seedlings by regulating the growth and physiological indexes, and thus alleviate the damage caused by low-temperature to the coix seedlings.
The aim of the study is to explore exogenous S3307 on alleviating low-temperature stress of coix seedlings. The coix cultivar, "No 5 Yiliao", was selected as the plant material, through nutrient solution cultivating in greenhouse, the effect of different S3307 concentrations(1, 3, 5, 7, 9 mg·L~(-1)) on coix seedlings traits and physiological indicators were explored under low-temperature stress. The results showed, under low-temperature 5 mg·L~(-1) S3307 could significantly increase coix seedlings stem diameter and biomass, which stem diameter and above-ground biomass, low-ground biomass separately were enhanced 11.90%, 13.59%, 10.99%. Leaf width and lateral root number separately were enhanced 7.63%, 37.52%. Meanwhile, addition of 5 mg·L~(-1) S3307 could significantly reduce relative conductivity and MDA, separately being reduced 23.33%, 17.42% compared to CKL. S3307 could also significantly increase soluble sugar and proline content, which leaf soluble sugar and proline content separately were enhanced 17.16%, 11.87%, which root soluble sugar and proline content separately were enhanced 20.00%, 33.42%. Additionally, S3307 could alleviate the cells destroy in ultra-structure level by improving cell membrane structure and chloroplast capsule layer structure. 5 mg·L~(-1) S3307 could enhance the low temperature tolerance of coix seedlings by regulating the growth and physiological indexes, and thus alleviate the damage caused by low-temperature to the coix seedlings.
引文
[1] 李祥栋,潘虹,陆秀娟,等.薏苡种质的主要营养组分特征及综合评价[J].中国农业科学,2018,51(5):835.
[2] 麻继仙,杨长楷,但忠,等.50%矮壮素水剂对不同苗龄番茄幼苗的影响[J].现代农业科技,2012(4):211.
[3] 陈笑莹,宋凤斌,朱先灿,等.低温胁迫下丛枝菌根真菌对玉米幼苗形态、生长和光合的影响[J].华北核农学报,2014,29(S1):155.
[4] 甘小虎,何从亮,阎庆久,等.矮壮素、多效唑对高温季节黄瓜育苗的影响[J].蔬菜,2012(7):64.
[5] 甄红丽,苑兆和,冯立娟,等.矮壮素对大丽花生长发育和内源激素含量的影响及相关性分析[J].草业科学,2012,29(1):76.
[6] Wu C,Sun J,Zhang A,et al.Dissipation and enantioselective degradation of plant growth retardants paclobutrazol and uniconazole in open field,greenhouse,and laboratory soils[J].Environ Sci Technol,2013,47:843.
[7] 郑春芳,陈继浓,仇建标,等.烯效唑对低温胁迫下秋茄幼苗光合作用与抗氧化系统的影响[J].植物生理学报,2016,52(1):109.
[8] 姚雄,任万军,杨文钰,等.烯效唑对水稻秧苗抵御不同类型低温胁迫能力的影响[J].草业学报,2008,17(5):68.
[9] 杨建新,胡义文,杨大旗.烯效唑(S-3307)对油菜幼苗抗寒性的生理效应[J].西南农业大学学报,1994,16(3):256.
[10] 孙丽荣.S3307浸种对玉米生长发育及幼苗抗冷性的调控效应[D].长春:吉林农业大学,2002.
[11] Michael P I,Krishnaswamy M.The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings[J].Environ Exp Bot,2011,74(120):171.
[12] 李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:182.
[13] Liu Y,Fang Y,Huang M J,et al.Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) Ⅰ:transcriptome analysis of the effects of uniconazole on chlorophyll and endogenous hormone biosynthesis[J].Biotechnol Biofuels,2015,8:57.
[14] 杨文钰,徐精文,张鸿.烯效唑(S-3307)对秧苗抗寒性的影响及其作用机理研究[J].杂交水稻,2003,18(2):53.
[15] 邓凤飞,杨双龙,龚明.外源ABA对低温胁迫下小桐子幼苗脯氨酸积累及其代谢途径的影响[J].植物生理学报,2015,51(2):221.
[16] 王宁,董莹莹,苏金乐.低温胁迫下2种樟树叶片超微结构的比较[J].西北农林科技大学学报:自然科学版,2013,41(7):106.
[17] 郑国华,张贺英,钟秀容.低温胁迫下枇杷叶片细胞超微结构及膜透性和保护酶活性的变化[J].中国生态农业学报,2009,17(4):739.
[18] 肖承鸿,周涛,江维克,等.低温层积及赤霉素处理对太子参种子萌发与幼苗生长的影响[J].中国实验方剂学杂志,2013,19(15):151.
[19] 杨大旗,杨建新,胡义文.优康唑(S3307)对油菜幼苗某些生理特性的影响[J].植物生理学通讯,1994,30(3):182.
[2] 麻继仙,杨长楷,但忠,等.50%矮壮素水剂对不同苗龄番茄幼苗的影响[J].现代农业科技,2012(4):211.
[3] 陈笑莹,宋凤斌,朱先灿,等.低温胁迫下丛枝菌根真菌对玉米幼苗形态、生长和光合的影响[J].华北核农学报,2014,29(S1):155.
[4] 甘小虎,何从亮,阎庆久,等.矮壮素、多效唑对高温季节黄瓜育苗的影响[J].蔬菜,2012(7):64.
[5] 甄红丽,苑兆和,冯立娟,等.矮壮素对大丽花生长发育和内源激素含量的影响及相关性分析[J].草业科学,2012,29(1):76.
[6] Wu C,Sun J,Zhang A,et al.Dissipation and enantioselective degradation of plant growth retardants paclobutrazol and uniconazole in open field,greenhouse,and laboratory soils[J].Environ Sci Technol,2013,47:843.
[7] 郑春芳,陈继浓,仇建标,等.烯效唑对低温胁迫下秋茄幼苗光合作用与抗氧化系统的影响[J].植物生理学报,2016,52(1):109.
[8] 姚雄,任万军,杨文钰,等.烯效唑对水稻秧苗抵御不同类型低温胁迫能力的影响[J].草业学报,2008,17(5):68.
[9] 杨建新,胡义文,杨大旗.烯效唑(S-3307)对油菜幼苗抗寒性的生理效应[J].西南农业大学学报,1994,16(3):256.
[10] 孙丽荣.S3307浸种对玉米生长发育及幼苗抗冷性的调控效应[D].长春:吉林农业大学,2002.
[11] Michael P I,Krishnaswamy M.The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings[J].Environ Exp Bot,2011,74(120):171.
[12] 李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:182.
[13] Liu Y,Fang Y,Huang M J,et al.Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) Ⅰ:transcriptome analysis of the effects of uniconazole on chlorophyll and endogenous hormone biosynthesis[J].Biotechnol Biofuels,2015,8:57.
[14] 杨文钰,徐精文,张鸿.烯效唑(S-3307)对秧苗抗寒性的影响及其作用机理研究[J].杂交水稻,2003,18(2):53.
[15] 邓凤飞,杨双龙,龚明.外源ABA对低温胁迫下小桐子幼苗脯氨酸积累及其代谢途径的影响[J].植物生理学报,2015,51(2):221.
[16] 王宁,董莹莹,苏金乐.低温胁迫下2种樟树叶片超微结构的比较[J].西北农林科技大学学报:自然科学版,2013,41(7):106.
[17] 郑国华,张贺英,钟秀容.低温胁迫下枇杷叶片细胞超微结构及膜透性和保护酶活性的变化[J].中国生态农业学报,2009,17(4):739.
[18] 肖承鸿,周涛,江维克,等.低温层积及赤霉素处理对太子参种子萌发与幼苗生长的影响[J].中国实验方剂学杂志,2013,19(15):151.
[19] 杨大旗,杨建新,胡义文.优康唑(S3307)对油菜幼苗某些生理特性的影响[J].植物生理学通讯,1994,30(3):182.