正畸加力过程中外周血和脾脏中炎性单核细胞比例和CD226分子表达水平变化
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摘要
目的:观测小鼠牙齿正畸加力过程中外周血和脾脏中炎性单核细胞比例和CD226分子表达水平变化。方法:选择6～8周龄雌性C57BL小鼠,随机分为加力1 d组(n=5)、加力3 d组(n=4)、加力7 d组(n=4)和相应时间的对照组(n=3×3)。届时分离小鼠外周血和脾脏进行单个核细胞流式细胞术检测,在体式显微镜下观察牙齿移动情况;取上颌骨标本切片后行HE染色和免疫荧光染色观测破骨细胞生成情况。结果:随着加力时间延长,破骨细胞生成增多;加力1 d时,外周血中炎性单核细胞比例降低; 3 d时,外周血和脾脏中炎性单核细胞比例升高,而CD226分子表达水平均降低; 7 d时,外周血中炎性单核细胞比例较对照组升高但较加力3 d组降低,外周血和脾脏中炎性单核细胞表面CD226分子表达水平降低。结论:正畸加力过程中,小鼠外周血和脾脏中的炎性单核细胞比例和CD226分子表达水平动态变化可能影响牙齿移动过程。
Objective: To observe the inflammatory monocytes ratio(iMos) and the expression of CD226 in peripheral blood and spleen during orthodontic treatment in mice. Methods: Female C57 mice aged 6-8 weeks were randomly divided into 1(n=5), 3(n=4) and 7 d(n=4) orthodontic loading groups and corresponding control groups(n=3×3). The ratio of iMos and the expression level of CD226 in peripheral blood and spleen was detected by flow cytometry. The teeth movement was observed under a microscope. The specimens of maxilla with orthodontic teeth were stained with HE and immunofluorescent dye for the observation of osteoclast formation. Results: During orthodontic, the number of osteoclasts increased with the decrease of iMos ratio in peripheral blood in 1 day, the iMos ratio increased and the expression level of CD226 increased in 3 days,and the iMos ratio and the expression level of CD226 in spleen decreased in 7 days. Conclusion: During orthodontic stress, the ratio of iMos and the expression level of CD226 in peripheral blood and spleen may influence tooth movement.
Objective: To observe the inflammatory monocytes ratio(iMos) and the expression of CD226 in peripheral blood and spleen during orthodontic treatment in mice. Methods: Female C57 mice aged 6-8 weeks were randomly divided into 1(n=5), 3(n=4) and 7 d(n=4) orthodontic loading groups and corresponding control groups(n=3×3). The ratio of iMos and the expression level of CD226 in peripheral blood and spleen was detected by flow cytometry. The teeth movement was observed under a microscope. The specimens of maxilla with orthodontic teeth were stained with HE and immunofluorescent dye for the observation of osteoclast formation. Results: During orthodontic, the number of osteoclasts increased with the decrease of iMos ratio in peripheral blood in 1 day, the iMos ratio increased and the expression level of CD226 increased in 3 days,and the iMos ratio and the expression level of CD226 in spleen decreased in 7 days. Conclusion: During orthodontic stress, the ratio of iMos and the expression level of CD226 in peripheral blood and spleen may influence tooth movement.
引文
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[2] Reitan K.The initial tissue reaction incident to orthodontic tooth movement as related to the influence of function;an experimental histologic study on animal and human material[J].Acta Odontol Scand Suppl,1951,6:1-240.
[3] Ono T,Nakashima T.Recent advances in osteoclast biology[J].Histochem Cell Biol,2018,149(4):325-341.
[4] Krishnan V,Davidovitch Z.Cellular,molecular,and tissue- level reactions to orthodontic force[J].Am J Orthod Dentofacial Orthop,2006,129(4):469.
[5] Teitelbaum SL.Bone resorption by osteoclasts[J].Science,2000,289(5484):1504-1508.
[6] Sunderk?tter C,Nikolic T,Dillon MJ,et al.Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response[J].J Immunol,2004,172(7):4410-4417.
[7] Zeng M,Kou X,Yang R,et al.Orthodontic force induces systemic inflammatory monocyte responses[J].J Dent Res,2015,94(9):1295-1302.
[8] Vo AV,Takenaka E,Shibuya A,et al.Expression of DNAM- 1 (CD226) on inflammatory monocytes[J].Mol Immunol,2016,69:70-76.
[9] Reymond N,Imbert AM,Devilard E,et al.DNAM- 1 and PVR regulate monocyte migration through endothelial junctions[J].J Exp Med,2004,199(10):1331-1341.
[10] Yasuda H,Shima N,Nakagawa N,et al.Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis- inhibitory factor and is identical to TRANCE/RANKL[J].Proc Natl Acad Sci USA,1998,95(7):3597-3602.
[11] Lacey DL,Timms E,Tan HL,et al.Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation[J].Cell,1998,93(2):165-176.
[12] Wagner EF,Karsenty G.Genetic control of skeletal development[J].Curr Opin Genet Dev,2001,11(5):527-532.
[13] Pende D,Castriconi R,Romagnani P,et al.Expression of the DNAM- 1 ligands,Nectin- 2 (CD112) and poliovirus receptor (CD155),on dendritic cells:Relevance for natural killer- dendritic cell interaction[J].Blood,2006,107(5):2030-2036.
[14] Swirski FK,Nahrendorf M,Etzrodt M,et al.Identification of splenic reservoir monocytes and their deployment to inflammatory sites[J].Science,2009,325(5940):612-616.
[15] Sponaas AM,Freitas do Rosario AP,et al.Migrating monocytes recruited to the spleen play an important role in control of blood stage malaria[J].Blood,2009,114(27):5522-5531.