五禽戏对中年男性代谢综合征患者肠道菌群及其代谢产物的干预研究
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摘要
目的:寻找中年男性代谢综合征(metabolic syndrome,MS)发生发展相关的关键菌群,探讨五禽戏干预中年男性MS有效性的微生物学机制。方法:选取中年男性健康人和中年男性MS患者各30人,随机分为健康对照组(HCG)、健康五禽戏组(HWQXG)、MS对照组(MSCG)和MS五禽戏组(MSWQXG)。HCG和MSCG保持原有生活规律,HWQXG和MSWQXG进行6个月五禽戏干预,干预开始前1天、2个月、4个月和6个月后测试4组受试者肠道菌群、脂多糖、短链脂肪酸、胰高血糖素样肽-1、胰高血糖素样肽-2、空腹血糖、空腹胰岛素、胰岛素抵抗指数。结果:中年男性MS患者双歧杆菌、乳酸杆菌、双歧杆菌/大肠埃希菌、短链脂肪酸产生菌显著减少,且与胰岛素抵抗指数呈显著负相关,大肠埃希菌、葡萄球菌、产脂多糖的革兰氏阴性菌显著增加,且与胰岛素抵抗指数呈显著正相关。五禽戏干预可显著优化中年男性MS患者和中年男性健康人肠道菌群及其代谢产物,并呈现明显时间剂量效应。结论:五禽戏干预优化中年男性MS患者肠道菌群及其代谢产物脂多糖、短链脂肪酸、胰高血糖素样肽-1、胰高血糖素样肽-2可能是五禽戏促进中年男性MS改善的重要微生物学机制。
Objective:To find the key intestinal flora correlated with the development of middle-aged men's metabolic syndrome, and discuss the microbiological mechanism of the intervention of Wuqinxi on middle-aged men's metabolic syndrome. Methods:Sixty participants were randomly divided into four groups: health control group, health Wuqinxi group, metabolic syndrome control group and metabolic syndrome Wuqinxi group. Health control group and metabolic syndrome control group remained their normal lifestyle and no intervention was applied, whereas health Wuqinxi group and metabolic syndrome Wuqinxi group received Wuqinxi interventional training for 6 months. Intestinal flora, lipopolysaccharide, short-chain fatty acid, glucagon-like peptide-1, glucagon-like peptide-2, fasting blood glucose, fasting insulin, insulin resistance index of participants from four groups were assessed at four time points: one day before, two-, four-, and six-month after Wuqinxi interventional training. Results: The quantities of bifidobacteria, lactobacilli, bifidobacteria/scherichia coli and bacteria producing short-chain fatty acid were significantly decreased, and they were negatively correlated with insulin resistance index. The quantities of escherichia coli, staphylococcus and lipopolysaccharide were significantly increased, and they were positively correlated with insulin resistance index. The intestinal flora and its metabolites of middle-aged men who were healthy or patients with metabolic syndrome could be significantly optimized and the time-dose effect was also evident after Wuqinxi interventional training. Conclusion: It may be an important microbiological mechanism for improving middle-aged men's metabolic syndrome that the intestinal flora, lipopolysaccharide, short-chain fatty acid, glucagon-like peptide-1 and glucagon-like peptide-2 could be optimized after Wuqinxi interventional training.
Objective:To find the key intestinal flora correlated with the development of middle-aged men's metabolic syndrome, and discuss the microbiological mechanism of the intervention of Wuqinxi on middle-aged men's metabolic syndrome. Methods:Sixty participants were randomly divided into four groups: health control group, health Wuqinxi group, metabolic syndrome control group and metabolic syndrome Wuqinxi group. Health control group and metabolic syndrome control group remained their normal lifestyle and no intervention was applied, whereas health Wuqinxi group and metabolic syndrome Wuqinxi group received Wuqinxi interventional training for 6 months. Intestinal flora, lipopolysaccharide, short-chain fatty acid, glucagon-like peptide-1, glucagon-like peptide-2, fasting blood glucose, fasting insulin, insulin resistance index of participants from four groups were assessed at four time points: one day before, two-, four-, and six-month after Wuqinxi interventional training. Results: The quantities of bifidobacteria, lactobacilli, bifidobacteria/scherichia coli and bacteria producing short-chain fatty acid were significantly decreased, and they were negatively correlated with insulin resistance index. The quantities of escherichia coli, staphylococcus and lipopolysaccharide were significantly increased, and they were positively correlated with insulin resistance index. The intestinal flora and its metabolites of middle-aged men who were healthy or patients with metabolic syndrome could be significantly optimized and the time-dose effect was also evident after Wuqinxi interventional training. Conclusion: It may be an important microbiological mechanism for improving middle-aged men's metabolic syndrome that the intestinal flora, lipopolysaccharide, short-chain fatty acid, glucagon-like peptide-1 and glucagon-like peptide-2 could be optimized after Wuqinxi interventional training.
引文
[1] 孙红梅,孙强.以肠道菌群为靶点的代谢综合征运动干预研究进展[J].中国运动医学杂志,2016,35(5):490-493;505.
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[3] 刘海明.五禽戏对代谢综合征老年人认知功能的影响[J].武汉体育学院学报,2012,46(10):56-61.
[4] SAMPSON T R,DEBELIUS J W,THRON T,et al.Gut microbiota regulate motor deficits and neuroinflammation in a model of Parkinson's disease[J].Cell,2016,167(6):1469-1480.
[5] 孙红梅.健身气功·八段锦练习对老年人肠道菌群的影响[J].中国运动医学杂志,2012,31(11):973-977.
[6] 傅朝晖,王文丽,张滨.239例老年非肠道疾病患者肠道菌群状况研究[J].重庆医学,2012,41(23):2400-2402.
[7] TURNBAUGH P J,HAMADY M,YATSUNENKO T,et al.A core gut microbiome in obese and lean twins[J].Nature,2009,457(7228):480-484.
[8] 陈卫,田培郡,张程程,等.肠道菌群与人体健康的研究热点与进展[J].中国食品学报,2017,17(2):1-6.
[9] LE E,NIELSEN T,QIN J,et al.Richness of human gut microbiome correlates with metabolic markers[J].Nature,2013,500(7464):541-546.
[10] QIN J,LI Y,CAI Z,et al.A metagenome-wide association study of gut microbiota in type 2 diabetes[J].Nature,2012,490(7418):55-60.
[11] 刘金宝,王烨,王长辉,等.新疆哈萨克族正常血压人群和高血压人群肠道菌群中拟杆菌属、梭菌属结构特征分析[J].中国微生态学杂志,2010,22(5):420-422.
[12] CLARKE S F,MURPHY E F,O'SULLIVAN O,et al.Exercise and associated dietary extremes impact on gut microbial diversity[J].Gut Microbiota,2014,63(12):1913-1920.
[13] 蒋兴宇,赵霞,邹凌云,等.适度运动对人体肠道菌群结构的影响[J].第三军医大学学报,2017,39(18):1824-1831.
[14] 周涛,邱宗忠,刘巍.太极拳锻炼对肥胖老年人肠道益生菌和血脂代谢影响的相关性研究[J].山东体育学院学报,2012,28(1):62-66.
[15] CANI P D,DELZENNE N M,AMAR J,et a1.Role of gut microflora in the development of obesity and insulin resistance following high-fat diet feeding[J].Pathol Biol,2008,56(7):305-309.
[16] CANI P D,NEYRINCK A M,FAVA F,et al.Selective increases of bifidobacteriain gut microfloraim prove high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia[J].Diabetologia,2007,50(11):2374-2383.
[17] PUTHENEDAM M,WILLIAMS P H,LAKSHMI B S,et al.Modulation of tight junction barrier function by outer membrane proteins of enteropathogenic escherichia coli:role of F-actin and junctional adhesion molecule-1[J].Cell Biol Int,2007,31(8):836-844.
[18] DUCA FA,SAKAR Y,COVASA M.The modulatory role of high fat feeding on gastrointestinal signals in obesity[J].J Nutr Biochem,2013,24(10):1663-1677.
[19] 谢宁,吴小平,卢放根.肠道菌群与肥胖及相关代谢紊乱疾病炎症状态关系的研究进展[J].国际消化病杂志,2012,32(1):6-8,14.
[20] ZHOU J,MARTIN R J,TULLEY R T,et al.Dietary resistant starch upregulates total GLP-1 and PYY in a sustained day-long manner through fermentation in rodents[J].Am J Physiol Endocrinol Metab,2008,295(5):E1160-E1166.
[21] WANG Y,XU N,XI A,et al.Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microora of rats fed on high-cholesterol diet[J].Appl Microbiol Biotechnol,2009,84(2):341-347.
[22] MARTINEZ I,WALLACE G,ZHANG C,et al.Diet-induced metabolic improvements in a hamster model of hypercholesterolemia are strongly linked to alterations of the gut microbiota[J].Appl Environ Microbiol,2009,75(12):4175-4184.
[23] JAUHIAINEN T,KORPELA R.Milk peptides and blood pressure[J].J Nutr,2007,137(2):825S-829S.
[24] JAUHIAINEN T,VAPAATALO H,POUSSA T,et al.Lactobacillus helveticus fermented milk lowers blood pressure in hypertensive subjects in 24-h ambulatory blood pressure measurement[J].Am J Hypertens,2005,18(12):1600-1605.
[2] 孙红梅.五禽戏干预中年男性代谢综合征的效果及生物学机制探讨[J].中国体育科技,2015,51(4):86-92.
[3] 刘海明.五禽戏对代谢综合征老年人认知功能的影响[J].武汉体育学院学报,2012,46(10):56-61.
[4] SAMPSON T R,DEBELIUS J W,THRON T,et al.Gut microbiota regulate motor deficits and neuroinflammation in a model of Parkinson's disease[J].Cell,2016,167(6):1469-1480.
[5] 孙红梅.健身气功·八段锦练习对老年人肠道菌群的影响[J].中国运动医学杂志,2012,31(11):973-977.
[6] 傅朝晖,王文丽,张滨.239例老年非肠道疾病患者肠道菌群状况研究[J].重庆医学,2012,41(23):2400-2402.
[7] TURNBAUGH P J,HAMADY M,YATSUNENKO T,et al.A core gut microbiome in obese and lean twins[J].Nature,2009,457(7228):480-484.
[8] 陈卫,田培郡,张程程,等.肠道菌群与人体健康的研究热点与进展[J].中国食品学报,2017,17(2):1-6.
[9] LE E,NIELSEN T,QIN J,et al.Richness of human gut microbiome correlates with metabolic markers[J].Nature,2013,500(7464):541-546.
[10] QIN J,LI Y,CAI Z,et al.A metagenome-wide association study of gut microbiota in type 2 diabetes[J].Nature,2012,490(7418):55-60.
[11] 刘金宝,王烨,王长辉,等.新疆哈萨克族正常血压人群和高血压人群肠道菌群中拟杆菌属、梭菌属结构特征分析[J].中国微生态学杂志,2010,22(5):420-422.
[12] CLARKE S F,MURPHY E F,O'SULLIVAN O,et al.Exercise and associated dietary extremes impact on gut microbial diversity[J].Gut Microbiota,2014,63(12):1913-1920.
[13] 蒋兴宇,赵霞,邹凌云,等.适度运动对人体肠道菌群结构的影响[J].第三军医大学学报,2017,39(18):1824-1831.
[14] 周涛,邱宗忠,刘巍.太极拳锻炼对肥胖老年人肠道益生菌和血脂代谢影响的相关性研究[J].山东体育学院学报,2012,28(1):62-66.
[15] CANI P D,DELZENNE N M,AMAR J,et a1.Role of gut microflora in the development of obesity and insulin resistance following high-fat diet feeding[J].Pathol Biol,2008,56(7):305-309.
[16] CANI P D,NEYRINCK A M,FAVA F,et al.Selective increases of bifidobacteriain gut microfloraim prove high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia[J].Diabetologia,2007,50(11):2374-2383.
[17] PUTHENEDAM M,WILLIAMS P H,LAKSHMI B S,et al.Modulation of tight junction barrier function by outer membrane proteins of enteropathogenic escherichia coli:role of F-actin and junctional adhesion molecule-1[J].Cell Biol Int,2007,31(8):836-844.
[18] DUCA FA,SAKAR Y,COVASA M.The modulatory role of high fat feeding on gastrointestinal signals in obesity[J].J Nutr Biochem,2013,24(10):1663-1677.
[19] 谢宁,吴小平,卢放根.肠道菌群与肥胖及相关代谢紊乱疾病炎症状态关系的研究进展[J].国际消化病杂志,2012,32(1):6-8,14.
[20] ZHOU J,MARTIN R J,TULLEY R T,et al.Dietary resistant starch upregulates total GLP-1 and PYY in a sustained day-long manner through fermentation in rodents[J].Am J Physiol Endocrinol Metab,2008,295(5):E1160-E1166.
[21] WANG Y,XU N,XI A,et al.Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microora of rats fed on high-cholesterol diet[J].Appl Microbiol Biotechnol,2009,84(2):341-347.
[22] MARTINEZ I,WALLACE G,ZHANG C,et al.Diet-induced metabolic improvements in a hamster model of hypercholesterolemia are strongly linked to alterations of the gut microbiota[J].Appl Environ Microbiol,2009,75(12):4175-4184.
[23] JAUHIAINEN T,KORPELA R.Milk peptides and blood pressure[J].J Nutr,2007,137(2):825S-829S.
[24] JAUHIAINEN T,VAPAATALO H,POUSSA T,et al.Lactobacillus helveticus fermented milk lowers blood pressure in hypertensive subjects in 24-h ambulatory blood pressure measurement[J].Am J Hypertens,2005,18(12):1600-1605.