霍山石斛内生真菌及其次生代谢产物研究进展
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摘要
通过调研国内外相关文献,对已分离鉴定霍山石斛(Dendrobium huoshanense)内生真菌的种类、部分内生真菌次生代谢产物与其宿主植物的相互作用关系进行综述。结果表明,现已分离鉴定的内生真菌有41种,未分离但已鉴定到种的内生真菌有11种,种类还很有限;从这些内生真菌的次生代谢产物中已分离鉴定74个化合物,但大多数与霍山石斛的活性物质及重要生理功能关联较小;部分内生真菌如Acremonium sp.(枝顶孢霉)和Lecanicillium aphanocladii(丝枝蜡蚧霉菌),有促进霍山石斛生长或拮抗植物病原菌的潜力。
This study is mainly to review the species of endophytic fungi in Dendrobium huoshanense, the secondary metabolites of some fungi strains, and the relationship between some endophytic fungi and their host plant based on the relevant literatures at home and abroad. As the results, endophytic fungi that have been cultured and identified were 41 species, the others were uncultured but identified in species level were 11 species. These results in generally suggest that the identified species are limited. In total, 74 compounds have been isolated from some endophytic fungi of D. huoshanens, but most of them are rarely associated with the important physiological functions and active substances of D. huoshanens. Several endophytic fungi, such as Acremonium sp and Lecanicillium aphanocladii,have the potentials to promote the growth of D. huoshanens, or to inhibit the plant pathogen.
This study is mainly to review the species of endophytic fungi in Dendrobium huoshanense, the secondary metabolites of some fungi strains, and the relationship between some endophytic fungi and their host plant based on the relevant literatures at home and abroad. As the results, endophytic fungi that have been cultured and identified were 41 species, the others were uncultured but identified in species level were 11 species. These results in generally suggest that the identified species are limited. In total, 74 compounds have been isolated from some endophytic fungi of D. huoshanens, but most of them are rarely associated with the important physiological functions and active substances of D. huoshanens. Several endophytic fungi, such as Acremonium sp and Lecanicillium aphanocladii,have the potentials to promote the growth of D. huoshanens, or to inhibit the plant pathogen.
引文
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[2]陶弘景.名医别录[M].第1版,北京:人民卫生出版社,1986:29.TAO H J. Mingyi Bielu[M]. 1st Ed. Beijing:People’s Medical Publishing House,1986:29(Ch).
[3]李时珍.本草纲目[M].第1版,北京:人民卫生出版社,1982:1383.LI S Z. Bencao Gangmu[M]. 1st Ed. Beijing:People’s Medical Publishing House,1982:1383(Ch).
[4]赵学敏.本草纲目拾遗[M].第2版,北京:中国中医药出版社,2007:71.ZHAO X M. Bencao Gangmu Shiyi[M]. 2nd Ed. Beijing:Chinese Press of Traditional Chinese Medicine,2007:71(Ch).
[5] WU K G,LI T H,CHEN C J,et al. A pilot study evaluating the clinical and immunomodulatory effects of an orally administered extract of Dendrobium huoshanense in children with moderate to severe recalcitrant atopic dermatitis[J]. International Journal of Immunopathology and Pharmacology,2011,24(2):367-375. DOI:10.1177/039463201102400210.
[6] LUO J P,DENG Y Y,ZHA X P. Mechanism of polysaccharides from Dendrobium huoshanense on streptozotocininduced diabetic cataract[J]. Pharmaceutical Biology,2008,46(4):243-249. DOI:10.1080/13880200701739397.
[7] GE J C,ZHA X Q,NIE C Y,et al. Polysaccharides from Dendrobium huoshanense stems alleviates lung inflammation in cigarette smoke-induced mice[J]. Carbohydrate Polymers,2018,189:289-295. DOI:10.1016/j.carbpol.2018.02.054.
[8] ZHA X Q,DENG Y Y,LI X L,et al. The core structure of a Dendrobium huoshanense polysaccharide required for the inhibition of human lens epithelial cell apoptosis[J]. Carbohydrate Polymers,2017,155:252-260. DOI:10.1016/j.carbpol.2016.08.087.
[9] XIE S Z,GE J C,LI F,et al. Digestive behavior of Dendrobium huoshanense polysaccharides in the gastrointestinal tracts of mice[J]. International Journal of Biological Macromolecules,2018,107:825-832. DOI:10.1016/j.ijbiomac.2017.09.047.
[10]中国科学院植物研究所.中国珍稀濒危植物名录(汇总)[EB/OL].[2018-10-01]. http://rep.iplant.cn/protlist.Institute of Botany,CAS. List of China’s Rare and Endangered Plant Species(Summary)[EB/OL].[2018-10-01]. http://rep.iplant.cn/protlist(Ch).
[11] DENG Z J,CAO X. Fungal endophytes and their interactions with plants in phytoremediation:A review[J]. Chemosphere,2017,168:1100-1106. DOI:10.1016/j. chemosphere.2016.10.097.
[12]王娣,贾书华,张兆轩,等.霍山石斛内生真菌分离、培养及其促生作用的初步研究[J].菌物研究,2007,5(2):84-88. DOI:10.13341/j.jfr.2007.02.010.WANG D,JIA S H,ZHANG Z X,et al. Isolation and culture of an endophytic fungus associated with Dendrobium huoshanense and its effects on the growth of plantlets[J]. Journal of Fungal Research,2007,5(2):84-88.DOI:10.13341/j.jfr.2007.02.010(Ch).
[13]王娣.霍山石斛内生真菌的分离及其促生作用的研究[D].合肥:安徽农业大学,2007.WANG D. Isolation and Culture of Endophytic Fungi Associated with D. Huoshanense and Its Effects on the Growth of Plantlets[D]. Hefei:Anhui Agricultural University,2007(Ch).
[14]梁益敏,郁阳,麻兵继,等.一种人工栽培霍山石斛内生真菌的分离鉴定及其组织分布[J].安徽中医药大学学报,2015,34(6):75-78. DOI:10.3969/j.issn.2095-7246.2015.06024.LIANG Y M,YU Y,MA B J,et al. A method to separate and identify the endophytic fungi of Dendrobium huoshanense and their organization distribution[J]. Journal of Anhui University of Chinese Medicine,2015,34(6):75-78. DOI:10.3969/j. issn. 2095-7246.2015.06.024(Ch).
[15]郁阳.不同栽培环境下霍山石斛内生真菌生物多样性的研究[D].合肥:安徽中医药大学,2017.YU Y. Study on Biodiversity of Endophytic Fungi from Dendrobium Huoshanense under Different Cultivation Habitats[D]. Hefei:Anhui University of Traditional Chinese Medicine,2017(Ch).
[16]闵长莉,闵运江,徐慧欣,等.霍山石斛内生真菌的分离及其活性菌株的鉴定[J].天然产物研究与开发,2018,30(6):968-973. DOI:10.16333/j.1001-6880.2018.6.009.MIN C L,MIN Y J,XU H X,et al. Isolation and identification of an endophytic fungus from Dendrobium huoshanense[J]. Natural Product Research and Development,2018,30(6):968-973(Ch). DOI:10.16333/j. 1001-6880.2018.6.009.
[17]张培良,王国凯,梁益敏,等.利用GSS高通量测序技术分析不同生境下霍山石斛内生真菌群落的分布[J].安徽中医药大学学报,2018,37(1):77-81. DOI:10.3969/j.issn.2095-7246.2018.01.022.ZHANG P L,WANG G K,LI Y M,et al. Using GSS high-throughput sequencing technology to analyze the endophytic fungus from Dendrobium huoshanense under different habitat[J]. Journal of Anhui University of Chinese Medicine,2018,37(1):77-81. DOI:10.3969/j. issn.2095-7246.2018.01.022(Ch).
[18] DISSANAYAKEL A J,URAHONG W,WUBET T,et al. Direct comparison of culture-dependent and cultureindependent molecular approaches reveal the diversity of fungal endophytic communities in stems of grapevine(Vitis vinifera)[J]. Fungal Diversity,2018,90(1):85-107. DOI:10.1007/s13225-018-0399-3.
[19] JAYAWARDENA R S,URAHONG W,ZHANG W,et al. Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches[J]. Fungal Diversity,2018,90(1):1-84.DOI:10.1007/s13225-018-0398-4.
[20] STONE J K,BACON C W,WHITE J F. An Overview of Endophytic Microbes:Endophytism Defined[M].New York:Marcel Dekker Inc,2000:17-44.
[21] GANGADEVI V,MUTHMARY J. Taxol production by Pestalotiopsis terminaliae,an endophytic fungus of Terminalia arjuna(arjun tree)[J]. Biotechnology and Applied Biochemistry, 2009,52(1):9-15. DOI:10.1042/BA20070243.
[22]张玲琪,郭波,李海燕,等.长春花内生真菌的分离及其发酵产生药用成分的初步研究[J].中草药,2000,31(11):805-807. DOI:10.3321/j. issn:0253-2670.2000.11.003.ZHANG L Q,GUO B,LI H Y,et al. Preliminary study on the isolation of endophytic fungus of Catharanthus roseus and its fermentation to produce products of therapeutic value[J]. Chinese Traditional and Herbal Drugs,2000,31(11):805-807. DOI:10.3321/j. issn:0253-2670.2000.11.003(Ch).
[23]李俊峰,刘文洪,张贝贝,等.铁皮石斛内生菌的分离及代谢产物活性的初步研究[J].中华中医药杂志,2016,31(3):970-974.LI J F,LIU W H,ZHANG B B,et al. Preliminary study on separation of endophytes from Dendrobium officinale and activity of metabolites[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2016,31(3):970-974(Ch).
[24]朱媚玲,吕炜峰,高向东.金钗石解内生菌CPU009的诱变及其次级代谢产物的纯化[J].药物生物技术,2011,18(5):399-402. DOI:10.19526/j.cnki.1005-8915.2011.05.006.ZHU M L,LüL F,GAO X D. The mutagenesis of endophytic fungi CPU009 from Dendrobium nobile and the purification of its secondary metabolites[J]. Pharmaceutical Biotechnology,2011,18(5):399-402.DOI:10.19526/j.cnki.1005-8915.2011.05.006(Ch).
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