HPLC法小鼠体内奋乃静缓释微球药动力学研究
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摘要
建立小鼠体内奋乃静血药浓度液相分析方法,研究聚乙二醇修饰奋乃静缓释微球小鼠体内药动力学。采用高效液相色谱法,色谱柱为Diamonsil C18柱(250mm×4.6mm,5μm);流动相pH为3.4的缓冲液(500mL双蒸馏水中加入2mL三乙胺,用冰醋酸调节pH为3.4)-甲醇(32:68);流速0.8mL/min;柱温30℃;检测波长258nm;进样量20μL;以邻苯二甲酸二乙酯为内标物,测定小鼠体内血液中奋乃静的浓度。该方法分离度和专属性好,灵敏度高,重复性好,操作快速便捷。用聚乙二醇修饰奋乃静制备微球制剂,进行了小鼠体内药动力学研究,结果表明,聚乙二醇修饰奋乃静缓释微球制剂在小鼠体内缓释效果良好。
In this paper,a liquid phase analysis method was established to determine blood concentration of perphenazine in mice,and the pharmacokinetics of sustained release microspheres of PEG modified perphenazine in mice was studied.During this research,high performance liquid chromatography was used,and the chromatographic column was Diamonsil C18(250 mm x 4.6 mm,5μm);mobile phase was buffer solution with pH as 3.4(Add 2 mL triethylamine to 500 mL ultrapure water and adjust pH as 3.4 with glacial acetic acid)-methanol(32:68);flow rate was 0.8 mL/min,column temperature was 30 degrees,detection wavelength was 258 nm,and the sampling volume was 20μL.Diethyl phthalate was used as internal standard to determine concentration of perphenazine in blood of mice.The method had good separation and specificity,high sensitivity,good repeatability and fast and convenient operation.The results showed the sustained release microspheres of perphenazine modified by polyethylene glycol possessed good sustained release effect in mice.
In this paper,a liquid phase analysis method was established to determine blood concentration of perphenazine in mice,and the pharmacokinetics of sustained release microspheres of PEG modified perphenazine in mice was studied.During this research,high performance liquid chromatography was used,and the chromatographic column was Diamonsil C18(250 mm x 4.6 mm,5μm);mobile phase was buffer solution with pH as 3.4(Add 2 mL triethylamine to 500 mL ultrapure water and adjust pH as 3.4 with glacial acetic acid)-methanol(32:68);flow rate was 0.8 mL/min,column temperature was 30 degrees,detection wavelength was 258 nm,and the sampling volume was 20μL.Diethyl phthalate was used as internal standard to determine concentration of perphenazine in blood of mice.The method had good separation and specificity,high sensitivity,good repeatability and fast and convenient operation.The results showed the sustained release microspheres of perphenazine modified by polyethylene glycol possessed good sustained release effect in mice.
引文
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[6]张杨慧,蒋孟虹,秦峰.柱后衍生化-HPLC法测定硫酸卡那霉素注射液及滴眼液的含量[J].中国抗生素杂志,2018,26(10):1-6.
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[10]张纯刚,于琛琛,程兰.白藜芦醇壳聚糖缓释微球在大鼠体内的药动力学研究[J].中南药学,2018,16(9):1210-1213.
[2]冯志云,吴敏,朱昌平,等.聚乙二醇共价交联海藻酸钠凝胶制备及其药物缓释性能[J].材料科学与工程学报,2015,33(3):369-371.
[3]魏立华,陈芸生.HPLC法测定茯珍儿科胶囊中茯苓酸含量[J].福建中医药,2017,48(6):35-36.
[4]李俊强,吕艳妮.HPLC法测定天麻清脑胶囊中天麻素的含量[J].天津药学,2017,29(6):20-22.
[5]董丽红,韦建玉,胡亚杰.固相萃取-高效液相色谱法测定烤烟中角鲨烯含量[J].山东农业科学,2017,49(12):130-134.
[6]张杨慧,蒋孟虹,秦峰.柱后衍生化-HPLC法测定硫酸卡那霉素注射液及滴眼液的含量[J].中国抗生素杂志,2018,26(10):1-6.
[7]郭佩佩,姚宇秀,王艳菲.高效液相色谱法检测乳中β-内酰胺酶抑制剂—克拉维酸[J].中国食品学报,2018,18(8):254-258.
[8]赵燕娜,蔡畅,张勤秀.大鼠血浆中利托那韦的HPLC法测定及其药动力学研究[J].聊城大学学报(自然科学版),2018,31(2):1-7.
[9]陈彦,陈刚,余慧珍.大麻素受体2对肥胖小鼠体质量、血脂及炎症因子的影响[J].中国生化药物杂志,2014,34(8):96-98.
[10]张纯刚,于琛琛,程兰.白藜芦醇壳聚糖缓释微球在大鼠体内的药动力学研究[J].中南药学,2018,16(9):1210-1213.