基于形态学及线粒体COI基因序列的河南省新纪录种兴凯鱊近似种的鉴定
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摘要
2017年河南省鱼类资源调查队在平顶山市叶县境内沙河段进行鱼类资源调查过程中,采获3尾鱊属Acheilognathus鱼类,经形态特征鉴定其为兴凯鱊近似种(Acheilognathus aff. chankaensis),并通过分析线粒体COI基因来验证形态学的鉴定结果,以弥补传统形态学分析方法中可能由于形态差异较小、形态特征主观判断较强等原因导致的分类鉴定结果差距较大的问题,从而实现对兴凯鱊近似种的有效识别,后查阅文献该种为河南省新记录种,其主要鉴别形态特征为:口端位,口角有短凸状须或无须,口角与眼下缘水平线接近,第二不分枝鳍条较长,体侧纵带向前延伸超背鳍起点.通过与鱊属其他鱼类分析比较,基于MEGA 6软件中的常用KP2模型建立NJ系统发育树,结果显示,采集到的样本单独聚为一支构成一个单系类群且具有较高的支持率.标本保存于河南师范大学水产学院鱼类标本室,就相关内容进行详细报道.
During the fish resource investigation of Henan province in 2017, we captured three fish(Acheilognathus) in the Shahe River of Yexian County section, Pingdingshan City, which were identified by morphological characteristics as Acheilognathus aff. Chankaensis. Meanwhile, we analyzed the mitochondrial COI gene to confirm the morphological identification results to compensate for the difficulty of classification in traditional morphological analysis methods due to small morphological differences and subjective judgment for morphological characters, thus achieving efficient recognition of Acheilognathus aff. chankaensis. After widely reviewing the literature we conformed that Acheilognathus aff. chankaensis is a new recorded species in Henan province. And its main identification morphological features are as follows. The direction of the mouth points straight ahead. Short convex whiskers or no whiskers at the corners of the mouth and the corners of the mouth are close to the lower edge of the eyes. The second unbranched dorsal fin is longer than other unbranched dorsal fin. The longitudinal band on the side of the body extends forward past the base of the dorsal fin. Based on the analysis of other fishes in Acheilognathus, the NJ phylogenetic tree was established based on the K2 P model with MEGA 6 software. The results showed that the collected samples were clustered into one monophyletic group with high support value. The specimens of Acheilognathus aff. chankaensis are now kept in the fish specimens laboratory of the fisheries college of Henan normal university. This paper will report the relevant content.
During the fish resource investigation of Henan province in 2017, we captured three fish(Acheilognathus) in the Shahe River of Yexian County section, Pingdingshan City, which were identified by morphological characteristics as Acheilognathus aff. Chankaensis. Meanwhile, we analyzed the mitochondrial COI gene to confirm the morphological identification results to compensate for the difficulty of classification in traditional morphological analysis methods due to small morphological differences and subjective judgment for morphological characters, thus achieving efficient recognition of Acheilognathus aff. chankaensis. After widely reviewing the literature we conformed that Acheilognathus aff. chankaensis is a new recorded species in Henan province. And its main identification morphological features are as follows. The direction of the mouth points straight ahead. Short convex whiskers or no whiskers at the corners of the mouth and the corners of the mouth are close to the lower edge of the eyes. The second unbranched dorsal fin is longer than other unbranched dorsal fin. The longitudinal band on the side of the body extends forward past the base of the dorsal fin. Based on the analysis of other fishes in Acheilognathus, the NJ phylogenetic tree was established based on the K2 P model with MEGA 6 software. The results showed that the collected samples were clustered into one monophyletic group with high support value. The specimens of Acheilognathus aff. chankaensis are now kept in the fish specimens laboratory of the fisheries college of Henan normal university. This paper will report the relevant content.
引文
[1] 王景龙,陈启豪,陆文泽,等.河南省淮河流域鳑(鮍)亚科5种鱼形态差异分析[J].水生生物学报,2019,43(1):127-136.
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[5] 朱玉蓉,刘焕章.大鳍鱊基于细胞色素 b 基因序列的遗传变异及生物地理过程[J].水生生物学报,2006,30(2):134-140.
[6] 李琳琳.长江以南及台湾高体鳑鲏的亲缘地理研究[D].上海:上海海洋大学,2016.
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[9] Bogutskay N G ,Komlev A M.Some new date to morphology of Rhodeus sericeus (Cyprinidae,Acheilognathinae) and a description of a new species,Rhodeus colchicus,from west Transcaucasia[J],Proc Zoo INST,2001,287:81-97.
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[13] 伍献文.中国鲤科鱼类志(上)[M].上海:上海科学技术出版社,1964:202-225.
[14] 新乡师院生物系.河南鱼类志[M].郑州:河南科技出版社,1984:69-80.
[15] Bunholi I V,Ferrette B L D S,Biasi J B D,et al.The fishing and illegal trade of the angelshark:DNA barcoding against misleading identifications[J].Fisheries Research,2018,206:193-197.
[16] Na Wu,Zhang N,Cao M,et al.The identification analysis of fish eggs from lagoon of Yongshu Reef based on DNA barcoding technology[J].Freshwater Fisheries,2018(2):51-57.
[17] Cawthorn D M,Steinman H A,Witthuhn R C.DNA barcoding reveals a high incidence of fish species misrepresentation and substitution on the South African market[J].Food Research International,2012,46(1):30-40.
[18] Cutarelli A,Amoroso M G,De Roma A,et al.Italian market fish species identification and commercial frauds revealing by DNA sequencing[J].Food Control,2014,37(1):46-50.
[19] 许涛清,李仲辉.河南鱼类补遗[J].河南师范大学学报(自然科学版),1991,19(1):81-83.
[20] Triantafyllidis A,Bobori D,Koliamitra C,et al.DNA barcoding analysis of fish species diversity in four north Greek lakes[J].Mitochondrial DNA,2011,22(S1):37-42.
[21] Jeon H B,Anderson D,Won H,et al.Taxonomic characterization of Tanakiar,species (Acheilognathidae) using DNA barcoding analyses[J].Mitochondrial DNA Part A,2017:29(6):964-973.
[22] Chen W T,Ma X H,Shen Y J,et al.The fish diversity in the upper reaches of the Salween River,Nujiang River,revealed by DNA barcoding[J].Sci Rep,2015,5:17437.
[23] Sun B,Chang Y M,Su B F,et al.DNA Barcoding Analysis of Three Rhodeus Fishes in Heilongjiang River[J].Genomics and Applied Biology,2018,37(12):5220-5225.
[24] 王以康.鱼类分类学[M].北京:科技卫生出版社,1958:195-210.
[25] 刘建康.鱼类学论文集:第四集[C].北京:科学出版社,1985:22-25.
[26] 李国良.中国鳑鮍亚科分类的研究[C]//天津:天津自然博物馆论文集,1990:80-83.
[27] Jordan D S ,Thompson W F .Record of the fishes obtained in Japan in 1911[J].Mem Carneg Mas,1914,6:205-313.
[28] Matsubara K.Morhology and Hierarchy of Fishes,Part 1[M].Tokyo:Ishizaki Shoten,1955.
[29] Uchida K.The fishes of Tyosen (Korea)[J].Bull Fish Exp Sta,Government-G eneral of Tyosen,1939,6:18-43.
[30] Choi K C,Jeon S R,Kim I S,et al.Coloured illustrations of the freshwater fishes of Korea (in Korean with English abstract)[J].Seoul:Hyangmun Sa,1990,30-47.
[2] 杨晴.鱊亚科鱼类分类整理及分子系统发育研究[D].武汉:华中农业大学,2010.
[3] 王尚红.鳑鲏亚科三种鱼类线粒体全基因组测定及其比较基因组学分析[D].南昌:南昌大学,2015.
[4] 陈欣.我国鳑鮍亚科鱼类遗传多样性及系统发育研究[D].新乡:河南师范大学,2011.
[5] 朱玉蓉,刘焕章.大鳍鱊基于细胞色素 b 基因序列的遗传变异及生物地理过程[J].水生生物学报,2006,30(2):134-140.
[6] 李琳琳.长江以南及台湾高体鳑鲏的亲缘地理研究[D].上海:上海海洋大学,2016.
[7] 陈宜瑜.中国动物志(硬骨鱼纲鲤形目中卷)[M].北京:科学技术出版社,1998:413-454.
[8] Berg L S.Freshwater Fishes of the U.S.S.R.and Adjacent Countries,4-th ed,Moscow-leningrad:Izd[J].Acad Nausk SSSR,1949,2:469-925.
[9] Bogutskay N G ,Komlev A M.Some new date to morphology of Rhodeus sericeus (Cyprinidae,Acheilognathinae) and a description of a new species,Rhodeus colchicus,from west Transcaucasia[J],Proc Zoo INST,2001,287:81-97.
[10] Lelek A.Freshwater Fishes of Europe,Threatened Fishes of Europe [M].Wiesbaden:Anla Verlag,1987.
[11] Arai R.Fish systematics and cladistics.in:Uyeno T,Okiyama M eds,Ichthyology Current [M].Tokoyo:Askura Shoten,1988.
[12] Arai R,Akai Y.Acheilognathus melanogaster,a senior synonym of A.moriokae,with a revision of the genera of the subfamily Acheilognathinae (Cypriniformes,Cyprinidae)[J].Bull Natl Sci Mus TokyoSer A,1988,14:199-213.
[13] 伍献文.中国鲤科鱼类志(上)[M].上海:上海科学技术出版社,1964:202-225.
[14] 新乡师院生物系.河南鱼类志[M].郑州:河南科技出版社,1984:69-80.
[15] Bunholi I V,Ferrette B L D S,Biasi J B D,et al.The fishing and illegal trade of the angelshark:DNA barcoding against misleading identifications[J].Fisheries Research,2018,206:193-197.
[16] Na Wu,Zhang N,Cao M,et al.The identification analysis of fish eggs from lagoon of Yongshu Reef based on DNA barcoding technology[J].Freshwater Fisheries,2018(2):51-57.
[17] Cawthorn D M,Steinman H A,Witthuhn R C.DNA barcoding reveals a high incidence of fish species misrepresentation and substitution on the South African market[J].Food Research International,2012,46(1):30-40.
[18] Cutarelli A,Amoroso M G,De Roma A,et al.Italian market fish species identification and commercial frauds revealing by DNA sequencing[J].Food Control,2014,37(1):46-50.
[19] 许涛清,李仲辉.河南鱼类补遗[J].河南师范大学学报(自然科学版),1991,19(1):81-83.
[20] Triantafyllidis A,Bobori D,Koliamitra C,et al.DNA barcoding analysis of fish species diversity in four north Greek lakes[J].Mitochondrial DNA,2011,22(S1):37-42.
[21] Jeon H B,Anderson D,Won H,et al.Taxonomic characterization of Tanakiar,species (Acheilognathidae) using DNA barcoding analyses[J].Mitochondrial DNA Part A,2017:29(6):964-973.
[22] Chen W T,Ma X H,Shen Y J,et al.The fish diversity in the upper reaches of the Salween River,Nujiang River,revealed by DNA barcoding[J].Sci Rep,2015,5:17437.
[23] Sun B,Chang Y M,Su B F,et al.DNA Barcoding Analysis of Three Rhodeus Fishes in Heilongjiang River[J].Genomics and Applied Biology,2018,37(12):5220-5225.
[24] 王以康.鱼类分类学[M].北京:科技卫生出版社,1958:195-210.
[25] 刘建康.鱼类学论文集:第四集[C].北京:科学出版社,1985:22-25.
[26] 李国良.中国鳑鮍亚科分类的研究[C]//天津:天津自然博物馆论文集,1990:80-83.
[27] Jordan D S ,Thompson W F .Record of the fishes obtained in Japan in 1911[J].Mem Carneg Mas,1914,6:205-313.
[28] Matsubara K.Morhology and Hierarchy of Fishes,Part 1[M].Tokyo:Ishizaki Shoten,1955.
[29] Uchida K.The fishes of Tyosen (Korea)[J].Bull Fish Exp Sta,Government-G eneral of Tyosen,1939,6:18-43.
[30] Choi K C,Jeon S R,Kim I S,et al.Coloured illustrations of the freshwater fishes of Korea (in Korean with English abstract)[J].Seoul:Hyangmun Sa,1990,30-47.